FastQCFastQC Report
Mon 16 Jan 2017
ERR1146601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5533306
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.038.401111
GTATCAA86000.036.0317961
CAACGCA92300.031.0657375
ATCAACG94100.030.573063
TATCAAC109000.028.1186372
AACGCAG111200.027.0469766
ACGCAGA115800.024.7954647
TCAACGC118200.024.7367234
GTACTAG27450.024.6671561
GTACATG189850.024.3467981
CGCAGAG118750.024.0996918
TACATGG193600.023.5526392
GTGATCG20400.023.496218
ACATGGG202300.021.7731293
TGATCGC21800.021.771529
TAGTACT33500.021.1885574
GTATAAG34000.021.0214941
TAGGCAT38350.020.591955
CATGGGG167350.019.8873424
GCAGAGT139950.019.6765339
Produced by FastQC (version 0.11.5)