FastQCFastQC Report
Mon 16 Jan 2017
ERR1146600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526434
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT580982.299604897654164No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC154730.6124442593790299No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT137090.542622526454283No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC122150.4834877934669974No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA108100.42787581231094896No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA95100.37641988668613546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70180.27778283541149307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68680.27184561322401457No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG52520.2078819395242464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.1496179991244576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10350.023.6296771
GAGTACT79650.023.27712212-13
GACGTTA3650.023.1769527
CGTTAAC2957.2759576E-1222.3039979
TAGTACT14400.021.2285254
GTACTTT87950.020.78652214-15
CTAGTAC14350.020.6470323
TAGGCAT13350.020.4200955
CTAACGC3252.5465852E-1120.2587973
CTAGGCA15300.019.979794
CTGTGCG13700.019.896849
GAGTACG8750.019.8878781
CCGTCGT2601.6862032E-819.8836429
CGACGAG5350.019.76524-25
TAACGCC4550.019.638634
AGTACTT85300.019.53148512-13
CATGGGG43600.019.5236534
TACCCCG2001.4081674E-518.8005715
ACTTTTT99450.018.47736416-17
CCTAGTA14450.018.226632
Produced by FastQC (version 0.11.5)