FastQCFastQC Report
Mon 16 Jan 2017
ERR1146599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4343774
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1947914.484372345338408No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1574043.623669187209095No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG384150.8843692144204557No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG362320.8341133769850826No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG279810.6441633473564693No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT278510.6411705581367724No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA244290.5623911372921335No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113710.26177697090134067No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC111510.25671225068339193No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99400.2288332680291378No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT74590.17171703684399786No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC73070.16821777560250603No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG60470.13921074162698152No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA60140.1384510335942892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58070.13368559229831017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55990.12889712954679503No Hit
GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45220.1041030219343824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG5750.026.9876291
GAGTACT79400.021.30863412-13
GTACCAG26300.020.9193741
CTAACGC3900.020.4966533
TATACTC102550.020.3585074
ATACTCT105300.019.8630185
GCTTATA107850.019.4461361
AGTACTT85100.018.72163412-13
TTATACT111600.018.7075713
GTACTTT89900.018.68916914-15
ACTGCGC7600.017.9332088
GCATCGT10200.017.5088278
GTACTAG12450.017.3742921
CGACGAG4750.017.31492224-25
TAACGCC4900.017.2732894
TACCTGG31200.016.880182
TCTAACG4751.8189894E-1216.8294122
ACCGTGC4308.0035534E-1116.3944348
GCTTATG74200.016.3506281
TGATCGC5200.016.2683249
Produced by FastQC (version 0.11.5)