FastQCFastQC Report
Mon 16 Jan 2017
ERR1146602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372467
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.043.5221861
GTATCAA44900.036.4453541
ATCAACG48700.031.7589863
CAACGCA49450.031.7512265
TATCAAC57450.028.3948212
AACGCAG56750.028.321626
GTACATG108850.027.6478161
TCAACGC57100.027.3330944
TACATGG110600.026.480892
ACATGGG107550.025.8768253
ACGCAGA62600.024.8487837
CGCAGAG65900.023.5320978
TGATCGC7700.023.1906079
CATGGGG80400.023.0955244
GTGATCG8150.022.4873928
GTATAAG15350.020.5246471
GTACTAG12450.020.395471
ATGGGGG52350.020.2942075
ACGGGCG6900.019.7573765
GTATAAT12050.019.511571
Produced by FastQC (version 0.11.5)