FastQCFastQC Report
Mon 16 Jan 2017
ERR1146601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5533306
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133520.24130239679497212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123640.22344688690630882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65050.11756082168598665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97200.056.117991
GTATCAA178300.043.7000121
CAACGCA224750.033.8376965
ATCAACG224800.033.7814873
TATCAAC235250.032.781182
AACGCAG247950.031.3147016
TCAACGC249600.030.5757054
ACGCAGA263750.028.867247
GTGGTAT30050.028.7927881
CGCAGAG268450.028.2918058
GCAGAGT295250.025.4212999
GTACATG187250.023.9572491
TACATGG194900.022.606752
GAGTACT189750.022.53919412-13
AGAGTAC273600.022.50286910-11
CAGAGTA289600.021.79516410-11
ACATGGG198050.021.4157943
AGTACTT203000.020.54712712-13
TGGTATC40200.020.1192972
GTACTTT211600.020.10081514-15
Produced by FastQC (version 0.11.5)