##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146601_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5533306 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.787232623679227 31.0 31.0 34.0 27.0 34.0 2 31.005329182951385 31.0 31.0 34.0 27.0 34.0 3 30.99581678656485 31.0 31.0 34.0 27.0 34.0 4 34.523851021432755 37.0 35.0 37.0 30.0 37.0 5 34.610430545500286 37.0 35.0 37.0 30.0 37.0 6 34.61223145801082 37.0 35.0 37.0 30.0 37.0 7 34.56123843503323 37.0 35.0 37.0 30.0 37.0 8 34.54392419287854 37.0 35.0 37.0 30.0 37.0 9 36.05783721341274 39.0 37.0 39.0 30.0 39.0 10-11 36.06008315824211 39.0 37.0 39.0 30.0 39.0 12-13 36.01851587459649 39.0 37.0 39.0 30.0 39.0 14-15 37.25466981222438 40.0 38.0 41.0 30.5 41.0 16-17 37.24975891447175 40.0 37.5 41.0 30.5 41.0 18-19 37.22846793580547 40.0 37.0 41.0 30.0 41.0 20-21 37.34055047741802 40.0 37.5 41.0 30.0 41.0 22-23 37.330076720860916 40.0 37.0 41.0 30.0 41.0 24-25 37.33439882052429 40.0 37.0 41.0 30.0 41.0 26-27 37.38930993514546 40.0 37.0 41.0 30.0 41.0 28-29 37.36979059173666 40.0 37.0 41.0 30.0 41.0 30-31 37.33631494444732 40.0 37.0 41.0 30.0 41.0 32-33 37.31262061053555 40.0 37.0 41.0 30.0 41.0 34-35 37.25923001547357 40.0 37.0 41.0 30.0 41.0 36-37 37.1748562251934 40.0 37.0 41.0 30.0 41.0 38-39 37.106604622986694 40.0 37.0 41.0 30.0 41.0 40-41 37.04715363654206 40.0 36.5 41.0 30.0 41.0 42-43 36.869199624962 40.0 36.0 41.0 29.5 41.0 44-45 36.708700368278926 40.0 36.0 41.0 29.0 41.0 46-47 36.584903672415734 39.5 35.0 41.0 29.0 41.0 48-49 36.46547913670417 39.0 35.0 41.0 29.0 41.0 50-51 36.3199298032677 39.0 35.0 41.0 28.0 41.0 52-53 36.174943785866894 39.0 35.0 41.0 28.0 41.0 54-55 36.0486540053993 39.0 35.0 40.0 28.0 41.0 56-57 35.84921419491349 38.5 35.0 40.0 28.0 41.0 58-59 35.64114256468014 38.0 34.0 40.0 28.0 41.0 60-61 35.41995589978215 37.5 34.0 40.0 27.5 41.0 62-63 35.183275604132504 37.0 34.0 40.0 27.0 41.0 64-65 34.887011670780545 37.0 34.0 40.0 26.5 41.0 66-67 34.65276138713456 36.0 34.0 39.0 26.0 41.0 68-69 34.40419832194352 36.0 33.0 39.0 26.0 41.0 70-71 34.146992683939764 35.0 33.0 39.0 26.0 40.5 72-73 33.833342670728854 35.0 33.0 38.0 26.0 40.0 74-75 33.51985539928571 35.0 33.0 37.0 26.0 39.5 76-77 33.26994422502569 35.0 33.0 37.0 26.0 39.0 78-79 32.98348365335299 35.0 33.0 36.0 25.5 39.0 80-81 32.77161438387828 35.0 32.5 36.0 25.0 38.5 82-83 32.529259542125445 35.0 32.0 35.5 25.0 37.0 84-85 32.34891410306966 35.0 32.0 35.0 25.0 37.0 86-87 32.16309571529209 35.0 32.0 35.0 25.0 36.5 88-89 32.00525463439036 35.0 32.0 35.0 25.0 36.0 90-91 31.87203942814657 35.0 32.0 35.0 24.0 36.0 92-93 31.729892491035194 35.0 32.0 35.0 24.0 36.0 94-95 31.62800069253354 35.0 32.0 35.0 24.0 35.0 96-97 31.547935176547256 35.0 32.0 35.0 23.5 35.0 98-99 31.45174006281236 35.0 31.0 35.0 23.0 35.0 100 30.22360465877 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4838781723175316 1101 2 0.43816538787904236 1101 3 0.4445124470888011 1101 4 0.48799001522854013 1101 5 0.472655360605863 1101 6 0.516967302200726 1101 7 0.6231059529616374 1101 8 0.5989513701713065 1101 9 0.6830410675035097 1101 10-11 0.7368887740493903 1101 12-13 0.7368932926655205 1101 14-15 0.8510813050820403 1101 16-17 0.8535536726924775 1101 18-19 0.7809358336677263 1101 20-21 0.8160430286801557 1101 22-23 0.8913995398533814 1101 24-25 0.8476198080523147 1101 26-27 0.8401287576469656 1101 28-29 0.8190100985576834 1101 30-31 0.8608753356573118 1101 32-33 0.8815905718269477 1101 34-35 0.8404550657089445 1101 36-37 0.869710092833472 1101 38-39 0.9013265146430527 1101 40-41 0.874940750987939 1101 42-43 0.8610565538130146 1101 44-45 0.7343482671273946 1101 46-47 0.6905159961452583 1101 48-49 0.7040383067191556 1101 50-51 0.7381140619925333 1101 52-53 0.7286931873924942 1101 54-55 0.6990709152254908 1101 56-57 0.7383021858831711 1101 58-59 0.7791215336952604 1101 60-61 0.7312001012205513 1101 62-63 0.6863361268939343 1101 64-65 0.694767667686385 1101 66-67 0.6830462978382741 1101 68-69 0.6493314201312543 1101 70-71 0.6386880077582262 1101 72-73 0.6852426534317431 1101 74-75 0.6596147869284081 1101 76-77 0.584872980440359 1101 78-79 0.6540109736080879 1101 80-81 0.6243350472431572 1101 82-83 0.5919421789772272 1101 84-85 0.5718802344739089 1101 86-87 0.6342166798098816 1101 88-89 0.6423761979543414 1101 90-91 0.6077459500873523 1101 92-93 0.609568271573881 1101 94-95 0.6056451715733466 1101 96-97 0.5930096358248811 1101 98-99 0.5989325754584272 1101 100 0.609025539744426 1102 1 0.3423909422655491 1102 2 0.2744127687084479 1102 3 0.30823780214980445 1102 4 0.2905055960005072 1102 5 0.36595180110853676 1102 6 0.3844180840461888 1102 7 0.42331078780319586 1102 8 0.44697497049950385 1102 9 0.5463470978004139 1102 10-11 0.4911981815097164 1102 12-13 0.5253878476136506 1102 14-15 0.5594104399877011 1102 16-17 0.5800959592345052 1102 18-19 0.4875583088755491 1102 20-21 0.4568135666695454 1102 22-23 0.5430242371091154 1102 24-25 0.5257523461993046 1102 26-27 0.49104918571095624 1102 28-29 0.5128132377095582 1102 30-31 0.5338119689931062 1102 32-33 0.580236626029837 1102 34-35 0.5462133100216704 1102 36-37 0.5370877903136702 1102 38-39 0.5554732018054622 1102 40-41 0.5204093318372429 1102 42-43 0.5772006951604567 1102 44-45 0.5251488416139125 1102 46-47 0.5066419951358014 1102 48-49 0.5175663624994087 1102 50-51 0.5315069889376787 1102 52-53 0.4413072748176887 1102 54-55 0.4510197841235595 1102 56-57 0.4974512256669783 1102 58-59 0.49786163206491096 1102 60-61 0.4953025837441629 1102 62-63 0.49484382562560825 1102 64-65 0.5235100713215459 1102 66-67 0.4497172893681949 1102 68-69 0.38908570107921037 1102 70-71 0.39470424043206975 1102 72-73 0.4106998927792773 1102 74-75 0.4077228738127303 1102 76-77 0.4272088369375382 1102 78-79 0.3526889079688047 1102 80-81 0.30822650096088466 1102 82-83 0.37324539973753446 1102 84-85 0.4339710578895577 1102 86-87 0.3891516997356703 1102 88-89 0.3974618279479145 1102 90-91 0.3824401208000303 1102 92-93 0.4423247153519334 1102 94-95 0.4429443125561967 1102 96-97 0.41819159502103886 1102 98-99 0.4673370770065972 1102 100 0.3778138813353067 1103 1 0.16880717865067396 1103 2 0.17429424174352093 1103 3 0.18195446942570825 1103 4 0.23993076516413936 1103 5 0.17867809581763794 1103 6 0.19208973976490284 1103 7 0.21292429772194055 1103 8 0.22244719428057635 1103 9 0.3186241017601503 1103 10-11 0.32075077634802085 1103 12-13 0.30113650944735326 1103 14-15 0.36283449036813664 1103 16-17 0.38624673519883146 1103 18-19 0.4001144426570704 1103 20-21 0.3131457672004174 1103 22-23 0.3716801614237184 1103 24-25 0.4122592399209637 1103 26-27 0.3993651794551525 1103 28-29 0.37006205469902653 1103 30-31 0.5004758495609352 1103 32-33 0.48601384046489926 1103 34-35 0.44053058623533303 1103 36-37 0.4155330555526149 1103 38-39 0.4755284922565295 1103 40-41 0.3622183796648244 1103 42-43 0.4107617926658662 1103 44-45 0.3259841457659789 1103 46-47 0.3744590152656784 1103 48-49 0.2824330743886989 1103 50-51 0.31221285915148655 1103 52-53 0.32521079351672455 1103 54-55 0.28178210390733227 1103 56-57 0.3213793154143474 1103 58-59 0.3396192660718924 1103 60-61 0.24635110346019928 1103 62-63 0.26126486465809506 1103 64-65 0.23335072451708783 1103 66-67 0.23577334815051643 1103 68-69 0.2867092268220759 1103 70-71 0.2551357877322502 1103 72-73 0.3254482951826816 1103 74-75 0.2970670523612853 1103 76-77 0.22813218903270638 1103 78-79 0.2162378708538668 1103 80-81 0.20327037021163363 1103 82-83 0.20613427396606454 1103 84-85 0.21434778023257905 1103 86-87 0.2269831535500373 1103 88-89 0.1697139771601215 1103 90-91 0.2491303598276069 1103 92-93 0.21709051394820023 1103 94-95 0.17607254155405982 1103 96-97 0.16353463642938593 1103 98-99 0.21396778074728928 1103 100 0.2941899945239683 1104 1 0.20764954979387085 1104 2 0.2556090236095585 1104 3 0.18686983798460233 1104 4 0.27268161174882266 1104 5 0.2676168949283735 1104 6 0.2945951777211917 1104 7 0.33001665117077295 1104 8 0.3378627298168624 1104 9 0.41250972232906236 1104 10-11 0.37996748935788105 1104 12-13 0.5073725365982042 1104 14-15 0.5818933932700574 1104 16-17 0.5025943711553396 1104 18-19 0.47357582239880713 1104 20-21 0.4749872939126618 1104 22-23 0.5465305278871568 1104 24-25 0.5649877198117963 1104 26-27 0.5089332375663318 1104 28-29 0.5149355277258039 1104 30-31 0.513955739553495 1104 32-33 0.5219633472439114 1104 34-35 0.4658473998708246 1104 36-37 0.5345620089430412 1104 38-39 0.5439675095274126 1104 40-41 0.4821900416498792 1104 42-43 0.4900505230177288 1104 44-45 0.4651339766010736 1104 46-47 0.5376527463751444 1104 48-49 0.4853949425625359 1104 50-51 0.4447891016043499 1104 52-53 0.42556956265092083 1104 54-55 0.41349740347931174 1104 56-57 0.44880944462292405 1104 58-59 0.4569170431019174 1104 60-61 0.3972436090383624 1104 62-63 0.3919309515914051 1104 64-65 0.4187886149228248 1104 66-67 0.38511713627892163 1104 68-69 0.4151324450077354 1104 70-71 0.36036593021793095 1104 72-73 0.3939933435216574 1104 74-75 0.3815587713129105 1104 76-77 0.38743827760117 1104 78-79 0.40942578435940646 1104 80-81 0.3457465140659153 1104 82-83 0.30994497345860594 1104 84-85 0.3206137062189356 1104 86-87 0.349473944455319 1104 88-89 0.3469262418313761 1104 90-91 0.4017573694896619 1104 92-93 0.3610936436974903 1104 94-95 0.3375570727918422 1104 96-97 0.3164691057833302 1104 98-99 0.36096437515168134 1104 100 0.3944474285653641 1105 1 0.27674876917346225 1105 2 0.26262354146519584 1105 3 0.21944420140008702 1105 4 0.3058501608052211 1105 5 0.22068681397960432 1105 6 0.21644797845249286 1105 7 0.27312926979740837 1105 8 0.3055320546824518 1105 9 0.31886462195499377 1105 10-11 0.302585875434211 1105 12-13 0.36720306138199277 1105 14-15 0.4392852310357327 1105 16-17 0.3929902142614665 1105 18-19 0.3896952128172586 1105 20-21 0.39588927733800716 1105 22-23 0.4029264431615758 1105 24-25 0.44705474010311974 1105 26-27 0.44222964478797167 1105 28-29 0.4653489317840638 1105 30-31 0.45821835998433613 1105 32-33 0.43964758689076433 1105 34-35 0.44045955541756143 1105 36-37 0.4354114297924596 1105 38-39 0.4705618876171158 1105 40-41 0.43525875789615753 1105 42-43 0.4278935329168121 1105 44-45 0.35505459147495344 1105 46-47 0.37799614718583285 1105 48-49 0.3256243174648077 1105 50-51 0.29259879380656173 1105 52-53 0.37966379039001197 1105 54-55 0.31954919626006983 1105 56-57 0.38218980762945165 1105 58-59 0.3563194896071735 1105 60-61 0.38836510436546234 1105 62-63 0.38159524873233863 1105 64-65 0.39253463063145233 1105 66-67 0.36742435671698814 1105 68-69 0.33527100813694943 1105 70-71 0.3680887799033954 1105 72-73 0.29703343301035545 1105 74-75 0.33576257458213377 1105 76-77 0.36735083368195376 1105 78-79 0.34561746335482013 1105 80-81 0.370760286182481 1105 82-83 0.38500024234565444 1105 84-85 0.35950147248987463 1105 86-87 0.29312529941508814 1105 88-89 0.31436571067271046 1105 90-91 0.314627021518195 1105 92-93 0.3681182651146351 1105 94-95 0.4010188656928868 1105 96-97 0.4112193433373079 1105 98-99 0.44511621724745254 1105 100 0.49845325014127795 1106 1 0.12198654664540598 1106 2 0.1238856920965965 1106 3 0.1695550918739137 1106 4 0.18479122520727742 1106 5 0.19425724822384183 1106 6 0.18366499957160443 1106 7 0.27123383490744146 1106 8 0.25253884457959686 1106 9 0.3237903101100841 1106 10-11 0.29396371993003356 1106 12-13 0.29948331108120385 1106 14-15 0.32494044088337404 1106 16-17 0.3121130364963065 1106 18-19 0.2755447942168061 1106 20-21 0.25587669445928185 1106 22-23 0.3359868005014519 1106 24-25 0.3233457746863664 1106 26-27 0.31176114458308035 1106 28-29 0.29475918258967226 1106 30-31 0.3025836474302537 1106 32-33 0.2795954512130976 1106 34-35 0.2592376823342448 1106 36-37 0.29561272501169356 1106 38-39 0.27434017451832204 1106 40-41 0.2716351818065661 1106 42-43 0.2826085388092565 1106 44-45 0.2099054801214919 1106 46-47 0.25400072852563227 1106 48-49 0.22687542723693355 1106 50-51 0.24605386186551925 1106 52-53 0.22733333253745514 1106 54-55 0.20530287946991166 1106 56-57 0.16243320768982272 1106 58-59 0.19100602982052806 1106 60-61 0.15849221688392845 1106 62-63 0.08431294236469 1106 64-65 0.12819507178009104 1106 66-67 0.1258751977151178 1106 68-69 0.07796019753973127 1106 70-71 0.10553530363257124 1106 72-73 0.17628629374556226 1106 74-75 0.06917848305710805 1106 76-77 0.12309260093647367 1106 78-79 0.1705365902171465 1106 80-81 0.10707203779707442 1106 82-83 0.08188232527468386 1106 84-85 0.1511421619113591 1106 86-87 0.11784077947850591 1106 88-89 0.15981376562719873 1106 90-91 0.1195571738195973 1106 92-93 0.16065512998091336 1106 94-95 0.12233393072177279 1106 96-97 0.12990574322689952 1106 98-99 0.16283201612339582 1106 100 0.13606265738514622 1107 1 0.10782827244868898 1107 2 0.1332220413566887 1107 3 0.1561662991226136 1107 4 0.1832078375364432 1107 5 0.17774770303509513 1107 6 0.18553283798704712 1107 7 0.17774140134181238 1107 8 0.20834851247202124 1107 9 0.35231827189794984 1107 10-11 0.29534164107252536 1107 12-13 0.33021415649060515 1107 14-15 0.33010106058861766 1107 16-17 0.3111841498432213 1107 18-19 0.26384462715289203 1107 20-21 0.3474637413735877 1107 22-23 0.33168347455754343 1107 24-25 0.37377818791556194 1107 26-27 0.2854671991058808 1107 28-29 0.29820403154565156 1107 30-31 0.2818440825629054 1107 32-33 0.312217976285325 1107 34-35 0.3027808048658187 1107 36-37 0.3330760682294809 1107 38-39 0.32529505143486404 1107 40-41 0.25351020962967397 1107 42-43 0.29542438866106124 1107 44-45 0.26071397500610516 1107 46-47 0.3019371022216504 1107 48-49 0.3069983531079572 1107 50-51 0.39534748404526 1107 52-53 0.30106063442909203 1107 54-55 0.2518275716492866 1107 56-57 0.3043397465272477 1107 58-59 0.3061581790064096 1107 60-61 0.34148178114813277 1107 62-63 0.28831707179473653 1107 64-65 0.2988969816138294 1107 66-67 0.2717482885502136 1107 68-69 0.23631231818065146 1107 70-71 0.19950597587108376 1107 72-73 0.245018182023216 1107 74-75 0.348039611486783 1107 76-77 0.31293938584784087 1107 78-79 0.28366362405111545 1107 80-81 0.1970135622781939 1107 82-83 0.26069899511788464 1107 84-85 0.23592941308437076 1107 86-87 0.21253581688636558 1107 88-89 0.26162547300272365 1107 90-91 0.24757999312801005 1107 92-93 0.29702615068426397 1107 94-95 0.28062566070707007 1107 96-97 0.2254317360796243 1107 98-99 0.28184547160709883 1107 100 0.35133550643940836 1108 1 0.1707734642327985 1108 2 0.1288985937383771 1108 3 0.17349061035841018 1108 4 0.14966623709999993 1108 5 0.22803247536990057 1108 6 0.2126163680858184 1108 7 0.17621773574902022 1108 8 0.16457432844731557 1108 9 0.1828757853239722 1108 10-11 0.20353709117374308 1108 12-13 0.2535705171339586 1108 14-15 0.2397203540572761 1108 16-17 0.22820897536411877 1108 18-19 0.22280582253693382 1108 20-21 0.2478480584056939 1108 22-23 0.2999463483037985 1108 24-25 0.2525758681677246 1108 26-27 0.2472469956996406 1108 28-29 0.22032712052157422 1108 30-31 0.22824138553509243 1108 32-33 0.2776231019896471 1108 34-35 0.2239159598971625 1108 36-37 0.30702522072692773 1108 38-39 0.29753963252399984 1108 40-41 0.28045630110259623 1108 42-43 0.30342465819278175 1108 44-45 0.33663453297602075 1108 46-47 0.3812917512418892 1108 48-49 0.3088952536498297 1108 50-51 0.24107057253495157 1108 52-53 0.29128151995386986 1108 54-55 0.27983570950389947 1108 56-57 0.2945714475909682 1108 58-59 0.2823263403239338 1108 60-61 0.29414772508978615 1108 62-63 0.24846887714690524 1108 64-65 0.30716383916902856 1108 66-67 0.32762166294431694 1108 68-69 0.27433008721045127 1108 70-71 0.31274118300007814 1108 72-73 0.28117078683190755 1108 74-75 0.3209133171717582 1108 76-77 0.26276207325165046 1108 78-79 0.2853211108857039 1108 80-81 0.22717952829760435 1108 82-83 0.28715318094161546 1108 84-85 0.2738861259437684 1108 86-87 0.2436874248641061 1108 88-89 0.2904765272439036 1108 90-91 0.28919404967902906 1108 92-93 0.2734340112920144 1108 94-95 0.28859395959355894 1108 96-97 0.20532406017667526 1108 98-99 0.25309572608713893 1108 100 0.3601974236702361 1109 1 0.05603605098765385 1109 2 0.04478560868902548 1109 3 0.009999716349685883 1109 4 0.055286798602871556 1109 5 -0.03721916225973132 1109 6 0.058190723107323095 1109 7 0.030262498912826175 1109 8 0.0827522290310938 1109 9 0.11721883705335046 1109 10-11 0.0956327076786323 1109 12-13 0.1247843329933076 1109 14-15 0.09589119603872831 1109 16-17 0.1271109419541574 1109 18-19 0.024442651641962243 1109 20-21 0.06686012941220554 1109 22-23 0.11876280285084562 1109 24-25 0.18406022408915845 1109 26-27 0.06339590363749181 1109 28-29 0.14649797613195403 1109 30-31 0.10659754592822424 1109 32-33 0.01860782226471258 1109 34-35 0.057161425630980034 1109 36-37 0.07087386316081279 1109 38-39 0.09609784443468783 1109 40-41 0.13246642318573976 1109 42-43 0.15953605600856946 1109 44-45 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-0.8917953706235338 2215 22-23 -0.8471499234312958 2215 24-25 -0.8372698700581154 2215 26-27 -0.8725073511271475 2215 28-29 -0.9241635002050614 2215 30-31 -0.881795612102863 2215 32-33 -0.900668806132785 2215 34-35 -0.9178897941359452 2215 36-37 -0.8515238257949136 2215 38-39 -0.9516535280063323 2215 40-41 -0.9923727026176223 2215 42-43 -0.9769858816951853 2215 44-45 -0.8610576306560063 2215 46-47 -0.8173985335156715 2215 48-49 -0.861277955137318 2215 50-51 -0.8460059416297128 2215 52-53 -0.7586811355920702 2215 54-55 -0.8364180016350673 2215 56-57 -0.9222459701423773 2215 58-59 -0.8340375122942305 2215 60-61 -0.787455709614747 2215 62-63 -0.8274377887015945 2215 64-65 -0.8097869630109003 2215 66-67 -0.804695613739213 2215 68-69 -0.8554176816399561 2215 70-71 -0.7746363554262032 2215 72-73 -0.6108587352071808 2215 74-75 -0.622688707838293 2215 76-77 -0.716730153974197 2215 78-79 -0.6572736451128094 2215 80-81 -0.7009304440359543 2215 82-83 -0.7113499605449256 2215 84-85 -0.6713475302224055 2215 86-87 -0.6853477009568287 2215 88-89 -0.6636118866143299 2215 90-91 -0.6874123310407576 2215 92-93 -0.6905970657595866 2215 94-95 -0.6659896279949145 2215 96-97 -0.6658069153887105 2215 98-99 -0.6782463614916594 2215 100 -0.6806640227976928 2216 1 -0.43536108490097547 2216 2 -0.4527586523370637 2216 3 -0.35692044854252813 2216 4 -0.3212287045825448 2216 5 -0.46869767528093575 2216 6 -0.37904242981115743 2216 7 -0.45599025799403137 2216 8 -0.43624659755562334 2216 9 -0.429130459205858 2216 10-11 -0.647494137682699 2216 12-13 -0.5937844775588204 2216 14-15 -0.827989896034282 2216 16-17 -0.7579956850662981 2216 18-19 -0.6939599765580482 2216 20-21 -0.6989941461740372 2216 22-23 -0.7645639651020772 2216 24-25 -0.7411734940336245 2216 26-27 -0.8323784876927647 2216 28-29 -0.9162896974994226 2216 30-31 -0.8774791314076964 2216 32-33 -0.9015599881215337 2216 34-35 -0.8239475605030862 2216 36-37 -0.7146236579268361 2216 38-39 -0.7160824803120462 2216 40-41 -0.8109745590410355 2216 42-43 -0.7369003677226402 2216 44-45 -0.6812136492202399 2216 46-47 -0.676075831827113 2216 48-49 -0.6012007358226157 2216 50-51 -0.7037304404941409 2216 52-53 -0.676360803805764 2216 54-55 -0.6718767752106629 2216 56-57 -0.6395175214408866 2216 58-59 -0.6684166966528693 2216 60-61 -0.6584932330669062 2216 62-63 -0.7042581698608714 2216 64-65 -0.7127431198194287 2216 66-67 -0.6044105342487427 2216 68-69 -0.643737025966999 2216 70-71 -0.6776801986176721 2216 72-73 -0.5743002282455905 2216 74-75 -0.507303993707751 2216 76-77 -0.6192063068328864 2216 78-79 -0.4820951124772819 2216 80-81 -0.5484144965687889 2216 82-83 -0.5884601609991584 2216 84-85 -0.5932919362656541 2216 86-87 -0.6011358909439899 2216 88-89 -0.5182011035591287 2216 90-91 -0.534674995205858 2216 92-93 -0.4515793902387024 2216 94-95 -0.5432877419477116 2216 96-97 -0.5722476184597092 2216 98-99 -0.497949531235907 2216 100 -0.5682321081141737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1761.0 1 418.0 2 295.0 3 284.0 4 238.0 5 359.0 6 543.0 7 1343.0 8 4100.0 9 5960.0 10 6791.0 11 9276.0 12 12996.0 13 17083.0 14 19803.0 15 20944.0 16 22621.0 17 24289.0 18 26659.0 19 28593.0 20 30661.0 21 33547.0 22 37635.0 23 42975.0 24 51060.0 25 60508.0 26 64250.0 27 63288.0 28 67778.0 29 80862.0 30 92979.0 31 108173.0 32 136030.0 33 180182.0 34 267794.0 35 406752.0 36 744143.0 37 1313105.0 38 1239735.0 39 307123.0 40 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86490758565697 20.239640176134824 13.242311286572198 24.653140951636004 2 15.01212624167163 20.458238294257075 40.25197916069517 24.277656303376123 3 17.67784099212441 26.499516005110525 30.18418935558504 25.638453647180025 4 12.616268922214877 17.502245027704504 39.370397467120405 30.511088582960216 5 13.407427557726646 38.330177745108536 35.16107246108389 13.101322236080925 6 29.905530314842277 38.664108067473805 18.24553870041056 13.184822917273367 7 26.373534942786826 32.89125906246829 23.224336820765934 17.510869173978953 8 25.785130125779272 35.06604053775892 20.806016162576462 18.342813173885343 9 26.53416727794633 15.480096878784327 20.85329939103524 37.1324364522341 10-11 24.635372910677418 26.42772976331184 28.513909826451595 20.42298749955914 12-13 25.079996299829215 24.41829689240813 27.91830399073185 22.583402817030805 14-15 22.777857658962805 25.478767128862216 25.338833379001258 26.404541833173724 16-17 22.433108313477888 28.121588675689708 26.313586226024814 23.131716784807594 18-19 22.38042093552542 28.170665062503318 27.433933390384585 22.014980611586672 20-21 23.610391719554002 27.2175516026798 26.614272455204613 22.557784222561583 22-23 23.75779398249525 26.99029540607744 26.343762566837377 22.908148044589932 24-25 23.35274291899791 27.230286188146074 26.698974779596153 22.71799611325986 26-27 23.05898300057602 27.465377655962186 26.41615899285715 23.05948035060464 28-29 22.861629449939304 27.561934856837862 26.554031500382493 23.022404192840344 30-31 23.198544959141863 27.265375475568867 26.460304356838442 23.07577520845083 32-33 23.315645683625007 27.62487591633795 25.99032690075912 23.069151499277925 34-35 23.415385828697282 27.409625042417247 26.499481225350063 22.67550790353541 36-37 23.382803664881692 27.21919178983149 26.49362451531773 22.904380029969083 38-39 23.37029580073533 27.189812066732323 26.651397260135205 22.788494872397138 40-41 23.21868809589939 26.769631437178575 26.939922967705023 23.071757499217004 42-43 23.172148506447197 27.023671001880817 26.532387934359924 23.27179255731206 44-45 23.027608167624276 27.4039661643119 26.57032894360791 22.998096724455916 46-47 23.034100465919387 27.08051857893909 26.53986867941333 23.345512275728193 48-49 23.324319283680627 26.830165934128154 26.644213850232912 23.201300931958308 50-51 23.11889454905394 26.976014187605724 26.381834032761624 23.52325723057871 52-53 22.856847990410863 27.263025285636317 26.41499399141196 23.46513273254086 54-55 22.94220177644774 27.14756302800698 26.356357500701836 23.55387769484344 56-57 23.29947530610159 26.87330012653797 26.339100051827202 23.488124515533237 58-59 22.906960778336792 26.912089774804997 26.845245665218286 23.33570378163992 60-61 22.92585971894801 27.144922920595455 26.545538550013713 23.383678810442817 62-63 23.13352245668722 26.85276952226167 26.387836503424385 23.625871517626724 64-65 22.665291162460857 26.991064951417144 26.462840582017378 23.88080330410462 66-67 23.11114174541734 26.753446178539146 26.38540031681179 23.75001175923172 68-69 22.665709913726705 26.637986390240687 26.667555519454705 24.028748176577903 70-71 22.72194987283863 26.52382887252421 26.695557330077925 24.05866392455924 72-73 23.023236061659997 26.614569666531622 26.708613091492396 23.65358118031599 74-75 22.839266128904598 26.89976323091431 26.668177285093048 23.592793355088048 76-77 22.836074691777515 26.735225577855253 26.57118819777508 23.857511532592152 78-79 22.631165451265055 26.708275118739678 26.698858262748377 23.96170116724689 80-81 22.56592248512751 26.660713479689953 26.971265771957903 23.802098263224636 82-83 22.877022514374744 26.211636135746957 27.373610604041467 23.537730745836832 84-85 22.994086647934527 26.524694235293573 26.769032824608185 23.712186292163718 86-87 22.33884760402032 26.800363187847136 27.029354608900093 23.831434599232452 88-89 22.816675915385755 26.167480056460153 26.93436846625484 24.081475561899257 90-91 22.73325201696699 26.100228979289653 27.119686565658895 24.04683243808446 92-93 22.898301115787348 26.15602065060868 27.00469847566135 23.940979757942625 94-95 22.258545870997864 26.043649159138283 27.428253105644835 24.269551864219014 96-97 22.364390861977725 26.111711520064528 27.201483165380118 24.322414452577625 98-99 22.226666681139804 26.42493453619569 27.04397770992289 24.304421072741622 100 22.76498266917214 25.979139234341925 26.67519861788188 24.580679478604058 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2149.0 1 1405.5 2 670.0 3 688.0 4 653.0 5 564.0 6 504.0 7 502.0 8 558.5 9 561.5 10 527.5 11 519.0 12 558.5 13 613.5 14 652.5 15 750.5 16 863.0 17 980.0 18 1228.5 19 1564.0 20 2059.0 21 2944.5 22 4532.5 23 6612.5 24 9020.0 25 12374.5 26 17647.5 27 23887.5 28 30299.5 29 39922.0 30 52596.0 31 64480.0 32 78613.5 33 93521.0 34 105967.0 35 119170.5 36 134588.5 37 153162.5 38 161981.5 39 161292.0 40 161744.0 41 160728.0 42 163416.5 43 174719.0 44 186418.0 45 202182.5 46 216136.5 47 223991.0 48 229901.5 49 225194.0 50 217316.0 51 219050.5 52 219316.5 53 214815.0 54 209956.0 55 193667.0 56 176064.0 57 156521.5 58 137171.5 59 123275.5 60 101466.0 61 79646.0 62 63515.0 63 49749.5 64 36530.0 65 24851.0 66 18658.0 67 14429.0 68 10641.5 69 7911.5 70 5970.5 71 4434.5 72 3299.5 73 2648.0 74 2251.0 75 1735.0 76 1217.0 77 817.5 78 475.5 79 284.5 80 195.0 81 163.0 82 123.0 83 87.5 84 68.5 85 57.0 86 48.0 87 37.0 88 32.5 89 34.5 90 29.0 91 23.0 92 21.0 93 18.5 94 18.5 95 16.5 96 18.5 97 23.5 98 20.5 99 14.5 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09520528956829787 2 0.0643918843454528 3 0.07810881957368705 4 0.07989798503823935 5 0.081144979149897 6 0.08602452132594872 7 0.08307872364188787 8 0.08551849472991373 9 0.0708798682017586 10-11 0.07772929962666081 12-13 0.06924431795385977 14-15 0.07700640448946797 16-17 0.07927448798241052 18-19 0.07878653376480534 20-21 0.07124131577035502 22-23 0.07446723532007808 24-25 0.07682568070516975 26-27 0.07230758609771445 28-29 0.0804672649587787 30-31 0.08428957299668588 32-33 0.0863498241376855 34-35 0.08844622003554475 36-37 0.08931369420017618 38-39 0.09109382347551355 40-41 0.08756970968169843 42-43 0.09268419277733782 44-45 0.08931369420017618 46-47 0.08628657081318113 48-49 0.09522336194672769 50-51 0.09217816618130283 52-53 0.09731975784458693 54-55 0.08981972079621116 56-57 0.09616312562507838 58-59 0.09974145655418298 60-61 0.09881072906504718 62-63 0.09976856512182772 64-65 0.10185592483047205 66-67 0.10362701791659452 68-69 0.10087098020604679 70-71 0.10272339899510348 72-73 0.10013001269042414 74-75 0.10425051497242337 76-77 0.10125050015307305 78-79 0.10813607633483491 80-81 0.10363605410580945 82-83 0.10669932224966411 84-85 0.10356376459209014 86-87 0.10098845066584064 88-89 0.09964205847281897 90-91 0.09964205847281897 92-93 0.09463600964775849 94-95 0.0944643220526752 96-97 0.09270226515576764 98-99 0.10525353197527844 100 0.24690483410821665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5533306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.998243813402254 #Duplication Level Percentage of deduplicated Percentage of total 1 68.3593130393673 33.494862872196066 2 14.15612571217032 13.87250598196188 3 6.457138719903694 9.49165372004404 4 3.4528147089633623 6.767274278091535 5 2.1249815915763155 5.206018306152394 6 1.3104889982798678 3.852699567149895 7 0.8997604416048685 3.086067704398688 8 0.6351802858978846 2.4898174811112876 9 0.4775636757114032 2.105980327703871 >10 2.0521063386969725 16.278726841171746 >50 0.05717343014531277 1.9052041893816627 >100 0.017203558355007875 1.378207498911018 >500 7.474966389814073E-5 0.019887930253614777 >1k 7.47496638863855E-5 0.051093301472246055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 1.807237842982116E-5 0.0 0.0 0.0 16-17 0.0 1.807237842982116E-5 0.0 0.0 1.807237842982116E-5 18-19 0.0 1.807237842982116E-5 0.0 1.807237842982116E-5 1.807237842982116E-5 20-21 0.0 1.807237842982116E-5 0.0 3.614475685964232E-5 2.710856764473174E-5 22-23 0.0 1.807237842982116E-5 0.0 1.0843427057892697E-4 4.51809460745529E-5 24-25 0.0 1.807237842982116E-5 0.0 2.8915805487713856E-4 5.4217135289463476E-5 26-27 0.0 1.807237842982116E-5 0.0 8.494017862015946E-4 5.4217135289463476E-5 28-29 0.0 1.807237842982116E-5 0.0 0.003542186172244947 5.4217135289463476E-5 30-31 0.0 1.807237842982116E-5 0.0 0.012831388685173023 5.4217135289463476E-5 32-33 0.0 1.807237842982116E-5 0.0 0.03453631517938824 5.4217135289463476E-5 34-35 0.0 1.807237842982116E-5 0.0 0.06928949889993433 5.4217135289463476E-5 36-37 0.0 1.807237842982116E-5 0.0 0.12577471768234036 5.4217135289463476E-5 38-39 0.0 1.807237842982116E-5 0.0 0.21002814592216662 5.4217135289463476E-5 40-41 0.0 1.807237842982116E-5 0.0 0.3027665558347939 5.4217135289463476E-5 42-43 0.0 1.807237842982116E-5 0.0 0.400980896411657 5.4217135289463476E-5 44-45 0.0 1.807237842982116E-5 0.0 0.5262947684440369 5.4217135289463476E-5 46-47 0.0 1.807237842982116E-5 0.0 0.6778406977673023 5.4217135289463476E-5 48-49 0.0 1.807237842982116E-5 0.0 0.8429047661560738 5.4217135289463476E-5 50-51 0.0 1.807237842982116E-5 0.0 1.0180532217086855 5.4217135289463476E-5 52-53 0.0 1.807237842982116E-5 0.0 1.1934637267485297 5.4217135289463476E-5 54-55 0.0 1.807237842982116E-5 0.0 1.3869917911642697 5.4217135289463476E-5 56-57 9.03618921491058E-6 1.807237842982116E-5 0.0 1.6183091988767655 5.4217135289463476E-5 58-59 1.807237842982116E-5 1.807237842982116E-5 0.0 1.8792291624573085 5.4217135289463476E-5 60-61 1.807237842982116E-5 1.807237842982116E-5 0.0 2.1523028005319063 5.4217135289463476E-5 62-63 1.807237842982116E-5 1.807237842982116E-5 0.0 2.432469847140209 5.4217135289463476E-5 64-65 1.807237842982116E-5 1.807237842982116E-5 0.0 2.7279532344677846 5.4217135289463476E-5 66-67 1.807237842982116E-5 1.807237842982116E-5 0.0 3.066367556755401 5.4217135289463476E-5 68-69 1.807237842982116E-5 1.807237842982116E-5 0.0 3.443294117476966 5.4217135289463476E-5 70-71 1.807237842982116E-5 1.807237842982116E-5 0.0 3.847880453385372 5.4217135289463476E-5 72-73 1.807237842982116E-5 1.807237842982116E-5 0.0 4.262686719295842 5.4217135289463476E-5 74-75 1.807237842982116E-5 1.807237842982116E-5 0.0 4.685209890795846 5.4217135289463476E-5 76-77 1.807237842982116E-5 1.807237842982116E-5 0.0 5.15627547075835 5.4217135289463476E-5 78-79 1.807237842982116E-5 1.807237842982116E-5 0.0 5.667091247077245 5.4217135289463476E-5 80-81 1.807237842982116E-5 1.807237842982116E-5 0.0 6.218343970132865 5.4217135289463476E-5 82-83 1.807237842982116E-5 3.614475685964232E-5 0.0 6.784343753987219 5.4217135289463476E-5 84-85 2.710856764473174E-5 4.51809460745529E-5 0.0 7.34855437237702 5.4217135289463476E-5 86-87 3.614475685964232E-5 5.4217135289463476E-5 0.0 7.947942875380469 5.4217135289463476E-5 88 3.614475685964232E-5 5.4217135289463476E-5 0.0 8.413957225571837 5.4217135289463476E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5400 0.0 38.40111 1 GTATCAA 8600 0.0 36.031796 1 CAACGCA 9230 0.0 31.065737 5 ATCAACG 9410 0.0 30.57306 3 TATCAAC 10900 0.0 28.118637 2 AACGCAG 11120 0.0 27.046976 6 ACGCAGA 11580 0.0 24.795464 7 TCAACGC 11820 0.0 24.736723 4 GTACTAG 2745 0.0 24.667156 1 GTACATG 18985 0.0 24.346798 1 CGCAGAG 11875 0.0 24.099691 8 TACATGG 19360 0.0 23.552639 2 GTGATCG 2040 0.0 23.49621 8 ACATGGG 20230 0.0 21.773129 3 TGATCGC 2180 0.0 21.77152 9 TAGTACT 3350 0.0 21.188557 4 GTATAAG 3400 0.0 21.021494 1 TAGGCAT 3835 0.0 20.59195 5 CATGGGG 16735 0.0 19.887342 4 GCAGAGT 13995 0.0 19.676533 9 >>END_MODULE