##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146599_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4343774
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.420434166234244	33.0	31.0	34.0	31.0	34.0
2	32.47380480660366	34.0	31.0	34.0	31.0	34.0
3	32.62756579877314	34.0	31.0	34.0	31.0	34.0
4	35.98568848195141	37.0	35.0	37.0	35.0	37.0
5	36.06418519932206	37.0	35.0	37.0	35.0	37.0
6	36.158093630101384	37.0	36.0	37.0	35.0	37.0
7	36.165395345153776	37.0	36.0	37.0	35.0	37.0
8	36.1763312271771	37.0	37.0	37.0	35.0	37.0
9	37.980057894356385	39.0	38.0	39.0	35.0	39.0
10-11	37.93683119793985	39.0	38.0	39.0	35.0	39.0
12-13	37.92433250440746	39.0	38.0	39.0	35.0	39.0
14-15	39.14877097657475	40.0	39.0	41.0	36.0	41.0
16-17	39.1031503941043	40.0	38.5	41.0	36.0	41.0
18-19	39.33475751731098	40.5	39.0	41.0	36.0	41.0
20-21	39.39460697080465	41.0	39.0	41.0	36.0	41.0
22-23	39.385915335374264	41.0	39.0	41.0	36.0	41.0
24-25	39.3748424756905	41.0	39.0	41.0	36.0	41.0
26-27	39.12406319942059	40.5	38.5	41.0	35.5	41.0
28-29	38.99620663966404	40.0	38.5	41.0	35.5	41.0
30-31	39.02390559453599	40.0	38.5	41.0	36.0	41.0
32-33	39.03825647927356	40.0	39.0	41.0	35.0	41.0
34-35	39.02662511907848	40.0	39.0	41.0	35.0	41.0
36-37	38.89875290473215	40.0	38.0	41.0	35.0	41.0
38-39	38.69849628456729	40.0	38.0	41.0	35.0	41.0
40-41	38.56550340786606	40.0	38.0	41.0	35.0	41.0
42-43	38.66096935521968	40.0	38.0	41.0	35.0	41.0
44-45	38.673895096752275	40.0	38.0	41.0	35.0	41.0
46-47	38.53258203580573	40.0	38.0	41.0	34.5	41.0
48-49	38.3270277182929	40.0	38.0	41.0	34.0	41.0
50-51	38.2349494932287	40.0	38.0	41.0	34.0	41.0
52-53	37.868091433854524	40.0	37.0	41.0	32.5	41.0
54-55	37.773972471864326	40.0	37.0	41.0	32.5	41.0
56-57	37.8563550958222	40.0	37.0	41.0	33.0	41.0
58-59	37.776141668512224	40.0	37.0	41.0	33.0	41.0
60-61	37.5032558093492	39.5	36.5	41.0	32.5	41.0
62-63	37.118838364979396	39.0	36.0	41.0	32.0	41.0
64-65	36.639068353924486	39.0	35.0	40.5	31.0	41.0
66-67	36.38184583267914	38.0	35.0	40.0	31.0	41.0
68-69	36.14197711483148	37.0	35.0	39.0	31.0	41.0
70-71	35.73504767973656	37.0	35.0	39.0	31.0	41.0
72-73	35.23469372485769	36.0	35.0	38.5	30.5	40.5
74-75	34.85972658798548	35.5	34.0	37.0	30.0	39.5
76-77	34.31385035225129	35.0	34.0	37.0	29.5	39.0
78-79	33.97668835901683	35.0	34.0	36.0	29.0	39.0
80-81	33.882165946018375	35.0	34.0	36.0	29.0	38.0
82-83	33.689288277889226	35.0	34.0	36.0	29.0	37.0
84-85	33.52344424456705	35.0	34.0	35.5	29.0	37.0
86-87	33.22316193245781	35.0	34.0	35.0	29.0	36.5
88-89	32.83711364817783	35.0	33.5	35.0	27.5	36.0
90-91	32.670700754689356	35.0	33.0	35.0	27.0	36.0
92-93	32.79089186039605	35.0	33.0	35.0	28.0	36.0
94-95	32.8679306289876	35.0	33.5	35.0	29.0	36.0
96-97	32.771633376874576	35.0	33.0	35.0	29.0	35.0
98-99	32.645837237388506	35.0	33.0	35.0	27.0	35.0
100	31.575866976504763	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32047410842034196
1101	2	0.34447920395366793
1101	3	0.3786761823839271
1101	4	0.2608573266921397
1101	5	0.22389544996846666
1101	6	0.27357216572838894
1101	7	0.2591636275350595
1101	8	0.2693806942824537
1101	9	0.34121656821174895
1101	10-11	0.3212796475283639
1101	12-13	0.33481983646534275
1101	14-15	0.5208929098256831
1101	16-17	0.5185075530110268
1101	18-19	0.4718210730078667
1101	20-21	0.4475257524476106
1101	22-23	0.438748884044422
1101	24-25	0.44208144946340155
1101	26-27	0.4914141684531046
1101	28-29	0.4964771449084111
1101	30-31	0.5191443988836895
1101	32-33	0.539969022519081
1101	34-35	0.47238168266947866
1101	36-37	0.499282689796523
1101	38-39	0.5430592907780607
1101	40-41	0.5336089948421474
1101	42-43	0.5322364898461842
1101	44-45	0.5043222056986281
1101	46-47	0.49857399231625266
1101	48-49	0.6353715392236055
1101	50-51	0.5741057528394649
1101	52-53	0.5737175614952861
1101	54-55	0.5720354964885743
1101	56-57	0.5192004739165199
1101	58-59	0.5375139554412129
1101	60-61	0.5183534985296703
1101	62-63	0.5471248008010505
1101	64-65	0.5090964608737778
1101	66-67	0.4771767761421728
1101	68-69	0.4756361444283783
1101	70-71	0.430597784933866
1101	72-73	0.4779729883861279
1101	74-75	0.44405561791121784
1101	76-77	0.39983222513419747
1101	78-79	0.36609403929895734
1101	80-81	0.37586975011046775
1101	82-83	0.4085979230568526
1101	84-85	0.39344488227065
1101	86-87	0.3661666428071797
1101	88-89	0.46236072711153753
1101	90-91	0.44598649142053404
1101	92-93	0.4293244719604772
1101	94-95	0.40784940900786637
1101	96-97	0.3719817159350072
1101	98-99	0.4261371348632039
1101	100	0.5186579671630582
1102	1	0.2653307040647732
1102	2	0.2659932418417341
1102	3	0.2511586027250132
1102	4	0.16232941310766336
1102	5	0.17827880383960348
1102	6	0.20272959159515835
1102	7	0.20354759887481322
1102	8	0.18495787458353163
1102	9	0.23398240785866165
1102	10-11	0.25416141599729514
1102	12-13	0.25066873631581643
1102	14-15	0.37217363859392094
1102	16-17	0.4595661556346755
1102	18-19	0.39664181301427703
1102	20-21	0.34697249026024934
1102	22-23	0.3597043948850498
1102	24-25	0.36053866895736064
1102	26-27	0.2995506275605493
1102	28-29	0.37512278755737327
1102	30-31	0.4086561091941121
1102	32-33	0.3801707388392046
1102	34-35	0.39164939037124213
1102	36-37	0.4028456474541784
1102	38-39	0.4249832489726444
1102	40-41	0.42154101967579294
1102	42-43	0.3887776811893957
1102	44-45	0.39944275097801096
1102	46-47	0.4130250050982127
1102	48-49	0.4898937810738033
1102	50-51	0.5380056445580337
1102	52-53	0.5473286483443687
1102	54-55	0.48896140292400503
1102	56-57	0.4766578418940881
1102	58-59	0.40455896720466455
1102	60-61	0.3711026713764838
1102	62-63	0.43966606505165373
1102	64-65	0.4785298842920227
1102	66-67	0.4383575314045487
1102	68-69	0.3962055099302546
1102	70-71	0.3577068132711858
1102	72-73	0.3363858738763952
1102	74-75	0.3568794371329034
1102	76-77	0.35860611140400067
1102	78-79	0.35275609557375276
1102	80-81	0.3278093567065383
1102	82-83	0.3286697501840763
1102	84-85	0.30311307598935144
1102	86-87	0.4120942978069664
1102	88-89	0.4467788642390573
1102	90-91	0.3936030747107395
1102	92-93	0.33260814334877153
1102	94-95	0.41296774159029326
1102	96-97	0.3380622938904807
1102	98-99	0.37871991265674865
1102	100	0.49734071251828027
1103	1	0.1935893977623735
1103	2	0.21676520688007628
1103	3	0.22666146249285646
1103	4	0.19675726144731698
1103	5	0.1449882184079101
1103	6	0.16306225867252522
1103	7	0.15062707899538452
1103	8	0.17053661659993935
1103	9	0.18525810528096542
1103	10-11	0.19797585280523577
1103	12-13	0.20883046917541748
1103	14-15	0.3425806026204796
1103	16-17	0.39085686766585326
1103	18-19	0.33230375659243805
1103	20-21	0.295294788974644
1103	22-23	0.27498843732326606
1103	24-25	0.2968934725379242
1103	26-27	0.2993361070936871
1103	28-29	0.3112927985416718
1103	30-31	0.3668568151657965
1103	32-33	0.3195522998040943
1103	34-35	0.2999024617274486
1103	36-37	0.2644842532930909
1103	38-39	0.3835326085734252
1103	40-41	0.3305397429065309
1103	42-43	0.34678328310427986
1103	44-45	0.3557363277714245
1103	46-47	0.34726702548678645
1103	48-49	0.4173556864701027
1103	50-51	0.4082313120644727
1103	52-53	0.4143046345100032
1103	54-55	0.43407232000819107
1103	56-57	0.32703828288003933
1103	58-59	0.2763102959661268
1103	60-61	0.278714888967329
1103	62-63	0.3140727842967621
1103	64-65	0.3148959982120303
1103	66-67	0.3190852741660706
1103	68-69	0.27742994241832974
1103	70-71	0.26404922009121634
1103	72-73	0.27457047769576093
1103	74-75	0.30229287984310815
1103	76-77	0.25667777288416005
1103	78-79	0.2858714450988771
1103	80-81	0.25695242298866106
1103	82-83	0.22105194055491495
1103	84-85	0.26064775981443944
1103	86-87	0.21934348464659337
1103	88-89	0.3047255042405226
1103	90-91	0.23654168552160115
1103	92-93	0.28274186405258206
1103	94-95	0.3074940283230774
1103	96-97	0.33425301589922896
1103	98-99	0.2888004413756917
1103	100	0.337793573907895
1104	1	0.15891285088321894
1104	2	0.15328588309163393
1104	3	0.13625563698568044
1104	4	0.16253583919221626
1104	5	0.07571592934777271
1104	6	0.09874611944949407
1104	7	0.11064063482235298
1104	8	0.06943189701149066
1104	9	0.14810950670616307
1104	10-11	0.14231219705327902
1104	12-13	0.15247114492932212
1104	14-15	0.276922934887196
1104	16-17	0.31026366987025256
1104	18-19	0.25635039316975394
1104	20-21	0.218451776276261
1104	22-23	0.23216332245298332
1104	24-25	0.2064246644715837
1104	26-27	0.26193824610241734
1104	28-29	0.2579473410198574
1104	30-31	0.2843475724064817
1104	32-33	0.2642490748110333
1104	34-35	0.24896197535027653
1104	36-37	0.2719333573345537
1104	38-39	0.2783065193381944
1104	40-41	0.22410128066229618
1104	42-43	0.2428143422519966
1104	44-45	0.20373724484149136
1104	46-47	0.2963650987375388
1104	48-49	0.3468172279443067
1104	50-51	0.3215220127143681
1104	52-53	0.314021713553835
1104	54-55	0.40560225053045684
1104	56-57	0.36354426682823515
1104	58-59	0.3097008434996411
1104	60-61	0.31101924863657615
1104	62-63	0.272342905876755
1104	64-65	0.2815103187627841
1104	66-67	0.2974610238235442
1104	68-69	0.277603763280311
1104	70-71	0.22816635092926418
1104	72-73	0.2644074579301332
1104	74-75	0.21689532292832325
1104	76-77	0.30699662965335506
1104	78-79	0.3368781319214378
1104	80-81	0.3067785581214011
1104	82-83	0.2615333341317694
1104	84-85	0.2808451939104799
1104	86-87	0.2908265715466811
1104	88-89	0.3574516149283298
1104	90-91	0.3134760641857284
1104	92-93	0.32905285729798806
1104	94-95	0.3312152678462468
1104	96-97	0.28756519992755614
1104	98-99	0.3112063905377127
1104	100	0.36742887573682026
1105	1	0.1791814875586084
1105	2	0.170170837186852
1105	3	0.18329284634112497
1105	4	0.1475494846475911
1105	5	0.06995527745013419
1105	6	0.09180317598935517
1105	7	0.11790891690946381
1105	8	0.09969442155666286
1105	9	0.15035982586509533
1105	10-11	0.16689790438449137
1105	12-13	0.1732460337096171
1105	14-15	0.27817692963534313
1105	16-17	0.3298436530110962
1105	18-19	0.22991136827731395
1105	20-21	0.22140726389304888
1105	22-23	0.23119336561859427
1105	24-25	0.2113672646572411
1105	26-27	0.2567787132838504
1105	28-29	0.20733058906451163
1105	30-31	0.2523876703185266
1105	32-33	0.24575020251754864
1105	34-35	0.22745627440411909
1105	36-37	0.21687158428274245
1105	38-39	0.24886696612907144
1105	40-41	0.22917248813214997
1105	42-43	0.2965373877389794
1105	44-45	0.2687845008467704
1105	46-47	0.3249033955845988
1105	48-49	0.36357786434580674
1105	50-51	0.3381511329531435
1105	52-53	0.3756283962853786
1105	54-55	0.3351858884023713
1105	56-57	0.34594379563817057
1105	58-59	0.3072679620514265
1105	60-61	0.3382812984849366
1105	62-63	0.2765105245197219
1105	64-65	0.29562553791339496
1105	66-67	0.3030193813157567
1105	68-69	0.26280743759336644
1105	70-71	0.2586659021576665
1105	72-73	0.28650576579929776
1105	74-75	0.3460175590446468
1105	76-77	0.3242907972741378
1105	78-79	0.32143401104913494
1105	80-81	0.29656631669188016
1105	82-83	0.2749438942134077
1105	84-85	0.24374809168433842
1105	86-87	0.2842788602281914
1105	88-89	0.3260325631733991
1105	90-91	0.33798480017800614
1105	92-93	0.3235749832469068
1105	94-95	0.3414485014591122
1105	96-97	0.3686804173087239
1105	98-99	0.39226511937441444
1105	100	0.3104553126437253
1106	1	0.11221799525153386
1106	2	0.0890217847126138
1106	3	0.13129465824103903
1106	4	0.1442410849059712
1106	5	0.08768801150716854
1106	6	0.12090869188375564
1106	7	0.10494218316749482
1106	8	0.08674652565984786
1106	9	0.09255203612298146
1106	10-11	0.09390436431238669
1106	12-13	0.13796398604860372
1106	14-15	0.22490434474140386
1106	16-17	0.3224486644387099
1106	18-19	0.1989529374621668
1106	20-21	0.19289926966217052
1106	22-23	0.18753569081537336
1106	24-25	0.1776086608092271
1106	26-27	0.22721990377448975
1106	28-29	0.2307763521297943
1106	30-31	0.24598541579298683
1106	32-33	0.2383162509596204
1106	34-35	0.17703637399233685
1106	36-37	0.1780420568399066
1106	38-39	0.20097916270339056
1106	40-41	0.22862789851932064
1106	42-43	0.20842673184038318
1106	44-45	0.20397019254745885
1106	46-47	0.2201477573589159
1106	48-49	0.31343728150950767
1106	50-51	0.24614493048428443
1106	52-53	0.20931814895644152
1106	54-55	0.2106489705593333
1106	56-57	0.24673794209005706
1106	58-59	0.20252366731843097
1106	60-61	0.22657919848580832
1106	62-63	0.18788755060771933
1106	64-65	0.23458770774766435
1106	66-67	0.24769698905727466
1106	68-69	0.1280036264857216
1106	70-71	0.18852788881364546
1106	72-73	0.20213560749512283
1106	74-75	0.1253409209805767
1106	76-77	0.1415362582201567
1106	78-79	0.1331369140146066
1106	80-81	0.13042896203516108
1106	82-83	0.14629770055424274
1106	84-85	0.08743120650036218
1106	86-87	0.1445289421817364
1106	88-89	0.1708609374036385
1106	90-91	0.12144201124904441
1106	92-93	0.1354683931619931
1106	94-95	0.16211127242201684
1106	96-97	0.13898275835069995
1106	98-99	0.09473000935273745
1106	100	0.176872813333258
1107	1	0.09341263565815439
1107	2	0.11949597069268236
1107	3	0.09976609963280936
1107	4	0.09699985893620777
1107	5	0.10046342611305192
1107	6	0.07842602665511578
1107	7	0.08181047512407957
1107	8	0.091265337357747
1107	9	0.10027742839386633
1107	10-11	0.0951319840460556
1107	12-13	0.09137756773631622
1107	14-15	0.2103995276267696
1107	16-17	0.2523118072739976
1107	18-19	0.19162036942093152
1107	20-21	0.12481773486585723
1107	22-23	0.11711904259352224
1107	24-25	0.15476665371836873
1107	26-27	0.1967310290154458
1107	28-29	0.17668834420992852
1107	30-31	0.19605714935060803
1107	32-33	0.18252701079205025
1107	34-35	0.1649868466490716
1107	36-37	0.1513706548864988
1107	38-39	0.19596369924222756
1107	40-41	0.2049322822143722
1107	42-43	0.19860984859806763
1107	44-45	0.21396530299500682
1107	46-47	0.23234392097180034
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2216	56-57	-0.7743094676202915
2216	58-59	-0.8817450166249898
2216	60-61	-0.9447076534094165
2216	62-63	-0.9734114429622025
2216	64-65	-0.9473053051306408
2216	66-67	-0.8608238323966191
2216	68-69	-0.7600379675241271
2216	70-71	-0.7780140092309935
2216	72-73	-0.7616490577945143
2216	74-75	-0.7863126023922433
2216	76-77	-0.8382952528761365
2216	78-79	-0.7686563588903326
2216	80-81	-0.8261385443353717
2216	82-83	-0.8341060150584809
2216	84-85	-0.8333813550656899
2216	86-87	-0.8196387241323961
2216	88-89	-0.8081588284067607
2216	90-91	-0.9357893241301127
2216	92-93	-0.9493395680540573
2216	94-95	-0.8713346444814505
2216	96-97	-0.9055432155267553
2216	98-99	-0.9329310245863809
2216	100	-1.2283393130411078
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	22.0
8	121.0
9	251.0
10	228.0
11	168.0
12	165.0
13	196.0
14	362.0
15	588.0
16	1033.0
17	1989.0
18	3291.0
19	5130.0
20	7378.0
21	11218.0
22	15914.0
23	19261.0
24	20198.0
25	20770.0
26	23533.0
27	28437.0
28	36327.0
29	44875.0
30	55648.0
31	70398.0
32	90037.0
33	118653.0
34	167155.0
35	257377.0
36	496316.0
37	1115805.0
38	1348145.0
39	381404.0
40	1381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.90646392524647	16.324524404643917	12.393646741769212	22.375364928340403
2	12.723468609931027	22.031075930002146	40.08567510179875	25.15978035826807
3	23.11371673480645	26.691325754811434	31.419364804788103	18.775592705594015
4	11.62457466335265	14.758931177058205	41.436018058942395	32.18047610064675
5	13.07347597017726	40.44821596508939	34.52342664547105	11.954881419262305
6	26.28388365250464	37.658545794444166	19.982848091648748	16.074722461402448
7	25.559386837344668	36.24463427425091	20.117160791514475	18.07881809688994
8	20.476071641191798	32.4386855805503	22.08866147699757	24.99658130126034
9	24.412665115634468	21.234415050138427	21.847775689987557	32.50514414423955
10-11	22.511057210185065	23.26968358376084	29.712528650214605	24.506730555839486
12-13	31.922390529525707	20.76815000043741	25.96602631720711	21.343433152829775
14-15	27.19688307873327	22.958321836992653	29.05244714071763	20.79234794355645
16-17	20.777480710550225	26.386238375711553	31.771399459063176	21.064881454675046
18-19	19.294515729201006	32.099596479369986	29.611526214107197	18.994361577321808
20-21	20.686563542586565	29.857595854655088	24.921024714970702	24.53481588778764
22-23	19.954191907774206	28.050538540909358	23.159100818781088	28.83616873253535
24-25	22.562603395112177	27.68882543152567	26.490742842514365	23.257828330847783
26-27	26.212724567699087	23.41517290597725	28.173432594438573	22.19866993188509
28-29	21.790601905163573	24.265673122036276	31.86690882168363	22.076816151116518
30-31	20.9306758556166	30.899705048704256	28.569124778303078	19.600494317376064
32-33	20.73604392505345	29.50764458231775	25.83862492482036	23.91768656780844
34-35	22.336351219413373	25.50578771569114	26.570957157252145	25.58690390764334
36-37	25.58527178852023	25.111029640709777	28.773974695958408	20.529723874811584
38-39	28.13403886972977	21.34985058958409	30.237928605303267	20.27818193538287
40-41	22.15672838483702	24.503352575153663	33.356646520098245	19.983272519911075
42-43	20.3241927411509	29.396580024651374	29.16503022486897	21.114197009328752
44-45	20.66716147767388	30.825547810576403	25.51699615953603	22.99029455221369
46-47	20.699039590917945	27.25399617935924	22.9861176018826	29.060846627840213
48-49	23.1427485472876	26.534743659368782	25.198773139744247	25.12373465359937
50-51	27.15921684691699	27.390260174677593	25.445476675351898	20.00504630305352
52-53	21.610981634235674	27.053280894911012	30.28908240012717	21.046655070726146
54-55	19.338455547550456	26.463527734442945	31.235131687194006	22.962885030812593
56-57	22.645860927933676	30.54659092753425	24.97490946515463	21.83263867937744
58-59	20.495432108272507	27.493428191604774	26.931781307989294	25.07935839213342
60-61	21.552320714737593	24.089392386482825	28.92936008258306	25.42892681619652
62-63	28.545392910647266	23.21475539379122	25.87973458524196	22.360117110319557
64-65	25.545938688559232	24.445370677963798	29.772487273114034	20.236203360362932
66-67	21.231521190738224	25.413369237996275	31.249062584640765	22.10604698662473
68-69	20.010288539547684	30.697062943116276	28.50796838064051	20.78468013669553
70-71	20.495159806502766	29.31191391159648	24.7051543087944	25.487771973106355
72-73	20.295956949456084	27.485666682436854	23.51235030263223	28.70602606547483
74-75	22.361885804848086	27.60434077131409	26.019338105354667	24.01443531848316
76-77	25.186044005934257	23.75051685285648	27.492589089777226	23.57085005143204
78-79	21.697726833221296	24.01404786680541	30.7693750978424	23.518850202130892
80-81	20.827671434805374	30.079193485504447	27.975344223286513	21.117790856403662
82-83	20.817380310022944	28.621778498405636	25.62312042862573	24.937720762945688
84-85	21.828024803922236	25.122391409171136	25.921873699639843	27.12771008726679
86-87	24.325690056618967	25.224666384577098	27.831121048194497	22.618522510609438
88-89	26.662816711919174	21.71589728194883	29.053594869346334	22.567691136785662
90-91	21.701120005451482	24.115518454173543	31.712150737141336	22.47121080323364
92-93	20.127370880351325	28.55985863897307	28.541119163381406	22.7716513172942
94-95	20.073185316663473	29.74002406896778	25.72901289030778	24.457777724060968
96-97	19.93257456699683	26.48524786639414	23.646417972073284	29.93575959453575
98-99	21.830020715873456	26.136567297926998	25.135029578565444	26.898382407634102
100	20.683865994261875	30.759270774559543	27.001657204878494	21.55520602630009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	216.0
1	136.5
2	169.0
3	309.5
4	216.0
5	119.5
6	240.0
7	572.5
8	998.0
9	1214.0
10	1012.5
11	781.5
12	854.5
13	1020.0
14	1201.5
15	1411.5
16	1603.0
17	1891.5
18	2287.5
19	2677.0
20	3236.5
21	4006.0
22	5040.0
23	6463.5
24	8465.5
25	11084.5
26	14431.5
27	18701.0
28	23345.0
29	29009.5
30	35437.5
31	41394.0
32	48108.5
33	55078.0
34	61490.5
35	69413.5
36	77987.0
37	85433.0
38	89890.5
39	91092.5
40	91910.0
41	92832.0
42	94984.0
43	101014.5
44	113779.0
45	127252.5
46	153827.5
47	229921.5
48	688845.5
49	692134.5
50	244444.5
51	177208.5
52	131291.0
53	97786.5
54	84196.5
55	73271.5
56	64307.0
57	56654.0
58	48901.0
59	42538.0
60	35021.0
61	26470.5
62	19794.5
63	15223.0
64	11097.5
65	7537.5
66	5403.0
67	3919.0
68	2888.0
69	2289.0
70	1957.5
71	1621.5
72	1321.5
73	1138.0
74	891.0
75	652.5
76	477.0
77	336.0
78	252.0
79	173.5
80	109.0
81	62.0
82	40.5
83	24.0
84	13.0
85	8.5
86	8.0
87	6.0
88	2.5
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026014244755827535
2	0.0035683256081002375
3	1.6115018875291395E-4
4	0.050946481101456935
5	0.0025093386534382313
6	0.005387020595454551
7	0.0
8	3.6834328857808903E-4
9	0.0
10-11	4.604291107226113E-5
12-13	0.0
14-15	1.1510727768065282E-5
16-17	3.107896497377626E-4
18-19	1.3812873321678338E-4
20-21	2.3021455536130562E-4
22-23	0.0
24-25	0.0
26-27	2.647467386655015E-4
28-29	0.0
30-31	1.3812873321678338E-4
32-33	2.647467386655015E-4
34-35	1.1510727768065281E-4
36-37	3.4532183304195845E-5
38-39	7.82729488228439E-4
40-41	5.410042050990682E-4
42-43	0.0
44-45	6.446007550116558E-4
46-47	0.0
48-49	6.906436660839169E-5
50-51	0.0
52-53	2.8776819420163203E-4
54-55	3.3381110527389317E-4
56-57	1.0359654991258753E-4
58-59	7.021543938519823E-4
60-61	5.985578439393947E-4
62-63	1.4963946098484868E-4
64-65	3.913647441142195E-4
66-67	0.0011165405935023322
68-69	0.00244027428682984
70-71	0.0033611325082750624
72-73	0.0031194072251456914
74-75	0.002808617575407929
76-77	0.00409781908543124
78-79	0.0012431585989510505
80-81	0.0010474762268939406
82-83	7.136651216200475E-4
84-85	5.870471161713294E-4
86-87	0.0
88-89	0.0
90-91	1.1510727768065282E-5
92-93	5.7553638840326406E-5
94-95	1.4963946098484868E-4
96-97	2.8776819420163203E-4
98-99	8.057509437645697E-5
100	0.07673051130192317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4343774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.78319066660654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61571343059774	37.60273333968304
2	15.378206400033173	15.926651882466935
3	5.654351499834121	8.784010854357687
4	2.626334603477178	5.439999421046608
5	1.356423638972093	3.5119971960792085
6	0.7953546811986418	2.47116018624524
7	0.4755126269439274	1.7236492717791436
8	0.3079712384072622	1.275818668661936
9	0.19609231725438808	0.9138857267376611
>10	0.5838783221518706	4.30309890021073
>50	0.005567584083960198	0.19811998762729807
>100	0.0026938606636211396	0.3107382352256877
>500	4.470109136952316E-4	0.15919797229380303
>1k	5.587636416766844E-4	0.6606050351248844
>5k	3.911345491736791E-4	1.3938647609421864
>10k+	5.02887277509016E-4	15.324468561517977
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	194791	4.484372345338408	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	157404	3.623669187209095	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	38415	0.8843692144204557	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	36232	0.8341133769850826	No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG	27981	0.6441633473564693	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	27851	0.6411705581367724	No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA	24429	0.5623911372921335	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11371	0.26177697090134067	No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC	11151	0.25671225068339193	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9940	0.2288332680291378	No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT	7459	0.17171703684399786	No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC	7307	0.16821777560250603	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	6047	0.13921074162698152	No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA	6014	0.1384510335942892	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5807	0.13368559229831017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5599	0.12889712954679503	No Hit
GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	4522	0.1041030219343824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3021455536130564E-5	6.906436660839169E-5
2	0.0	0.0	0.0	2.3021455536130564E-5	6.906436660839169E-5
3	0.0	0.0	0.0	2.3021455536130564E-5	6.906436660839169E-5
4	0.0	0.0	0.0	4.604291107226113E-5	6.906436660839169E-5
5	0.0	0.0	0.0	4.604291107226113E-5	6.906436660839169E-5
6	0.0	0.0	0.0	4.604291107226113E-5	1.1510727768065281E-4
7	0.0	0.0	0.0	4.604291107226113E-5	1.1510727768065281E-4
8	0.0	0.0	0.0	4.604291107226113E-5	1.1510727768065281E-4
9	0.0	0.0	0.0	2.7625746643356676E-4	1.1510727768065281E-4
10-11	0.0	0.0	0.0	4.143861996503501E-4	1.2661800544871808E-4
12-13	0.0	0.0	0.0	5.29493477331003E-4	4.949612940268072E-4
14-15	0.0	0.0	0.0	6.215792994755252E-4	5.525149328671335E-4
16-17	0.0	0.0	0.0	9.438796769813531E-4	6.561114827797211E-4
18-19	0.0	0.0	0.0	0.0013237336933275073	7.251758493881128E-4
20-21	0.0	0.0	0.0	0.002164016820396273	7.942402159965044E-4
22-23	0.0	0.0	0.0	0.0034877505137237805	8.287723993007002E-4
24-25	0.0	0.0	0.0	0.004949612940268071	8.517938548368309E-4
26-27	0.0	0.0	0.0	0.006825861566462712	0.0010014333158216795
28-29	0.0	0.0	0.0	0.011844538873339173	0.0010129440435897449
30-31	0.0	0.0	0.0	0.02509338653438231	0.0010129440435897449
32-33	0.0	0.0	0.0	0.05521696110340915	0.001058986954662006
34-35	0.0	0.0	0.0	0.12919640846876473	0.0012201371434149197
36-37	0.0	0.0	0.0	0.23925047665923688	0.0012201371434149197
38-39	0.0	0.0	0.0	0.3451031292143652	0.0012431585989510505
40-41	0.0	0.0	0.0	0.4527859874846159	0.0012431585989510505
42-43	0.0	0.0	0.0	0.5592832407947559	0.0012776907822552464
44-45	0.0	0.0	0.0	0.6846926198278271	0.0013122229655594421
46-47	0.0	0.0	0.0	0.8577103689096164	0.0013582658766317032
48-49	0.0	0.0	0.0	1.068690498170485	0.0014733731543123561
50-51	0.0	0.0	0.0	1.257017515183801	0.0014733731543123561
52-53	0.0	0.0	0.0	1.4258798915413187	0.0015194160653846172
54-55	0.0	0.0	0.0	1.6116860591734286	0.0015884804319930088
56-57	0.0	0.0	0.0	1.820018721047642	0.0016690555263694658
58-59	0.0	0.0	0.0	2.162301721958831	0.0017266091652097923
60-61	0.0	0.0	0.0	2.656365179219729	0.0017726520762820534
62-63	0.0	0.0	0.0	3.0377386116312683	0.0019338022650349674
64-65	0.0	0.0	0.0	3.3378002630891936	0.0019453129928030327
66-67	0.0	1.1510727768065282E-5	0.0	3.668641600598926	0.0019798451761072287
68-69	0.0	2.3021455536130564E-5	0.0	4.015045442051083	0.002002866631643359
70-71	0.0	2.3021455536130564E-5	0.0	4.423054698517925	0.0020258880871794893
72-73	0.0	2.3021455536130564E-5	0.0	4.897089489462389	0.0021179739093240115
74-75	0.0	2.3021455536130564E-5	0.0	5.3529603519888465	0.0021870382759324033
76-77	0.0	3.4532183304195845E-5	0.0	5.768359495682786	0.0021870382759324033
78-79	0.0	4.604291107226113E-5	0.0	6.224345465486925	0.002210059731468534
80-81	0.0	4.604291107226113E-5	0.0	6.714092399834798	0.002210059731468534
82-83	0.0	4.604291107226113E-5	0.0	7.225583098936546	0.002256102642540795
84-85	0.0	4.604291107226113E-5	0.0	8.04499727656181	0.0023021455536130562
86-87	0.0	4.604291107226113E-5	0.0	8.930839864136578	0.0023712099202214476
88	0.0	4.604291107226113E-5	0.0	9.41464726295613	0.0024402742868298395
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCG	575	0.0	26.987629	1
GAGTACT	7940	0.0	21.308634	12-13
GTACCAG	2630	0.0	20.919374	1
CTAACGC	390	0.0	20.496653	3
TATACTC	10255	0.0	20.358507	4
ATACTCT	10530	0.0	19.863018	5
GCTTATA	10785	0.0	19.446136	1
AGTACTT	8510	0.0	18.721634	12-13
TTATACT	11160	0.0	18.707571	3
GTACTTT	8990	0.0	18.689169	14-15
ACTGCGC	760	0.0	17.933208	8
GCATCGT	1020	0.0	17.508827	8
GTACTAG	1245	0.0	17.374292	1
CGACGAG	475	0.0	17.314922	24-25
TAACGCC	490	0.0	17.273289	4
TACCTGG	3120	0.0	16.88018	2
TCTAACG	475	1.8189894E-12	16.829412	2
ACCGTGC	430	8.0035534E-11	16.394434	8
GCTTATG	7420	0.0	16.350628	1
TGATCGC	520	0.0	16.268324	9
>>END_MODULE