FastQCFastQC Report
Mon 16 Jan 2017
ERR1146600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526434
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT551042.1810979427920936No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC139460.552003337510499No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT131610.5209318747293616No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC107090.4238780827047134No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC74670.2955549204926786No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG48580.19228683591180296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9300.023.2658441
GCATCGT10150.019.4483538
CATGGGG45950.019.2365364
GTGATCG5750.018.8000768
GAGTACG9300.018.713831
GTATAAG11950.018.5000931
CTAGTAC13900.018.2655813
TGATCGC5950.018.167069
TAGGCAT12500.017.6769735
CTCGTCG7600.017.3148299
CTAGGCA14550.017.1264514
GGTCGCC5800.017.0439994
GTATTAT8900.016.9123481
GGCGTCG4801.8189894E-1216.6458998
GTATATA14550.016.487361
CTATTGA13000.016.268339
ATGGGGG30950.016.2533635
CGACGAG5950.016.19286224-25
TAGTACT15200.016.0847474
CCCTACG7050.015.9991129
Produced by FastQC (version 0.11.5)