##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146601_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5533306
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27304237285991	33.0	31.0	34.0	30.0	34.0
2	32.33597075600012	34.0	31.0	34.0	30.0	34.0
3	32.459110701631175	34.0	31.0	34.0	30.0	34.0
4	36.00184284042849	37.0	35.0	37.0	35.0	37.0
5	36.05331116695878	37.0	35.0	37.0	35.0	37.0
6	36.12618224981593	37.0	36.0	37.0	35.0	37.0
7	36.08928297115685	37.0	36.0	37.0	35.0	37.0
8	36.09134846328759	37.0	36.0	37.0	35.0	37.0
9	37.873601423814264	39.0	38.0	39.0	35.0	39.0
10-11	37.82194469273884	39.0	38.0	39.0	35.0	39.0
12-13	37.802462216982036	39.0	38.0	39.0	35.0	39.0
14-15	39.15595712581231	40.0	39.0	41.0	36.0	41.0
16-17	39.097169576379834	40.0	39.0	41.0	36.0	41.0
18-19	39.189597322107254	40.0	39.0	41.0	36.0	41.0
20-21	39.24559530956719	40.5	39.0	41.0	36.0	41.0
22-23	39.216078687858584	40.0	39.0	41.0	36.0	41.0
24-25	39.2035235354777	41.0	39.0	41.0	36.0	41.0
26-27	39.12678315639872	40.5	39.0	41.0	35.0	41.0
28-29	39.06843910313292	40.0	39.0	41.0	35.0	41.0
30-31	38.93284385501181	40.0	38.5	41.0	35.0	41.0
32-33	38.837656637821944	40.0	38.0	41.0	35.0	41.0
34-35	38.77213721778625	40.0	38.0	41.0	35.0	41.0
36-37	38.70871582016248	40.0	38.0	41.0	35.0	41.0
38-39	38.6154554799608	40.0	38.0	41.0	35.0	41.0
40-41	38.512322289784805	40.0	38.0	41.0	34.5	41.0
42-43	38.4070149021218	40.0	38.0	41.0	34.0	41.0
44-45	38.25221115550089	40.0	38.0	41.0	34.0	41.0
46-47	38.00972790949931	40.0	37.5	41.0	33.0	41.0
48-49	37.71809981230028	40.0	37.0	41.0	33.0	41.0
50-51	37.597310721655376	40.0	37.0	41.0	33.0	41.0
52-53	37.47508921429612	40.0	36.5	41.0	33.0	41.0
54-55	37.29942334654906	40.0	36.0	41.0	32.5	41.0
56-57	37.12223433874794	39.0	35.5	41.0	32.0	41.0
58-59	36.91724892857904	39.0	35.0	41.0	32.0	41.0
60-61	36.70087828144693	39.0	35.0	41.0	32.0	41.0
62-63	36.44644964511271	38.0	35.0	41.0	31.0	41.0
64-65	36.157654845042	37.0	35.0	40.0	31.0	41.0
66-67	35.881802940231395	37.0	35.0	40.0	31.0	41.0
68-69	35.5879505850571	36.5	35.0	39.5	31.0	41.0
70-71	35.29751562628201	36.0	35.0	39.0	30.5	41.0
72-73	34.99261725268764	35.5	35.0	39.0	30.0	41.0
74-75	34.68906662671466	35.0	34.5	37.5	30.0	40.0
76-77	34.41553494420876	35.0	34.0	37.0	30.0	39.0
78-79	34.15213273945088	35.0	34.0	37.0	30.0	39.0
80-81	33.921301930527605	35.0	34.0	36.0	30.0	39.0
82-83	33.686101401223794	35.0	34.0	36.0	29.0	37.0
84-85	33.49777032031122	35.0	34.0	35.5	29.0	37.0
86-87	33.329899521190406	35.0	34.0	35.0	29.0	37.0
88-89	33.1545922094314	35.0	34.0	35.0	29.0	36.0
90-91	33.08198082665228	35.0	34.0	35.0	29.0	36.0
92-93	32.992751891906934	35.0	34.0	35.0	29.0	36.0
94-95	32.93276189677563	35.0	34.0	35.0	29.0	36.0
96-97	32.882511106380164	35.0	34.0	35.0	29.0	35.0
98-99	32.82036272709299	35.0	34.0	35.0	29.0	35.0
100	32.0794103922682	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35385583887553196
1101	2	0.3420096627306748
1101	3	0.34002743677507397
1101	4	0.24816891331800406
1101	5	0.23240217605999902
1101	6	0.2811708206210781
1101	7	0.2896301551684317
1101	8	0.2728116970101553
1101	9	0.3071951942123121
1101	10-11	0.3586137546376733
1101	12-13	0.3482608245695644
1101	14-15	0.47819472000332297
1101	16-17	0.5296296341151745
1101	18-19	0.5038853748530627
1101	20-21	0.4632699907312201
1101	22-23	0.42971973278761055
1101	24-25	0.46548261608092645
1101	26-27	0.43978212853816956
1101	28-29	0.4646801369078801
1101	30-31	0.5244699724232404
1101	32-33	0.5140743739084499
1101	34-35	0.5110971781740403
1101	36-37	0.5276374099872712
1101	38-39	0.5312137522618912
1101	40-41	0.535156894658094
1101	42-43	0.5424651138437397
1101	44-45	0.5341266021549558
1101	46-47	0.5086013298181911
1101	48-49	0.6301662026302566
1101	50-51	0.5712856610490249
1101	52-53	0.5211570025598959
1101	54-55	0.5605410803700934
1101	56-57	0.5183249306466777
1101	58-59	0.548764864307195
1101	60-61	0.4922507029481267
1101	62-63	0.4569830908021473
1101	64-65	0.4418436666651857
1101	66-67	0.42513353950459987
1101	68-69	0.35635735081055486
1101	70-71	0.39190280401416544
1101	72-73	0.42439296240084445
1101	74-75	0.42048875333013314
1101	76-77	0.3766186974986496
1101	78-79	0.35866109769488475
1101	80-81	0.3234032263065103
1101	82-83	0.34103946565858934
1101	84-85	0.31962454023936715
1101	86-87	0.3187415187274283
1101	88-89	0.3497113503269418
1101	90-91	0.3528196922180271
1101	92-93	0.3681290942162221
1101	94-95	0.41218119196070546
1101	96-97	0.3607927436522971
1101	98-99	0.4102933365846084
1101	100	0.4891654116384174
1102	1	0.2677511279592295
1102	2	0.252666398579521
1102	3	0.25967007372297246
1102	4	0.17294413613657866
1102	5	0.16011932963546371
1102	6	0.1985158793299533
1102	7	0.1618109335941611
1102	8	0.18739341637409979
1102	9	0.26924875821074323
1102	10-11	0.2449081097348227
1102	12-13	0.2592626697934648
1102	14-15	0.40889846598164326
1102	16-17	0.4757082597612978
1102	18-19	0.4045279434797706
1102	20-21	0.36659809714088
1102	22-23	0.3761912558619187
1102	24-25	0.3612315666885735
1102	26-27	0.3581560510656203
1102	28-29	0.38774401041785467
1102	30-31	0.3821095736297764
1102	32-33	0.4075508586828889
1102	34-35	0.4230413501050947
1102	36-37	0.4200300521535141
1102	38-39	0.41262532142128094
1102	40-41	0.4144030590392518
1102	42-43	0.4352829014085984
1102	44-45	0.4148043851607781
1102	46-47	0.46053214831250955
1102	48-49	0.5447867078337438
1102	50-51	0.529139274021702
1102	52-53	0.4337204789701872
1102	54-55	0.4288125073165858
1102	56-57	0.43296455291048375
1102	58-59	0.4258884832742993
1102	60-61	0.4113677080928113
1102	62-63	0.4007210673527979
1102	64-65	0.41723365607494856
1102	66-67	0.37478730370343527
1102	68-69	0.3975522617920859
1102	70-71	0.3985699684538915
1102	72-73	0.36345791654426307
1102	74-75	0.386970790327851
1102	76-77	0.3536637424973108
1102	78-79	0.3548161134220891
1102	80-81	0.34279882191603406
1102	82-83	0.3117809381761347
1102	84-85	0.299963457489163
1102	86-87	0.2889679800308329
1102	88-89	0.40048869928630637
1102	90-91	0.283440680359476
1102	92-93	0.2781473533536314
1102	94-95	0.3169208528172547
1102	96-97	0.291363663302441
1102	98-99	0.29589572582175094
1102	100	0.39441287458198815
1103	1	0.19206142691266592
1103	2	0.19800024550661988
1103	3	0.14486560433517326
1103	4	0.15873146361334278
1103	5	0.16492478202852823
1103	6	0.18041872932975878
1103	7	0.12832489565093397
1103	8	0.14986663560556224
1103	9	0.18009593281269787
1103	10-11	0.19114040252473785
1103	12-13	0.2134789199800835
1103	14-15	0.3396798493567701
1103	16-17	0.4308352411573608
1103	18-19	0.3160755870038159
1103	20-21	0.3286185250093183
1103	22-23	0.3497454583052928
1103	24-25	0.32081590973862717
1103	26-27	0.367587753122109
1103	28-29	0.34984866037362394
1103	30-31	0.3639081047035404
1103	32-33	0.3491664087503281
1103	34-35	0.3546664456383155
1103	36-37	0.35973349986383596
1103	38-39	0.36705625448963275
1103	40-41	0.40075764286807214
1103	42-43	0.4140600272364878
1103	44-45	0.3605599106330146
1103	46-47	0.36100590998107407
1103	48-49	0.4239414607174652
1103	50-51	0.3998468924119223
1103	52-53	0.3586877954210266
1103	54-55	0.35056565220110514
1103	56-57	0.342364941535763
1103	58-59	0.3320068202424338
1103	60-61	0.33562513744758604
1103	62-63	0.317955601384071
1103	64-65	0.3073991775088132
1103	66-67	0.26503390487441436
1103	68-69	0.2872304792706686
1103	70-71	0.2615660818637622
1103	72-73	0.26878786979763447
1103	74-75	0.2587471539848991
1103	76-77	0.271972489839861
1103	78-79	0.24566638315024392
1103	80-81	0.2610293566941593
1103	82-83	0.23445554619501507
1103	84-85	0.2241652743463476
1103	86-87	0.24734603341659067
1103	88-89	0.29850208949748236
1103	90-91	0.20693224617607342
1103	92-93	0.2766643948426122
1103	94-95	0.32548119180902546
1103	96-97	0.2731194530871619
1103	98-99	0.32489130978999725
1103	100	0.34553054553165197
1104	1	0.1846983741368291
1104	2	0.17346796380148533
1104	3	0.17819371804129247
1104	4	0.15837428232690343
1104	5	0.16260051074507942
1104	6	0.17428803222911426
1104	7	0.1501885964882561
1104	8	0.17817510645242862
1104	9	0.21243034098382907
1104	10-11	0.21745775450818883
1104	12-13	0.2501801713223628
1104	14-15	0.36926025382889094
1104	16-17	0.41783528659683355
1104	18-19	0.363871358700365
1104	20-21	0.3278823548677252
1104	22-23	0.36818768505569466
1104	24-25	0.32404922386575663
1104	26-27	0.30480089110962183
1104	28-29	0.2969843941611501
1104	30-31	0.3572606391703985
1104	32-33	0.3666915098362651
1104	34-35	0.34629447608377717
1104	36-37	0.30400028835559567
1104	38-39	0.3306428258921912
1104	40-41	0.33878000888623916
1104	42-43	0.352320122781002
1104	44-45	0.3626746704718897
1104	46-47	0.4255920653337384
1104	48-49	0.5077473138959334
1104	50-51	0.4111399613044142
1104	52-53	0.3579667267251594
1104	54-55	0.4076596323492865
1104	56-57	0.3969937373576329
1104	58-59	0.3887028502682952
1104	60-61	0.37236920155744
1104	62-63	0.39319953449649603
1104	64-65	0.313533531726911
1104	66-67	0.3207830201982773
1104	68-69	0.30043850100999236
1104	70-71	0.3189738393108925
1104	72-73	0.2860301312779825
1104	74-75	0.2668384292175361
1104	76-77	0.2899646208717641
1104	78-79	0.28168901538629854
1104	80-81	0.2941375957152488
1104	82-83	0.2588968053718048
1104	84-85	0.34003851544600394
1104	86-87	0.34290481478059576
1104	88-89	0.3932703507180264
1104	90-91	0.3181692167449839
1104	92-93	0.3008933385032364
1104	94-95	0.30977053618499895
1104	96-97	0.3059833747028833
1104	98-99	0.2664210288101927
1104	100	0.32162566639642876
1105	1	0.1591835134817785
1105	2	0.15528107196973195
1105	3	0.15734525943582156
1105	4	0.11287577981905628
1105	5	0.14121560113586895
1105	6	0.15988684866905345
1105	7	0.15356775668984568
1105	8	0.14152058811566093
1105	9	0.20290773426496145
1105	10-11	0.2105819200622605
1105	12-13	0.2074559384112078
1105	14-15	0.3268065317489999
1105	16-17	0.3795811579688788
1105	18-19	0.2956703940508021
1105	20-21	0.22971085893921384
1105	22-23	0.2368909410486708
1105	24-25	0.25334041091394965
1105	26-27	0.28756613966926636
1105	28-29	0.23715928199958825
1105	30-31	0.30893566533140415
1105	32-33	0.269007991872364
1105	34-35	0.2565773758059322
1105	36-37	0.26221159712764575
1105	38-39	0.25320010947751115
1105	40-41	0.27083427944518235
1105	42-43	0.23599938281540034
1105	44-45	0.27160838389158215
1105	46-47	0.31233126113835397
1105	48-49	0.33233998400262266
1105	50-51	0.27575813087585743
1105	52-53	0.24246473608298658
1105	54-55	0.258152301253169
1105	56-57	0.1793434168101129
1105	58-59	0.18612572040144926
1105	60-61	0.17021906744469817
1105	62-63	0.21050910040418813
1105	64-65	0.21858735724801903
1105	66-67	0.14929647357153897
1105	68-69	0.129015703045134
1105	70-71	0.17106178840818842
1105	72-73	0.17154439053056336
1105	74-75	0.2041127529404605
1105	76-77	0.1132902562534781
1105	78-79	0.1562757917487687
1105	80-81	0.1713500952641951
1105	82-83	0.13569578058199738
1105	84-85	0.14396807918864596
1105	86-87	0.1389724845865885
1105	88-89	0.19900123514050705
1105	90-91	0.16190067851382395
1105	92-93	0.1690884094085945
1105	94-95	0.1890134907550518
1105	96-97	0.1383734684013902
1105	98-99	0.18577512255681228
1105	100	0.1992205376361582
1106	1	0.12808465114517986
1106	2	0.09481608371413586
1106	3	0.11000274877372362
1106	4	0.09934255450419016
1106	5	0.12207508422781643
1106	6	0.11894052675167188
1106	7	0.11787441655620512
1106	8	0.11045856462376946
1106	9	0.12912125538542085
1106	10-11	0.1350407386980237
1106	12-13	0.12403311012393914
1106	14-15	0.23806995840149625
1106	16-17	0.28136644640940034
1106	18-19	0.21162109723094602
1106	20-21	0.15617604534430995
1106	22-23	0.17168966317715473
1106	24-25	0.18456527937005518
1106	26-27	0.17185308481693085
1106	28-29	0.18591169188686507
1106	30-31	0.23299832374981122
1106	32-33	0.16912103731838357
1106	34-35	0.14088299480916078
1106	36-37	0.1672439313480254
1106	38-39	0.22296366777991494
1106	40-41	0.18300781368823493
1106	42-43	0.19300335467200824
1106	44-45	0.23402603137899547
1106	46-47	0.2601381278280712
1106	48-49	0.2966063277704123
1106	50-51	0.30429791941101314
1106	52-53	0.2839839490670997
1106	54-55	0.2329723803510646
1106	56-57	0.19552977140288164
1106	58-59	0.21303058281283427
1106	60-61	0.23395556561874997
1106	62-63	0.1941209802762458
1106	64-65	0.24608639059457715
1106	66-67	0.2544464253256038
1106	68-69	0.1989679813981482
1106	70-71	0.20425924669752504
1106	72-73	0.21821157377374334
1106	74-75	0.19864425080803727
1106	76-77	0.13367865240289944
1106	78-79	0.22541725314373906
1106	80-81	0.2191929912384083
1106	82-83	0.1521955197879592
1106	84-85	0.13943812719557513
1106	86-87	0.21202254123652153
1106	88-89	0.2725458713993163
1106	90-91	0.1575721682318303
1106	92-93	0.23039660474082524
1106	94-95	0.22613551962088962
1106	96-97	0.22942562296879743
1106	98-99	0.1919274640555244
1106	100	0.19898181760482458
1107	1	0.1187445524398072
1107	2	0.0832198792407226
1107	3	0.10546553581354345
1107	4	0.059643320253087495
1107	5	0.0627557403924186
1107	6	0.11028500914252248
1107	7	0.09604646762863212
1107	8	0.07843774868700137
1107	9	0.13229625184688842
1107	10-11	0.17028958884381495
1107	12-13	0.15563426478713183
1107	14-15	0.26589257116156517
1107	16-17	0.32859561940485094
1107	18-19	0.23779119576437324
1107	20-21	0.19657197723920206
1107	22-23	0.1921007059512121
1107	24-25	0.20393200174927983
1107	26-27	0.20737383889553485
1107	28-29	0.22718586987361533
1107	30-31	0.22883199854133807
1107	32-33	0.20327556422113702
1107	34-35	0.18310001998605685
1107	36-37	0.16293594164738323
1107	38-39	0.1566931750829852
1107	40-41	0.1719751170347763
1107	42-43	0.21228456203887447
1107	44-45	0.24905759594583543
1107	46-47	0.2611222265996531
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2216	60-61	-0.7623277435079174
2216	62-63	-0.7374153134341483
2216	64-65	-0.7045723410631766
2216	66-67	-0.6888064941280874
2216	68-69	-0.6443274679665052
2216	70-71	-0.6704564932773351
2216	72-73	-0.5645226696432388
2216	74-75	-0.598729680245313
2216	76-77	-0.5911176794736903
2216	78-79	-0.5831275530618569
2216	80-81	-0.600658922607586
2216	82-83	-0.5821261574406336
2216	84-85	-0.5627540063991461
2216	86-87	-0.4881998387451034
2216	88-89	-0.4312638902898982
2216	90-91	-0.5142601675265084
2216	92-93	-0.4527395951823294
2216	94-95	-0.5035474208698005
2216	96-97	-0.5213438623085622
2216	98-99	-0.542115515252668
2216	100	-0.6788742319982077
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	55.0
8	191.0
9	328.0
10	343.0
11	236.0
12	246.0
13	342.0
14	576.0
15	1187.0
16	2187.0
17	4035.0
18	6757.0
19	10696.0
20	15266.0
21	22834.0
22	33171.0
23	38411.0
24	36745.0
25	32230.0
26	31147.0
27	36203.0
28	45306.0
29	55710.0
30	69357.0
31	87936.0
32	113521.0
33	149137.0
34	218909.0
35	337321.0
36	616569.0
37	1377174.0
38	1643189.0
39	544836.0
40	1153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62070127168115	20.34973581042835	13.597467481440944	24.432095436449558
2	15.372335503534348	20.680652968808964	39.670942006040704	24.276069521615984
3	17.61826628861599	26.526499481231536	30.782406944720297	25.072827285432176
4	12.589096955569234	17.826696069716764	38.83130108496676	30.75290588974724
5	12.989916836309801	38.286744124178774	35.12930348963635	13.59403554987508
6	29.68059009800567	38.71534075166902	17.87488882707846	13.729180323246847
7	25.807379134170265	33.370923887260865	23.22277553830848	17.598921440260387
8	25.82734217303952	34.69262686527782	20.616067363888867	18.86396359779379
9	26.612304942525306	14.660225669830238	20.962083239582853	37.7653861480616
10-11	24.73922209719136	26.012214590550432	28.466220115824182	20.782343196434027
12-13	25.619385589736044	24.084634032529557	28.232262954551945	22.063717423182453
14-15	22.977878770271907	24.900418908809375	26.271417016721198	25.850285304197516
16-17	21.911661613314752	27.669600243327164	27.18618088222723	23.232557261130854
18-19	21.78416135036958	26.901586756546724	29.5351959951566	21.779055897927098
20-21	22.91527904247176	26.03713867440673	29.000421720284418	22.04716056283709
22-23	23.027923993359487	25.82155225104124	28.80549168977823	22.345032065821048
24-25	22.608518307138624	26.094716251007988	29.149662064595738	22.147103377257647
26-27	22.44483952803826	26.306729950323383	28.80779283563343	22.44063768600493
28-29	22.155235947551066	26.415256991028507	28.99745649345979	22.432050567960637
30-31	22.614416670860972	26.040792507828293	28.889321020357578	22.455469800953157
32-33	22.74708338288188	26.424811332996224	28.369828171067724	22.458277113054177
34-35	22.788426896774265	26.27973691265901	28.815377743697223	22.1164584468695
36-37	22.855833125350266	26.09494726748201	28.69044944764456	22.358770159523164
38-39	22.859035307363733	26.04189389085829	28.85647985411868	22.242590947659295
40-41	22.70456587851041	25.734825096721696	28.99219223343871	22.56841679132918
42-43	22.816838613299172	25.927772655262515	28.457426717409085	22.797962014029228
44-45	22.682015138952043	26.41896048561467	28.26179283303417	22.637231542399114
46-47	22.86258883929427	26.15910813535344	27.924960231731266	23.053342793621027
48-49	23.30405002439774	26.132669473912497	27.676766125097142	22.88651437659263
50-51	23.013204041128397	26.342371088821043	27.31002948327817	23.334395386772393
52-53	22.689917821013474	26.7243725611246	27.301967654674762	23.28374196318717
54-55	22.828435294003896	26.652384013078667	27.118933226922774	23.40024746599466
56-57	23.156334612739094	26.406774043255936	27.09537783795487	23.3415135060501
58-59	22.629757854255317	26.479343048946657	27.69480225801849	23.196096838779535
60-61	22.783141394354768	26.70357251743951	27.321254397716793	23.192031690488932
62-63	22.952022524736392	26.37881464650038	27.209594573359507	23.45956825540372
64-65	22.461696136645013	26.515715389036878	27.264917513307868	23.75767096101024
66-67	22.84244648321359	26.319260838201412	27.2264146118355	23.61187806674949
68-69	22.48744503035558	26.11728273181681	27.527686447229367	23.86758579059824
70-71	22.56889770450176	25.99706765405547	27.419865401254718	24.01416924018805
72-73	22.82137413630431	26.112000150367066	27.42083521856947	23.645790494759154
74-75	22.698577104229724	26.42458106616607	27.236297279326994	23.640544550277212
76-77	22.675278288319557	26.32995217569718	27.01888311280609	23.97588642317717
78-79	22.44079859322674	26.385610213202774	27.110702067327765	24.06288912624272
80-81	22.365313760504904	26.350820286598342	27.321497731260603	23.962368221636147
82-83	22.633642779786083	25.900148510803067	27.716227346680782	23.749981362730068
84-85	22.787118025070836	26.212697997318767	27.06014260614225	23.940041371468148
86-87	22.07812110879102	26.53968531651783	27.31299335334066	24.06920022135049
88-89	22.501195487833133	25.9905470617385	27.166688413762042	24.341569036666325
90-91	22.320385375467282	25.860972782089547	27.37455282150541	24.444089020937756
92-93	22.466505756102393	25.948685427678214	27.19831008666345	24.386498729555946
94-95	21.82448908224338	25.87153874200805	27.605765273089624	24.698206902658946
96-97	21.92178502185319	25.921594899813638	27.35948318612731	24.797136892205867
98-99	21.74723944120145	26.2253085861963	27.18886559557588	24.838586377026367
100	22.184463850921027	25.82842600099723	26.90101895154749	25.086091196534248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	97.5
2	195.0
3	324.0
4	221.5
5	179.5
6	429.0
7	1110.5
8	2168.0
9	2707.0
10	2118.0
11	1549.5
12	1637.0
13	1970.0
14	2435.5
15	2973.5
16	3644.5
17	4385.0
18	5237.5
19	6168.5
20	7152.5
21	8346.0
22	9939.0
23	11987.0
24	14316.0
25	17340.0
26	22451.0
27	28357.0
28	34315.5
29	43440.5
30	55041.5
31	65553.0
32	78591.0
33	92120.5
34	103271.5
35	115706.5
36	131166.0
37	150299.0
38	158499.0
39	157601.5
40	158106.5
41	156733.5
42	160365.0
43	171911.5
44	183238.5
45	199987.0
46	213922.0
47	221772.0
48	228716.0
49	223710.0
50	215723.5
51	217046.5
52	217144.0
53	212442.0
54	208981.0
55	193716.0
56	175086.0
57	155685.5
58	136793.5
59	123676.5
60	101478.5
61	78417.5
62	61509.5
63	47638.5
64	34596.5
65	23135.5
66	17006.5
67	13131.0
68	9776.0
69	7136.0
70	5270.5
71	3877.0
72	2819.0
73	2255.0
74	1913.0
75	1407.0
76	933.0
77	554.0
78	259.5
79	137.5
80	75.5
81	54.5
82	36.5
83	9.0
84	5.0
85	2.0
86	0.5
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002855435791911743
2	0.0032168833605081665
3	1.6265140586839042E-4
4	0.05085567290151674
5	0.0022048301684381814
6	0.00468074601332368
7	1.807237842982116E-5
8	2.710856764473174E-4
9	1.807237842982116E-5
10-11	9.939808136401637E-5
12-13	0.0
14-15	2.7108567644731738E-5
16-17	2.710856764473174E-4
18-19	1.0843427057892695E-4
20-21	3.162666225218703E-4
22-23	0.0
24-25	0.0
26-27	3.343390009516915E-4
28-29	0.0
30-31	1.8975997351312218E-4
32-33	2.168685411578539E-4
34-35	1.8975997351312218E-4
36-37	5.4217135289463476E-5
38-39	7.409675156226675E-4
40-41	6.054246773990088E-4
42-43	0.0
44-45	5.421713528946348E-4
46-47	0.0
48-49	1.0843427057892695E-4
50-51	0.0
52-53	3.2530281173678084E-4
54-55	3.7048375781133377E-4
56-57	1.4457902743856928E-4
58-59	8.58437975416505E-4
60-61	6.68678001903383E-4
62-63	2.0783235194294335E-4
64-65	3.524113793815126E-4
66-67	0.0011837407871532859
68-69	0.0025030244125302304
70-71	0.003497005226170394
72-73	0.003243991928152898
74-75	0.002765073899762637
76-77	0.004346407012371989
78-79	0.001373500760666408
80-81	0.001030125570499806
82-83	6.957865695481146E-4
84-85	4.337370823157078E-4
86-87	0.0
88-89	0.0
90-91	1.807237842982116E-5
92-93	8.132570293419521E-5
94-95	6.325332450437405E-5
96-97	3.2530281173678084E-4
98-99	1.0843427057892695E-4
100	0.07219915182713553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5533306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.1862328269136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.30607567855388	28.41443232117708
2	15.863750525480668	14.019187484539236
3	7.081068146570105	9.386571773627645
4	3.9540459166279582	6.9885757392171985
5	2.4040049335556772	5.311196085557005
6	1.5218176607887668	4.034603368783288
7	1.0771725866367579	3.3317339095530376
8	0.7470100021240628	2.6406046302309645
9	0.5467595221969573	2.174331919331411
>10	2.398718835910598	17.29228364773636
>50	0.07093444148042846	2.1096857854686215
>100	0.02625550202837123	2.0877306788595265
>500	0.0011519818152218486	0.33217019034647727
>1k	9.874129844329293E-4	0.7053889954779703
>5k	8.228441536941077E-5	0.23651128372682834
>10k+	1.6456883073882154E-4	0.9349921863672741
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13352	0.24130239679497212	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12364	0.22344688690630882	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6505	0.11756082168598665	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.614475685964232E-5
7	0.0	0.0	0.0	0.0	3.614475685964232E-5
8	0.0	0.0	0.0	0.0	3.614475685964232E-5
9	0.0	0.0	0.0	0.0	3.614475685964232E-5
10-11	0.0	0.0	0.0	0.0	3.614475685964232E-5
12-13	0.0	0.0	0.0	0.0	3.614475685964232E-5
14-15	0.0	0.0	0.0	1.807237842982116E-5	3.614475685964232E-5
16-17	0.0	0.0	0.0	1.807237842982116E-5	3.614475685964232E-5
18-19	0.0	0.0	0.0	3.614475685964232E-5	3.614475685964232E-5
20-21	0.0	0.0	0.0	5.421713528946348E-5	3.614475685964232E-5
22-23	0.0	0.0	0.0	1.355428382236587E-4	3.614475685964232E-5
24-25	0.0	0.0	0.0	3.2530281173678084E-4	3.614475685964232E-5
26-27	0.0	0.0	0.0	8.765103538463262E-4	3.614475685964232E-5
28-29	0.0	0.0	0.0	0.0036235118751791425	3.614475685964232E-5
30-31	9.03618921491058E-6	0.0	0.0	0.01293078676653704	3.614475685964232E-5
32-33	1.807237842982116E-5	0.0	0.0	0.034789328477405734	3.614475685964232E-5
34-35	1.807237842982116E-5	0.0	0.0	0.06984974263125879	3.614475685964232E-5
36-37	1.807237842982116E-5	0.0	0.0	0.12721147176751113	3.614475685964232E-5
38-39	1.807237842982116E-5	0.0	0.0	0.2119347818465127	3.614475685964232E-5
40-41	1.807237842982116E-5	0.0	0.0	0.30512500121988556	3.614475685964232E-5
42-43	1.807237842982116E-5	0.0	0.0	0.40402609217708185	3.614475685964232E-5
44-45	1.807237842982116E-5	0.0	0.0	0.5302706916985975	3.614475685964232E-5
46-47	1.807237842982116E-5	0.0	0.0	0.6832353027286038	3.614475685964232E-5
48-49	1.807237842982116E-5	0.0	0.0	0.8491939538496516	3.614475685964232E-5
50-51	1.807237842982116E-5	0.0	0.0	1.0254177159188376	3.614475685964232E-5
52-53	1.807237842982116E-5	0.0	0.0	1.2029969063702604	3.614475685964232E-5
54-55	1.807237842982116E-5	0.0	0.0	1.3987478733328682	3.614475685964232E-5
56-57	1.807237842982116E-5	0.0	0.0	1.633309272973517	3.614475685964232E-5
58-59	1.807237842982116E-5	0.0	0.0	1.8972382875626255	3.614475685964232E-5
60-61	1.807237842982116E-5	0.0	0.0	2.173456519484012	3.614475685964232E-5
62-63	1.807237842982116E-5	0.0	0.0	2.458042262618406	3.614475685964232E-5
64-65	1.807237842982116E-5	0.0	0.0	2.7582606130945946	3.614475685964232E-5
66-67	1.807237842982116E-5	0.0	0.0	3.102033395586653	3.614475685964232E-5
68-69	1.807237842982116E-5	0.0	0.0	3.485402759218449	3.614475685964232E-5
70-71	1.807237842982116E-5	0.0	0.0	3.895618641007745	3.614475685964232E-5
72-73	1.807237842982116E-5	0.0	0.0	4.317129759315678	3.614475685964232E-5
74-75	1.807237842982116E-5	1.807237842982116E-5	0.0	4.746267421320997	3.614475685964232E-5
76-77	1.807237842982116E-5	1.807237842982116E-5	0.0	5.223604116598649	3.614475685964232E-5
78-79	1.807237842982116E-5	1.807237842982116E-5	0.0	5.740194017825871	3.614475685964232E-5
80-81	1.807237842982116E-5	1.807237842982116E-5	0.0	6.2960280888134506	3.614475685964232E-5
82-83	1.807237842982116E-5	1.807237842982116E-5	0.0	6.8669074148438565	3.614475685964232E-5
84-85	1.807237842982116E-5	1.807237842982116E-5	0.0	7.435609019273469	3.614475685964232E-5
86-87	1.807237842982116E-5	1.807237842982116E-5	0.0	8.039904173020613	3.614475685964232E-5
88	1.807237842982116E-5	1.807237842982116E-5	0.0	8.510120351196916	3.614475685964232E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9720	0.0	56.11799	1
GTATCAA	17830	0.0	43.700012	1
CAACGCA	22475	0.0	33.837696	5
ATCAACG	22480	0.0	33.781487	3
TATCAAC	23525	0.0	32.78118	2
AACGCAG	24795	0.0	31.314701	6
TCAACGC	24960	0.0	30.575705	4
ACGCAGA	26375	0.0	28.86724	7
GTGGTAT	3005	0.0	28.792788	1
CGCAGAG	26845	0.0	28.291805	8
GCAGAGT	29525	0.0	25.421299	9
GTACATG	18725	0.0	23.957249	1
TACATGG	19490	0.0	22.60675	2
GAGTACT	18975	0.0	22.539194	12-13
AGAGTAC	27360	0.0	22.502869	10-11
CAGAGTA	28960	0.0	21.795164	10-11
ACATGGG	19805	0.0	21.415794	3
AGTACTT	20300	0.0	20.547127	12-13
TGGTATC	4020	0.0	20.119297	2
GTACTTT	21160	0.0	20.100815	14-15
>>END_MODULE