##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146603_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3314300
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.861038530006336	31.0	31.0	34.0	27.0	34.0
2	30.944959720001208	31.0	31.0	34.0	27.0	34.0
3	31.13954138128715	31.0	31.0	34.0	27.0	34.0
4	34.66145973508735	37.0	35.0	37.0	31.0	37.0
5	34.645439459312676	37.0	35.0	37.0	31.0	37.0
6	34.72762966538937	37.0	35.0	37.0	31.0	37.0
7	34.547203029297286	37.0	35.0	37.0	30.0	37.0
8	34.55876172947531	37.0	35.0	37.0	30.0	37.0
9	36.103939293365116	39.0	37.0	39.0	30.0	39.0
10-11	36.12823416709411	39.0	37.0	39.0	30.0	39.0
12-13	36.10194852608394	39.0	37.0	39.0	30.0	39.0
14-15	37.35015237003289	40.0	38.0	41.0	31.0	41.0
16-17	37.345560450170474	40.0	38.0	41.0	31.0	41.0
18-19	37.33581978094922	40.0	37.5	41.0	31.0	41.0
20-21	37.4587463416106	40.0	38.0	41.0	31.0	41.0
22-23	37.44595480191896	40.0	38.0	41.0	31.0	41.0
24-25	37.45892345291615	40.0	37.5	41.0	30.0	41.0
26-27	37.523173068219535	40.0	37.5	41.0	30.5	41.0
28-29	37.50790468575566	40.0	37.0	41.0	30.0	41.0
30-31	37.47789457804061	40.0	37.0	41.0	30.0	41.0
32-33	37.45550146335576	40.0	37.0	41.0	30.0	41.0
34-35	37.40782276800531	40.0	37.0	41.0	30.0	41.0
36-37	37.33131913224512	40.0	37.0	41.0	30.0	41.0
38-39	37.2710777539752	40.0	37.0	41.0	30.0	41.0
40-41	37.21947681259994	40.0	37.0	41.0	30.0	41.0
42-43	37.05752240292068	40.0	36.5	41.0	30.0	41.0
44-45	36.911961801888786	40.0	36.0	41.0	30.0	41.0
46-47	36.79963069124702	40.0	36.0	41.0	29.5	41.0
48-49	36.691747427812814	39.5	35.5	41.0	29.0	41.0
50-51	36.562705699544395	39.0	35.0	41.0	29.0	41.0
52-53	36.440336270102286	39.0	35.0	41.0	29.0	41.0
54-55	36.329350843315325	39.0	35.0	41.0	29.0	41.0
56-57	36.15149066167818	39.0	35.0	40.5	28.0	41.0
58-59	35.969520260688526	38.5	35.0	40.0	28.0	41.0
60-61	35.76814772350119	38.0	34.5	40.0	28.0	41.0
62-63	35.548334037353285	38.0	34.0	40.0	28.0	41.0
64-65	35.27129635217089	37.0	34.0	40.0	28.0	41.0
66-67	35.04623661104909	37.0	34.0	40.0	27.5	41.0
68-69	34.80804921099478	36.0	34.0	39.0	27.0	41.0
70-71	34.546445101529734	36.0	34.0	39.0	27.0	41.0
72-73	34.22130072715204	35.0	33.0	39.0	26.0	40.5
74-75	33.90025269287632	35.0	33.0	38.0	26.0	40.0
76-77	33.63627311951242	35.0	33.0	37.0	26.0	39.0
78-79	33.33177216908548	35.0	33.0	37.0	26.0	39.0
80-81	33.098031107624536	35.0	33.0	36.0	26.0	39.0
82-83	32.8302766798419	35.0	33.0	36.0	26.0	37.5
84-85	32.62571432881755	35.0	33.0	35.5	25.5	37.0
86-87	32.42131355037263	35.0	32.0	35.0	25.0	37.0
88-89	32.24674229852457	35.0	32.0	35.0	25.0	36.0
90-91	32.10387593156926	35.0	32.0	35.0	25.0	36.0
92-93	31.959045348942464	35.0	32.0	35.0	25.0	36.0
94-95	31.84459599312072	35.0	32.0	35.0	25.0	35.5
96-97	31.764560691548745	35.0	32.0	35.0	24.0	35.0
98-99	31.663345804543944	35.0	32.0	35.0	24.0	35.0
100	30.46648764444981	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47471899997603373
1101	2	0.48786326869010566
1101	3	0.48968420598306395
1101	4	0.5422868906022629
1101	5	0.4655862114817708
1101	6	0.5500159708573946
1101	7	0.623612365238948
1101	8	0.5674252277101317
1101	9	0.6971658496219533
1101	10-11	0.6948570175658872
1101	12-13	0.7300401443500633
1101	14-15	0.8418383183134495
1101	16-17	0.8951592749102346
1101	18-19	0.7852739662702106
1101	20-21	0.8298618402650249
1101	22-23	0.8520356254718564
1101	24-25	0.8763530923612279
1101	26-27	0.8139589109697596
1101	28-29	0.7669575041777463
1101	30-31	0.8188142348079879
1101	32-33	0.8440568438013614
1101	34-35	0.8230845433495588
1101	36-37	0.7891714305340329
1101	38-39	0.8139809141437695
1101	40-41	0.785975026730533
1101	42-43	0.8198360333546262
1101	44-45	0.7458413983474586
1101	46-47	0.6997179281525518
1101	48-49	0.7033157557371155
1101	50-51	0.6708292347209195
1101	52-53	0.6622985530984948
1101	54-55	0.695214023402464
1101	56-57	0.7440702379609689
1101	58-59	0.7812086250935337
1101	60-61	0.7120611968888326
1101	62-63	0.7061856792342951
1101	64-65	0.7164328944304614
1101	66-67	0.7941608411343992
1101	68-69	0.6611942197151706
1101	70-71	0.6091084423462334
1101	72-73	0.6302341823034396
1101	74-75	0.5823859117575267
1101	76-77	0.593027127062868
1101	78-79	0.5748791383846878
1101	80-81	0.5596709281433334
1101	82-83	0.6136339463652263
1101	84-85	0.5160705143651896
1101	86-87	0.5576100801563868
1101	88-89	0.6464394362981736
1101	90-91	0.6007081213520351
1101	92-93	0.5995287970919669
1101	94-95	0.5762221421597609
1101	96-97	0.600865031511205
1101	98-99	0.6432214689398172
1101	100	0.7123979559610056
1102	1	0.3378607067418784
1102	2	0.37663745334520016
1102	3	0.40036566015210084
1102	4	0.3832340816605182
1102	5	0.3828097253406497
1102	6	0.49731187045565406
1102	7	0.45745058977012576
1102	8	0.49027194499301174
1102	9	0.6351227314464722
1102	10-11	0.5906554718116297
1102	12-13	0.5560797703593394
1102	14-15	0.669841471490976
1102	16-17	0.6172027176066095
1102	18-19	0.5265692838407716
1102	20-21	0.6452352786526987
1102	22-23	0.5868203056145731
1102	24-25	0.6924990763223562
1102	26-27	0.6214901464815412
1102	28-29	0.6219166127882048
1102	30-31	0.6581045608856684
1102	32-33	0.7142122958350896
1102	34-35	0.6938884092070907
1102	36-37	0.6952717650567877
1102	38-39	0.6378784009103811
1102	40-41	0.7173238395090635
1102	42-43	0.7072917891268347
1102	44-45	0.5732827938194092
1102	46-47	0.6044858934603639
1102	48-49	0.5059738530430451
1102	50-51	0.6254198965872959
1102	52-53	0.5536327604278455
1102	54-55	0.5397663099208501
1102	56-57	0.6160462722444393
1102	58-59	0.5509602545927592
1102	60-61	0.5993153829947317
1102	62-63	0.5378961067383159
1102	64-65	0.6341440938521146
1102	66-67	0.60490980669897
1102	68-69	0.5738107175631626
1102	70-71	0.551393181892351
1102	72-73	0.48668618744815717
1102	74-75	0.49452246029909475
1102	76-77	0.4732963528027554
1102	78-79	0.4878942041411065
1102	80-81	0.4109008848971598
1102	82-83	0.4580554449393617
1102	84-85	0.4282092983834289
1102	86-87	0.4468643145887654
1102	88-89	0.413108519289068
1102	90-91	0.3299215415062591
1102	92-93	0.41544615960726006
1102	94-95	0.3724159191051051
1102	96-97	0.311681583824452
1102	98-99	0.35876351752762403
1102	100	0.38890554259112875
1103	1	0.3864618200415393
1103	2	0.3614710679466313
1103	3	0.3947539763601995
1103	4	0.45420429889294667
1103	5	0.4114796513104153
1103	6	0.4265025459852936
1103	7	0.40458424370248736
1103	8	0.3611400917044989
1103	9	0.5965506003154317
1103	10-11	0.61334218626358
1103	12-13	0.6794780058543282
1103	14-15	0.8428305588868952
1103	16-17	0.7664108098254516
1103	18-19	0.7206249690547537
1103	20-21	0.6828874772651758
1103	22-23	0.6624755702552605
1103	24-25	0.7279679382050332
1103	26-27	0.6953618012946166
1103	28-29	0.6372265229282519
1103	30-31	0.7141162125371849
1103	32-33	0.6797101503835492
1103	34-35	0.6630293969292822
1103	36-37	0.6879249969233427
1103	38-39	0.7341078036427575
1103	40-41	0.5539350447738229
1103	42-43	0.6806527764205583
1103	44-45	0.529264905521643
1103	46-47	0.627842517277486
1103	48-49	0.6154294567323007
1103	50-51	0.6793725789420861
1103	52-53	0.5821718059568255
1103	54-55	0.4808463498234019
1103	56-57	0.4982216206390575
1103	58-59	0.5175832371886244
1103	60-61	0.5661663685750256
1103	62-63	0.5675405981485113
1103	64-65	0.5286633334439088
1103	66-67	0.5277093846560703
1103	68-69	0.4978691907261634
1103	70-71	0.4179084207319832
1103	72-73	0.47222697631605826
1103	74-75	0.5627690474472686
1103	76-77	0.4679783985017423
1103	78-79	0.4077249480091396
1103	80-81	0.3854104756946626
1103	82-83	0.41121501472586175
1103	84-85	0.3986687049534723
1103	86-87	0.42167164288191117
1103	88-89	0.45635441338641414
1103	90-91	0.47217419476961453
1103	92-93	0.4213492826016214
1103	94-95	0.45525498354328064
1103	96-97	0.5107044990667475
1103	98-99	0.5047700489530804
1103	100	0.47409029180860074
1104	1	0.2176406562877311
1104	2	0.300319115405447
1104	3	0.2631592155837943
1104	4	0.2723349316789978
1104	5	0.19692351407664432
1104	6	0.31870673636523605
1104	7	0.2834407784497799
1104	8	0.37029646507300384
1104	9	0.42748161721691247
1104	10-11	0.4424044411443617
1104	12-13	0.499633119436524
1104	14-15	0.5593868415857983
1104	16-17	0.5220992845944536
1104	18-19	0.514023317825071
1104	20-21	0.5174242018317017
1104	22-23	0.5595445482497396
1104	24-25	0.49192609620094174
1104	26-27	0.5333858380393224
1104	28-29	0.5379680623904122
1104	30-31	0.5761977291514739
1104	32-33	0.5585832960449935
1104	34-35	0.5012866244770251
1104	36-37	0.5401109203106245
1104	38-39	0.5195964634696537
1104	40-41	0.5572500728341936
1104	42-43	0.6087408706834125
1104	44-45	0.4980884298937056
1104	46-47	0.4947706155798244
1104	48-49	0.5321127798848977
1104	50-51	0.5511614545422461
1104	52-53	0.5590798326239721
1104	54-55	0.519685105196082
1104	56-57	0.5285790346483026
1104	58-59	0.5648412651591244
1104	60-61	0.5125732022640932
1104	62-63	0.5146097948875479
1104	64-65	0.49818043753587204
1104	66-67	0.48243591660161655
1104	68-69	0.5442868294844843
1104	70-71	0.5408242181169243
1104	72-73	0.557857628697974
1104	74-75	0.43231346726472
1104	76-77	0.40855074543569714
1104	78-79	0.3977861071620197
1104	80-81	0.3786463473944224
1104	82-83	0.4141648077479516
1104	84-85	0.37087479476524976
1104	86-87	0.34043212780940024
1104	88-89	0.30277279496477405
1104	90-91	0.3316015880765022
1104	92-93	0.346139825582096
1104	94-95	0.40625171586429687
1104	96-97	0.3362478833110458
1104	98-99	0.45315169019707113
1104	100	0.44302765448943404
1105	1	0.09040378171788532
1105	2	0.12453587492081652
1105	3	0.13920455345351357
1105	4	0.14142349938496324
1105	5	0.1230658128287061
1105	6	0.14446582320150014
1105	7	0.14403734759284958
1105	8	0.2524596450436576
1105	9	0.18580518953285008
1105	10-11	0.32367618700295964
1105	12-13	0.28218539823368616
1105	14-15	0.34934126131387444
1105	16-17	0.31067034523396586
1105	18-19	0.27767841844362806
1105	20-21	0.34994256596309725
1105	22-23	0.3325296448426869
1105	24-25	0.36875930616886876
1105	26-27	0.3213773193332372
1105	28-29	0.3109872900796731
1105	30-31	0.3053947718445613
1105	32-33	0.24809665197367536
1105	34-35	0.24021862632967128
1105	36-37	0.30047590877487096
1105	38-39	0.22888103067349164
1105	40-41	0.1962392123496457
1105	42-43	0.24143019329910942
1105	44-45	0.1835633434297037
1105	46-47	0.1259768479136838
1105	48-49	0.10471018444984992
1105	50-51	0.1651466550218501
1105	52-53	0.17805141962256243
1105	54-55	0.1446242449279751
1105	56-57	0.1773193223277758
1105	58-59	0.11919545806799903
1105	60-61	0.18173347340827206
1105	62-63	0.11588361847933726
1105	64-65	0.19749294592255495
1105	66-67	0.15545897511144346
1105	68-69	0.13944627386399588
1105	70-71	0.1436691110054511
1105	72-73	0.17360485514059576
1105	74-75	0.17017169227456463
1105	76-77	0.06685509871959994
1105	78-79	0.16891479243305696
1105	80-81	0.15626292770529915
1105	82-83	0.12098265114746454
1105	84-85	0.13612838643008018
1105	86-87	0.038213130402240836
1105	88-89	0.08296085146179877
1105	90-91	0.10332807614469175
1105	92-93	0.1550443191129851
1105	94-95	0.14843998013464343
1105	96-97	0.12585255128495376
1105	98-99	0.09277966774024193
1105	100	0.2418222229485778
1106	1	0.13909229088329766
1106	2	0.14953126023395313
1106	3	0.19906760350251318
1106	4	0.22237168274185848
1106	5	0.1847138427855839
1106	6	0.24968330551715212
1106	7	0.19803495620185174
1106	8	0.15184915315926872
1106	9	0.24519101858539472
1106	10-11	0.2585031283166188
1106	12-13	0.2904235604782528
1106	14-15	0.35874879472410015
1106	16-17	0.38553949005267185
1106	18-19	0.35499538769913386
1106	20-21	0.3845270274191037
1106	22-23	0.32017695801658874
1106	24-25	0.2834324457311652
1106	26-27	0.278430453250067
1106	28-29	0.32336987457917843
1106	30-31	0.33500843850664097
1106	32-33	0.3263026224164278
1106	34-35	0.2778915132206805
1106	36-37	0.317290981938541
1106	38-39	0.19420053298506446
1106	40-41	0.19165647413295517
1106	42-43	0.22060737295895905
1106	44-45	0.3164628362898938
1106	46-47	0.23656582573675422
1106	48-49	0.27243560370973796
1106	50-51	0.22962244668116227
1106	52-53	0.22038603886596064
1106	54-55	0.1841878953620082
1106	56-57	0.2519196841023046
1106	58-59	0.1477132925376452
1106	60-61	0.23334121339577507
1106	62-63	0.19727808910128175
1106	64-65	0.26311347594022294
1106	66-67	0.2328729320812073
1106	68-69	0.1853160075442375
1106	70-71	0.17588825207108982
1106	72-73	0.19636924815333145
1106	74-75	0.2262896041314093
1106	76-77	0.23681244061767615
1106	78-79	0.26527943409971755
1106	80-81	0.20623255429097753
1106	82-83	0.27554863271197405
1106	84-85	0.24701915314470568
1106	86-87	0.12594554833957972
1106	88-89	0.18975136582230334
1106	90-91	0.20220479898755173
1106	92-93	0.22243893985762142
1106	94-95	0.14663893075590906
1106	96-97	0.13186160304873695
1106	98-99	0.14920469678686388
1106	100	0.17180208324990076
1107	1	0.046051617184531324
1107	2	0.08130422630846112
1107	3	0.06331533946868007
1107	4	0.02311277309834736
1107	5	-6.168907443537819E-4
1107	6	0.09424973533788261
1107	7	0.056373715387046275
1107	8	0.08447611007426303
1107	9	0.1787564689977188
1107	10-11	0.10258774907157431
1107	12-13	0.18667771601462135
1107	14-15	0.20352136168289547
1107	16-17	0.1775664238250343
1107	18-19	0.18998026480760188
1107	20-21	0.2661512441452629
1107	22-23	0.20884211704072442
1107	24-25	0.27387605877363796
1107	26-27	0.16608760454749216
1107	28-29	0.2179728153045417
1107	30-31	0.2507152906893353
1107	32-33	0.29470450899271583
1107	34-35	0.24084435596818565
1107	36-37	0.2533990782306148
1107	38-39	0.24765325397473248
1107	40-41	0.15794417002800287
1107	42-43	0.22148595386698844
1107	44-45	0.21856657002435753
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2216	80-81	-0.6489593861040746
2216	82-83	-0.6846967691578101
2216	84-85	-0.7079353447816956
2216	86-87	-0.6890716181360048
2216	88-89	-0.6477618506506246
2216	90-91	-0.608876284035496
2216	92-93	-0.6017857343508872
2216	94-95	-0.5919577485446759
2216	96-97	-0.6700289424760655
2216	98-99	-0.6410511641635068
2216	100	-0.7220298899793001
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1000.0
1	248.0
2	175.0
3	195.0
4	142.0
5	185.0
6	267.0
7	605.0
8	1516.0
9	2140.0
10	2758.0
11	4291.0
12	6758.0
13	9142.0
14	10860.0
15	11702.0
16	12694.0
17	13870.0
18	15268.0
19	16603.0
20	17990.0
21	19569.0
22	21613.0
23	24542.0
24	29347.0
25	35482.0
26	38498.0
27	36622.0
28	39121.0
29	45855.0
30	53381.0
31	61648.0
32	78057.0
33	102890.0
34	151588.0
35	229269.0
36	412722.0
37	763828.0
38	819894.0
39	221697.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63697144698546	20.5463739214814	13.599549882985883	24.217104748547253
2	14.63595191291938	20.910495341371597	40.702352274487275	23.75120047122175
3	17.393272323802446	26.75809540834182	30.73379023547561	25.114842032380125
4	11.959007695870875	18.0179584412102	40.21157454631322	29.8114593166057
5	12.86796279274263	39.03675913724192	35.45409305196382	12.641185018051631
6	29.224769822114954	39.20579871907305	18.800508070419575	12.768923388392414
7	26.167012988503284	33.20404521621384	23.52652162923562	17.102420166047253
8	24.986810710518427	36.07662501559009	21.064752390152925	17.871811883738566
9	26.223342213349248	15.828801988930325	20.983559138158373	36.96429665956205
10-11	24.08117188217162	26.890635644195786	29.202992815696277	19.825199657936313
12-13	24.51822523915503	24.864408616857688	28.56191762432846	22.055448519658828
14-15	22.349108955288102	26.00290937969217	25.877248937668767	25.770732727350964
16-17	21.9009959589417	28.74612445674452	26.821001424524006	22.531878159789773
18-19	21.873677487832698	28.710602065289354	27.924013537089227	21.49170690978872
20-21	23.138573311811086	27.836494941607924	27.15530438216842	21.869627364412565
22-23	23.201154657354525	27.550716301231503	26.851611130486248	22.396517910927727
24-25	22.728092133671122	27.83753710318838	27.213448641336345	22.220922121804144
26-27	22.532538243409657	28.019537363705933	26.917469539107415	22.530454853776995
28-29	22.289653052399668	28.16186257860192	27.08113904235505	22.46734532664336
30-31	22.87128630649008	27.82117649900988	26.90735479857556	22.400182395924478
32-33	22.803895970944197	28.119967228570452	26.55393258664483	22.522204213840524
34-35	22.89005266591863	27.989975069039176	27.097603405504923	22.022368859537266
36-37	22.851829957441797	27.793398306882622	26.950327623422186	22.404444112253397
38-39	22.709293590484066	27.98181976044061	27.086322163206912	22.22256448586841
40-41	22.65447005135727	27.49920313413272	27.427556927780472	22.418769886729535
42-43	22.505192953167082	27.57760135394287	27.198888747282723	22.718316945607324
44-45	22.478860045277955	27.94469403406401	27.15345475805829	22.42299116259974
46-47	22.544514885253385	27.781347044956306	26.995678779241167	22.678459290549135
48-49	22.744606173705087	27.54478955556928	27.17082283400181	22.539781436723818
50-51	22.54025212200574	27.63338300233084	26.958170529956327	22.868194345707092
52-53	22.37574258472337	27.79708674235217	26.920330890014405	22.90683978291006
54-55	22.47421696599966	27.68377824710853	26.96879460531698	22.87321018157483
56-57	22.708329149236285	27.50616591191848	26.929553295480407	22.855951643364833
58-59	22.43023128858453	27.492412775802794	27.294451046189007	22.782904889423673
60-61	22.520366221255227	27.735148744729216	27.060334331151203	22.684150702864354
62-63	22.57456524966177	27.475729079001432	26.960735086987818	22.988970584348973
64-65	22.227640260450414	27.533176591114984	27.06388622139856	23.17529692703604
66-67	22.474217961052958	27.342228147467118	26.976419112680144	23.20713477879978
68-69	22.199327027246607	27.31759862107333	27.139615117090678	23.343459234589385
70-71	22.339099173436402	27.056358970285448	27.166614767964475	23.43792708831367
72-73	22.454933745372262	27.205547907680245	27.269078294443936	23.070440052503553
74-75	22.240315709625552	27.488737546590563	27.23155598169133	23.039390762092555
76-77	22.239662528562192	27.21182104433541	27.219009256030663	23.329507171071736
78-79	22.126596440187758	27.27490886168436	27.202191518001356	23.396303180126523
80-81	22.081713369693006	27.245260166978643	27.418536184620358	23.254490278707994
82-83	22.413559281079014	26.82893383613301	27.807701775555415	22.949805107232553
84-85	22.400813795255168	27.05700085459979	27.265478502639684	23.27670684750536
86-87	21.891050635365808	27.371454476373646	27.548455061694238	23.189039826566308
88-89	22.348340874025663	26.714271109445974	27.361002320875144	23.57638569565322
90-91	22.20030881792201	26.740460810703677	27.616869462135828	23.442360909238488
92-93	22.37633937894524	26.674583257768848	27.561107571765376	23.38796979152054
94-95	21.82790917155854	26.649925442156324	27.8141150267087	23.708050359576436
96-97	21.96574386098587	26.677197399600544	27.624631163872486	23.732427575541095
98-99	21.833877933869584	26.887902800395008	27.514949771757074	23.763269493978335
100	22.293103636013875	26.48117991088118	27.230722883549074	23.99499356955587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1290.0
1	881.0
2	454.0
3	444.5
4	425.5
5	391.5
6	358.0
7	333.0
8	364.5
9	374.5
10	341.0
11	348.0
12	380.5
13	400.5
14	420.5
15	501.0
16	591.5
17	709.5
18	896.5
19	1231.0
20	1716.5
21	2446.5
22	3729.5
23	5434.5
24	7502.0
25	10409.0
26	14520.0
27	19306.0
28	23748.5
29	29915.0
30	37892.5
31	45499.5
32	54583.0
33	63165.5
34	70560.5
35	78860.5
36	88578.0
37	99938.0
38	105375.0
39	106529.5
40	106812.0
41	105178.0
42	106020.0
43	111227.5
44	117455.0
45	125926.5
46	131983.0
47	135822.0
48	139875.0
49	137557.5
50	131921.0
51	128313.5
52	123420.5
53	116504.0
54	110786.5
55	100425.0
56	90153.0
57	79826.5
58	69639.5
59	61880.5
60	49829.0
61	37858.5
62	29352.0
63	22661.0
64	17009.5
65	12079.0
66	8758.5
67	6438.5
68	4720.5
69	3447.5
70	2676.0
71	2088.0
72	1522.5
73	1255.0
74	1056.5
75	794.5
76	562.5
77	391.5
78	250.5
79	145.0
80	95.5
81	80.5
82	66.0
83	48.5
84	33.5
85	26.0
86	22.5
87	21.5
88	16.0
89	6.5
90	6.0
91	6.0
92	6.5
93	7.0
94	4.5
95	2.0
96	1.5
97	4.0
98	6.0
99	5.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09537458890263403
2	0.06212473222098181
3	0.07775397519838277
4	0.0800772410463748
5	0.08110309869354011
6	0.08894789246598074
7	0.08547807983586278
8	0.0875297951301934
9	0.07147814018043025
10-11	0.07953413993905199
12-13	0.0702109042633437
14-15	0.07837250701505596
16-17	0.08048456687686692
18-19	0.08018284403946535
20-21	0.07174969073409167
22-23	0.07527984793168994
24-25	0.0781462148870048
26-27	0.07209667199710346
28-29	0.08074103128865824
30-31	0.08516127085659113
32-33	0.08686600488790996
34-35	0.08917418459403192
36-37	0.08908366774281146
38-39	0.09089400476722083
40-41	0.0871828138671816
42-43	0.0929909784871617
44-45	0.08977763026883505
46-47	0.08603626708505567
48-49	0.09584225930060646
50-51	0.09190477627251607
52-53	0.09745647648070482
54-55	0.08947590743143348
56-57	0.09508795220710256
58-59	0.09976465618682678
60-61	0.09780345774371663
62-63	0.09928189964698428
64-65	0.10088103068521256
66-67	0.10317412424946444
68-69	0.10014180973357875
70-71	0.10146939021814562
72-73	0.09914612437015359
74-75	0.10287240141206289
76-77	0.10027758501040944
78-79	0.10667410916332257
80-81	0.102510334007181
82-83	0.1057538545092478
84-85	0.10238964487222038
86-87	0.09938750264007483
88-89	0.09849742026974023
90-91	0.09919138279576381
92-93	0.09385088857375615
94-95	0.09391123314123646
96-97	0.09170865642820504
98-99	0.10450170473403132
100	0.24596445704975411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3314300.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.08634528290462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.20675003721229	39.37640023777665
2	15.519514878603239	17.408657402090334
3	6.112276594840984	10.28445766688604
4	2.9696196723160964	6.662204572017067
5	1.636110068612074	4.588171711450678
6	0.9890029751279249	3.3281737409306835
7	0.6514656846114771	2.5576830528958205
8	0.44290225803044014	1.9872615176378707
9	0.307588223805661	1.5526349392787682
>10	1.1229897323242586	9.884334653652637
>50	0.028457287453788734	1.0893620251597247
>100	0.01305460494850171	1.1835136925673388
>500	2.679821133189639E-4	0.09714478765642375
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.034456748031258E-5
2	0.0	0.0	0.0	0.0	6.034456748031258E-5
3	0.0	0.0	0.0	0.0	6.034456748031258E-5
4	0.0	0.0	0.0	0.0	6.034456748031258E-5
5	0.0	0.0	0.0	0.0	6.034456748031258E-5
6	0.0	0.0	0.0	0.0	6.034456748031258E-5
7	3.017228374015629E-5	0.0	0.0	0.0	6.034456748031258E-5
8	3.017228374015629E-5	0.0	0.0	0.0	9.051685122046888E-5
9	3.017228374015629E-5	0.0	0.0	0.0	1.2068913496062516E-4
10-11	3.017228374015629E-5	0.0	0.0	0.0	1.2068913496062516E-4
12-13	3.017228374015629E-5	0.0	0.0	1.5086141870078145E-5	1.961198443110159E-4
14-15	3.017228374015629E-5	0.0	0.0	3.017228374015629E-5	2.2629212805117219E-4
16-17	3.017228374015629E-5	0.0	0.0	3.017228374015629E-5	2.4137826992125033E-4
18-19	3.017228374015629E-5	0.0	0.0	9.051685122046888E-5	3.0172283740156295E-4
20-21	3.017228374015629E-5	0.0	0.0	9.051685122046888E-5	3.620674048818755E-4
22-23	3.017228374015629E-5	0.0	0.0	1.5086141870078148E-4	3.771535467519537E-4
24-25	3.017228374015629E-5	3.017228374015629E-5	0.0	3.771535467519537E-4	3.922396886220318E-4
26-27	6.034456748031258E-5	3.017228374015629E-5	0.0	7.392209516338291E-4	3.922396886220318E-4
28-29	6.034456748031258E-5	3.017228374015629E-5	0.0	0.0031530036508463327	3.922396886220318E-4
30-31	6.034456748031258E-5	3.017228374015629E-5	0.0	0.011737018374920798	3.922396886220318E-4
32-33	6.034456748031258E-5	3.017228374015629E-5	0.0	0.0325106357300184	4.5258425610234437E-4
34-35	6.034456748031258E-5	3.017228374015629E-5	0.0	0.06256223033521408	4.5258425610234437E-4
36-37	6.034456748031258E-5	3.017228374015629E-5	0.0	0.11138098542678695	4.5258425610234437E-4
38-39	6.034456748031258E-5	3.017228374015629E-5	0.0	0.18503153003650846	4.5258425610234437E-4
40-41	6.034456748031258E-5	3.017228374015629E-5	0.0	0.270087197900009	4.8275653984250066E-4
42-43	6.034456748031258E-5	3.017228374015629E-5	0.0	0.3583713001237063	4.8275653984250066E-4
44-45	6.034456748031258E-5	3.017228374015629E-5	0.0	0.4686208249102375	4.978426817125788E-4
46-47	7.543070935039074E-5	3.017228374015629E-5	0.0	0.6009112029689527	5.12928823582657E-4
48-49	9.051685122046888E-5	3.017228374015629E-5	0.0	0.7462209214615454	5.12928823582657E-4
50-51	9.051685122046888E-5	3.017228374015629E-5	0.0	0.9024831789518148	5.431011073228132E-4
52-53	9.051685122046888E-5	3.017228374015629E-5	0.0	1.0599674139335606	5.732733910629696E-4
54-55	9.051685122046888E-5	3.017228374015629E-5	0.0	1.2374709591769002	6.034456748031259E-4
56-57	9.051685122046888E-5	3.017228374015629E-5	0.0	1.4512868479015175	6.336179585432822E-4
58-59	9.051685122046888E-5	3.017228374015629E-5	0.0	1.6948526083939293	6.637902422834385E-4
60-61	9.051685122046888E-5	3.017228374015629E-5	0.0	1.9506381438011045	6.637902422834385E-4
62-63	9.051685122046888E-5	3.017228374015629E-5	0.0	2.2088374619074918	7.24134809763751E-4
64-65	9.051685122046888E-5	3.017228374015629E-5	0.0	2.4844461877319493	7.24134809763751E-4
66-67	9.051685122046888E-5	3.017228374015629E-5	0.0	2.800199137072685	7.24134809763751E-4
68-69	9.051685122046888E-5	3.017228374015629E-5	0.0	3.14865884198775	7.24134809763751E-4
70-71	9.051685122046888E-5	3.017228374015629E-5	0.0	3.525239115348641	7.543070935039073E-4
72-73	9.051685122046888E-5	3.017228374015629E-5	0.0	3.91123314123646	7.693932353739854E-4
74-75	9.051685122046888E-5	3.017228374015629E-5	0.0	4.3026581781975075	7.844793772440636E-4
76-77	9.051685122046888E-5	3.017228374015629E-5	0.0	4.741634734333042	7.844793772440636E-4
78-79	9.051685122046888E-5	3.017228374015629E-5	0.0	5.2278912590894	7.844793772440636E-4
80-81	9.051685122046888E-5	3.017228374015629E-5	0.0	5.746341610596506	7.844793772440636E-4
82-83	9.051685122046888E-5	3.017228374015629E-5	0.0	6.289895302175422	7.844793772440636E-4
84-85	9.051685122046888E-5	3.017228374015629E-5	0.0	6.826479196210361	7.844793772440636E-4
86-87	9.051685122046888E-5	3.017228374015629E-5	0.0	7.384726789970733	7.844793772440636E-4
88	9.051685122046888E-5	3.017228374015629E-5	0.0	7.8273541924388255	7.844793772440636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3130	0.0	38.915203	1
GTATCAA	5340	0.0	36.108253	1
ATCAACG	5375	0.0	33.237717	3
CAACGCA	5415	0.0	32.904877	5
AACGCAG	6150	0.0	30.260006	6
TATCAAC	6425	0.0	29.26934	2
TCAACGC	6285	0.0	28.649233	4
ACGCAGA	6515	0.0	27.266306	7
CGCAGAG	6790	0.0	25.884373	8
GTACATG	11430	0.0	25.3865	1
TACATGG	11205	0.0	24.797144	2
GTACTAG	1625	0.0	23.731455	1
ACATGGG	11415	0.0	23.352491	3
TAACGCC	645	0.0	23.324362	4
TAGTACT	1995	0.0	21.444605	4
GCAGAGT	8155	0.0	21.37763	9
CTAACGC	595	0.0	21.334026	3
CATGGGG	8665	0.0	20.942968	4
CTAGTAC	2095	0.0	20.645712	3
CTGTGCG	1915	0.0	20.611998	9
>>END_MODULE