##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3372467
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28669101877053	33.0	31.0	34.0	30.0	34.0
2	32.34895463765842	34.0	31.0	34.0	30.0	34.0
3	32.47239750603935	34.0	31.0	34.0	30.0	34.0
4	36.013158023488444	37.0	35.0	37.0	35.0	37.0
5	36.063531829963054	37.0	35.0	37.0	35.0	37.0
6	36.137698604612	37.0	36.0	37.0	35.0	37.0
7	36.10027436888189	37.0	36.0	37.0	35.0	37.0
8	36.10159654638578	37.0	36.0	37.0	35.0	37.0
9	37.88813352361936	39.0	38.0	39.0	35.0	39.0
10-11	37.83744970669839	39.0	38.0	39.0	35.0	39.0
12-13	37.82189951747489	39.0	38.0	39.0	35.0	39.0
14-15	39.187839940316685	40.0	39.0	41.0	36.0	41.0
16-17	39.129043516215276	40.0	39.0	41.0	36.0	41.0
18-19	39.22442398991599	40.0	39.0	41.0	36.0	41.0
20-21	39.278996354893906	41.0	39.0	41.0	36.0	41.0
22-23	39.252196537430905	40.5	39.0	41.0	36.0	41.0
24-25	39.24786528674706	41.0	39.0	41.0	36.0	41.0
26-27	39.176765702970556	41.0	39.0	41.0	36.0	41.0
28-29	39.11698809803032	40.0	39.0	41.0	35.0	41.0
30-31	38.98369843203803	40.0	38.5	41.0	35.0	41.0
32-33	38.89141376327774	40.0	38.0	41.0	35.0	41.0
34-35	38.830593005061274	40.0	38.0	41.0	35.0	41.0
36-37	38.76689764495842	40.0	38.0	41.0	35.0	41.0
38-39	38.68337347704218	40.0	38.0	41.0	35.0	41.0
40-41	38.58711085979492	40.0	38.0	41.0	35.0	41.0
42-43	38.483908070857325	40.0	38.0	41.0	34.5	41.0
44-45	38.35012114277175	40.0	38.0	41.0	34.0	41.0
46-47	38.12382330205158	40.0	38.0	41.0	33.5	41.0
48-49	37.85825480278977	40.0	37.5	41.0	33.0	41.0
50-51	37.75974694489227	40.0	37.0	41.0	33.0	41.0
52-53	37.65062445384936	40.0	37.0	41.0	33.0	41.0
54-55	37.482930596503984	40.0	36.5	41.0	33.0	41.0
56-57	37.32303785329849	39.5	36.0	41.0	32.5	41.0
58-59	37.14027980110703	39.0	35.5	41.0	32.0	41.0
60-61	36.92384995909522	39.0	35.0	41.0	32.0	41.0
62-63	36.66829608710775	39.0	35.0	41.0	32.0	41.0
64-65	36.38249388355764	38.0	35.0	40.0	31.0	41.0
66-67	36.09884040377564	37.0	35.0	40.0	31.0	41.0
68-69	35.79199396169036	37.0	35.0	39.5	31.0	41.0
70-71	35.47079882471793	36.0	35.0	39.0	31.0	41.0
72-73	35.141718955292966	36.0	35.0	39.0	31.0	41.0
74-75	34.8296788374801	35.0	35.0	38.0	30.0	40.0
76-77	34.542036734532914	35.0	34.0	37.0	30.0	39.0
78-79	34.26474743859613	35.0	34.0	37.0	30.0	39.0
80-81	34.015965167338926	35.0	34.0	36.0	30.0	39.0
82-83	33.778652837818726	35.0	34.0	36.0	30.0	37.0
84-85	33.581435340953675	35.0	34.0	35.5	30.0	37.0
86-87	33.40931341952346	35.0	34.0	35.0	29.0	37.0
88-89	33.22221729671484	35.0	34.0	35.0	29.0	36.0
90-91	33.15147071861637	35.0	34.0	35.0	29.0	36.0
92-93	33.06735380954061	35.0	34.0	35.0	29.0	36.0
94-95	32.99914113318232	35.0	34.0	35.0	29.0	36.0
96-97	32.94668383708425	35.0	34.0	35.0	29.0	35.0
98-99	32.8829355483686	35.0	34.0	35.0	29.0	35.0
100	32.156508573693976	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36866644215368893
1101	2	0.32625805439897704
1101	3	0.3598536151410414
1101	4	0.27952333171405996
1101	5	0.255149222235282
1101	6	0.26725198691767105
1101	7	0.2881140187315978
1101	8	0.28605885366251727
1101	9	0.2940315683047672
1101	10-11	0.3077927142681176
1101	12-13	0.3756545636369495
1101	14-15	0.5138388279687547
1101	16-17	0.5558138459363846
1101	18-19	0.4822941242201466
1101	20-21	0.46587218546896025
1101	22-23	0.4354047537081769
1101	24-25	0.4214919961600643
1101	26-27	0.4181109800225329
1101	28-29	0.45466184439369783
1101	30-31	0.5086274721930621
1101	32-33	0.48355728985428215
1101	34-35	0.45769967963219926
1101	36-37	0.44723509009967444
1101	38-39	0.4375941705613684
1101	40-41	0.47168486199011994
1101	42-43	0.505425199726055
1101	44-45	0.48478160376001256
1101	46-47	0.5262031592781611
1101	48-49	0.6080759052448172
1101	50-51	0.5510481209194893
1101	52-53	0.5535685570287328
1101	54-55	0.5956767102939793
1101	56-57	0.5055342435576691
1101	58-59	0.5325856512060909
1101	60-61	0.48528789733091315
1101	62-63	0.44171959421228735
1101	64-65	0.4329304734816475
1101	66-67	0.4019039956302919
1101	68-69	0.39520126776741904
1101	70-71	0.43725242414264187
1101	72-73	0.4309773428228709
1101	74-75	0.4982857991871157
1101	76-77	0.44301805173897435
1101	78-79	0.4051451667081025
1101	80-81	0.3630668004892499
1101	82-83	0.39189949793344425
1101	84-85	0.3220806475664517
1101	86-87	0.29135164341283115
1101	88-89	0.4211605326027268
1101	90-91	0.38035680029221197
1101	92-93	0.4144050286774501
1101	94-95	0.46328993537118635
1101	96-97	0.3682000936126215
1101	98-99	0.3466212718880186
1101	100	0.4116992338458232
1102	1	0.28303245218704376
1102	2	0.23262657127413888
1102	3	0.27427304967184796
1102	4	0.24294775638401944
1102	5	0.1991187822580045
1102	6	0.25408867019815773
1102	7	0.2512625024476165
1102	8	0.2135572301656694
1102	9	0.24889484568364395
1102	10-11	0.30165294242668494
1102	12-13	0.3415638909634495
1102	14-15	0.4890567104494181
1102	16-17	0.5669454478536693
1102	18-19	0.48106289931371293
1102	20-21	0.41680627941645554
1102	22-23	0.4395627249813785
1102	24-25	0.44890925133096715
1102	26-27	0.4372671940236046
1102	28-29	0.4250688465987409
1102	30-31	0.4668225674988591
1102	32-33	0.48720715049254437
1102	34-35	0.5089965507460263
1102	36-37	0.5201762884779271
1102	38-39	0.4537364381145039
1102	40-41	0.42156624077023963
1102	42-43	0.5206473073652873
1102	44-45	0.5342653595877991
1102	46-47	0.5060389645647021
1102	48-49	0.5251419713995844
1102	50-51	0.5407454120663076
1102	52-53	0.46414647909831075
1102	54-55	0.4704997309441694
1102	56-57	0.40636576931935764
1102	58-59	0.46438176739923875
1102	60-61	0.4115192613636367
1102	62-63	0.4214108793839486
1102	64-65	0.43520221324955344
1102	66-67	0.4618819409018613
1102	68-69	0.48978865556205875
1102	70-71	0.3887885710805534
1102	72-73	0.40865589858062634
1102	74-75	0.4188949840054832
1102	76-77	0.4087139807323368
1102	78-79	0.40068933673841656
1102	80-81	0.3735422613932613
1102	82-83	0.3540629888818856
1102	84-85	0.3572939838278586
1102	86-87	0.3662511193133824
1102	88-89	0.4225518169610325
1102	90-91	0.3777809090384636
1102	92-93	0.4154320375862426
1102	94-95	0.40808674034651204
1102	96-97	0.42074000466596573
1102	98-99	0.3954109801380312
1102	100	0.5112652026121793
1103	1	0.23349903629561197
1103	2	0.22974392324536552
1103	3	0.2128851439863908
1103	4	0.19849073428027708
1103	5	0.1633999741932044
1103	6	0.2071001646409769
1103	7	0.18292381041172234
1103	8	0.19333015062586156
1103	9	0.2406026106553938
1103	10-11	0.26432920896886003
1103	12-13	0.2248317252179035
1103	14-15	0.38372510264240844
1103	16-17	0.4202018279459452
1103	18-19	0.3610720295926697
1103	20-21	0.2924466125270442
1103	22-23	0.34816997529046034
1103	24-25	0.3434526702486096
1103	26-27	0.3298114536705583
1103	28-29	0.35710775976503584
1103	30-31	0.3238557225989567
1103	32-33	0.3153492729932097
1103	34-35	0.33393285052490285
1103	36-37	0.35878363174233385
1103	38-39	0.3585995510645219
1103	40-41	0.3654990981034345
1103	42-43	0.42444938687927447
1103	44-45	0.492062607490368
1103	46-47	0.4684682797834512
1103	48-49	0.481193414944201
1103	50-51	0.513207809053732
1103	52-53	0.45696309785855505
1103	54-55	0.488387434709999
1103	56-57	0.4523835113931085
1103	58-59	0.4400431703843424
1103	60-61	0.4528949176024355
1103	62-63	0.4034058453364793
1103	64-65	0.38914780546279104
1103	66-67	0.3560851433162142
1103	68-69	0.36657719355032015
1103	70-71	0.35925521715933684
1103	72-73	0.32243156845287757
1103	74-75	0.33535324138226486
1103	76-77	0.25197332506675707
1103	78-79	0.2666263729300553
1103	80-81	0.28737070923551045
1103	82-83	0.2775767622795158
1103	84-85	0.24390221946342194
1103	86-87	0.28832718362607324
1103	88-89	0.3131775819059257
1103	90-91	0.2840827639104049
1103	92-93	0.29762768376521365
1103	94-95	0.2882661854445132
1103	96-97	0.2851521603310303
1103	98-99	0.23846324065670643
1103	100	0.29885587278911885
1104	1	0.20191685393552916
1104	2	0.19590756870476156
1104	3	0.1491306353774462
1104	4	0.14511230502040462
1104	5	0.07794080536881864
1104	6	0.12217647263111786
1104	7	0.10973904831932657
1104	8	0.13586911261901236
1104	9	0.17174269391379227
1104	10-11	0.17753303737367787
1104	12-13	0.1937385139892953
1104	14-15	0.3315392134232198
1104	16-17	0.38547720468268665
1104	18-19	0.331058584667403
1104	20-21	0.2743431407404202
1104	22-23	0.256733737329526
1104	24-25	0.23518186348389492
1104	26-27	0.25597475720633156
1104	28-29	0.256874457951092
1104	30-31	0.3114553332944894
1104	32-33	0.3019032486897615
1104	34-35	0.2721047558820686
1104	36-37	0.33177184881009936
1104	38-39	0.3153037777696568
1104	40-41	0.3131777012965884
1104	42-43	0.28553023594075455
1104	44-45	0.3644752124232653
1104	46-47	0.3166196127360621
1104	48-49	0.45374696160801875
1104	50-51	0.41913743628777667
1104	52-53	0.35704587186861403
1104	54-55	0.3859300622057873
1104	56-57	0.30254743120550387
1104	58-59	0.26128248883287597
1104	60-61	0.26709522948562636
1104	62-63	0.26408450673806527
1104	64-65	0.29246444423961293
1104	66-67	0.28994830222644197
1104	68-69	0.2226664461989074
1104	70-71	0.2781660357448459
1104	72-73	0.2855370327586897
1104	74-75	0.2804843304212028
1104	76-77	0.23484362326079378
1104	78-79	0.27658086859475617
1104	80-81	0.2321141423684807
1104	82-83	0.22216730670739793
1104	84-85	0.20778956946987392
1104	86-87	0.251790344815781
1104	88-89	0.27028476966961534
1104	90-91	0.24387315658933062
1104	92-93	0.21319149749987787
1104	94-95	0.2310067195536334
1104	96-97	0.2263135161233194
1104	98-99	0.20661696300413723
1104	100	0.3092637021887654
1105	1	0.13338777755666342
1105	2	0.14910223541841816
1105	3	0.1214648575079238
1105	4	0.1365761709167117
1105	5	0.1262032300166709
1105	6	0.11746560650385618
1105	7	0.10745197418206232
1105	8	0.12499382887833832
1105	9	0.13949159749640216
1105	10-11	0.1699761507900206
1105	12-13	0.20967283026472927
1105	14-15	0.293689253478874
1105	16-17	0.3617976411225712
1105	18-19	0.24734658955589595
1105	20-21	0.2262763532040566
1105	22-23	0.25687576499529996
1105	24-25	0.2386000851260306
1105	26-27	0.26872558490785536
1105	28-29	0.2261593134518094
1105	30-31	0.27109080322316714
1105	32-33	0.2134829046956881
1105	34-35	0.209254032897924
1105	36-37	0.21828541140988733
1105	38-39	0.23940059037148842
1105	40-41	0.23586174964278683
1105	42-43	0.2521158578166194
1105	44-45	0.2990668508286163
1105	46-47	0.33838087850737963
1105	48-49	0.3846153984431169
1105	50-51	0.328934725718689
1105	52-53	0.32075652497405116
1105	54-55	0.3810244636018467
1105	56-57	0.39121603845806874
1105	58-59	0.3686628772311451
1105	60-61	0.22904483393208608
1105	62-63	0.3362809726523679
1105	64-65	0.3172155280707827
1105	66-67	0.25076030872848776
1105	68-69	0.24033298081511134
1105	70-71	0.20822506041321276
1105	72-73	0.20629157371080709
1105	74-75	0.23070357456813895
1105	76-77	0.17736804368092862
1105	78-79	0.216821990744684
1105	80-81	0.22828749777385582
1105	82-83	0.2823171441248604
1105	84-85	0.2535845043330198
1105	86-87	0.25567059885612053
1105	88-89	0.3075468349807906
1105	90-91	0.28761582904733984
1105	92-93	0.343955830533865
1105	94-95	0.3770018383015028
1105	96-97	0.29019599477864944
1105	98-99	0.31213490498038965
1105	100	0.2562238617569079
1106	1	0.08499340538256206
1106	2	0.09980914823504605
1106	3	0.10422876297608497
1106	4	0.10678890114803608
1106	5	0.07328147126765572
1106	6	0.11368802682924439
1106	7	0.10740144769916782
1106	8	0.0915536928384526
1106	9	0.12082717577276014
1106	10-11	0.1481198215466364
1106	12-13	0.1752673483703049
1106	14-15	0.264199074850211
1106	16-17	0.32969134317202275
1106	18-19	0.2371299362713728
1106	20-21	0.20618848902412168
1106	22-23	0.23679786109636325
1106	24-25	0.23555754365920478
1106	26-27	0.2621491662738151
1106	28-29	0.2658624818133788
1106	30-31	0.2933876745500825
1106	32-33	0.25421846953902616
1106	34-35	0.35749629135538896
1106	36-37	0.30253542133200284
1106	38-39	0.3555096085091165
1106	40-41	0.32433991792803596
1106	42-43	0.31737317652424224
1106	44-45	0.31667857388853093
1106	46-47	0.3867864703083512
1106	48-49	0.4080476273173943
1106	50-51	0.3981301334567249
1106	52-53	0.3538066072513075
1106	54-55	0.33219264046591235
1106	56-57	0.37315785820522507
1106	58-59	0.34879749748875355
1106	60-61	0.3915966579228538
1106	62-63	0.40425023545419236
1106	64-65	0.39487538104024367
1106	66-67	0.4310035380271344
1106	68-69	0.3558485417506603
1106	70-71	0.37740753123455306
1106	72-73	0.3234047102910651
1106	74-75	0.32901212041291217
1106	76-77	0.3371002304243689
1106	78-79	0.29548680172067776
1106	80-81	0.30464930860636485
1106	82-83	0.29531241051086
1106	84-85	0.3042603151227894
1106	86-87	0.28919507290749635
1106	88-89	0.3431386206874407
1106	90-91	0.3021400939709693
1106	92-93	0.24290721520372927
1106	94-95	0.2893211794117505
1106	96-97	0.30065054994716434
1106	98-99	0.28536174599140907
1106	100	0.3046818881405855
1107	1	0.0803937143108584
1107	2	0.05347328915329541
1107	3	0.12417382399807053
1107	4	0.06920633656163488
1107	5	0.046296290947879015
1107	6	0.13528411624540126
1107	7	0.07719764982223865
1107	8	0.05676209153502754
1107	9	0.15476098566523433
1107	10-11	0.1377209534310282
1107	12-13	0.14252012108830314
1107	14-15	0.22165943745348216
1107	16-17	0.3024473432352295
1107	18-19	0.19964405285004716
1107	20-21	0.16679095187335946
1107	22-23	0.12699258523905144
1107	24-25	0.21345491265246608
1107	26-27	0.18241056656317767
1107	28-29	0.1770931841399701
1107	30-31	0.2001956461942811
1107	32-33	0.2209079710178301
1107	34-35	0.16711930252601093
1107	36-37	0.14897127206230465
1107	38-39	0.14075835748494114
1107	40-41	0.14345995380163856
1107	42-43	0.14199365592739355
1107	44-45	0.11417325266254608
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2216	58-59	-0.7611751364373873
2216	60-61	-0.7625970044790691
2216	62-63	-0.6965525546898661
2216	64-65	-0.7145661743299243
2216	66-67	-0.7000041988011603
2216	68-69	-0.667887953884545
2216	70-71	-0.5938966843882838
2216	72-73	-0.5795605974278359
2216	74-75	-0.6076151474237648
2216	76-77	-0.6394820250286983
2216	78-79	-0.6371561424842369
2216	80-81	-0.6323479080066292
2216	82-83	-0.6285476612698631
2216	84-85	-0.6532203947838298
2216	86-87	-0.6154727528540391
2216	88-89	-0.520843156054255
2216	90-91	-0.5867524766467511
2216	92-93	-0.5692879701344964
2216	94-95	-0.5226810400210198
2216	96-97	-0.5754151806074645
2216	98-99	-0.548196779203316
2216	100	-0.7040380916922011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	26.0
8	123.0
9	179.0
10	217.0
11	148.0
12	169.0
13	233.0
14	352.0
15	686.0
16	1315.0
17	2208.0
18	3816.0
19	5891.0
20	8548.0
21	12566.0
22	18107.0
23	21483.0
24	20266.0
25	18524.0
26	18421.0
27	21303.0
28	26624.0
29	33542.0
30	41275.0
31	53014.0
32	67761.0
33	89622.0
34	127402.0
35	198314.0
36	361836.0
37	818860.0
38	1066219.0
39	332723.0
40	690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58333820131996	19.50140153535804	13.277594145131633	24.637666118190367
2	15.5283750288004	20.607742555708203	39.3311012590927	24.532781156398702
3	17.808381248448832	25.92999703183762	30.670487422078324	25.591134297635225
4	12.602032915164271	17.554467494539754	38.0838430652625	31.75965652503348
5	13.105625995026093	38.04158404743922	35.10506912141772	13.747720836116962
6	30.561829671611925	38.15333712300816	17.690279187538792	13.594554017841126
7	26.538918405700755	32.936165998411845	22.811556884487494	17.713358711399906
8	25.967009245476596	35.076502019297486	20.41557201567995	18.54091671954597
9	26.565041723178233	14.7721281697132	20.37369687344513	38.289133233663435
10-11	24.73184294925016	25.92344944230687	28.668578819521866	20.676128788921098
12-13	25.665143647069044	23.755903319439447	28.401182279915567	22.177770753575942
14-15	22.90643151130827	24.856088223882498	26.14223926086123	26.095241003948
16-17	22.21746143764492	27.80342835793456	26.930875384733994	23.04823481968652
18-19	22.13443112644972	26.886670068730183	29.10868110339535	21.870217701424746
20-21	23.034094256728658	25.949029187748586	28.949732383739523	22.067144171783234
22-23	23.03663757124977	25.840697625803305	28.649235114828404	22.47342968811852
24-25	22.705692894845228	26.05220451378768	28.84653875041624	22.395563840950853
26-27	22.615957285773185	26.18630409053096	28.675718715080194	22.522019908615658
28-29	22.470438179296963	26.31625844170728	28.771494097865762	22.44180928113
30-31	22.79339117439495	26.01191711688204	28.655981093919575	22.538710614803435
32-33	22.721086256865163	26.321111994738562	28.272712931976695	22.685088816419576
34-35	22.914357522783057	26.20598838474681	28.579417647997218	22.300236444472908
36-37	22.94059049677454	25.962444982758164	28.523405206072532	22.573559314394764
38-39	22.799485654990022	26.06428363758099	28.602620443420196	22.533610264008793
40-41	22.800160120980294	25.853059348544825	28.74177526723895	22.605005263235928
42-43	22.897688843211807	25.90341136028907	28.306681725870114	22.89221807062901
44-45	22.806880988503465	26.28489987179949	28.19570570547864	22.7125134342184
46-47	23.102108930939874	26.150144686367575	27.80015341884739	22.94759296384516
48-49	23.16686647118145	26.242022521818626	27.61609245231156	22.97501855468837
50-51	23.043931934693504	26.411614998753137	27.33365515511345	23.210797911439904
52-53	22.970711805746028	26.55011147678647	27.307972747417235	23.171203970050268
54-55	22.925964731611572	26.472877980378378	27.278226371394	23.32293091661605
56-57	23.11206860509284	26.33827344482264	27.24148626181296	23.30817168827156
58-59	22.778701773290543	26.30488214584672	27.5717872359731	23.344628844889638
60-61	22.858719077321965	26.529958688713585	27.36807490354176	23.243247330422683
62-63	22.83024048624461	26.399179352513997	27.385166135451904	23.38541402578949
64-65	22.58790224980122	26.343639726982147	27.400763272199306	23.667694751017322
66-67	22.757941958898662	26.278692114462	27.317041276436317	23.64632465020302
68-69	22.596994814627454	26.252494165084446	27.440693802668804	23.709817217619296
70-71	22.66200244874406	26.1354056425492	27.37458934312989	23.82800256557685
72-73	22.794602341215374	26.1797630199229	27.29019013738487	23.735444501476863
74-75	22.691131389653435	26.51352196054639	27.098244971038138	23.697101678762035
76-77	22.752271025248046	26.29283011504986	27.068586749094575	23.88631211060752
78-79	22.540284528828355	26.441803246484163	27.054390507699594	23.963521716987888
80-81	22.59479932327658	26.507634784627626	26.984887587616807	23.912678304478984
82-83	22.737066680899673	26.213226763688336	27.278256041429916	23.771450513982074
84-85	22.76325274214348	26.4392268772908	26.798268204182534	23.999252176383184
86-87	22.39772842847684	26.540734127272408	27.039864289257686	24.021673154993064
88-89	22.64486205498823	26.24500106302004	26.81253812120326	24.297598760788468
90-91	22.413243009714552	26.257685029293103	26.984571527185153	24.34450043380719
92-93	22.595243474945008	26.275907630686422	26.871540731963773	24.257308162404794
94-95	22.178622408646966	26.156112470815124	27.015246410193612	24.650018710344302
96-97	22.335303311502564	26.02961283123847	26.993227786151163	24.6418560711078
98-99	22.157292756065818	26.37051911318227	26.744444638829247	24.72774349192267
100	22.335938969824984	26.041092337577016	26.645773699550784	24.977194993047217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	81.0
2	193.5
3	335.5
4	227.5
5	184.5
6	413.5
7	1098.5
8	2059.5
9	2484.0
10	1859.0
11	1229.0
12	1254.5
13	1430.0
14	1647.0
15	1973.0
16	2352.5
17	2736.5
18	3227.5
19	3622.5
20	4091.0
21	4761.0
22	5752.5
23	7142.5
24	8747.0
25	10925.0
26	14040.5
27	17976.0
28	22333.5
29	27522.5
30	33639.5
31	40052.5
32	49086.0
33	56579.5
34	61781.0
35	68469.0
36	75045.5
37	83796.0
38	90031.0
39	92903.5
40	95000.5
41	96009.0
42	98799.0
43	104424.5
44	110698.0
45	118883.0
46	127173.5
47	132012.0
48	135205.0
49	134301.5
50	131579.0
51	132026.5
52	131529.5
53	130005.0
54	127979.0
55	118028.5
56	107277.5
57	96782.5
58	85069.5
59	75976.0
60	64360.0
61	52445.0
62	42011.0
63	32417.0
64	24044.5
65	16775.0
66	12258.0
67	9592.0
68	7071.0
69	5153.0
70	3892.0
71	2899.5
72	2211.0
73	1657.5
74	1250.0
75	947.5
76	658.0
77	413.0
78	211.5
79	114.5
80	79.5
81	48.0
82	24.0
83	14.5
84	7.0
85	2.0
86	2.0
87	3.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002787277088256164
2	0.003498922302279014
3	2.9651883917618764E-4
4	0.051623929900574274
5	0.002016328106398076
6	0.00432917505197234
7	2.9651883917618764E-5
8	2.0756318742333135E-4
9	2.9651883917618764E-5
10-11	8.895565175285629E-5
12-13	0.0
14-15	4.4477825876428146E-5
16-17	2.0756318742333135E-4
18-19	1.1860753567047506E-4
20-21	2.2238912938214072E-4
22-23	0.0
24-25	0.0
26-27	2.2238912938214072E-4
28-29	1.4825941958809382E-5
30-31	2.0756318742333135E-4
32-33	1.4825941958809382E-4
34-35	1.4825941958809382E-4
36-37	4.4477825876428146E-5
38-39	6.671673881464222E-4
40-41	5.04082026599519E-4
42-43	0.0
44-45	4.5960420072309083E-4
46-47	0.0
48-49	1.1860753567047506E-4
50-51	0.0
52-53	4.744301426819002E-4
54-55	4.0030043288785336E-4
56-57	1.7791130350571258E-4
58-59	0.0010674678210342754
60-61	6.819933301052315E-4
62-63	2.372150713409501E-4
64-65	4.0030043288785336E-4
66-67	0.0013788126021692727
68-69	0.0028465808560914014
70-71	0.0037361373736199644
72-73	0.0034396185344437767
74-75	0.002994840275679495
76-77	0.004344000993931149
78-79	0.001467768253922129
80-81	0.00120090129866356
82-83	7.264711559816598E-4
84-85	4.151263748466627E-4
86-87	0.0
88-89	0.0
90-91	2.9651883917618764E-5
92-93	1.4825941958809382E-4
94-95	2.9651883917618764E-5
96-97	2.9651883917618764E-4
98-99	1.1860753567047506E-4
100	0.07688733499838545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3372467.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.41233396074022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.63894030592165	37.34804942637121
2	16.27262229066776	17.70862716993599
3	6.507341288860491	10.622388822179722
4	3.1909249606118038	6.9450269840188525
5	1.742859799003357	4.741653471505962
6	1.0303407915944793	3.3637948347367352
7	0.6393176979698971	2.4350737662264783
8	0.4712968822725238	2.0515490682294586
9	0.3181949247989187	1.5582355661494245
>10	1.1459429454659829	9.636455833426814
>50	0.027993823283689302	1.0258599717947419
>100	0.013104937611287741	1.2551529131454906
>500	7.749359572443821E-4	0.30219019774366285
>1k	1.7220799049874608E-4	0.2657868204340709
>5k	1.7220799049874608E-4	0.740155154101437
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8323	0.24679262984634098	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7475	0.22164783228420026	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4334	0.12851126489895973	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4825941958809382E-4
2	0.0	0.0	0.0	0.0	1.4825941958809382E-4
3	0.0	0.0	0.0	0.0	1.4825941958809382E-4
4	0.0	0.0	0.0	0.0	1.4825941958809382E-4
5	0.0	0.0	0.0	0.0	1.4825941958809382E-4
6	0.0	0.0	0.0	0.0	1.7791130350571258E-4
7	0.0	0.0	0.0	0.0	1.7791130350571258E-4
8	0.0	0.0	0.0	0.0	1.7791130350571258E-4
9	0.0	0.0	0.0	0.0	1.7791130350571258E-4
10-11	0.0	0.0	0.0	0.0	2.0756318742333135E-4
12-13	0.0	0.0	0.0	0.0	9.636862273226098E-4
14-15	0.0	0.0	0.0	0.0	0.001126771588869513
16-17	0.0	0.0	0.0	1.4825941958809382E-5	0.0014084644860868913
18-19	0.0	0.0	0.0	2.9651883917618764E-5	0.001512246079798557
20-21	0.0	0.0	0.0	7.412970979404691E-5	0.0016753314413454601
22-23	0.0	0.0	0.0	1.630853615469032E-4	0.0017642870930983164
24-25	0.0	0.0	0.0	4.1512637484666264E-4	0.0018087649189747446
26-27	0.0	0.0	0.0	9.488602853638005E-4	0.002090457816192123
28-29	0.0	0.0	0.0	0.004566390123313289	0.0021794134679449795
30-31	0.0	0.0	0.0	0.015685846592420328	0.0022090653518625977
32-33	0.0	0.0	0.0	0.040415517779714374	0.002253543177739026
34-35	0.0	0.0	0.0	0.0798080455642709	0.0023128469455742638
36-37	0.0	0.0	0.0	0.14323342526405744	0.0023128469455742638
38-39	0.0	0.0	0.0	0.23675546714022705	0.0023424988294918824
40-41	0.0	0.0	0.0	0.3386986440490003	0.0023424988294918824
42-43	1.4825941958809382E-5	0.0	0.0	0.4445855215188169	0.0024166285392859294
44-45	2.9651883917618764E-5	0.0	0.0	0.5800501531964583	0.002520410132997595
46-47	2.9651883917618764E-5	0.0	0.0	0.7454038838630592	0.002594539842791642
48-49	2.9651883917618764E-5	0.0	0.0	0.9194307905755639	0.0026983214365033075
50-51	2.9651883917618764E-5	0.0	0.0	1.1057632291138801	0.0028169289721737827
52-53	2.9651883917618764E-5	0.0	0.0	1.2924959680850843	0.002994840275679495
54-55	2.9651883917618764E-5	0.0	0.0	1.5030539957840952	0.0030837959274323513
56-57	2.9651883917618764E-5	0.0	0.0	1.756533125453859	0.003157925637226398
58-59	2.9651883917618764E-5	0.0	0.0	2.0389969716530953	0.0032024034631028264
60-61	2.9651883917618764E-5	0.0	0.0	2.336939101257329	0.0032617072309380642
62-63	2.9651883917618764E-5	0.0	0.0	2.6344068007188803	0.003498922302279014
64-65	2.9651883917618764E-5	0.0	0.0	2.952927337761941	0.0035582260701142515
66-67	2.9651883917618764E-5	0.0	0.0	3.310632839402135	0.003573052012073061
68-69	2.9651883917618764E-5	0.0	0.0	3.708709380996167	0.003632355779908299
70-71	2.9651883917618764E-5	0.0	0.0	4.144695856178874	0.003647181721867108
72-73	2.9651883917618764E-5	0.0	0.0	4.586746141622735	0.003721311431661155
74-75	2.9651883917618764E-5	0.0	0.0	5.030234543436601	0.003884396793208058
76-77	2.9651883917618764E-5	0.0	0.0	5.5222482532816475	0.003973352444960915
78-79	2.9651883917618764E-5	0.0	0.0	6.060904376529111	0.004047482154754961
80-81	2.9651883917618764E-5	0.0	0.0	6.631629012233478	0.004136437806507817
82-83	2.9651883917618764E-5	0.0	0.0	7.2321686172170105	0.004388478819807577
84-85	2.9651883917618764E-5	0.0	0.0	7.828586610335995	0.004403304761766386
86-87	2.9651883917618764E-5	0.0	0.0	8.449067107254127	0.004655345775066146
88	2.9651883917618764E-5	0.0	0.0	8.927796773104081	0.004892560846407096
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5010	0.0	60.266205	1
GTATCAA	9470	0.0	43.206177	1
ATCAACG	11780	0.0	34.21211	3
CAACGCA	11830	0.0	34.009598	5
TATCAAC	12345	0.0	32.838726	2
TCAACGC	12650	0.0	32.007885	4
AACGCAG	12930	0.0	31.260292	6
ACGCAGA	14430	0.0	27.878847	7
CGCAGAG	14790	0.0	27.104925	8
GTACATG	10340	0.0	25.834755	1
ACATGGG	10115	0.0	24.687265	3
TACATGG	10710	0.0	24.414928	2
GCAGAGT	16355	0.0	24.338861	9
AGAGTAC	15280	0.0	21.714445	10-11
GAGTACT	11075	0.0	21.66336	12-13
CATGGGG	8025	0.0	21.506283	4
CAGAGTA	16045	0.0	21.323526	10-11
GTGGTAT	1695	0.0	21.087275	1
GTACTTT	11860	0.0	20.090794	14-15
TAGTACT	1750	0.0	19.885569	4
>>END_MODULE