FastQCFastQC Report
Mon 16 Jan 2017
ERR1146603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314300
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.26298162507920225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83290.2513049512717618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.132818393024168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.063.2699851
GTATCAA112500.043.0529821
ATCAACG137550.034.4219133
CAACGCA139200.034.0680775
TATCAAC144500.033.2899552
AACGCAG147100.032.684786
TCAACGC147000.032.4009864
ACGCAGA159900.029.5971897
CGCAGAG164000.028.7712928
CTAACGC5550.026.2624243
GCAGAGT182450.025.6557669
GTGGTAT17050.024.5461941
TACATGG106550.024.316642
GTACATG108800.024.2466931
TGATCGC9300.023.7511379
TAACGCC6350.023.6942184
ACATGGG107600.023.59653
GGCGTCG10350.023.1579078
GAGTACT122450.023.14344612-13
CAGAGTA178750.022.7162810-11
Produced by FastQC (version 0.11.5)