##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146602_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372467 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53396697432473 31.0 31.0 34.0 27.0 34.0 2 30.799345405010634 31.0 31.0 34.0 27.0 34.0 3 30.955766505647052 31.0 31.0 34.0 27.0 34.0 4 34.51933169397951 37.0 35.0 37.0 30.0 37.0 5 34.544850698316694 37.0 35.0 37.0 30.0 37.0 6 34.603038072722434 37.0 35.0 37.0 31.0 37.0 7 34.484294434904776 37.0 35.0 37.0 30.0 37.0 8 34.48075755819108 37.0 35.0 37.0 30.0 37.0 9 36.09318341736183 39.0 37.0 39.0 30.0 39.0 10-11 36.0667406085812 39.0 37.0 39.0 30.0 39.0 12-13 36.01185141322361 39.0 37.0 39.0 30.0 39.0 14-15 37.254739334736264 40.0 38.0 41.0 30.5 41.0 16-17 37.257312228703796 40.0 37.0 41.0 30.5 41.0 18-19 37.24871347888653 40.0 37.0 41.0 30.0 41.0 20-21 37.33925506165072 40.0 37.5 41.0 30.0 41.0 22-23 37.33668646720635 40.0 37.0 41.0 30.0 41.0 24-25 37.344653780155596 40.0 37.0 41.0 30.0 41.0 26-27 37.39714680677379 40.0 37.0 41.0 30.0 41.0 28-29 37.36037847071594 40.0 37.0 41.0 30.0 41.0 30-31 37.31896857700906 40.0 37.0 41.0 30.0 41.0 32-33 37.285881670599004 40.0 37.0 41.0 30.0 41.0 34-35 37.23939329873354 40.0 37.0 41.0 30.0 41.0 36-37 37.15562079628948 40.0 37.0 41.0 30.0 41.0 38-39 37.09808724592413 40.0 37.0 41.0 30.0 41.0 40-41 37.04296216982998 40.0 37.0 41.0 30.0 41.0 42-43 36.882994407358176 40.0 36.0 41.0 29.5 41.0 44-45 36.75329854969671 40.0 36.0 41.0 29.0 41.0 46-47 36.629970582365964 40.0 35.5 41.0 29.0 41.0 48-49 36.52067640691517 39.0 35.0 41.0 29.0 41.0 50-51 36.39519956755693 39.0 35.0 41.0 28.0 41.0 52-53 36.25994842944349 39.0 35.0 41.0 28.0 41.0 54-55 36.13591311642189 39.0 35.0 41.0 28.0 41.0 56-57 35.94695885830758 39.0 35.0 40.0 28.0 41.0 58-59 35.75569620103028 38.0 34.5 40.0 28.0 41.0 60-61 35.532458286471 38.0 34.0 40.0 28.0 41.0 62-63 35.29343652584295 37.0 34.0 40.0 27.0 41.0 64-65 34.99758781924331 37.0 34.0 40.0 27.0 41.0 66-67 34.75353116872604 36.5 34.0 39.5 26.0 41.0 68-69 34.5017696244322 36.0 33.5 39.0 26.0 41.0 70-71 34.21307754827549 35.5 33.0 39.0 26.0 40.5 72-73 33.88137289408614 35.0 33.0 38.5 26.0 40.0 74-75 33.558668327962884 35.0 33.0 37.0 26.0 39.5 76-77 33.30048537168785 35.0 33.0 37.0 26.0 39.0 78-79 32.994482673959446 35.0 33.0 36.0 25.5 39.0 80-81 32.76885674492886 35.0 32.0 36.0 25.0 38.5 82-83 32.52545851449399 35.0 32.0 35.5 25.0 37.0 84-85 32.33998227410379 35.0 32.0 35.0 25.0 37.0 86-87 32.15060132537991 35.0 32.0 35.0 25.0 36.5 88-89 31.977613717198714 35.0 32.0 35.0 24.5 36.0 90-91 31.846099606015418 35.0 32.0 35.0 24.0 36.0 92-93 31.71705134549871 35.0 32.0 35.0 24.0 36.0 94-95 31.603770325995775 35.0 32.0 35.0 24.0 35.0 96-97 31.523152932259976 35.0 31.5 35.0 23.5 35.0 98-99 31.42414366100543 35.0 31.0 35.0 23.0 35.0 100 30.19837525467262 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4297098912721964 1101 2 0.3936112916150023 1101 3 0.4699656183165146 1101 4 0.47832214440866494 1101 5 0.4273585311458703 1101 6 0.5991213715487902 1101 7 0.6567437959394624 1101 8 0.6514323720825885 1101 9 0.7847338154107462 1101 10-11 0.7551565241889406 1101 12-13 0.8058131420526209 1101 14-15 0.8864915025102178 1101 16-17 0.9484226016354498 1101 18-19 0.8536453481819564 1101 20-21 0.8683682663312595 1101 22-23 0.9051821081248335 1101 24-25 0.9668472710363076 1101 26-27 0.9228862402942255 1101 28-29 0.8952376088121667 1101 30-31 0.9025171340509033 1101 32-33 0.8952679308427065 1101 34-35 0.8573045234107752 1101 36-37 0.894856322364177 1101 38-39 0.9382071957901417 1101 40-41 0.8837176444070991 1101 42-43 0.9113394878520893 1101 44-45 0.8482774032576614 1101 46-47 0.848622721430111 1101 48-49 0.8745647244161177 1101 50-51 0.7889345327207167 1101 52-53 0.7068950993489054 1101 54-55 0.7594921698128161 1101 56-57 0.801331965000486 1101 58-59 0.7659153339964462 1101 60-61 0.7399701436095754 1101 62-63 0.7281273535269435 1101 64-65 0.7447374146397578 1101 66-67 0.6644095464023252 1101 68-69 0.7062719340878516 1101 70-71 0.6445241110056585 1101 72-73 0.7057413087504898 1101 74-75 0.6924675679424581 1101 76-77 0.6192631155474402 1101 78-79 0.6306475756614986 1101 80-81 0.6221920477897029 1101 82-83 0.6940018229326412 1101 84-85 0.5961503434551574 1101 86-87 0.6119633599194856 1101 88-89 0.6843146885744815 1101 90-91 0.5744607892489846 1101 92-93 0.5679905277499557 1101 94-95 0.5864273272764642 1101 96-97 0.6403328742806771 1101 98-99 0.6538383406372006 1101 100 0.6284425885289302 1102 1 0.2431620769891083 1102 2 0.347716822976853 1102 3 0.30856387594159784 1102 4 0.3142934835356428 1102 5 0.3528762200911473 1102 6 0.46967946491066925 1102 7 0.5044224629982494 1102 8 0.5050412867819034 1102 9 0.5770903879723832 1102 10-11 0.5956762657821812 1102 12-13 0.6303782439551355 1102 14-15 0.7271524465912833 1102 16-17 0.6944018302935859 1102 18-19 0.6888576142411011 1102 20-21 0.7094905532079707 1102 22-23 0.7144029495007373 1102 24-25 0.8053602549443184 1102 26-27 0.7097267548988455 1102 28-29 0.671976591186791 1102 30-31 0.7040985933530308 1102 32-33 0.6717421023670198 1102 34-35 0.6509109423983972 1102 36-37 0.6791567332790436 1102 38-39 0.6288000321985905 1102 40-41 0.681392822606476 1102 42-43 0.7049703502596145 1102 44-45 0.5803103980218012 1102 46-47 0.5808919954519851 1102 48-49 0.5840241994947419 1102 50-51 0.5972969006417017 1102 52-53 0.5355208911380558 1102 54-55 0.5692092639824793 1102 56-57 0.5487510652001077 1102 58-59 0.5575698324632796 1102 60-61 0.6233104974698094 1102 62-63 0.5355475367712614 1102 64-65 0.5571510760714204 1102 66-67 0.6117699567093098 1102 68-69 0.480221883724127 1102 70-71 0.5149111004282005 1102 72-73 0.5524358392035253 1102 74-75 0.634162391630916 1102 76-77 0.592242926823694 1102 78-79 0.5702065752706602 1102 80-81 0.5190430632190228 1102 82-83 0.5329031832453524 1102 84-85 0.5281765103287981 1102 86-87 0.5027865894731676 1102 88-89 0.3626696763805981 1102 90-91 0.4587140470517248 1102 92-93 0.4282718967698287 1102 94-95 0.44406833605768625 1102 96-97 0.439797687528241 1102 98-99 0.4897472655676509 1102 100 0.48537238368793467 1103 1 0.26231500606315805 1103 2 0.30933308891998124 1103 3 0.3679809220897887 1103 4 0.4782272291086471 1103 5 0.3756163985860397 1103 6 0.4011767583613448 1103 7 0.4498771023470667 1103 8 0.4471142112701614 1103 9 0.6297202491487681 1103 10-11 0.5842728159000785 1103 12-13 0.591059172723476 1103 14-15 0.6856485119987852 1103 16-17 0.6365581047266318 1103 18-19 0.636225584107045 1103 20-21 0.6056702957630193 1103 22-23 0.583834796202936 1103 24-25 0.6867183736110931 1103 26-27 0.6607784411601543 1103 28-29 0.6757123625662587 1103 30-31 0.7045813413604307 1103 32-33 0.7506737771018663 1103 34-35 0.6896220857799236 1103 36-37 0.6302950745634988 1103 38-39 0.6116036062276962 1103 40-41 0.5296265164343481 1103 42-43 0.6164337046869193 1103 44-45 0.5644858894198208 1103 46-47 0.5673043019112427 1103 48-49 0.5366531311178377 1103 50-51 0.5329620011832148 1103 52-53 0.5520868734125131 1103 54-55 0.4332726305972656 1103 56-57 0.47379798182020494 1103 58-59 0.4970659319543955 1103 60-61 0.5116489416248342 1103 62-63 0.4957433420071311 1103 64-65 0.42584475417951495 1103 66-67 0.44793216169233574 1103 68-69 0.4137312469078296 1103 70-71 0.39486239183592176 1103 72-73 0.5269071041293145 1103 74-75 0.5330914429598153 1103 76-77 0.5028368390759255 1103 78-79 0.43341619883256044 1103 80-81 0.37822699805940374 1103 82-83 0.4233832148914729 1103 84-85 0.5114824562680482 1103 86-87 0.41847373075597005 1103 88-89 0.39942875785801135 1103 90-91 0.43683546117081207 1103 92-93 0.30130410992259726 1103 94-95 0.33910865284944336 1103 96-97 0.40079197903821395 1103 98-99 0.36754189006427396 1103 100 0.34685324470216017 1104 1 0.09502602483680178 1104 2 0.07267224173908104 1104 3 0.12977645257818082 1104 4 0.16660805386912614 1104 5 0.08360711291739875 1104 6 0.25595141644789976 1104 7 0.20384079087255458 1104 8 0.18575329012792707 1104 9 0.3293522742036288 1104 10-11 0.35867315717241866 1104 12-13 0.3683189520443335 1104 14-15 0.4601738385663765 1104 16-17 0.4255363799580465 1104 18-19 0.39697063567848545 1104 20-21 0.447461381244338 1104 22-23 0.3971020229459299 1104 24-25 0.49235948243683936 1104 26-27 0.3750386914401034 1104 28-29 0.3288667097384135 1104 30-31 0.4961075174454024 1104 32-33 0.4479011835827009 1104 34-35 0.3726950754827243 1104 36-37 0.4626091769997416 1104 38-39 0.4198600644118642 1104 40-41 0.45484366846295643 1104 42-43 0.45729262089487577 1104 44-45 0.36803597247875786 1104 46-47 0.36417047015638815 1104 48-49 0.31646577732580994 1104 50-51 0.3508686226562716 1104 52-53 0.40606680331002565 1104 54-55 0.28084898234549627 1104 56-57 0.3410286121523285 1104 58-59 0.3728678547862998 1104 60-61 0.3840516959010145 1104 62-63 0.27067323748839556 1104 64-65 0.3525640702997066 1104 66-67 0.33031692834371285 1104 68-69 0.37293737759836176 1104 70-71 0.3906633662368435 1104 72-73 0.3429111852319693 1104 74-75 0.29069763568506346 1104 76-77 0.25274968925761243 1104 78-79 0.25091558520178125 1104 80-81 0.25953236475416475 1104 82-83 0.29150107596384345 1104 84-85 0.2764851896217593 1104 86-87 0.30245314369754084 1104 88-89 0.2719312195045589 1104 90-91 0.2683498083266862 1104 92-93 0.1779530671166043 1104 94-95 0.1756680830418702 1104 96-97 0.18158887681404323 1104 98-99 0.2334313046381169 1104 100 0.2809683891099404 1105 1 0.13000565718062518 1105 2 0.1433778694801262 1105 3 0.13734698734793938 1105 4 0.203957750761397 1105 5 0.1403529668580532 1105 6 0.13097957812297523 1105 7 0.18481537677006799 1105 8 0.2288908405593446 1105 9 0.3394950397367609 1105 10-11 0.37675216593179783 1105 12-13 0.3281977294059999 1105 14-15 0.3996829931560413 1105 16-17 0.4444098940757044 1105 18-19 0.3974967791526254 1105 20-21 0.4063290915396749 1105 22-23 0.31741848465642875 1105 24-25 0.38609895327424226 1105 26-27 0.3582434381455002 1105 28-29 0.33800375735020083 1105 30-31 0.3525866142454177 1105 32-33 0.29027025853932287 1105 34-35 0.4021108890656535 1105 36-37 0.3344425224997636 1105 38-39 0.3440883243853463 1105 40-41 0.40808521885913507 1105 42-43 0.36492445057305645 1105 44-45 0.3532746922332777 1105 46-47 0.33168326405257176 1105 48-49 0.30344462114272375 1105 50-51 0.29835579100141274 1105 52-53 0.22763427270505332 1105 54-55 0.14749224125858262 1105 56-57 0.22325829625700067 1105 58-59 0.18200395056494756 1105 60-61 0.19050385818274407 1105 62-63 0.21078617681840228 1105 64-65 0.2353329167534426 1105 66-67 0.19457903109890395 1105 68-69 0.20021144072708807 1105 70-71 0.1847644314089294 1105 72-73 0.2433296535640892 1105 74-75 0.1905335978609486 1105 76-77 0.1836577333647753 1105 78-79 0.1446758626399003 1105 80-81 0.16973618920404476 1105 82-83 0.16829386550518421 1105 84-85 0.19013264415246312 1105 86-87 0.1973802427177631 1105 88-89 0.1877820104543062 1105 90-91 0.20085177964393708 1105 92-93 0.3028067522684452 1105 94-95 0.28827442876403353 1105 96-97 0.2612669086307591 1105 98-99 0.22614765710019924 1105 100 0.36682979653921066 1106 1 0.19421916896963154 1106 2 0.22091205499627264 1106 3 0.22344388523559644 1106 4 0.2360732839150117 1106 5 0.2396597160360443 1106 6 0.23123396598929702 1106 7 0.2874214594869642 1106 8 0.3517942968150152 1106 9 0.5399103949028259 1106 10-11 0.47937511625098495 1106 12-13 0.506640895508383 1106 14-15 0.5559986890323998 1106 16-17 0.5221037493837315 1106 18-19 0.5099349567188654 1106 20-21 0.45570495209268813 1106 22-23 0.517639824234081 1106 24-25 0.51816884468046 1106 26-27 0.4711084958006424 1106 28-29 0.4595078132071535 1106 30-31 0.5461958918846221 1106 32-33 0.5305034970273752 1106 34-35 0.47731039998151203 1106 36-37 0.5716234882119693 1106 38-39 0.39872073277366127 1106 40-41 0.39711700322248333 1106 42-43 0.4899932948141128 1106 44-45 0.5153471633904516 1106 46-47 0.5125216296811317 1106 48-49 0.48816783981707346 1106 50-51 0.43117201656628623 1106 52-53 0.43907554444594155 1106 54-55 0.38568297338884605 1106 56-57 0.44392629910235826 1106 58-59 0.4078304249155096 1106 60-61 0.3677997983569483 1106 62-63 0.3663157737361331 1106 64-65 0.45715768602601514 1106 66-67 0.41302474642262155 1106 68-69 0.3998577553467939 1106 70-71 0.30886048820620005 1106 72-73 0.35877118022710874 1106 74-75 0.3331099065884473 1106 76-77 0.357306913187152 1106 78-79 0.36949600515205816 1106 80-81 0.34892897139673806 1106 82-83 0.3231154429084029 1106 84-85 0.3782763607034667 1106 86-87 0.33894594546328705 1106 88-89 0.36410043109719936 1106 90-91 0.42293856918206174 1106 92-93 0.32970966204432983 1106 94-95 0.39093882301103733 1106 96-97 0.3552673848924712 1106 98-99 0.3232076569170239 1106 100 0.30250538598940224 1107 1 0.03160656550761587 1107 2 0.12267240458333006 1107 3 0.12042314430425094 1107 4 -0.05669165445356583 1107 5 -0.030391769505797583 1107 6 0.08625111655083373 1107 7 0.06185557371820494 1107 8 0.08979142421821962 1107 9 0.09412934275593443 1107 10-11 0.11732371497600269 1107 12-13 0.05482532103792437 1107 14-15 0.17987205250618388 1107 16-17 0.14836826002279935 1107 18-19 0.18439100290312638 1107 20-21 0.1922235348145307 1107 22-23 0.16432888933097445 1107 24-25 0.2682606353585939 1107 26-27 0.2733744465000214 1107 28-29 0.2305499609284496 1107 30-31 0.21691857795072167 1107 32-33 0.3403386424742223 1107 34-35 0.2374846442696139 1107 36-37 0.21333941712987325 1107 38-39 0.2692912494573676 1107 40-41 0.2751077428973474 1107 42-43 0.16222962421022658 1107 44-45 0.1463341539427816 1107 46-47 0.23108926624421855 1107 48-49 0.24406320897735867 1107 50-51 0.2206599524873596 1107 52-53 0.23141057139252297 1107 54-55 0.23655581245195378 1107 56-57 0.2712590801868586 1107 58-59 0.20040474715045065 1107 60-61 0.28895224536852027 1107 62-63 0.186073867680129 1107 64-65 0.1667821643427274 1107 66-67 0.17853752150310953 1107 68-69 0.16480564156547928 1107 70-71 0.17163184439841928 1107 72-73 0.21345803482390124 1107 74-75 0.2983254153006527 1107 76-77 0.16657348309741593 1107 78-79 0.14570656995816478 1107 80-81 0.1341520265065128 1107 82-83 0.15186608901696985 1107 84-85 0.15644191647721328 1107 86-87 0.14512838620751722 1107 88-89 0.18926597408044188 1107 90-91 0.14461380924853628 1107 92-93 0.12528884993758993 1107 94-95 0.11742652795065212 1107 96-97 0.10931317188844147 1107 98-99 0.09528700168205972 1107 100 0.1556891306126893 1108 1 0.04731451325579883 1108 2 0.004583833997777731 1108 3 0.018681130946241353 1108 4 0.04676640729370973 1108 5 0.04622377192281135 1108 6 0.01069967390355231 1108 7 0.051044633382197446 1108 8 0.12881791671215836 1108 9 0.06927024562871509 1108 10-11 0.1225546547755414 1108 12-13 0.09013973499320116 1108 14-15 0.04630333564415423 1108 16-17 0.03257678447225487 1108 18-19 0.04633817885512315 1108 20-21 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2215 20-21 -0.8022498204554793 2215 22-23 -0.6994957179232344 2215 24-25 -0.6859424754139098 2215 26-27 -0.7079697371813012 2215 28-29 -0.7607740639675882 2215 30-31 -0.7176389601480295 2215 32-33 -0.6722242830184797 2215 34-35 -0.733620995806163 2215 36-37 -0.6703446645210889 2215 38-39 -0.8153548899170531 2215 40-41 -0.8214340092037347 2215 42-43 -0.837919425136775 2215 44-45 -0.7206785233835191 2215 46-47 -0.5886309775764715 2215 48-49 -0.6773032346254197 2215 50-51 -0.5962202219392694 2215 52-53 -0.5465585815041223 2215 54-55 -0.62652779074606 2215 56-57 -0.6212848259828689 2215 58-59 -0.582203421460882 2215 60-61 -0.5873974063176419 2215 62-63 -0.6039398462740166 2215 64-65 -0.617801274299957 2215 66-67 -0.5392687791981672 2215 68-69 -0.6179162712442405 2215 70-71 -0.4488260396402808 2215 72-73 -0.4164473352558815 2215 74-75 -0.4715518884486798 2215 76-77 -0.5089454318758655 2215 78-79 -0.4669682208323138 2215 80-81 -0.5037398328636442 2215 82-83 -0.48268440825528813 2215 84-85 -0.4981690773541345 2215 86-87 -0.48997335626066985 2215 88-89 -0.45662469855183474 2215 90-91 -0.48068118319848807 2215 92-93 -0.5396867556694502 2215 94-95 -0.5037483688057769 2215 96-97 -0.5028672458053087 2215 98-99 -0.48808916250395384 2215 100 -0.3360138059069371 2216 1 -0.2574681456986383 2216 2 -0.3173775783814996 2216 3 -0.3431183069610455 2216 4 -0.4667223177617714 2216 5 -0.45061038425425437 2216 6 -0.44114345193197835 2216 7 -0.5821837465642403 2216 8 -0.4993521935444889 2216 9 -0.6496016157629256 2216 10-11 -0.6416047607186286 2216 12-13 -0.6220781487321432 2216 14-15 -0.8486577017125043 2216 16-17 -0.8242111865671831 2216 18-19 -0.785448155320907 2216 20-21 -0.7866547675414921 2216 22-23 -0.8132729239902403 2216 24-25 -0.7794813893475521 2216 26-27 -0.9060631827818284 2216 28-29 -0.9114498434460714 2216 30-31 -0.8788801899589131 2216 32-33 -0.9062097933206914 2216 34-35 -0.902290959745919 2216 36-37 -0.8141930629350398 2216 38-39 -0.7427868182356079 2216 40-41 -0.831556171008188 2216 42-43 -0.8090574688630809 2216 44-45 -0.80445646618778 2216 46-47 -0.8072269039079245 2216 48-49 -0.6849172497009164 2216 50-51 -0.7852758254641614 2216 52-53 -0.7832215684722499 2216 54-55 -0.7274747024960035 2216 56-57 -0.6138191054503324 2216 58-59 -0.5815785406572971 2216 60-61 -0.6595505523602085 2216 62-63 -0.7109621985235606 2216 64-65 -0.8134140388162976 2216 66-67 -0.7333477544415032 2216 68-69 -0.6933260651442339 2216 70-71 -0.6741144613108006 2216 72-73 -0.6373601457410345 2216 74-75 -0.6961607472606062 2216 76-77 -0.7749434720949395 2216 78-79 -0.6183653348759535 2216 80-81 -0.6808513203521187 2216 82-83 -0.5967230114931681 2216 84-85 -0.59250145432474 2216 86-87 -0.6548015693697451 2216 88-89 -0.6597291039914097 2216 90-91 -0.723329587155412 2216 92-93 -0.6545570545205912 2216 94-95 -0.709453735911783 2216 96-97 -0.7017273368078101 2216 98-99 -0.6036090163767547 2216 100 -0.651733236818778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1037.0 1 270.0 2 171.0 3 178.0 4 159.0 5 184.0 6 291.0 7 640.0 8 1967.0 9 2713.0 10 3446.0 11 5094.0 12 7711.0 13 10172.0 14 12209.0 15 13196.0 16 13979.0 17 14973.0 18 16640.0 19 17995.0 20 19901.0 21 21222.0 22 23360.0 23 26200.0 24 31025.0 25 36722.0 26 39306.0 27 38213.0 28 41384.0 29 49358.0 30 56925.0 31 66962.0 32 83820.0 33 110483.0 34 159218.0 35 242573.0 36 443308.0 37 796576.0 38 781312.0 39 181376.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85477695663788 19.379515032796128 13.018965363725401 24.746742646840588 2 15.15293303631299 20.342461526410798 39.86978854509483 24.63481689218138 3 17.89703927757815 25.654866172070456 30.13905214032399 26.3090424100274 4 12.436446317558755 17.10774769577561 38.76681893139303 31.688987055272598 5 13.589344418455445 38.07793454927025 35.08137866853898 13.251342363735317 6 30.995247891942075 37.73043806529964 18.01889446711216 13.25541957564613 7 27.035179548087086 32.577948997831456 22.765431395296037 17.62144005878542 8 25.810128783692427 35.279205443139475 20.700537701742025 18.21012807142607 9 26.5051617670187 15.460375018175235 20.18682658896073 37.84763662584533 10-11 24.65086410022352 26.23878699534802 28.71186510089182 20.398483803536642 12-13 25.063692169646384 24.11998578090487 28.080620963788782 22.735701085659958 14-15 22.675690497231603 25.449638526472352 25.1739114694028 26.700759506893252 16-17 22.663426874997775 28.250450179893903 26.098251984411963 22.98787096069636 18-19 22.66984294910554 28.074637196779896 27.112627206476407 22.142892647638153 20-21 23.65473215561397 27.127594309214608 26.609945358288297 22.607728176883125 22-23 23.69938139930791 26.930117098367646 26.316071377436636 23.054430124887816 24-25 23.30332845130207 27.158125139750677 26.542621230886148 22.995925178061096 26-27 23.156307060769223 27.276203091568892 26.369387987340794 23.198101860321096 28-29 23.009096942703756 27.466463843032074 26.4317309385821 23.09270827568207 30-31 23.34009619155572 27.150721718597932 26.356198402169024 23.152983687677327 32-33 23.272976942556863 27.400364025842094 26.009714676271933 23.31694435532911 34-35 23.40715872647487 27.299567114470413 26.409519326709564 22.883754832345147 36-37 23.450376078981705 26.98704843551826 26.42515956293608 23.137415922563953 38-39 23.21602007480566 27.21836767552656 26.473560925951894 23.092051323715886 40-41 23.286247914944422 26.873500618560925 26.72721841677785 23.113033049716805 42-43 23.20262436920195 26.93887205903347 26.423727118313572 23.434776453451015 44-45 23.10635160159332 27.25516973658691 26.489345489452777 23.14913317236699 46-47 23.236980067041692 27.08920640620618 26.372284685321013 23.301528841431114 48-49 23.188947357720473 26.94635837982157 26.498391393976323 23.366302868481636 50-51 23.10521794347266 27.066055208972834 26.37898891039171 23.449737937162794 52-53 23.04215732076594 27.162761320241174 26.346796899626078 23.448284459366807 54-55 23.024041469353254 27.045525234550194 26.375099868583035 23.555333427513514 56-57 23.186044244158946 26.964192140515653 26.382509389124774 23.467254226200627 58-59 23.004452153853165 26.81888791284529 26.588659362813218 23.58800057048833 60-61 22.990579363644756 27.059092758792946 26.47935215798436 23.47097571957794 62-63 23.019115292367463 26.932317697848045 26.470170506193647 23.57839650359084 64-65 22.78589616469108 26.89802325527898 26.442368555521767 23.873712024508173 66-67 22.966772620393428 26.739966045533535 26.40396850804122 23.889292826031816 68-69 22.767264627299006 26.733475124698185 26.59186185240905 23.907398395593752 70-71 22.77324750619037 26.550857806397378 26.636314902423376 24.039579784988877 72-73 22.882960404140483 26.589594946789525 26.638065922010405 23.889378727059583 74-75 22.77521159219649 26.90641500551438 26.601718086930788 23.71665531535834 76-77 22.847990539665314 26.57151320831555 26.65453453146826 23.925961720550877 78-79 22.70669002822236 26.618667129741976 26.686184103604194 23.988458738431465 80-81 22.667271210740502 26.701252326406305 26.78555208923902 23.845924373614174 82-83 22.810031799274963 26.377665554743363 27.108150929674252 23.704151716307425 84-85 22.81781389497106 26.55920261437715 26.720842456587214 23.902141034064584 86-87 22.52883505017092 26.596678246149825 26.980542045663835 23.893944658015425 88-89 22.821538521815633 26.18505146254792 26.831702211779458 24.16170780385699 90-91 22.6517629068001 26.268848852402378 27.009342132680008 24.07004610811751 92-93 22.8478796597578 26.255436303720085 26.951259435713677 23.94542460080843 94-95 22.410619361862402 26.06482407615719 27.14840608065553 24.376150481324878 96-97 22.601096366911918 25.915588058089046 27.145572832419695 24.33774274257934 98-99 22.408173684866693 26.2300006530399 26.943788106362387 24.41803755573102 100 22.656763454543345 25.9442398862599 26.814233920433654 24.584762738763096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1462.0 1 1033.0 2 590.5 3 561.5 4 546.5 5 533.0 6 479.5 7 441.5 8 426.0 9 425.0 10 399.5 11 357.5 12 394.0 13 438.0 14 442.5 15 513.5 16 592.0 17 676.5 18 919.5 19 1185.5 20 1502.5 21 2039.0 22 3036.5 23 4523.0 24 6057.5 25 8356.0 26 11745.5 27 15835.5 28 20329.0 29 25671.0 30 32355.0 31 39612.0 32 48983.5 33 56696.0 34 62495.5 35 69603.5 36 76825.0 37 85999.0 38 92138.5 39 94725.5 40 96278.0 41 97974.0 42 101463.5 43 106419.5 44 112185.0 45 120329.5 46 128875.5 47 133202.5 48 135612.0 49 134817.0 50 132489.5 51 133147.0 52 132497.5 53 130633.5 54 127531.0 55 118104.5 56 108133.0 57 97437.5 58 85740.0 59 76343.0 60 64936.5 61 53206.5 62 43299.0 63 33935.5 64 25094.0 65 17702.0 66 13191.0 67 10317.5 68 7766.0 69 5820.5 70 4442.0 71 3304.5 72 2534.5 73 2019.0 74 1587.0 75 1200.0 76 834.0 77 569.5 78 359.0 79 223.0 80 166.0 81 114.0 82 77.5 83 61.0 84 42.5 85 29.5 86 25.0 87 26.0 88 22.5 89 19.5 90 23.0 91 16.0 92 8.5 93 9.0 94 6.5 95 6.0 96 7.5 97 5.5 98 5.0 99 7.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09390751636709863 2 0.06235791187875227 3 0.07650186050745642 4 0.07940774513138305 5 0.079022270640454 6 0.08940043001162058 7 0.08670210857511727 8 0.08720619060171678 9 0.07404075414229405 10-11 0.08062347237200543 12-13 0.06971157909032172 14-15 0.07854784049777211 16-17 0.08014904222932352 18-19 0.08004526063561185 20-21 0.07183168879043145 22-23 0.07479687718219333 24-25 0.07788067310962568 26-27 0.07206890386177241 28-29 0.08031212759087042 30-31 0.08410756873232562 32-33 0.0867614123429525 34-35 0.08851087349409201 36-37 0.08980073044450843 38-39 0.0910164576851308 40-41 0.0875471872667694 42-43 0.09263248535864102 44-45 0.08971177479275558 46-47 0.08664280480728202 48-49 0.0962351892546317 50-51 0.09315139332719935 52-53 0.09859251402608239 54-55 0.09011207522564343 56-57 0.09715439765607788 58-59 0.10151322459196785 60-61 0.10004545633804571 62-63 0.1011277501010388 64-65 0.10284755936826068 66-67 0.1050714506620821 68-69 0.1017800915472264 70-71 0.10452289080960615 72-73 0.10201730661856735 74-75 0.10513075442991733 76-77 0.10238795516753758 78-79 0.10925236629446633 80-81 0.10487871341661756 82-83 0.10910410687487823 84-85 0.10548657703692875 86-87 0.10180974343114403 88-89 0.10126118357866808 90-91 0.10167630995351475 92-93 0.09547906621473243 94-95 0.09562732563432051 96-97 0.09361099752792244 98-99 0.10695434529085088 100 0.24628854781974147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3372467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.86115746358994 #Duplication Level Percentage of deduplicated Percentage of total 1 71.23611076618582 41.218038221360125 2 14.955199690071893 17.306503283333626 3 6.017327111351705 10.445085344995492 4 2.916638195617578 6.750402476037981 5 1.6002277305780275 4.629551434828922 6 0.931185600054225 3.2327685979539007 7 0.6180815236965295 2.503403865755834 8 0.4111341054427714 1.9030956170941107 9 0.28104645122088645 1.4635505671807372 >10 1.003565686148041 8.96580094957494 >50 0.021925521431281018 0.8470964819688854 >100 0.007395341754657432 0.6591176605101644 >500 8.11382233204154E-5 0.026478398549441833 >1k 8.113822332040074E-5 0.04910710085587083 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7791130350571258E-4 2 0.0 0.0 0.0 0.0 1.7791130350571258E-4 3 0.0 0.0 0.0 0.0 1.7791130350571258E-4 4 0.0 0.0 0.0 2.9651883917618764E-5 1.7791130350571258E-4 5 0.0 0.0 0.0 2.9651883917618764E-5 1.7791130350571258E-4 6 0.0 0.0 0.0 2.9651883917618764E-5 3.261707230938064E-4 7 0.0 0.0 0.0 2.9651883917618764E-5 3.261707230938064E-4 8 0.0 0.0 0.0 2.9651883917618764E-5 3.261707230938064E-4 9 0.0 0.0 0.0 2.9651883917618764E-5 3.261707230938064E-4 10-11 0.0 0.0 0.0 2.9651883917618764E-5 3.706485489702345E-4 12-13 0.0 0.0 0.0 2.9651883917618764E-5 7.41297097940469E-4 14-15 0.0 0.0 0.0 2.9651883917618764E-5 8.450786916521347E-4 16-17 0.0 0.0 0.0 2.9651883917618764E-5 9.785121692814191E-4 18-19 1.4825941958809382E-5 0.0 0.0 2.9651883917618764E-5 0.001126771588869513 20-21 2.9651883917618764E-5 0.0 0.0 4.4477825876428146E-5 0.001393638544128082 22-23 2.9651883917618764E-5 0.0 0.0 1.3343347762928445E-4 0.001512246079798557 24-25 2.9651883917618764E-5 0.0 0.0 3.8547449092904396E-4 0.001512246079798557 26-27 2.9651883917618764E-5 0.0 0.0 9.192084014461817E-4 0.0017791130350571257 28-29 2.9651883917618764E-5 0.0 0.0 0.004596042007230908 0.0018532427448511727 30-31 2.9651883917618764E-5 0.0 0.0 0.015685846592420324 0.0018680686868099822 32-33 2.9651883917618764E-5 0.0 0.0 0.04017830270837343 0.001897720570727601 34-35 2.9651883917618764E-5 0.0 0.0 0.0791705300600421 0.0020756318742333134 36-37 2.9651883917618764E-5 0.0 0.0 0.1420770017912703 0.0020756318742333134 38-39 2.9651883917618764E-5 0.0 0.0 0.23488739845341702 0.002253543177739026 40-41 2.9651883917618764E-5 0.0 0.0 0.3362523636257968 0.002253543177739026 42-43 2.9651883917618764E-5 0.0 0.0 0.441472073707467 0.002268369119697835 44-45 2.9651883917618764E-5 0.0 0.0 0.5773073539340785 0.002372150713409501 46-47 2.9651883917618764E-5 0.0 0.0 0.7421273506901624 0.002520410132997595 48-49 2.9651883917618764E-5 0.0 0.0 0.9158280866795732 0.0026983214365033075 50-51 2.9651883917618764E-5 0.0 0.0 1.1003517602989148 0.0029058846239266387 52-53 2.9651883917618764E-5 0.0 0.0 1.28545364565465 0.0031134478113499704 54-55 2.9651883917618764E-5 0.0 0.0 1.4939063895955096 0.0031727515791852077 56-57 2.9651883917618764E-5 0.0 0.0 1.744094160150418 0.003291359114855683 58-59 2.9651883917618764E-5 0.0 0.0 2.023726251435522 0.0034396185344437767 60-61 2.9651883917618764E-5 0.0 0.0 2.318258414389229 0.003454444476402586 62-63 2.9651883917618764E-5 0.0 0.0 2.611886194883449 0.0036175298379494893 64-65 2.9651883917618764E-5 0.0 0.0 2.9257662120934023 0.0036175298379494893 66-67 2.9651883917618764E-5 0.0 0.0 3.2786532825969834 0.003647181721867108 68-69 2.9651883917618764E-5 0.0 0.0 3.67044362480048 0.003721311431661155 70-71 2.9651883917618764E-5 0.0 0.0 4.09966947044997 0.0037509633155787736 72-73 2.9651883917618764E-5 0.0 0.0 4.535833856936184 0.0038843967932080583 74-75 2.9651883917618764E-5 0.0 0.0 4.972991581533638 0.004032656212796151 76-77 2.9651883917618764E-5 0.0 0.0 5.458363269381138 0.0040919599806313895 78-79 2.9651883917618764E-5 0.0 0.0 5.990836974831778 0.004166089690425436 80-81 2.9651883917618764E-5 0.0 0.0 6.555853622882003 0.004225393458260673 82-83 2.9651883917618764E-5 0.0 0.0 7.150996584992529 0.004344000993931149 84-85 2.9651883917618764E-5 0.0 0.0 7.741217334372731 0.004373652877848768 86-87 2.9651883917618764E-5 1.4825941958809382E-5 0.0 8.356390144069607 0.004581216065272099 88 2.9651883917618764E-5 2.9651883917618764E-5 0.0 8.831398498487902 0.004773953310736621 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2485 0.0 43.522186 1 GTATCAA 4490 0.0 36.445354 1 ATCAACG 4870 0.0 31.758986 3 CAACGCA 4945 0.0 31.751226 5 TATCAAC 5745 0.0 28.394821 2 AACGCAG 5675 0.0 28.32162 6 GTACATG 10885 0.0 27.647816 1 TCAACGC 5710 0.0 27.333094 4 TACATGG 11060 0.0 26.48089 2 ACATGGG 10755 0.0 25.876825 3 ACGCAGA 6260 0.0 24.848783 7 CGCAGAG 6590 0.0 23.532097 8 TGATCGC 770 0.0 23.190607 9 CATGGGG 8040 0.0 23.095524 4 GTGATCG 815 0.0 22.487392 8 GTATAAG 1535 0.0 20.524647 1 GTACTAG 1245 0.0 20.39547 1 ATGGGGG 5235 0.0 20.294207 5 ACGGGCG 690 0.0 19.757376 5 GTATAAT 1205 0.0 19.51157 1 >>END_MODULE