FastQCFastQC Report
Mon 16 Jan 2017
ERR1146598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503847
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT657091.875338734824894No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT157590.4497627892998753No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC121120.34567719423821874No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA114730.3274400965567275No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT47430.13536549969219547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC4550.022.7213829
GTACTAG11300.021.6422371
CTAGTAC14700.020.1497693
GAGTACG10450.019.3521581
TACGCAG9850.019.0928444
ATACGGG4750.018.8064523
GTGATCG5750.018.7970498
GTATAAG15500.018.5086771
TAGTACT18400.018.3976154
CGACGAG4650.018.19199224-25
GTACGAC1300.00802623318.0831243
CATGGGG57400.017.774394
GTACATG146600.017.2914731
GTACGCA10900.017.2536243
ACATGGG136050.017.1407573
TAACGCC3602.1900632E-916.9780464
GTACACG4751.8189894E-1216.8318771
TACATGG146000.016.6810652
CCTAGTA16700.016.6104892
AATCGAC3703.1704985E-916.511636
Produced by FastQC (version 0.11.5)