FastQCFastQC Report
Mon 16 Jan 2017
ERR1146602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372467
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83230.24679262984634098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74750.22164783228420026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.12851126489895973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50100.060.2662051
GTATCAA94700.043.2061771
ATCAACG117800.034.212113
CAACGCA118300.034.0095985
TATCAAC123450.032.8387262
TCAACGC126500.032.0078854
AACGCAG129300.031.2602926
ACGCAGA144300.027.8788477
CGCAGAG147900.027.1049258
GTACATG103400.025.8347551
ACATGGG101150.024.6872653
TACATGG107100.024.4149282
GCAGAGT163550.024.3388619
AGAGTAC152800.021.71444510-11
GAGTACT110750.021.6633612-13
CATGGGG80250.021.5062834
CAGAGTA160450.021.32352610-11
GTGGTAT16950.021.0872751
GTACTTT118600.020.09079414-15
TAGTACT17500.019.8855694
Produced by FastQC (version 0.11.5)