FastQCFastQC Report
Mon 16 Jan 2017
ERR1146599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4343774
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2000814.6061558451245395No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1613553.714626958032347No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG389330.8962943283881712No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG381990.8793965800246514No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT283820.6533949510264576No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG272500.6273346633595579No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA235070.5411653552878212No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107080.24651374588088606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100930.23235555072616576No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG93230.21462902996334526No Hit
GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44310.10200806948059453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTC100850.022.2357754
ATACTCT103100.021.9324025
GCTTATA106800.020.9592481
GTACTAG11150.020.2444651
TTATACT111500.020.0280463
GTACCAG30300.019.7106551
GTACCCG5100.019.3637331
GCATCGT9050.019.2131168
TCGCCGG2753.1634045E-818.82372394
CTCGTCG6900.018.3884149
GCTATTG15200.017.932018
CGACGAG5050.017.68059324-25
CATGGGG44900.017.380874
TGATCGC5850.016.8691439
GGCGTCG6150.016.8109478
CTGTGCG14550.016.4716389
CCTACAC16650.016.376973
CTAACGC4601.2732926E-1116.3524173
TACCTGG33500.016.2791982
CTAGTAC17100.016.2209283
Produced by FastQC (version 0.11.5)