##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146599_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4343774
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51956524441649	31.0	31.0	34.0	30.0	34.0
2	31.687326964984827	33.0	31.0	34.0	30.0	34.0
3	31.870002214664023	33.0	31.0	34.0	30.0	34.0
4	35.32785775687225	37.0	35.0	37.0	33.0	37.0
5	35.45367070202087	37.0	35.0	37.0	33.0	37.0
6	35.50266335219097	37.0	35.0	37.0	33.0	37.0
7	35.48232021279192	37.0	35.0	37.0	33.0	37.0
8	35.499353787743104	37.0	35.0	37.0	33.0	37.0
9	37.21248550223837	39.0	38.0	39.0	34.0	39.0
10-11	37.21094444600479	39.0	38.0	39.0	34.0	39.0
12-13	37.19439938634008	39.0	38.0	39.0	34.0	39.0
14-15	38.42194092049909	40.0	38.0	41.0	34.0	41.0
16-17	38.43880125899736	40.0	38.0	41.0	34.0	41.0
18-19	38.59955444274955	40.0	38.5	41.0	34.0	41.0
20-21	38.66017223732174	40.0	39.0	41.0	34.0	41.0
22-23	38.66362453479394	40.5	39.0	41.0	34.0	41.0
24-25	38.63399223808605	40.5	39.0	41.0	34.0	41.0
26-27	38.49102669706113	40.0	38.5	41.0	34.0	41.0
28-29	38.39216393394315	40.0	38.0	41.0	34.0	41.0
30-31	38.41218258592643	40.0	38.0	41.0	34.0	41.0
32-33	38.423067475425746	40.0	38.0	41.0	34.0	41.0
34-35	38.37206505218734	40.0	38.0	41.0	34.0	41.0
36-37	38.14566192900459	40.0	38.0	41.0	33.0	41.0
38-39	37.96942543511702	40.0	38.0	41.0	32.5	41.0
40-41	37.87463125383595	40.0	38.0	41.0	33.0	41.0
42-43	37.86094741577255	40.0	38.0	41.0	32.5	41.0
44-45	37.89088785466279	40.0	38.0	41.0	33.0	41.0
46-47	37.76749020552174	40.0	38.0	41.0	32.0	41.0
48-49	37.588257123874314	40.0	37.0	41.0	32.0	41.0
50-51	37.395938646900134	40.0	37.0	41.0	31.0	41.0
52-53	37.0662716338373	39.5	36.0	41.0	30.5	41.0
54-55	37.00909554686777	39.0	36.0	41.0	30.5	41.0
56-57	36.938128341852035	39.0	36.0	41.0	30.0	41.0
58-59	36.803569430637964	39.0	35.5	41.0	30.0	41.0
60-61	36.44996747068333	39.0	35.0	40.5	29.5	41.0
62-63	36.04803691444352	38.5	35.0	40.0	28.5	41.0
64-65	35.45059227298658	37.5	34.0	40.0	28.0	41.0
66-67	35.22062404719951	37.0	34.0	39.5	28.0	41.0
68-69	35.14206333018247	37.0	34.0	39.0	28.0	41.0
70-71	34.753370110875935	36.0	34.0	39.0	28.0	41.0
72-73	34.154339291132544	35.5	33.0	38.0	26.5	40.0
74-75	33.658030436205934	35.0	33.0	37.0	26.0	39.5
76-77	33.20344417089839	35.0	32.5	37.0	26.0	39.0
78-79	32.93443362845305	35.0	32.0	36.0	25.5	39.0
80-81	32.81535687630158	35.0	32.0	36.0	26.0	38.0
82-83	32.6129611024883	35.0	32.0	35.5	26.0	37.0
84-85	32.43751217259461	35.0	32.0	35.0	25.0	37.0
86-87	32.020999250881836	35.0	31.5	35.0	25.0	36.5
88-89	31.62699762925051	35.0	31.0	35.0	23.5	36.0
90-91	31.38036889119922	34.0	31.0	35.0	23.0	36.0
92-93	31.476107757908217	34.0	31.0	35.0	23.5	36.0
94-95	31.594176055199924	35.0	31.0	35.0	24.0	35.0
96-97	31.412535159517965	34.5	31.0	35.0	23.5	35.0
98-99	31.123947977035638	34.0	31.0	35.0	20.5	35.0
100	29.52488204957256	34.0	27.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23405028896742053
1101	2	0.2754530511031277
1101	3	0.2960117991928186
1101	4	0.29860037035128784
1101	5	0.2522383663452388
1101	6	0.28015234141797407
1101	7	0.36128452550937595
1101	8	0.3422670622309312
1101	9	0.4181326695393892
1101	10-11	0.4585857387630057
1101	12-13	0.4412959352009196
1101	14-15	0.578086752330158
1101	16-17	0.5553467417062663
1101	18-19	0.5384872581228777
1101	20-21	0.5679739288201944
1101	22-23	0.5344552054528222
1101	24-25	0.5815936734415885
1101	26-27	0.5282363055822401
1101	28-29	0.5726086743535319
1101	30-31	0.6068654070325934
1101	32-33	0.62779721858832
1101	34-35	0.5901133741342335
1101	36-37	0.5731472883606514
1101	38-39	0.5928975631696645
1101	40-41	0.5833588727882599
1101	42-43	0.6221303353757293
1101	44-45	0.5554020494719936
1101	46-47	0.5724408423072944
1101	48-49	0.5749082753938168
1101	50-51	0.6716858779884021
1101	52-53	0.589490744188133
1101	54-55	0.5516672567053433
1101	56-57	0.6378211302536343
1101	58-59	0.6317146803750973
1101	60-61	0.6608609815859836
1101	62-63	0.5723088570271884
1101	64-65	0.6516029800755234
1101	66-67	0.5810243640930892
1101	68-69	0.5547469125886337
1101	70-71	0.5496860334270437
1101	72-73	0.5674558729762751
1101	74-75	0.5435851545464061
1101	76-77	0.5700635122706501
1101	78-79	0.5805186559536182
1101	80-81	0.5697014783754781
1101	82-83	0.5452726025007735
1101	84-85	0.5210057147056375
1101	86-87	0.6059847095653481
1101	88-89	0.5195019943290724
1101	90-91	0.5286099131828159
1101	92-93	0.5136836213434854
1101	94-95	0.5277428109252362
1101	96-97	0.549431209528052
1101	98-99	0.5451670897302492
1101	100	0.5400414243303224
1102	1	0.21842390527363875
1102	2	0.2587956828586755
1102	3	0.215349320880037
1102	4	0.2552883742584484
1102	5	0.2236111143134778
1102	6	0.31599979100572284
1102	7	0.2213887018050329
1102	8	0.28088325199104247
1102	9	0.3019952547506648
1102	10-11	0.3442527213212756
1102	12-13	0.3488551521391159
1102	14-15	0.45122838713471936
1102	16-17	0.4203523328906158
1102	18-19	0.4276457587974818
1102	20-21	0.38293409845543636
1102	22-23	0.4322246182899079
1102	24-25	0.42956246859170477
1102	26-27	0.43937640071850126
1102	28-29	0.5028152812919373
1102	30-31	0.4639095805429676
1102	32-33	0.45431078397339064
1102	34-35	0.4755772380271637
1102	36-37	0.48453003410405415
1102	38-39	0.49604469999561474
1102	40-41	0.47771490640281655
1102	42-43	0.5414228358373094
1102	44-45	0.46228452263152064
1102	46-47	0.45765233561812835
1102	48-49	0.42177951705404837
1102	50-51	0.466918107447988
1102	52-53	0.4641531532328287
1102	54-55	0.41075640073371744
1102	56-57	0.4911508176107162
1102	58-59	0.4694171348674132
1102	60-61	0.47472522025445585
1102	62-63	0.3908266284774271
1102	64-65	0.44968815107550597
1102	66-67	0.48960414807370256
1102	68-69	0.468915545692667
1102	70-71	0.45211821378028816
1102	72-73	0.4087619816454122
1102	74-75	0.4112380817479462
1102	76-77	0.4517224381372955
1102	78-79	0.3759286199317913
1102	80-81	0.30464725395609094
1102	82-83	0.3900380517716542
1102	84-85	0.39890454180641655
1102	86-87	0.37859819338148526
1102	88-89	0.35332446530986417
1102	90-91	0.3789986939843679
1102	92-93	0.4599198165528513
1102	94-95	0.5064673476236159
1102	96-97	0.4966140484133845
1102	98-99	0.5183067957035803
1102	100	0.457732312507364
1103	1	0.16639104081106737
1103	2	0.2071487896919173
1103	3	0.20607402884544257
1103	4	0.24610379501822877
1103	5	0.2211400113749704
1103	6	0.2326624827329482
1103	7	0.2042259140619791
1103	8	0.2801281163786413
1103	9	0.35621299845382737
1103	10-11	0.349452447157077
1103	12-13	0.37982007532813356
1103	14-15	0.4962617480064324
1103	16-17	0.476842371767205
1103	18-19	0.46970281128093916
1103	20-21	0.3608126345568863
1103	22-23	0.4703422719705159
1103	24-25	0.5039381496391258
1103	26-27	0.4664371383916972
1103	28-29	0.4495426846591002
1103	30-31	0.48228045058557
1103	32-33	0.5037292793273238
1103	34-35	0.4996470909753583
1103	36-37	0.5103155737944007
1103	38-39	0.5219693251550837
1103	40-41	0.501383510258556
1103	42-43	0.5514088470433833
1103	44-45	0.5122261387210543
1103	46-47	0.5709040887908898
1103	48-49	0.41837686133808916
1103	50-51	0.4999818968645613
1103	52-53	0.5550225695699282
1103	54-55	0.4481112308014161
1103	56-57	0.5063754015018986
1103	58-59	0.4933428658643919
1103	60-61	0.5289797344856169
1103	62-63	0.5222543300155564
1103	64-65	0.43792623980939993
1103	66-67	0.5626832366112993
1103	68-69	0.4965268311835942
1103	70-71	0.47012812658713443
1103	72-73	0.43398022265558467
1103	74-75	0.49605966514159405
1103	76-77	0.4493340513502915
1103	78-79	0.441220156767578
1103	80-81	0.3566451015404084
1103	82-83	0.4249163776595992
1103	84-85	0.38036114068661675
1103	86-87	0.3615023810297515
1103	88-89	0.3857248840982628
1103	90-91	0.42464118860473477
1103	92-93	0.404681757336391
1103	94-95	0.467365040651071
1103	96-97	0.5188643985541077
1103	98-99	0.35496098517564434
1103	100	0.5428914243760765
1104	1	0.06557202551285002
1104	2	0.08251763646090637
1104	3	0.08943203447327974
1104	4	0.13470068441689165
1104	5	0.16672957103391894
1104	6	0.18160920531859404
1104	7	0.22468501456322088
1104	8	0.2182272620381056
1104	9	0.3038738867136317
1104	10-11	0.3174501012355222
1104	12-13	0.24987377167276037
1104	14-15	0.3800619517068782
1104	16-17	0.38195760619763774
1104	18-19	0.301508162059136
1104	20-21	0.3513704213024482
1104	22-23	0.4060070179533213
1104	24-25	0.3980136965862684
1104	26-27	0.40603007083544185
1104	28-29	0.3471665424745183
1104	30-31	0.3610995314516572
1104	32-33	0.3659230548242576
1104	34-35	0.3817874429604018
1104	36-37	0.45916815258090793
1104	38-39	0.42654208447765996
1104	40-41	0.422395020059966
1104	42-43	0.43029635612222705
1104	44-45	0.4522541458824705
1104	46-47	0.39034153517320647
1104	48-49	0.4378476307198298
1104	50-51	0.4383292323117587
1104	52-53	0.43623438261172964
1104	54-55	0.4260115859020317
1104	56-57	0.5087982497921502
1104	58-59	0.48086428818550075
1104	60-61	0.4969356110499277
1104	62-63	0.4356647250311667
1104	64-65	0.5311438927921159
1104	66-67	0.4748532877608369
1104	68-69	0.4273900233839214
1104	70-71	0.46250176377107266
1104	72-73	0.4505014555339528
1104	74-75	0.4033180667949594
1104	76-77	0.37835507245324607
1104	78-79	0.46948697685132856
1104	80-81	0.374886390859956
1104	82-83	0.3651779428526538
1104	84-85	0.3526298923821969
1104	86-87	0.3903440476065754
1104	88-89	0.37199190836651397
1104	90-91	0.38438760486961243
1104	92-93	0.32264084078558497
1104	94-95	0.36945342679917204
1104	96-97	0.403574658979565
1104	98-99	0.3857540934645023
1104	100	0.49806362914409164
1105	1	0.15328983416054598
1105	2	0.15640467942581893
1105	3	0.173610099877628
1105	4	0.2537335222506911
1105	5	0.19605294336624013
1105	6	0.25571021841798824
1105	7	0.21562403219016346
1105	8	0.25681931335190455
1105	9	0.27422691840046554
1105	10-11	0.37990574754033446
1105	12-13	0.3172220042193956
1105	14-15	0.4362640989793505
1105	16-17	0.37434610325328777
1105	18-19	0.37760686424890366
1105	20-21	0.337486062548777
1105	22-23	0.40316390420181136
1105	24-25	0.38791763603354923
1105	26-27	0.35246347475577977
1105	28-29	0.3184144363526258
1105	30-31	0.4013837457748224
1105	32-33	0.4367224505710894
1105	34-35	0.4158902635859789
1105	36-37	0.4119922822136175
1105	38-39	0.3583586954770581
1105	40-41	0.3777852095199137
1105	42-43	0.29821589777519364
1105	44-45	0.35083594829225007
1105	46-47	0.4171197058119347
1105	48-49	0.3988849721946579
1105	50-51	0.39597868204577225
1105	52-53	0.42703943096640273
1105	54-55	0.3589143614225634
1105	56-57	0.4360751603904873
1105	58-59	0.3815568372043856
1105	60-61	0.40327990998848406
1105	62-63	0.354976455951153
1105	64-65	0.44383374269344245
1105	66-67	0.3714500419671438
1105	68-69	0.3310470413496347
1105	70-71	0.3744223426470157
1105	72-73	0.3394943476289285
1105	74-75	0.37717082788906
1105	76-77	0.4228063290056596
1105	78-79	0.44141629898503965
1105	80-81	0.362808204387278
1105	82-83	0.38148078611654057
1105	84-85	0.3802353909722882
1105	86-87	0.3118695155218063
1105	88-89	0.32027967543574576
1105	90-91	0.376638285426548
1105	92-93	0.37901241347653425
1105	94-95	0.3942983058244103
1105	96-97	0.38008523692198537
1105	98-99	0.44025824656556267
1105	100	0.5183743306159947
1106	1	0.013175292924529458
1106	2	0.12609851218552137
1106	3	0.0724515086731472
1106	4	0.02608588733068018
1106	5	-0.01743118109710906
1106	6	0.05321301860376337
1106	7	0.09252692220621128
1106	8	0.11364687238012294
1106	9	0.16142118578162723
1106	10-11	0.16351437512047085
1106	12-13	0.1543564314902781
1106	14-15	0.1813881687492227
1106	16-17	0.20955439272087517
1106	18-19	0.2498266379194547
1106	20-21	0.30030515244888534
1106	22-23	0.27937535098400446
1106	24-25	0.263618447207989
1106	26-27	0.2394397304039444
1106	28-29	0.21556185383445126
1106	30-31	0.2180849994729286
1106	32-33	0.2649887901713015
1106	34-35	0.2683842642803427
1106	36-37	0.2027153189864208
1106	38-39	0.21961867966244597
1106	40-41	0.2377123715513818
1106	42-43	0.1928945888839877
1106	44-45	0.22635794013819321
1106	46-47	0.25007039807088205
1106	48-49	0.26097393627631504
1106	50-51	0.2504512937562353
1106	52-53	0.20666804756501733
1106	54-55	0.14711307596267886
1106	56-57	0.25284689668920635
1106	58-59	0.2249987307366652
1106	60-61	0.24673308328595311
1106	62-63	0.25039387608914865
1106	64-65	0.26643654405180683
1106	66-67	0.27620296540187894
1106	68-69	0.2217123410192272
1106	70-71	0.17761525543672008
1106	72-73	0.2009685198980975
1106	74-75	0.2785280704159945
1106	76-77	0.2408512257849722
1106	78-79	0.18557855528475642
1106	80-81	0.1885519041604553
1106	82-83	0.2220308027621627
1106	84-85	0.2506061370988064
1106	86-87	0.20399213536389027
1106	88-89	0.17410439527696653
1106	90-91	0.18403691921118792
1106	92-93	0.22573369799834708
1106	94-95	0.17857821326151324
1106	96-97	0.111886833372008
1106	98-99	0.23454988240936814
1106	100	0.3520732982816881
1107	1	0.07583410277124258
1107	2	0.12734333590005065
1107	3	0.15864445886410294
1107	4	0.13079303734537007
1107	5	0.17418303522634915
1107	6	0.1837583344929996
1107	7	0.1830985370462983
1107	8	0.1778273793643379
1107	9	0.20674743764790549
1107	10-11	0.22834078594750906
1107	12-13	0.2588840463947619
1107	14-15	0.29649960225332705
1107	16-17	0.2797304870349038
1107	18-19	0.33791987343491314
1107	20-21	0.28747661677930125
1107	22-23	0.3037697406752997
1107	24-25	0.3596423154677524
1107	26-27	0.3107359447232909
1107	28-29	0.2636983407614366
1107	30-31	0.3148442624584149
1107	32-33	0.33041874884150246
1107	34-35	0.2904468606125832
1107	36-37	0.3272639408570086
1107	38-39	0.3464253994770061
1107	40-41	0.2715380137327301
1107	42-43	0.32794392708406406
1107	44-45	0.32421237232633615
1107	46-47	0.3589228020002295
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2216	86-87	-1.4617229931075642
2216	88-89	-1.3836547618486854
2216	90-91	-1.3967202812080721
2216	92-93	-1.3496194713375935
2216	94-95	-1.3745405290834007
2216	96-97	-1.461602437078568
2216	98-99	-1.5282726369861592
2216	100	-1.423339909052686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1289.0
1	310.0
2	277.0
3	239.0
4	184.0
5	250.0
6	318.0
7	797.0
8	1974.0
9	2531.0
10	2540.0
11	2973.0
12	4181.0
13	5339.0
14	6942.0
15	8195.0
16	8860.0
17	9895.0
18	10969.0
19	12478.0
20	14433.0
21	16519.0
22	18803.0
23	22652.0
24	27681.0
25	33902.0
26	38385.0
27	41844.0
28	47942.0
29	59183.0
30	71697.0
31	87155.0
32	113429.0
33	154778.0
34	228511.0
35	356631.0
36	598610.0
37	976661.0
38	1037981.0
39	315643.0
40	793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.682135767411786	16.326532963809154	12.164869054132096	21.826462214646966
2	12.635496906844104	21.995317072091932	40.69553735031241	24.673648670751557
3	23.273857471755914	27.067016219265145	30.90429914673283	18.75482716224611
4	11.537203991473637	14.492698189449605	42.002847497586956	31.967250321489804
5	13.267477942530185	40.82035745877855	34.23483373975469	11.677330858936573
6	26.04490590011238	37.777545298669466	20.22279435638018	15.954754444837969
7	26.063964607479434	36.25636535404041	20.028226456830804	17.65144358164935
8	20.200855284790386	32.831824944738756	21.93974387540926	25.027575895061595
9	24.596394610577303	21.932716538317457	21.721151147954775	31.749737703150466
10-11	22.522456514336085	23.635178930944114	29.662747922544895	24.1796166321749
12-13	31.91006372085662	20.92572058891627	25.82864874416303	21.33556694606408
14-15	27.271335439968393	23.40106744880197	28.57507220197892	20.752524909250724
16-17	20.89258129553291	26.68513894007382	31.408367612679466	21.013912151713797
18-19	19.44032599246629	32.91361987621374	28.583139622088545	19.062914509231437
20-21	21.147595500968926	30.531374121713164	23.677808038407385	24.64322233891053
22-23	20.453330138569058	28.731889776024754	21.887207157807918	28.927572927598273
24-25	22.99200726582874	28.38677451439607	25.257821030122912	23.363397189652275
26-27	26.669504999970627	24.027320801124183	27.03788933057379	22.2652848683314
28-29	22.100238905435702	25.041870084196365	30.61348860689031	22.24440240347762
30-31	21.126809728783048	31.77682650854558	27.22751203644106	19.86885172623031
32-33	21.081175427611246	30.265169952896947	24.544427617583374	24.109227001908433
34-35	22.849340124471425	26.177664476932094	25.283605747313025	25.689389651283456
36-37	25.978159045843373	25.801126276808233	27.651364112026744	20.56935056532165
38-39	28.471427819258587	21.977397933948712	29.16995837043641	20.38121587635629
40-41	22.37851688922499	25.247650568458056	32.38755839304551	19.98627414927145
42-43	20.457135154254456	30.13915564163332	28.02916991666561	21.374539287446613
44-45	20.75646925559781	31.507678035868008	24.537052825034237	23.198799883499944
46-47	20.874554931165683	27.72313694353003	22.185285960398236	29.217022164906055
48-49	23.36550796365883	26.987089514801998	24.545430035733286	25.101972485805884
50-51	27.385078891429952	27.790095222733633	24.917778942699304	19.907046943137107
52-53	21.73154651623667	27.571182331849315	29.773640645595407	20.923630506318613
54-55	19.39820269114754	26.96644164783189	30.662443406384384	22.97291225463619
56-57	22.705617301247287	31.032379465322663	24.478047148350658	21.7839560850794
58-59	20.74111320571449	27.90597029560255	26.289846993582266	25.063069505100692
60-61	21.760472584107323	24.51222420783746	28.40800129967543	25.319301908379792
62-63	28.926704345649902	23.48379961958453	25.438901929407905	22.150594105357662
64-65	25.825859596839944	24.86729787266403	29.25484477411487	20.051997756381155
66-67	21.338708826238566	25.812632523552637	30.780561852620792	22.068096797588012
68-69	20.152920459537977	31.217387106255856	28.02443307560839	20.605259358597774
70-71	20.878888403664607	29.78194865815002	24.15936045647442	25.17980248171095
72-73	20.76323075100807	27.96751403511117	23.017547748874634	28.251707465006128
74-75	22.74602106840907	28.125687756046897	25.572310973239187	23.555980202304845
76-77	25.72386690539099	24.219760801732107	27.085915983218626	22.970456309658278
78-79	22.196854929416304	24.658460508126094	30.311390080913508	22.833294481544094
80-81	21.242910355366295	30.845915605358833	27.359636184262605	20.551537855012267
82-83	21.39082194319048	29.102272067316687	25.19487768521446	24.312028304278364
84-85	22.60526244531849	25.58182623709983	25.547523726287398	26.26538759129428
86-87	25.096108832357046	25.74099002380814	27.493031137475754	21.669870006359055
88-89	27.498283220263993	22.164663372907604	28.584855007005384	21.752198399823023
90-91	22.492370606804936	24.709025324999594	31.28689537217359	21.511708696021884
92-93	20.981452494296292	29.35223255135526	28.000889918960226	21.665425035388218
94-95	20.98735149102903	30.508712426444006	25.150130618996013	23.35380546353095
96-97	21.084055426325776	27.059540556452287	23.13501842820489	28.721385589017046
98-99	23.057022222744592	26.889799808858903	24.64126476682469	25.411913201571814
100	21.803640217227752	31.601228483310518	26.344611354147148	20.250519945314586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1969.0
1	1218.5
2	481.0
3	497.0
4	436.0
5	353.5
6	312.0
7	308.0
8	336.0
9	344.5
10	330.5
11	328.5
12	354.0
13	373.5
14	397.5
15	475.0
16	553.0
17	656.5
18	890.5
19	1219.5
20	1688.5
21	2415.0
22	3447.5
23	4985.5
24	7195.5
25	9767.0
26	13132.5
27	17635.5
28	22472.5
29	28700.0
30	35849.0
31	42100.5
32	48967.0
33	56088.0
34	63006.5
35	71151.5
36	79660.5
37	87422.0
38	91465.0
39	92654.5
40	94166.5
41	95094.0
42	97323.5
43	103376.0
44	115904.0
45	130453.5
46	160750.5
47	240460.0
48	681019.0
49	692925.5
50	254865.5
51	160261.0
52	116877.5
53	97202.5
54	85265.0
55	73640.5
56	64635.0
57	56465.5
58	48231.5
59	42225.5
60	34916.0
61	26544.5
62	20123.5
63	15565.5
64	11468.5
65	7791.5
66	5548.5
67	4140.0
68	3175.5
69	2498.5
70	2069.5
71	1748.5
72	1428.0
73	1179.0
74	966.0
75	738.0
76	531.0
77	392.5
78	300.0
79	220.0
80	153.5
81	105.5
82	78.0
83	59.5
84	44.5
85	30.5
86	19.5
87	18.0
88	18.5
89	15.0
90	10.0
91	10.0
92	10.5
93	10.0
94	7.5
95	5.0
96	6.0
97	6.0
98	6.5
99	7.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10359654991258754
2	0.06676222105477864
3	0.08633045826048961
4	0.08801102451462715
5	0.0888858398250001
6	0.0938584742208043
7	0.0891851187469698
8	0.09054338462360151
9	0.0753262025142192
10-11	0.08306141157435908
12-13	0.07189600563933575
14-15	0.08101250203164345
16-17	0.08231321426943483
18-19	0.08174918860879964
20-21	0.07252909566657934
22-23	0.07686864003513995
24-25	0.07958517178840335
26-27	0.0736341255323136
28-29	0.0820254460752332
30-31	0.08574341114431829
32-33	0.08797649233132294
34-35	0.08984123022974952
36-37	0.08977216586314113
38-39	0.09092323863994765
40-41	0.08720527357086258
42-43	0.09275344435507003
44-45	0.08890886128053624
46-47	0.08552470731672504
48-49	0.09491746117546632
50-51	0.0909117279121796
52-53	0.0970124136292542
54-55	0.0884254107142775
56-57	0.09461818225349662
58-59	0.09934909136617145
60-61	0.09735773546229615
62-63	0.09917643044965047
64-65	0.10028146031538472
66-67	0.10246849859131713
68-69	0.09964837028814114
70-71	0.10196202656952227
72-73	0.09899225880536142
74-75	0.10315914225740105
76-77	0.09944117718831598
78-79	0.1066929356821971
80-81	0.10208864457497098
82-83	0.10686559659871808
84-85	0.1022497947637239
86-87	0.09881959788884044
88-89	0.09793327185069942
90-91	0.09915340899411433
92-93	0.09330595928793718
94-95	0.09330595928793718
96-97	0.0911419424675409
98-99	0.10523107325565281
100	0.24462598652692336
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4343774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.405315038704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3682991531894	39.1825713012996
2	14.985890921971146	16.00652451647047
3	5.538920609768075	8.874234004171038
4	2.5424120412807385	5.431132640911695
5	1.332673411430711	3.5585921690580764
6	0.755696146260975	2.4214914458761148
7	0.441538900918771	1.6506366873787057
8	0.29313057405138954	1.2523784523752488
9	0.18684636896910856	0.8980730278769854
>10	0.5496918454639259	4.1422332508587685
>50	0.00314197174918926	0.11203747750659473
>100	9.37385633363695E-4	0.08761754378729483
>500	5.471128754929785E-5	0.026559830548987286
>1k	2.1884515019719077E-4	0.34898857654246773
>5k	5.471128754929769E-5	0.27240627085611413
>10k+	4.924015879436792E-4	15.734522804481852
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	200081	4.6061558451245395	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	161355	3.714626958032347	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	38933	0.8962943283881712	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	38199	0.8793965800246514	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	28382	0.6533949510264576	No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG	27250	0.6273346633595579	No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA	23507	0.5411653552878212	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10708	0.24651374588088606	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	10093	0.23235555072616576	No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG	9323	0.21462902996334526	No Hit
GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	4431	0.10200806948059453	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3021455536130564E-5
2	0.0	0.0	0.0	0.0	2.3021455536130564E-5
3	0.0	0.0	0.0	0.0	2.3021455536130564E-5
4	0.0	0.0	0.0	0.0	2.3021455536130564E-5
5	0.0	0.0	0.0	0.0	2.3021455536130564E-5
6	0.0	0.0	0.0	0.0	4.604291107226113E-5
7	0.0	0.0	0.0	2.3021455536130564E-5	4.604291107226113E-5
8	0.0	0.0	0.0	4.604291107226113E-5	4.604291107226113E-5
9	0.0	0.0	0.0	1.8417164428904452E-4	4.604291107226113E-5
10-11	0.0	0.0	0.0	2.992789219696973E-4	8.057509437645697E-5
12-13	0.0	0.0	0.0	4.1438619965035017E-4	2.1870382759324035E-4
14-15	0.0	0.0	0.0	5.29493477331003E-4	2.8776819420163203E-4
16-17	0.0	0.0	0.0	8.748153103729614E-4	4.2589692741841544E-4
18-19	0.0	0.0	0.0	0.0012776907822552462	4.374076551864807E-4
20-21	0.0	0.0	0.0	0.002117973909324012	5.640256606351988E-4
22-23	0.0	0.0	0.0	0.0034186861471153887	6.215792994755252E-4
24-25	0.0	0.0	0.0	0.004915080756963875	6.215792994755252E-4
26-27	0.0	0.0	0.0	0.006825861566462712	6.215792994755252E-4
28-29	0.0	0.0	0.0	0.011810006690034978	6.215792994755252E-4
30-31	0.0	0.0	0.0	0.025047343623310053	6.215792994755252E-4
32-33	0.0	0.0	0.0	0.05495221436474365	6.906436660839169E-4
34-35	0.0	0.0	0.0	0.12863238280812953	6.906436660839169E-4
36-37	0.0	0.0	0.0	0.23861738663199328	6.906436660839169E-4
38-39	0.0	0.0	0.0	0.344884425386772	7.136651216200474E-4
40-41	0.0	0.0	0.0	0.45295864840113687	7.136651216200474E-4
42-43	0.0	0.0	0.0	0.5598127342720869	7.136651216200474E-4
44-45	0.0	0.0	0.0	0.685532902954896	7.597080326923085E-4
46-47	0.0	0.0	0.0	0.8591031669695524	8.402831270687655E-4
48-49	0.0	0.0	0.0	1.0703480429690864	8.863260381410267E-4
50-51	0.0	0.0	0.0	1.2582261415994478	9.208582214452225E-4
52-53	0.0	0.0	0.0	1.4265475137518666	0.0010359654991258754
54-55	0.0	0.0	0.0	1.6118126771788772	0.0011280513212703976
56-57	0.0	0.0	0.0	1.8192590130149497	0.0012201371434149197
58-59	0.0	0.0	0.0	2.1597233189387843	0.0012892015100233116
60-61	0.0	0.0	0.0	2.6517033344736625	0.0012892015100233116
62-63	0.0	0.0	0.0	3.0314192220866003	0.0014043087877039643
64-65	0.0	0.0	0.0	3.329742753651548	0.0014043087877039643
66-67	0.0	0.0	0.0	3.658592735257405	0.0014043087877039643
68-69	0.0	0.0	0.0	4.003062774444527	0.0014043087877039643
70-71	0.0	0.0	0.0	4.408643267352307	0.0014273302432400948
72-73	0.0	0.0	0.0	4.878902539588847	0.001519416065384617
74-75	0.0	0.0	0.0	5.331561909068013	0.001599991159761074
76-77	0.0	0.0	0.0	5.745268975780047	0.001680566254137531
78-79	0.0	1.1510727768065282E-5	0.0	6.2005067482792615	0.0016920769819055964
80-81	0.0	4.604291107226113E-5	0.0	6.689735699877572	0.0017496306207459228
82-83	0.0	4.604291107226113E-5	0.0	7.200478201674397	0.001841716442890445
84-85	0.0	4.604291107226113E-5	0.0	8.019328353639024	0.001841716442890445
86-87	0.0	4.604291107226113E-5	0.0	8.903985336253683	0.001991355903875294
88	0.0	4.604291107226113E-5	0.0	9.386883387579557	0.002117973909324012
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTC	10085	0.0	22.235775	4
ATACTCT	10310	0.0	21.932402	5
GCTTATA	10680	0.0	20.959248	1
GTACTAG	1115	0.0	20.244465	1
TTATACT	11150	0.0	20.028046	3
GTACCAG	3030	0.0	19.710655	1
GTACCCG	510	0.0	19.363733	1
GCATCGT	905	0.0	19.213116	8
TCGCCGG	275	3.1634045E-8	18.823723	94
CTCGTCG	690	0.0	18.388414	9
GCTATTG	1520	0.0	17.93201	8
CGACGAG	505	0.0	17.680593	24-25
CATGGGG	4490	0.0	17.38087	4
TGATCGC	585	0.0	16.869143	9
GGCGTCG	615	0.0	16.810947	8
CTGTGCG	1455	0.0	16.471638	9
CCTACAC	1665	0.0	16.37697	3
CTAACGC	460	1.2732926E-11	16.352417	3
TACCTGG	3350	0.0	16.279198	2
CTAGTAC	1710	0.0	16.220928	3
>>END_MODULE