##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146600_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2526434
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30666346320545	31.0	31.0	34.0	28.0	34.0
2	31.34932042554842	31.0	31.0	34.0	28.0	34.0
3	31.504250259456608	33.0	31.0	34.0	28.0	34.0
4	34.96405566106219	37.0	35.0	37.0	32.0	37.0
5	34.99330914640952	37.0	35.0	37.0	32.0	37.0
6	35.16422752385378	37.0	35.0	37.0	32.0	37.0
7	35.06769739482607	37.0	35.0	37.0	32.0	37.0
8	35.072300721095424	37.0	35.0	37.0	32.0	37.0
9	36.68406299155252	39.0	37.0	39.0	32.0	39.0
10-11	36.692196788041954	39.0	37.0	39.0	32.0	39.0
12-13	36.667929381887674	39.0	37.0	39.0	32.0	39.0
14-15	37.94349050875661	40.0	38.0	41.0	32.5	41.0
16-17	37.985958271619204	40.0	38.0	41.0	33.0	41.0
18-19	38.01972780606974	40.0	38.0	41.0	32.0	41.0
20-21	38.108770504196826	40.0	38.0	41.0	33.0	41.0
22-23	38.09301351232607	40.0	38.0	41.0	32.0	41.0
24-25	38.07945309475728	40.0	38.0	41.0	32.0	41.0
26-27	38.08685265476953	40.0	38.0	41.0	32.0	41.0
28-29	38.084454610728	40.0	38.0	41.0	32.5	41.0
30-31	38.07889776657534	40.0	38.0	41.0	32.0	41.0
32-33	38.0095957384994	40.0	38.0	41.0	32.0	41.0
34-35	37.904638118391375	40.0	38.0	41.0	32.0	41.0
36-37	37.80580731576602	40.0	38.0	41.0	31.5	41.0
38-39	37.72799111316583	40.0	38.0	41.0	31.0	41.0
40-41	37.73170979332926	40.0	38.0	41.0	31.0	41.0
42-43	37.551407240402874	40.0	37.0	41.0	31.0	41.0
44-45	37.380993526844556	40.0	37.0	41.0	30.5	41.0
46-47	37.218615249794766	40.0	37.0	41.0	30.0	41.0
48-49	37.078788917501896	40.0	36.0	41.0	30.0	41.0
50-51	36.896815630251965	39.5	36.0	41.0	30.0	41.0
52-53	36.85394492791025	39.0	36.0	41.0	30.0	41.0
54-55	36.7262010406763	39.0	35.0	41.0	30.0	41.0
56-57	36.429013186174664	39.0	35.0	41.0	29.0	41.0
58-59	36.23106263611082	39.0	35.0	40.0	29.0	41.0
60-61	35.9086861164788	38.0	35.0	40.0	28.0	41.0
62-63	35.73696581822442	38.0	34.5	40.0	28.0	41.0
64-65	35.47390452313419	37.0	34.0	40.0	28.0	41.0
66-67	35.14139079033927	37.0	34.0	39.5	28.0	41.0
68-69	34.81368521797917	36.0	34.0	39.0	27.5	41.0
70-71	34.400571121192954	35.5	33.5	39.0	26.5	41.0
72-73	34.031795408073194	35.0	33.0	38.5	26.0	40.0
74-75	33.77170410942855	35.0	33.0	37.0	26.0	39.5
76-77	33.500331296998056	35.0	33.0	37.0	26.0	39.0
78-79	33.14802128217084	35.0	33.0	36.0	26.0	39.0
80-81	32.837822598967556	35.0	32.0	36.0	26.0	38.5
82-83	32.55863818330501	35.0	32.0	36.0	25.0	37.0
84-85	32.399453735977275	35.0	32.0	35.0	25.0	37.0
86-87	32.32435480206489	35.0	32.0	35.0	25.0	36.5
88-89	32.132992193740264	35.0	32.0	35.0	25.0	36.0
90-91	31.929017144322792	35.0	32.0	35.0	25.0	36.0
92-93	31.74091901866425	35.0	32.0	35.0	24.0	36.0
94-95	31.619288491209346	35.0	31.5	35.0	24.0	35.0
96-97	31.54130505685088	35.0	31.0	35.0	24.0	35.0
98-99	31.542805986619875	35.0	32.0	35.0	24.0	35.0
100	30.243676660462928	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24061431537565525
1101	2	0.26351968918553226
1101	3	0.21898780507998694
1101	4	0.2521382297327506
1101	5	0.16482061316737173
1101	6	0.22983198843952124
1101	7	0.19276229069227924
1101	8	0.21859278774234525
1101	9	0.3900824633569826
1101	10-11	0.40760557743243453
1101	12-13	0.3261045974601444
1101	14-15	0.40728870695957653
1101	16-17	0.4683710452958749
1101	18-19	0.3797039346404887
1101	20-21	0.4191267565107921
1101	22-23	0.4056784691413071
1101	24-25	0.4782320676996932
1101	26-27	0.368018686572988
1101	28-29	0.44761343423959943
1101	30-31	0.46708697839185476
1101	32-33	0.4904236294857114
1101	34-35	0.3951587259520011
1101	36-37	0.3723500259639678
1101	38-39	0.3898652439625465
1101	40-41	0.36130400567973453
1101	42-43	0.39729813998589236
1101	44-45	0.34929095676717026
1101	46-47	0.2856185073440045
1101	48-49	0.28170465153134217
1101	50-51	0.3426993225710433
1101	52-53	0.3155904879334699
1101	54-55	0.3210479197083487
1101	56-57	0.2828349348078518
1101	58-59	0.3070112085774568
1101	60-61	0.39639676281902325
1101	62-63	0.3257162790319228
1101	64-65	0.3400440738803212
1101	66-67	0.36041608230311795
1101	68-69	0.2961603742128034
1101	70-71	0.29269155156139703
1101	72-73	0.2527481598121568
1101	74-75	0.3470096882944347
1101	76-77	0.33148510021769084
1101	78-79	0.3773901007586389
1101	80-81	0.25141029823566186
1101	82-83	0.2774021128775743
1101	84-85	0.2873840814236459
1101	86-87	0.3436723312655303
1101	88-89	0.34024239359870734
1101	90-91	0.3517487600129101
1101	92-93	0.3371518051090163
1101	94-95	0.32247789995182075
1101	96-97	0.23052878325298565
1101	98-99	0.3268466689497629
1101	100	0.3583779601234518
1102	1	0.209738944694017
1102	2	0.22047184309541734
1102	3	0.26396255988409933
1102	4	0.2552788310452172
1102	5	0.15706823987206064
1102	6	0.2769754437302723
1102	7	0.3083127688784373
1102	8	0.3041915305365066
1102	9	0.2627892586711056
1102	10-11	0.35974885961698533
1102	12-13	0.33684776326906274
1102	14-15	0.44172746648703054
1102	16-17	0.3828927807055038
1102	18-19	0.4342027327822322
1102	20-21	0.35034344806979334
1102	22-23	0.36235253930807687
1102	24-25	0.39516336718520506
1102	26-27	0.47406790832371115
1102	28-29	0.41702510427078465
1102	30-31	0.4032387282078176
1102	32-33	0.4400897758437097
1102	34-35	0.40126547650912414
1102	36-37	0.45566469507633656
1102	38-39	0.4201074543458887
1102	40-41	0.3981759051411373
1102	42-43	0.41968290671437103
1102	44-45	0.3346977790821484
1102	46-47	0.46377843575506716
1102	48-49	0.4654943834654759
1102	50-51	0.5269867000287931
1102	52-53	0.40471711655884945
1102	54-55	0.40608525078013
1102	56-57	0.40195492785019127
1102	58-59	0.36924650807856807
1102	60-61	0.45517903447917973
1102	62-63	0.4544428773242757
1102	64-65	0.436961641820794
1102	66-67	0.4659818597360754
1102	68-69	0.4498493509119612
1102	70-71	0.42557395174711843
1102	72-73	0.4441159057921311
1102	74-75	0.4125827352596758
1102	76-77	0.3648574234412223
1102	78-79	0.29047816410677996
1102	80-81	0.3439100845214398
1102	82-83	0.3117636939563013
1102	84-85	0.37640044186250066
1102	86-87	0.33775493011962254
1102	88-89	0.3291221996974798
1102	90-91	0.3357695260742162
1102	92-93	0.4298537497367505
1102	94-95	0.38022343717647544
1102	96-97	0.40136775800760205
1102	98-99	0.5007207569747827
1102	100	0.4606440920203525
1103	1	0.18532977010734086
1103	2	0.16893865226691318
1103	3	0.20567741716247667
1103	4	0.29243049687543277
1103	5	0.2742903120060447
1103	6	0.29448521131469363
1103	7	0.285446255600192
1103	8	0.3121473480706385
1103	9	0.4211082985136514
1103	10-11	0.4148632347522607
1103	12-13	0.4342363771717288
1103	14-15	0.5155161534853008
1103	16-17	0.47972501488387564
1103	18-19	0.4604898535088182
1103	20-21	0.4067482748372555
1103	22-23	0.37732787831878056
1103	24-25	0.4110958478418212
1103	26-27	0.3836921453093467
1103	28-29	0.38405243033562897
1103	30-31	0.47932100043487935
1103	32-33	0.4375991010627942
1103	34-35	0.48926787054814724
1103	36-37	0.42851763897370176
1103	38-39	0.386590688612209
1103	40-41	0.4189896326500602
1103	42-43	0.41192259885619364
1103	44-45	0.3544117811208878
1103	46-47	0.38206390498444165
1103	48-49	0.4492624723893357
1103	50-51	0.45901015803015355
1103	52-53	0.44049749375955116
1103	54-55	0.2925011068386638
1103	56-57	0.392462022459668
1103	58-59	0.4040938367449556
1103	60-61	0.4158660102784637
1103	62-63	0.4716965733129115
1103	64-65	0.4516645399631898
1103	66-67	0.4375306158556782
1103	68-69	0.48550601503151825
1103	70-71	0.47478538288847716
1103	72-73	0.43343010612288424
1103	74-75	0.390634349463042
1103	76-77	0.3035287809357712
1103	78-79	0.2929665129588912
1103	80-81	0.3037227305173573
1103	82-83	0.2854070586782598
1103	84-85	0.3800989758602924
1103	86-87	0.2873558270679766
1103	88-89	0.25057659332433246
1103	90-91	0.3901126144407385
1103	92-93	0.41943156996052977
1103	94-95	0.38216229631980525
1103	96-97	0.34708755748971853
1103	98-99	0.29916976620809876
1103	100	0.3316746750876973
1104	1	0.18628378085233166
1104	2	0.09022840028933743
1104	3	0.09270368592691725
1104	4	0.1314419112443801
1104	5	0.04608558677161767
1104	6	0.13093552301252487
1104	7	0.08724426608907265
1104	8	0.2371541873789056
1104	9	0.21218401984239676
1104	10-11	0.25365258987825
1104	12-13	0.25363294645828915
1104	14-15	0.2882296417653478
1104	16-17	0.330282636783636
1104	18-19	0.33653655870843835
1104	20-21	0.3930906127747491
1104	22-23	0.3837262694806469
1104	24-25	0.3885002284915231
1104	26-27	0.3303874497604298
1104	28-29	0.4234931204363903
1104	30-31	0.41466479936503475
1104	32-33	0.4790103850483405
1104	34-35	0.3141818698845853
1104	36-37	0.3689181605073344
1104	38-39	0.3952323728864613
1104	40-41	0.3945642800618927
1104	42-43	0.4123518745879977
1104	44-45	0.37440031436575083
1104	46-47	0.32938027683326254
1104	48-49	0.35811748487471107
1104	50-51	0.3837364401821759
1104	52-53	0.43855833158936264
1104	54-55	0.2770768660018845
1104	56-57	0.3920601039609437
1104	58-59	0.4047748572279559
1104	60-61	0.38500646929790605
1104	62-63	0.3732742707652008
1104	64-65	0.42687161093483894
1104	66-67	0.4005357788058319
1104	68-69	0.28334479361836884
1104	70-71	0.3449575588362208
1104	72-73	0.43131574124724636
1104	74-75	0.4536127372489176
1104	76-77	0.3469793429231913
1104	78-79	0.3248170260525214
1104	80-81	0.17336522386722208
1104	82-83	0.29181097949362567
1104	84-85	0.28068017154600255
1104	86-87	0.26612545006202737
1104	88-89	0.22898096815335123
1104	90-91	0.2963512277200522
1104	92-93	0.306527104910856
1104	94-95	0.23830224893940866
1104	96-97	0.21096989810473232
1104	98-99	0.23313609934935187
1104	100	0.3348975899717779
1105	1	0.06175742123773986
1105	2	0.04332345838603757
1105	3	0.07894242982888144
1105	4	0.009839718179691204
1105	5	-0.08625798212158742
1105	6	0.08296664924262132
1105	7	0.18269734247817837
1105	8	0.12990519801380884
1105	9	0.19550975844196472
1105	10-11	0.2565930165189414
1105	12-13	0.2434132184313924
1105	14-15	0.3192710784989359
1105	16-17	0.2662369896902277
1105	18-19	0.23813863935767898
1105	20-21	0.37635432213907904
1105	22-23	0.29144182308053246
1105	24-25	0.3248368340510268
1105	26-27	0.28046547812203926
1105	28-29	0.24205572038074763
1105	30-31	0.25900996134564735
1105	32-33	0.2958390570887346
1105	34-35	0.20174824016258697
1105	36-37	0.32599312675259284
1105	38-39	0.3071211891960175
1105	40-41	0.291318244011066
1105	42-43	0.3150567105798885
1105	44-45	0.2998173656203704
1105	46-47	0.2609241302981289
1105	48-49	0.27239701631108915
1105	50-51	0.3180573565607432
1105	52-53	0.2678811369488514
1105	54-55	0.25545030287519666
1105	56-57	0.2767009024960103
1105	58-59	0.20819598033048692
1105	60-61	0.2982037612098978
1105	62-63	0.2971813818850535
1105	64-65	0.2893566907959695
1105	66-67	0.27442311685231857
1105	68-69	0.19344475905062097
1105	70-71	0.17878657735707293
1105	72-73	0.17419191443286763
1105	74-75	0.23259675056853268
1105	76-77	0.22657011859375586
1105	78-79	0.2208412933346935
1105	80-81	0.10345879767859856
1105	82-83	0.05697303237625562
1105	84-85	0.08246324105768821
1105	86-87	0.12199676810498516
1105	88-89	0.20753888679749366
1105	90-91	0.09362374804616991
1105	92-93	0.14706844950393005
1105	94-95	0.03079570776584717
1105	96-97	0.12596431145872344
1105	98-99	0.13048148897123824
1105	100	0.09688792144075364
1106	1	0.06061560754396922
1106	2	0.15936194710317508
1106	3	0.10599259702607
1106	4	0.11316840626614066
1106	5	0.07317878291848956
1106	6	0.0973213976975913
1106	7	0.09790799113768855
1106	8	0.1899825215412747
1106	9	0.24744020608288508
1106	10-11	0.17440895200705597
1106	12-13	0.2188231273824428
1106	14-15	0.21997345591015716
1106	16-17	0.22649762817030705
1106	18-19	0.24272012137726762
1106	20-21	0.24337721632711862
1106	22-23	0.30123426536721354
1106	24-25	0.34221208837269046
1106	26-27	0.273250757377717
1106	28-29	0.23620851619279648
1106	30-31	0.240020997280034
1106	32-33	0.2867492396436049
1106	34-35	0.18656224175062164
1106	36-37	0.19023355079252724
1106	38-39	0.19525360277922488
1106	40-41	0.1484021707583807
1106	42-43	0.20407159993251867
1106	44-45	0.21546948413053002
1106	46-47	0.2063723901785437
1106	48-49	0.2367399461012596
1106	50-51	0.22503054663294364
1106	52-53	0.2126939647892172
1106	54-55	0.15812408151457902
1106	56-57	0.21158755968351528
1106	58-59	0.16729288433517553
1106	60-61	0.23054374163042723
1106	62-63	0.2429790211367191
1106	64-65	0.28774622857522303
1106	66-67	0.13816647653723635
1106	68-69	0.1419124809509853
1106	70-71	0.15874966848264904
1106	72-73	0.17491932096934448
1106	74-75	0.1619789332489887
1106	76-77	0.12935995752665974
1106	78-79	0.141708083567039
1106	80-81	0.1797242124920828
1106	82-83	0.14978419129898413
1106	84-85	0.14191874121375747
1106	86-87	0.09065298862356741
1106	88-89	0.04682128314176737
1106	90-91	0.0895141234157748
1106	92-93	0.12049937967267255
1106	94-95	0.12984392830484026
1106	96-97	0.03405526808859349
1106	98-99	0.0830112051616787
1106	100	0.1031055365787914
1107	1	0.046085627122476325
1107	2	0.07470392757453581
1107	3	0.058432287042833764
1107	4	0.07555046127425413
1107	5	0.008797908954463196
1107	6	0.07172303379186218
1107	7	0.03312631878671368
1107	8	0.038416606399046316
1107	9	0.08581399349580465
1107	10-11	0.12257276663328298
1107	12-13	0.158608292568843
1107	14-15	0.1223334447961335
1107	16-17	0.12810749930294918
1107	18-19	0.1035586798729824
1107	20-21	0.17807967905193323
1107	22-23	0.23577856533922414
1107	24-25	0.32280890396717155
1107	26-27	0.2005468685729781
1107	28-29	0.23774565037112438
1107	30-31	0.2231936983960665
1107	32-33	0.15936417040808948
1107	34-35	0.2662943960842199
1107	36-37	0.21209382849120573
1107	38-39	0.21391315356692076
1107	40-41	0.1862847472916016
1107	42-43	0.24622922790535995
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2216	70-71	-1.090289115395798
2216	72-73	-1.0043959812547314
2216	74-75	-0.8897675630663642
2216	76-77	-0.9912029375901028
2216	78-79	-0.8872506066344883
2216	80-81	-0.9418989093359329
2216	82-83	-0.9556435467897906
2216	84-85	-0.8463207360641789
2216	86-87	-0.8489808999469446
2216	88-89	-0.9542899354255923
2216	90-91	-0.9701837927506958
2216	92-93	-0.934018515607896
2216	94-95	-1.0775756279751363
2216	96-97	-0.9599688958805714
2216	98-99	-0.9675598419204654
2216	100	-1.0342822869474269
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	754.0
1	211.0
2	145.0
3	110.0
4	103.0
5	158.0
6	218.0
7	527.0
8	1425.0
9	2206.0
10	2634.0
11	3168.0
12	4008.0
13	4840.0
14	6081.0
15	6764.0
16	7389.0
17	7769.0
18	8472.0
19	9489.0
20	10795.0
21	11942.0
22	13467.0
23	15705.0
24	19093.0
25	22854.0
26	24948.0
27	24679.0
28	27959.0
29	34063.0
30	40217.0
31	48441.0
32	61795.0
33	83998.0
34	124769.0
35	193573.0
36	329120.0
37	580792.0
38	616353.0
39	175103.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.710578051492064	19.40583891516592	13.300913405193898	22.582669628148125
2	14.017792462463023	22.936679160179295	37.88889078124523	25.156637596112446
3	22.016384285980266	25.657570007566243	30.117930414321197	22.208115292132295
4	11.659202595555627	17.22150365544712	41.1273157272369	29.991978021760357
5	12.093377210861313	40.08439570748156	34.97382837770011	12.84839870395702
6	28.40406713587031	37.98441842452959	18.301160595609982	15.310353843990118
7	25.2402841455844	33.24143619421448	23.37688114130463	18.14139851889649
8	24.587145277718463	32.032336671561964	21.125801394742798	22.25471665597678
9	24.143002687252032	18.595158192949764	22.842672167927898	34.4191669518703
10-11	24.85252832078175	25.383153031849766	28.09910382061676	21.665214826751725
12-13	27.460888281714485	23.82831206352463	26.92865124234194	21.782148412418945
14-15	23.38439092950257	25.575244691420234	27.592790218998225	23.447574160078975
16-17	21.382358976471714	28.400735075658602	27.95010707639067	22.26679887147902
18-19	21.01285123110013	30.746099500928644	27.064792126885383	21.176257141085852
20-21	23.30584313176383	27.308938279839968	25.605260088097246	23.77995850029896
22-23	22.021379291495066	27.561983585654026	26.030531206389863	24.386105916461048
24-25	24.20793875518633	27.641333118895144	25.34748493320858	22.803243192709946
26-27	23.43014237548087	25.69205241876707	28.54736527724084	22.33043992851122
28-29	21.527392779280138	29.912645202616662	26.529610172556573	22.030351845546626
30-31	22.80776363872977	27.04689642030944	26.13005146668056	24.015288474280226
32-33	22.81776950416633	27.77531387375167	26.731943506587275	22.674973115494726
34-35	24.202969516461174	27.157570254442824	26.634172971679536	22.005287257416462
36-37	23.458371117626715	26.027604026151685	28.41448191246563	22.099542943755974
38-39	22.843565564556307	27.7955052596445	27.45794909024641	21.90298008555279
40-41	22.507397220332777	28.346774241470467	26.819375507822297	22.326453030374456
42-43	22.221555322867108	28.042259497274095	25.18508437993613	24.551100799922665
44-45	23.13342383063469	26.466419763406275	26.10798944061694	24.292166965342098
46-47	24.296962679114227	27.951841641455026	26.61132980786334	21.139865871567412
48-49	23.48771557688266	27.19232683873133	27.05698988994166	22.262967694444352
50-51	20.537652875326867	27.87180588842253	29.08141789148177	22.509123344768835
52-53	22.283108351579692	29.03164971585996	25.38536251403763	23.299879418522718
54-55	21.55499744770974	26.98989998231125	26.696183568400883	24.75891900157812
56-57	23.744689870615584	25.67811676985659	26.549983310668196	24.02721004885963
58-59	25.20210899790903	26.188876749615435	26.155615360462132	22.453398892013404
60-61	23.128150421905193	27.10464774209652	28.582539196721036	21.18466263927725
62-63	21.56359525916957	27.54333888284456	27.81285732737547	23.080208530610403
64-65	20.85326036676241	29.826373556274415	26.368346074550235	22.952020002412937
66-67	22.911651200938245	26.753940392890257	25.461574730769538	24.872833675401964
68-69	22.08774772775898	26.94681727774828	26.48852587620942	24.47690911828332
70-71	23.918043549665853	26.943547565907533	25.779029643679145	23.35937924074747
72-73	23.36043433446773	25.455956300304265	28.70252958305294	22.48107978217507
74-75	21.870208198452993	29.459510539883244	26.28080521680834	22.38947604485542
76-77	22.36700356310677	26.613788624921302	26.39749817842951	24.621709633542412
78-79	22.89706202255935	27.035181474500376	27.01626116446881	23.05149533847147
80-81	23.960071730940825	26.403080024533693	26.81017213206988	22.826676112455605
82-83	22.949220123660456	25.727231789165835	28.57188990023043	22.75165818694328
84-85	22.424866409692	27.618668191847036	27.154873626827538	22.80159177163343
86-87	22.14857224814829	28.099322867068903	26.74489852316834	23.00720636161446
88-89	22.00670290307257	27.568095797868526	25.441158233758493	24.984043065300412
90-91	23.111208224509976	25.960531195324187	26.10044976798438	24.827810812181454
92-93	24.224001369190866	27.220453397889205	26.467022051460738	22.08852318145919
94-95	22.887829556945917	26.773548483584925	27.126502127963033	23.212119831506133
96-97	21.070503019113026	27.098131523311537	28.86011769816476	22.971247759410673
98-99	22.615063184258396	28.17489358346848	25.804666676440252	23.40537655583287
100	22.46216574500579	25.09997940696013	25.81442695204361	26.62342789599047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2027.0
1	1332.5
2	621.0
3	600.0
4	575.5
5	482.0
6	383.0
7	380.0
8	432.0
9	444.0
10	376.0
11	322.5
12	329.5
13	336.0
14	379.5
15	465.5
16	545.5
17	636.5
18	777.0
19	982.0
20	1262.0
21	1690.5
22	2466.5
23	3582.0
24	4931.0
25	6724.0
26	9469.5
27	12726.0
28	15751.0
29	19623.5
30	23991.0
31	28151.0
32	33111.0
33	38547.5
34	43789.5
35	48328.0
36	52626.0
37	58251.5
38	61885.5
39	62660.0
40	63141.0
41	63684.5
42	65364.0
43	70169.0
44	77396.0
45	85121.5
46	96042.0
47	137423.5
48	218128.5
49	220168.5
50	155005.0
51	116144.0
52	89026.5
53	77008.0
54	70911.5
55	63093.0
56	55851.5
57	48526.0
58	41765.5
59	37127.5
60	30976.5
61	24162.0
62	19020.5
63	14877.5
64	10876.5
65	7617.0
66	5597.0
67	4161.0
68	3103.5
69	2531.5
70	2229.0
71	1875.5
72	1526.0
73	1417.5
74	1176.0
75	853.5
76	633.5
77	455.0
78	331.5
79	228.0
80	164.0
81	126.5
82	93.5
83	65.0
84	50.0
85	48.5
86	34.0
87	22.5
88	19.5
89	14.5
90	11.5
91	8.0
92	6.5
93	4.5
94	7.0
95	8.0
96	4.0
97	2.5
98	4.5
99	5.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09784542164964531
2	0.06558651443101225
3	0.08169617729970385
4	0.08387315876844596
5	0.08450646246844366
6	0.09099782539342013
7	0.08846461059342932
8	0.08870209948092846
9	0.07607560696222422
10-11	0.08118161804345572
12-13	0.07152373661849072
14-15	0.08035040693720873
16-17	0.08185450322470328
18-19	0.08096391989658151
20-21	0.07273097179661135
22-23	0.07659016621847237
24-25	0.07947961434971189
26-27	0.0729090884622357
28-29	0.08207220137157749
30-31	0.08626783838406228
32-33	0.08749486430280784
34-35	0.08913749577467688
36-37	0.08854377355592903
38-39	0.08951351984655051
40-41	0.08608972171843793
42-43	0.09163112909341783
44-45	0.08840523837155453
46-47	0.08458562543094338
48-49	0.09355083093403588
50-51	0.09014682354654822
52-53	0.09556948647777856
54-55	0.08828649392780495
56-57	0.09366957537778545
58-59	0.09841935312776824
60-61	0.09762772350277109
62-63	0.09847872534964303
64-65	0.10047759015276077
66-67	0.10188273273713068
68-69	0.09871621423714215
70-71	0.10120984755588311
72-73	0.09818186424026909
74-75	0.10259519939962809
76-77	0.09946826238088943
78-79	0.10651376604336388
80-81	0.10124942903713297
82-83	0.10562318271524211
84-85	0.10210043088400489
86-87	0.09830060868401866
88-89	0.09709337350589804
90-91	0.09760793276214617
92-93	0.09165091983404276
94-95	0.09129468650279406
96-97	0.08967184577154994
98-99	0.10390138828087335
100	0.24401983190536544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2526434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.414329037646695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51118225477875	45.76054263979044
2	14.588763113430847	17.9191819620105
3	5.243919580068298	9.661554076118177
4	2.400218269938793	5.896311783687684
5	1.2169437770084914	3.736889277075802
6	0.6641516678694872	2.447305743686312
7	0.4303588708935014	1.8501140910926528
8	0.2596170109412892	1.2755363626974927
9	0.1776077680745857	0.9816895717357184
>10	0.5015237408355794	4.332002505211356
>50	0.004181530506503032	0.172875946911013
>100	5.960798641297861E-4	0.07304616790902642
>500	1.7024287087604722E-4	0.08364281350636131
>1k	3.4048574175209444E-4	0.5614001089030088
>5k	8.512143543802361E-5	0.39035055528249996
>10k+	3.4048574175209444E-4	4.857556394381934
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	55104	2.1810979427920936	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	13946	0.552003337510499	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13161	0.5209318747293616	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	10709	0.4238780827047134	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7467	0.2955549204926786	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	4858	0.19228683591180296	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.916296249971304E-5
2	0.0	0.0	0.0	0.0	7.916296249971304E-5
3	0.0	0.0	0.0	0.0	7.916296249971304E-5
4	0.0	0.0	0.0	0.0	7.916296249971304E-5
5	0.0	0.0	0.0	0.0	7.916296249971304E-5
6	0.0	0.0	0.0	0.0	1.1874444374956955E-4
7	0.0	0.0	0.0	0.0	1.1874444374956955E-4
8	0.0	0.0	0.0	0.0	1.1874444374956955E-4
9	0.0	0.0	0.0	3.958148124985652E-5	1.1874444374956955E-4
10-11	0.0	0.0	0.0	3.958148124985652E-5	1.5832592499942608E-4
12-13	0.0	0.0	0.0	3.958148124985652E-5	3.1665184999885216E-4
14-15	0.0	0.0	0.0	9.895370312464129E-5	3.364425906237804E-4
16-17	0.0	0.0	0.0	1.385351843744978E-4	3.9581481249856515E-4
18-19	0.0	0.0	0.0	2.1769814687421082E-4	3.9581481249856515E-4
20-21	0.0	0.0	0.0	5.145592562481347E-4	4.353962937484217E-4
22-23	0.0	0.0	0.0	8.905833281217716E-4	4.353962937484217E-4
24-25	0.0	0.0	0.0	0.0017613759156186151	4.353962937484217E-4
26-27	0.0	0.0	0.0	0.0028498666499896692	4.5518703437334994E-4
28-29	0.0	0.0	0.0	0.006748642553100537	4.749777749982782E-4
30-31	0.0	0.0	0.0	0.01866266840930735	4.749777749982782E-4
32-33	0.0	0.0	0.0	0.04597389047170834	4.749777749982782E-4
34-35	0.0	0.0	0.0	0.08678239764031041	4.749777749982782E-4
36-37	0.0	3.958148124985652E-5	0.0	0.1470056213619671	4.749777749982782E-4
38-39	0.0	3.958148124985652E-5	0.0	0.23620248935851876	5.34349996873063E-4
40-41	0.0	3.958148124985652E-5	0.0	0.3356509609987832	5.541407374979912E-4
42-43	0.0	3.958148124985652E-5	0.0	0.4359108530046698	5.541407374979912E-4
44-45	0.0	3.958148124985652E-5	0.0	0.5626507559667104	5.937222187478478E-4
46-47	0.0	3.958148124985652E-5	0.0	0.7171768587661502	6.530944406226326E-4
48-49	0.0	3.958148124985652E-5	0.0	0.8713665189749662	6.728851812475608E-4
50-51	0.0	3.958148124985652E-5	0.0	1.0431105661180937	6.728851812475608E-4
52-53	0.0	3.958148124985652E-5	0.0	1.2170513854705882	6.728851812475608E-4
54-55	0.0	3.958148124985652E-5	0.0	1.4127422287698788	6.728851812475608E-4
56-57	0.0	3.958148124985652E-5	0.0	1.6643814958158416	6.728851812475608E-4
58-59	0.0	3.958148124985652E-5	0.0	1.916416577674303	6.728851812475608E-4
60-61	0.0	3.958148124985652E-5	0.0	2.1868174668326974	6.728851812475608E-4
62-63	0.0	3.958148124985652E-5	0.0	2.464778418909815	7.124666624974173E-4
64-65	0.0	3.958148124985652E-5	0.0	2.749230733911909	7.124666624974173E-4
66-67	0.0	3.958148124985652E-5	0.0	3.079221543091963	7.322574031223456E-4
68-69	0.0	3.958148124985652E-5	0.0	3.455225824224975	7.71838884372202E-4
70-71	0.0	3.958148124985652E-5	0.0	3.870831377348468	8.114203656220587E-4
72-73	0.0	3.958148124985652E-5	0.0	4.264112975047043	8.707925874968434E-4
74-75	0.0	3.958148124985652E-5	0.0	4.6682003171268285	9.301648093716281E-4
76-77	0.0	3.958148124985652E-5	0.0	5.0894264405878005	9.499555499965564E-4
78-79	0.0	3.958148124985652E-5	0.0	5.632563526298332	9.499555499965564E-4
80-81	0.0	3.958148124985652E-5	0.0	6.193452906349424	9.89537031246413E-4
82-83	0.0	3.958148124985652E-5	0.0	6.727228971744363	0.0010291185124962695
84-85	0.0	3.958148124985652E-5	0.0	7.26480485933929	0.0010291185124962695
86-87	0.0	3.958148124985652E-5	0.0	7.807961735790446	0.001088490734371054
88	0.0	3.958148124985652E-5	0.0	8.260140577588807	0.001147862956245839
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	930	0.0	23.265844	1
GCATCGT	1015	0.0	19.448353	8
CATGGGG	4595	0.0	19.236536	4
GTGATCG	575	0.0	18.800076	8
GAGTACG	930	0.0	18.71383	1
GTATAAG	1195	0.0	18.500093	1
CTAGTAC	1390	0.0	18.265581	3
TGATCGC	595	0.0	18.16706	9
TAGGCAT	1250	0.0	17.676973	5
CTCGTCG	760	0.0	17.314829	9
CTAGGCA	1455	0.0	17.126451	4
GGTCGCC	580	0.0	17.04399	94
GTATTAT	890	0.0	16.912348	1
GGCGTCG	480	1.8189894E-12	16.645899	8
GTATATA	1455	0.0	16.48736	1
CTATTGA	1300	0.0	16.26833	9
ATGGGGG	3095	0.0	16.253363	5
CGACGAG	595	0.0	16.192862	24-25
TAGTACT	1520	0.0	16.084747	4
CCCTACG	705	0.0	15.999112	9
>>END_MODULE