##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146598_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3503847 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.218215863877617 31.0 31.0 34.0 28.0 34.0 2 31.271492448157698 31.0 31.0 34.0 28.0 34.0 3 31.44813001252623 33.0 31.0 34.0 28.0 34.0 4 34.90327203214067 37.0 35.0 37.0 32.0 37.0 5 34.99692452324545 37.0 35.0 37.0 32.0 37.0 6 35.01360932711959 37.0 35.0 37.0 32.0 37.0 7 34.99876678405193 37.0 35.0 37.0 32.0 37.0 8 35.007690975091094 37.0 35.0 37.0 32.0 37.0 9 36.62467310929958 39.0 37.0 39.0 32.0 39.0 10-11 36.626258794975925 39.0 37.0 39.0 32.0 39.0 12-13 36.58988976973024 39.0 37.0 39.0 32.0 39.0 14-15 37.862779111074204 40.0 38.0 41.0 32.0 41.0 16-17 37.910550317979066 40.0 38.0 41.0 32.0 41.0 18-19 37.92433930476987 40.0 38.0 41.0 32.0 41.0 20-21 38.00405839638546 40.0 38.0 41.0 32.0 41.0 22-23 37.97519041213843 40.0 38.0 41.0 32.0 41.0 24-25 37.96209138127321 40.0 38.0 41.0 32.0 41.0 26-27 37.980621585360325 40.0 38.0 41.0 32.0 41.0 28-29 37.969908075324064 40.0 38.0 41.0 32.0 41.0 30-31 37.961556540568125 40.0 38.0 41.0 32.0 41.0 32-33 37.89937403088662 40.0 38.0 41.0 32.0 41.0 34-35 37.8026098171524 40.0 38.0 41.0 31.0 41.0 36-37 37.70109525330301 40.0 38.0 41.0 31.0 41.0 38-39 37.63996901120397 40.0 38.0 41.0 31.0 41.0 40-41 37.64792169863581 40.0 38.0 41.0 31.0 41.0 42-43 37.47276122501924 40.0 37.0 41.0 30.5 41.0 44-45 37.31103841006757 40.0 37.0 41.0 30.0 41.0 46-47 37.16146538361977 40.0 37.0 41.0 30.0 41.0 48-49 37.03588912415411 40.0 36.0 41.0 30.0 41.0 50-51 36.88152878821478 40.0 36.0 41.0 30.0 41.0 52-53 36.83078927247679 39.5 36.0 41.0 30.0 41.0 54-55 36.705383111762586 39.0 35.5 41.0 29.5 41.0 56-57 36.43543410999396 39.0 35.0 41.0 28.5 41.0 58-59 36.24424682356279 39.0 35.0 41.0 28.0 41.0 60-61 35.942220650616306 38.5 35.0 40.0 28.0 41.0 62-63 35.79780038911517 38.0 35.0 40.0 28.0 41.0 64-65 35.54601527977677 37.5 34.0 40.0 28.0 41.0 66-67 35.22504821129461 37.0 34.0 40.0 28.0 41.0 68-69 34.90746599380624 36.5 34.0 39.0 27.5 41.0 70-71 34.51242862488002 36.0 33.5 39.0 26.5 41.0 72-73 34.14794082047532 35.5 33.0 39.0 26.0 40.5 74-75 33.879222751450044 35.0 33.0 37.5 26.0 40.0 76-77 33.61206168534186 35.0 33.0 37.0 26.0 39.0 78-79 33.25191553740788 35.0 33.0 37.0 26.0 39.0 80-81 32.927589589385605 35.0 32.5 36.0 26.0 39.0 82-83 32.63539361165029 35.0 32.0 36.0 25.0 37.0 84-85 32.463759262319385 35.0 32.0 35.5 25.0 37.0 86-87 32.38005169746282 35.0 32.0 35.0 25.0 37.0 88-89 32.18143129537334 35.0 32.0 35.0 25.0 36.0 90-91 31.97901520814122 35.0 32.0 35.0 25.0 36.0 92-93 31.802830574508533 35.0 32.0 35.0 24.0 36.0 94-95 31.685429186833787 35.0 32.0 35.0 24.0 35.5 96-97 31.617862309627103 35.0 32.0 35.0 24.0 35.0 98-99 31.60931755867194 35.0 32.0 35.0 24.0 35.0 100 30.358273349264394 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.278329254903543 1101 2 0.28499747761661 1101 3 0.275021944189799 1101 4 0.2901101318259123 1101 5 0.23867273523487142 1101 6 0.33824529755715105 1101 7 0.3247914984105762 1101 8 0.36083514592527166 1101 9 0.5307758759140881 1101 10-11 0.4916200647564537 1101 12-13 0.5000709560541452 1101 14-15 0.6053878430503801 1101 16-17 0.5810121473225465 1101 18-19 0.5534053106824217 1101 20-21 0.5689460364785432 1101 22-23 0.5547906875503656 1101 24-25 0.5684947479531388 1101 26-27 0.5914482640583856 1101 28-29 0.5356688901215136 1101 30-31 0.6161548842882709 1101 32-33 0.5498936985914042 1101 34-35 0.5598690631373557 1101 36-37 0.5474102531818232 1101 38-39 0.5898591428938929 1101 40-41 0.5096389089976157 1101 42-43 0.49578184244146684 1101 44-45 0.4295613623977488 1101 46-47 0.3996158759527404 1101 48-49 0.5386878862776854 1101 50-51 0.5475757000180508 1101 52-53 0.41212690876956515 1101 54-55 0.4717831948306994 1101 56-57 0.45155121188189185 1101 58-59 0.4677151688246326 1101 60-61 0.4847287446780584 1101 62-63 0.48558108119888743 1101 64-65 0.5380258322736253 1101 66-67 0.4405171012923148 1101 68-69 0.4758337683630671 1101 70-71 0.38155601504401204 1101 72-73 0.4658710376727626 1101 74-75 0.5542672792801895 1101 76-77 0.4860493808529185 1101 78-79 0.42934020502433157 1101 80-81 0.4427745078604346 1101 82-83 0.48247271129768166 1101 84-85 0.29585137919179516 1101 86-87 0.4201221993705744 1101 88-89 0.4065440244424252 1101 90-91 0.4190514045445042 1101 92-93 0.38240101688126416 1101 94-95 0.38811903465981956 1101 96-97 0.38702677840243993 1101 98-99 0.31010460867079104 1101 100 0.26773770521050366 1102 1 0.29566946587258514 1102 2 0.31966611252940424 1102 3 0.28675078273748866 1102 4 0.3379229837995794 1102 5 0.23601148685792595 1102 6 0.31200887602319227 1102 7 0.2922514505889282 1102 8 0.32236772114707435 1102 9 0.41819986831052347 1102 10-11 0.42980834284044533 1102 12-13 0.40672042863154445 1102 14-15 0.5168226608005284 1102 16-17 0.4769531074225313 1102 18-19 0.4334069384144925 1102 20-21 0.4607088703039466 1102 22-23 0.46936983461876025 1102 24-25 0.48059581327571266 1102 26-27 0.4620935195000726 1102 28-29 0.4659396549310628 1102 30-31 0.49179344170605077 1102 32-33 0.4667559737492226 1102 34-35 0.42439947179165927 1102 36-37 0.4722758249648109 1102 38-39 0.4819008427700311 1102 40-41 0.43364206343009926 1102 42-43 0.47185895203834605 1102 44-45 0.38186404198149404 1102 46-47 0.42368703965019705 1102 48-49 0.3937730522128078 1102 50-51 0.3677089682421055 1102 52-53 0.3501526942906068 1102 54-55 0.3207957975929787 1102 56-57 0.3553530888253462 1102 58-59 0.36314489008955064 1102 60-61 0.3195427529027839 1102 62-63 0.3370982397399942 1102 64-65 0.4016783881871575 1102 66-67 0.29459877976471915 1102 68-69 0.3445480097089586 1102 70-71 0.39617800701386585 1102 72-73 0.41068820781379856 1102 74-75 0.3544078816533158 1102 76-77 0.2616248870401705 1102 78-79 0.27337222831399544 1102 80-81 0.2223713541763317 1102 82-83 0.22738342925848798 1102 84-85 0.19847474070173377 1102 86-87 0.2486966615641535 1102 88-89 0.26609319878937043 1102 90-91 0.2274513439255088 1102 92-93 0.19850957725516238 1102 94-95 0.18406598182027523 1102 96-97 0.2339764646430531 1102 98-99 0.2758320552269211 1102 100 0.30135299167263696 1103 1 0.17388003611043246 1103 2 0.17512340515033387 1103 3 0.19151797285551098 1103 4 0.29225454197516143 1103 5 0.23723908171768926 1103 6 0.24136423892709047 1103 7 0.24179318700940655 1103 8 0.31618697413701113 1103 9 0.2838278619491206 1103 10-11 0.3298815437335776 1103 12-13 0.3590457300798917 1103 14-15 0.4269420345647177 1103 16-17 0.47775911133957294 1103 18-19 0.4105757855870493 1103 20-21 0.4211752444557959 1103 22-23 0.394213308152743 1103 24-25 0.4825435959167095 1103 26-27 0.49840646847817993 1103 28-29 0.5270161087159693 1103 30-31 0.4673476641486971 1103 32-33 0.499301585341243 1103 34-35 0.4820599741345859 1103 36-37 0.4984178022118897 1103 38-39 0.49579204776213714 1103 40-41 0.4536475101829467 1103 42-43 0.4868859800604355 1103 44-45 0.3779573774516649 1103 46-47 0.4079571495020744 1103 48-49 0.3597664006050252 1103 50-51 0.475124601325156 1103 52-53 0.407632991772104 1103 54-55 0.2365742537182811 1103 56-57 0.36243982322088186 1103 58-59 0.3798443852632758 1103 60-61 0.39672322270236293 1103 62-63 0.3679530659095249 1103 64-65 0.37799927087153407 1103 66-67 0.41586401821987806 1103 68-69 0.34246315586890574 1103 70-71 0.4444411461771409 1103 72-73 0.41271061710497037 1103 74-75 0.392298277392932 1103 76-77 0.32355255387359705 1103 78-79 0.3291500996984382 1103 80-81 0.3790121514741074 1103 82-83 0.38364847640953315 1103 84-85 0.3623552254446949 1103 86-87 0.3266665315278985 1103 88-89 0.32847070099298037 1103 90-91 0.39435831382002107 1103 92-93 0.3605064384239647 1103 94-95 0.30764663166229056 1103 96-97 0.33815996065706955 1103 98-99 0.3792213684740453 1103 100 0.42614391071923663 1104 1 0.13835469819573376 1104 2 0.0939001284360117 1104 3 0.15107058661863348 1104 4 0.15194858836147773 1104 5 0.124008357988032 1104 6 0.11061627648066974 1104 7 0.18554450550753643 1104 8 0.19778670361221629 1104 9 0.35754207635863366 1104 10-11 0.30997523810674465 1104 12-13 0.35125530199715627 1104 14-15 0.37416281285434394 1104 16-17 0.38305457901517315 1104 18-19 0.3773039591862002 1104 20-21 0.3711905493752923 1104 22-23 0.38869905596351373 1104 24-25 0.4659287097453202 1104 26-27 0.393330871138204 1104 28-29 0.4094953540041928 1104 30-31 0.4390748415492567 1104 32-33 0.3544038973492576 1104 34-35 0.38836697978914003 1104 36-37 0.4153426819441819 1104 38-39 0.40607790160429147 1104 40-41 0.45023087649309446 1104 42-43 0.4320334415809697 1104 44-45 0.4418304252126575 1104 46-47 0.3886246060036953 1104 48-49 0.43366158534642807 1104 50-51 0.33503219270311035 1104 52-53 0.37780106667521096 1104 54-55 0.3975028969440686 1104 56-57 0.3962249159535318 1104 58-59 0.4110009270132977 1104 60-61 0.40365085318369154 1104 62-63 0.4019246442603688 1104 64-65 0.4361141012006442 1104 66-67 0.3447696509817817 1104 68-69 0.3543225725998056 1104 70-71 0.4319128286290095 1104 72-73 0.4490283465443241 1104 74-75 0.357345667490506 1104 76-77 0.32219967137814365 1104 78-79 0.28424920594629555 1104 80-81 0.23595234646705876 1104 82-83 0.28036577800055795 1104 84-85 0.304302786791915 1104 86-87 0.2885754651886572 1104 88-89 0.2452949267501907 1104 90-91 0.27418401698455597 1104 92-93 0.17732361495586346 1104 94-95 0.20617984126936406 1104 96-97 0.24089624931060882 1104 98-99 0.31410522688561215 1104 100 0.23126195852849563 1105 1 0.244493574422755 1105 2 0.2340063907919152 1105 3 0.17268778311574806 1105 4 0.2665137621408675 1105 5 0.22514154589680402 1105 6 0.16378030827938517 1105 7 0.23039873600378513 1105 8 0.24538716654990367 1105 9 0.27533921163013986 1105 10-11 0.330824654482754 1105 12-13 0.3219953007782337 1105 14-15 0.3925219234959414 1105 16-17 0.4289593390455906 1105 18-19 0.43474438738451227 1105 20-21 0.41769985193414527 1105 22-23 0.41536606333674797 1105 24-25 0.4575475663933517 1105 26-27 0.4338011763393297 1105 28-29 0.4097129512229998 1105 30-31 0.464850430410813 1105 32-33 0.4804026066850895 1105 34-35 0.4676325201349272 1105 36-37 0.4668442614682675 1105 38-39 0.48215844400067454 1105 40-41 0.45145892790127107 1105 42-43 0.43232730099062877 1105 44-45 0.34739973952906666 1105 46-47 0.37691014624791563 1105 48-49 0.3351951140464138 1105 50-51 0.38046255587937594 1105 52-53 0.33332576641961253 1105 54-55 0.27319266901772465 1105 56-57 0.42122812198111603 1105 58-59 0.3426860341581133 1105 60-61 0.33044510176065955 1105 62-63 0.3379597912107073 1105 64-65 0.4215361191823277 1105 66-67 0.2915015820007625 1105 68-69 0.26022232995747885 1105 70-71 0.3505487686426534 1105 72-73 0.3338407748848482 1105 74-75 0.2681320774887439 1105 76-77 0.2547976234566107 1105 78-79 0.3630412223662347 1105 80-81 0.262752673799298 1105 82-83 0.3161001297290795 1105 84-85 0.36256273595256516 1105 86-87 0.35243517005635994 1105 88-89 0.3115891492980296 1105 90-91 0.25816181476738365 1105 92-93 0.3125675079494634 1105 94-95 0.3564363552190777 1105 96-97 0.31191396626345025 1105 98-99 0.2674910935732733 1105 100 0.44128596881825644 1106 1 0.09429792624989375 1106 2 0.09569411782732118 1106 3 0.15762259310509918 1106 4 0.14147270022549208 1106 5 0.0701199752096926 1106 6 0.08307716290252642 1106 7 0.16811628829029956 1106 8 0.13428329632877478 1106 9 0.24308438483517847 1106 10-11 0.20178336542765862 1106 12-13 0.25116727103855396 1106 14-15 0.30065754260037636 1106 16-17 0.2832140438496751 1106 18-19 0.2487880024596194 1106 20-21 0.28700576862803473 1106 22-23 0.2642915574111555 1106 24-25 0.24917074978766607 1106 26-27 0.26091313953207873 1106 28-29 0.25878019899511173 1106 30-31 0.1968821724662817 1106 32-33 0.22161325154188205 1106 34-35 0.1997480171180115 1106 36-37 0.1875771110157416 1106 38-39 0.2191152261545568 1106 40-41 0.15269449895284026 1106 42-43 0.21686556857153505 1106 44-45 0.1751440751848321 1106 46-47 0.21941592753800165 1106 48-49 0.15283990651550994 1106 50-51 0.19395871035987255 1106 52-53 0.19812678322264787 1106 54-55 0.1491724082151933 1106 56-57 0.21382237699999962 1106 58-59 0.13550503915259782 1106 60-61 0.18462691929418185 1106 62-63 0.1488084099447704 1106 64-65 0.19575559381134156 1106 66-67 0.1784462561053317 1106 68-69 0.16335545082842629 1106 70-71 0.1197337869192765 1106 72-73 0.13487916149789925 1106 74-75 0.08769705029797592 1106 76-77 0.10233604033977883 1106 78-79 0.14808700763467897 1106 80-81 0.18427624117657615 1106 82-83 0.10874874199945594 1106 84-85 0.2031846003584974 1106 86-87 0.11560923601063422 1106 88-89 0.10732493481985728 1106 90-91 0.14200385813271055 1106 92-93 0.15609731461364973 1106 94-95 0.08668824816389176 1106 96-97 0.08635066126972646 1106 98-99 0.061390055391495224 1106 100 0.18837074352281036 1107 1 0.05234508664225501 1107 2 0.07865006254047557 1107 3 0.07667789826467342 1107 4 0.0910309240670486 1107 5 0.06214639534314159 1107 6 -0.01801625323361833 1107 7 0.06089620377341731 1107 8 0.060737015313428344 1107 9 0.1417231634204228 1107 10-11 0.1247146136009647 1107 12-13 0.20744710704444458 1107 14-15 0.20942060231963922 1107 16-17 0.15793550731469708 1107 18-19 0.19090142634570384 1107 20-21 0.23079735224649767 1107 22-23 0.2635116368004802 1107 24-25 0.2859673122695767 1107 26-27 0.25027265932849474 1107 28-29 0.23588804848796485 1107 30-31 0.2021724987330984 1107 32-33 0.2279962614358837 1107 34-35 0.22276751430919717 1107 36-37 0.19568887869905893 1107 38-39 0.2133663309612075 1107 40-41 0.2205673881366863 1107 42-43 0.267294525823047 1107 44-45 0.16772353958891983 1107 46-47 0.18665363988319683 1107 48-49 0.19120861636943687 1107 50-51 0.23744289659002504 1107 52-53 0.21153812509849956 1107 54-55 0.24698967799312044 1107 56-57 0.28142830329055357 1107 58-59 0.3175324005600757 1107 60-61 0.3463412759571938 1107 62-63 0.3340625099805905 1107 64-65 0.3581205283140534 1107 66-67 0.3202253262731034 1107 68-69 0.31247860096859625 1107 70-71 0.2082544923840146 1107 72-73 0.2254747535519286 1107 74-75 0.355098936215569 1107 76-77 0.29592704469014564 1107 78-79 0.28226735940013725 1107 80-81 0.23616091349967405 1107 82-83 0.2943403821676043 1107 84-85 0.30914480607631134 1107 86-87 0.28152211432404783 1107 88-89 0.22397978977942046 1107 90-91 0.25418993225822106 1107 92-93 0.265410928073063 1107 94-95 0.2138022690447947 1107 96-97 0.21314710288766392 1107 98-99 0.32437075524521575 1107 100 0.3436490932680094 1108 1 0.09774886317745057 1108 2 0.04896660416399001 1108 3 0.12439864670963274 1108 4 0.11737565647048598 1108 5 0.1490654122374977 1108 6 0.09033094839416123 1108 7 0.11345598508675891 1108 8 0.10564128716996635 1108 9 0.1560615385555053 1108 10-11 0.13589957762776805 1108 12-13 0.1490111413014219 1108 14-15 0.17816260915500237 1108 16-17 0.1941483661238621 1108 18-19 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-0.3994149522537427 2215 5 -0.3838593998379949 2215 6 -0.35013534117825174 2215 7 -0.37459144451216275 2215 8 -0.36925554035433095 2215 9 -0.5342553299393344 2215 10-11 -0.5307806125874066 2215 12-13 -0.5138125167140117 2215 14-15 -0.6184385199259168 2215 16-17 -0.6634773218699408 2215 18-19 -0.6700795425216697 2215 20-21 -0.5848163332144338 2215 22-23 -0.5620568394800642 2215 24-25 -0.5286200170495121 2215 26-27 -0.6064589849606534 2215 28-29 -0.6762248417829326 2215 30-31 -0.6933240364467181 2215 32-33 -0.7294506531733447 2215 34-35 -0.7011276345863493 2215 36-37 -0.677187483052748 2215 38-39 -0.7446943403465838 2215 40-41 -0.6614873188006172 2215 42-43 -0.7540244923087087 2215 44-45 -0.7502075059479552 2215 46-47 -0.6555944148842912 2215 48-49 -0.6538528349904027 2215 50-51 -0.6227957316581865 2215 52-53 -0.6027213775559943 2215 54-55 -0.653439350628382 2215 56-57 -0.653532898915671 2215 58-59 -0.6518295863286383 2215 60-61 -0.6850188640959871 2215 62-63 -0.6514847552958756 2215 64-65 -0.6918845473670316 2215 66-67 -0.7059703973320239 2215 68-69 -0.7241199090963875 2215 70-71 -0.6003057773216511 2215 72-73 -0.4631833699420653 2215 74-75 -0.4359723174420367 2215 76-77 -0.5859352761249497 2215 78-79 -0.6267307991071505 2215 80-81 -0.6232435221113093 2215 82-83 -0.5828487501758772 2215 84-85 -0.46273235887975517 2215 86-87 -0.5085226941446841 2215 88-89 -0.4104466404495497 2215 90-91 -0.4497997756853067 2215 92-93 -0.505523629478926 2215 94-95 -0.4989767375820868 2215 96-97 -0.475612267086742 2215 98-99 -0.5152401715385331 2215 100 -0.5181579204327669 2216 1 -0.2927385045209654 2216 2 -0.3124459858772646 2216 3 -0.3431546480090084 2216 4 -0.3267562848565788 2216 5 -0.2753396954129812 2216 6 -0.24995323418134774 2216 7 -0.4047411788461375 2216 8 -0.366198744334838 2216 9 -0.35877146286193806 2216 10-11 -0.4755754450214056 2216 12-13 -0.44819563572274035 2216 14-15 -0.6284469748936274 2216 16-17 -0.6002709465744616 2216 18-19 -0.5811630446959271 2216 20-21 -0.5870007126723493 2216 22-23 -0.6047871608980486 2216 24-25 -0.5559772000248415 2216 26-27 -0.757174383769808 2216 28-29 -0.7805194312927455 2216 30-31 -0.7460803645356435 2216 32-33 -0.7280961570084514 2216 34-35 -0.7602280957977285 2216 36-37 -0.7854822457106536 2216 38-39 -0.7241513100310328 2216 40-41 -0.7429235606772977 2216 42-43 -0.7595357271216443 2216 44-45 -0.7130550727434866 2216 46-47 -0.7561530759068447 2216 48-49 -0.6165658028348133 2216 50-51 -0.6892233290518348 2216 52-53 -0.7920450318249124 2216 54-55 -0.8335801260561695 2216 56-57 -0.8263741040263852 2216 58-59 -0.8633125351181477 2216 60-61 -0.8809255915453917 2216 62-63 -0.9178846145376696 2216 64-65 -0.8667357993741618 2216 66-67 -0.8005345594675504 2216 68-69 -0.8285087178719834 2216 70-71 -0.8544862053850721 2216 72-73 -0.6926966558276462 2216 74-75 -0.6970688703305967 2216 76-77 -0.776343690260731 2216 78-79 -0.8475516847294884 2216 80-81 -0.8351488962490095 2216 82-83 -0.9058343315055168 2216 84-85 -0.7814684898038351 2216 86-87 -0.7849932469091954 2216 88-89 -0.8295677459096602 2216 90-91 -0.8419370218209181 2216 92-93 -0.8084139104440062 2216 94-95 -0.8575546964331444 2216 96-97 -0.9334727812245873 2216 98-99 -0.8769010924451734 2216 100 -0.8284472672595911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1045.0 1 273.0 2 182.0 3 176.0 4 135.0 5 202.0 6 349.0 7 661.0 8 1990.0 9 3130.0 10 4022.0 11 5173.0 12 6935.0 13 8265.0 14 9543.0 15 10299.0 16 10573.0 17 11548.0 18 12656.0 19 13718.0 20 15337.0 21 17243.0 22 19393.0 23 21999.0 24 26148.0 25 30747.0 26 34450.0 27 34883.0 28 38845.0 29 47964.0 30 56467.0 31 66840.0 32 85342.0 33 114623.0 34 166769.0 35 252618.0 36 425290.0 37 777725.0 38 903059.0 39 266629.0 40 601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49614223453294 19.126077854643604 13.348771251419809 23.029008659403647 2 14.31626756941305 22.222257126791067 38.340152575485966 25.121322728309913 3 21.184711425529095 25.04304395910738 30.476322680596112 23.295921934767406 4 11.714378423709652 16.937497607794903 41.10351064309899 30.244613325396447 5 12.415840435951612 39.41899587510665 35.47596801886579 12.689195670075943 6 29.044564735174873 38.27414383791547 17.877228229617096 14.804063197292571 7 25.844216054180652 32.970095120079144 23.321173022906915 17.864515802833292 8 24.635821899162195 32.81577837061312 20.850512084614152 21.69788764561054 9 24.0777896942079 18.321677922218875 22.533334666196357 35.06719771737687 10-11 24.68907399084278 25.398080837120517 28.77778867956972 21.13505649246698 12-13 26.96523861041265 23.7232517915916 27.54539955377288 21.766110044222867 14-15 22.765567558929124 25.648795567788863 27.665429927079543 23.92020694620247 16-17 21.529052922416096 28.586222453840936 27.633185083710504 22.251539540032468 18-19 21.138378699680075 30.521543867306168 26.94404094418062 21.39603648883314 20-21 23.185956999372657 27.26127300215964 25.86239667869514 23.690373319772558 22-23 22.170239012798866 27.502602373719004 26.493671115658213 23.833487497823917 24-25 24.03490822509487 27.48170128755071 26.006193542195433 22.47719694515899 26-27 23.142144716750032 26.09649144728603 28.586912547583903 22.17445128838004 28-29 21.651711437144478 29.720408854029056 26.666785680111854 21.961094028714612 30-31 22.695470106588246 27.064645636565928 26.38384051895894 23.856043737886885 32-33 22.92734490052041 27.59687196631278 26.845756540624286 22.630026592542517 34-35 24.245152694826302 26.91251312591624 26.772441460413148 22.069892718844304 36-37 23.275244214535725 26.26873439782124 28.48031243920881 21.97570894843422 38-39 22.62842353862008 28.019030629114834 27.565703446980542 21.78684238528454 40-41 22.41148304857801 28.423316015924875 26.88244514666238 22.282755788834734 42-43 21.995705043467332 28.140063860161813 25.546507151952476 24.317723944418375 44-45 22.94511435443813 26.6551082257698 26.246668402479976 24.153109017312097 46-47 24.12616401373201 27.759830659308687 26.699337579228487 21.414667747730814 48-49 23.368849256936603 27.18278666806645 27.086915813763152 22.361448261233793 50-51 20.75780212246297 27.90678873923414 28.94585291303044 22.38955622527245 52-53 22.341768363971436 28.826327614230294 25.88698661590765 22.94491740589062 54-55 21.65726133741968 26.86895301553504 27.08403466843733 24.389750978607953 56-57 23.535365524960458 26.031775687318394 26.783923983572095 23.648934804149057 58-59 25.022947633115557 26.331584085184357 26.31971388093971 22.325754400760378 60-61 22.955030431517763 27.164862992106187 28.51266590780112 21.367440668574925 62-63 21.846540443531268 27.43484816713243 27.88388743792785 22.834723951408453 64-65 21.27572469098945 29.542265844066982 26.499242204563345 22.682767260380228 66-67 22.73965573814283 26.944356394259948 25.768499550098873 24.54748831749835 68-69 21.81260963220716 27.224912437077542 26.744573384299812 24.217904546415493 70-71 23.81027477596967 26.956291569334372 26.037886134753457 23.195547519942494 72-73 23.12043849316826 25.734519195360694 28.626743432301506 22.518298879169546 74-75 21.779934086990828 29.180127132027007 26.596517732673625 22.443421048308544 76-77 22.435347576724197 26.722720693446462 26.575205335179263 24.266726394650078 78-79 22.776010266122924 27.029971381882373 27.13031264789944 23.063705704095263 80-81 23.80016933156031 26.48061907869861 26.863179270754365 22.85603231898672 82-83 22.964187900809748 25.883174261778656 28.631408456096423 22.521229381315173 84-85 22.467944556127755 27.53956210523068 27.43531164929564 22.557181689345924 86-87 22.131459455012006 28.036124552685543 26.957798884335038 22.87461710796741 88-89 22.023853396572147 27.665880466760484 25.6855394639785 24.624726672688865 90-91 22.905730332167458 26.225319415959387 26.31563836196885 24.553311889904307 92-93 24.089804013822285 27.33063977853946 26.68766033956959 21.891895868068666 94-95 22.98236753368291 26.83721991848227 27.120443765447405 23.059968782387415 96-97 21.05559345351748 27.28893215731577 28.79336221957889 22.862112169587856 98-99 22.6480910399414 28.091199933260537 26.02060523779076 23.240103789007303 100 22.55258630012514 25.33692367640908 26.060637964902604 26.04985205856318 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2064.0 1 1348.5 2 639.5 3 625.5 4 563.0 5 486.0 6 413.5 7 412.0 8 451.5 9 485.5 10 447.0 11 390.0 12 418.0 13 460.0 14 511.0 15 572.0 16 650.5 17 765.0 18 996.5 19 1328.5 20 1812.5 21 2724.0 22 4030.5 23 5789.5 24 8079.0 25 10927.0 26 14923.0 27 20070.5 28 25565.5 29 31381.0 30 37886.0 31 44166.5 32 50631.5 33 57618.0 34 64354.0 35 70957.0 36 78490.5 37 86179.0 38 89775.5 39 91641.5 40 94294.5 41 96075.0 42 98675.0 43 103696.0 44 109148.0 45 114933.0 46 126497.5 47 175694.5 48 273042.0 49 272035.5 50 183706.5 51 138964.0 52 117821.5 53 107370.5 54 100923.0 55 91692.5 56 82174.5 57 74050.0 58 66460.0 59 59065.0 60 49132.5 61 38728.5 62 30286.5 63 23068.0 64 16754.0 65 11889.5 66 8643.5 67 6325.0 68 4707.0 69 3578.5 70 2887.5 71 2382.5 72 1943.0 73 1697.0 74 1378.5 75 1066.0 76 860.5 77 603.5 78 411.0 79 301.0 80 198.5 81 134.0 82 107.0 83 86.0 84 66.5 85 45.5 86 32.5 87 25.5 88 19.0 89 19.0 90 21.0 91 17.0 92 16.5 93 13.0 94 9.5 95 11.5 96 14.5 97 13.0 98 10.0 99 9.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09632269902196072 2 0.06381557185573457 3 0.07919866364027882 4 0.08256639059867625 5 0.0829944914832183 6 0.09286935188665486 7 0.08887374363092909 8 0.09007242610764683 9 0.07454663402825523 10-11 0.08258066062816098 12-13 0.07109328689294937 14-15 0.08106803750277909 16-17 0.08226671997949682 18-19 0.08235234015640523 20-21 0.07341930169896117 22-23 0.07752907019056483 24-25 0.07936990399409563 26-27 0.07224915928121291 28-29 0.0811108475912333 30-31 0.08539185643665377 32-33 0.08714707006327616 34-35 0.08958724510516584 36-37 0.0894445448103185 38-39 0.09060041719858201 40-41 0.08660480894285623 42-43 0.09217012044190286 44-45 0.08808889200926867 46-47 0.08492094546365753 48-49 0.09431062486461309 50-51 0.09124256852539508 52-53 0.09662236964114015 54-55 0.08800327183236026 56-57 0.09533806698751401 58-59 0.09949064556757188 60-61 0.09836331323827782 62-63 0.0994621055086024 64-65 0.10165969004925159 66-67 0.10370030426556869 68-69 0.10008998680593073 70-71 0.10261578202472882 72-73 0.09977604615726655 74-75 0.10352906391175186 76-77 0.10084629836862168 78-79 0.10820963358274491 80-81 0.10312950308617927 82-83 0.10688252084066457 84-85 0.10338636361690451 86-87 0.10026122715974756 88-89 0.098891304329213 90-91 0.09914816485993823 92-93 0.09328318274171217 94-95 0.09301205218150221 96-97 0.09187044982272342 98-99 0.10471347635898486 100 0.2438748038941198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3503847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.52877836636517 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86098642577814 48.19355793297096 2 13.979899693628923 17.76251898441136 3 4.998344737773235 9.526162051340505 4 2.1983409597582937 5.586316624247486 5 1.0772427636465247 3.4217958389235386 6 0.6158936742842107 2.347618363850875 7 0.38648635261506425 1.718710408583509 8 0.2314149839492742 1.1761208980775515 9 0.16222731314396235 0.9275492719524334 >10 0.4811894873717322 4.479896810302931 >50 0.005986183629002735 0.25843605267773884 >100 0.0015544428632197273 0.13727919579920345 >500 0.0 0.0 >1k 1.8556352506018695E-4 0.3359364901947354 >5k 0.0 0.0 >10k+ 2.474180334135826E-4 4.128101076667159 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 65709 1.875338734824894 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15759 0.4497627892998753 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12112 0.34567719423821874 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11473 0.3274400965567275 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4743 0.13536549969219547 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.8540058969469842E-5 0.0 0.0 0.0 0.0 6 2.8540058969469842E-5 0.0 0.0 0.0 2.8540058969469842E-5 7 2.8540058969469842E-5 0.0 0.0 0.0 2.8540058969469842E-5 8 2.8540058969469842E-5 0.0 0.0 0.0 2.8540058969469842E-5 9 2.8540058969469842E-5 0.0 0.0 2.8540058969469842E-5 2.8540058969469842E-5 10-11 2.8540058969469842E-5 0.0 0.0 2.8540058969469842E-5 2.8540058969469842E-5 12-13 2.8540058969469842E-5 0.0 0.0 5.7080117938939684E-5 1.997804127862889E-4 14-15 2.8540058969469842E-5 0.0 0.0 1.284302653626143E-4 2.854005896946984E-4 16-17 2.8540058969469842E-5 0.0 0.0 2.4259050124049366E-4 3.8529079608784283E-4 18-19 2.8540058969469842E-5 0.0 0.0 3.424807076336381E-4 4.281008845420476E-4 20-21 2.8540058969469842E-5 0.0 0.0 5.42261120419927E-4 5.279910909351921E-4 22-23 2.8540058969469842E-5 0.0 0.0 9.275519165077698E-4 5.708011793893969E-4 24-25 2.8540058969469842E-5 0.0 0.0 0.0013984628895040221 5.708011793893969E-4 26-27 2.8540058969469842E-5 0.0 0.0 0.0026256854251912255 5.993412383588667E-4 28-29 2.8540058969469842E-5 0.0 0.0 0.006307353032252835 5.993412383588667E-4 30-31 2.8540058969469842E-5 0.0 0.0 0.015240391489696897 5.993412383588667E-4 32-33 2.8540058969469842E-5 0.0 0.0 0.03691656627700925 6.564213562978064E-4 34-35 2.8540058969469842E-5 0.0 0.0 0.06812512076012452 6.849614152672762E-4 36-37 2.8540058969469842E-5 0.0 0.0 0.1137606750523068 6.849614152672762E-4 38-39 2.8540058969469842E-5 0.0 0.0 0.18284188778790855 6.849614152672762E-4 40-41 2.8540058969469842E-5 0.0 0.0 0.2631678837574814 6.849614152672762E-4 42-43 2.8540058969469842E-5 0.0 0.0 0.34659047612524174 7.27771503721481E-4 44-45 2.8540058969469842E-5 0.0 0.0 0.446923053432413 7.563115626909509E-4 46-47 2.8540058969469842E-5 0.0 0.0 0.5766947015665924 7.705815921756858E-4 48-49 2.8540058969469842E-5 0.0 0.0 0.7071798511750085 7.991216511451556E-4 50-51 2.8540058969469842E-5 0.0 0.0 0.8497659857864799 8.847418280535651E-4 52-53 2.8540058969469842E-5 0.0 0.0 0.9931227019901268 9.560919754772397E-4 54-55 2.8540058969469842E-5 0.0 0.0 1.1601676671384338 9.989020639314444E-4 56-57 2.8540058969469842E-5 0.0 0.0 1.3753026316502974 0.0010559821818703842 58-59 2.8540058969469842E-5 0.0 0.0 1.5953607563343948 0.001084522240839854 60-61 2.8540058969469842E-5 0.0 0.0 1.8238952785324245 0.0011130622998093238 62-63 2.8540058969469842E-5 0.0 0.0 2.055397966863279 0.0011701424177482636 64-65 2.8540058969469842E-5 0.0 0.0 2.2960192040348795 0.0011701424177482636 66-67 2.8540058969469842E-5 0.0 0.0 2.5775240756802456 0.0011701424177482636 68-69 2.8540058969469842E-5 0.0 0.0 2.905463623268939 0.0011701424177482636 70-71 2.8540058969469842E-5 0.0 0.0 3.251469028185306 0.0011844124472329986 72-73 2.8540058969469842E-5 0.0 0.0 3.586215379838218 0.0012272225356872032 74-75 2.8540058969469842E-5 0.0 0.0 3.9415248439786326 0.001270032624141408 76-77 2.8540058969469842E-5 0.0 0.0 4.310590616542331 0.001284302653626143 78-79 2.8540058969469842E-5 0.0 0.0 4.774637705356427 0.001284302653626143 80-81 2.8540058969469842E-5 0.0 0.0 5.271092031130355 0.001284302653626143 82-83 2.8540058969469842E-5 0.0 0.0 5.737236814278706 0.0013699228305345524 84-85 2.8540058969469842E-5 0.0 0.0 6.2050511908767705 0.0013699228305345524 86-87 2.8540058969469842E-5 0.0 0.0 6.683339769116631 0.0014270029484734922 88 2.8540058969469842E-5 0.0 0.0 7.083043294984056 0.0014840830664124317 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCGC 455 0.0 22.721382 9 GTACTAG 1130 0.0 21.642237 1 CTAGTAC 1470 0.0 20.149769 3 GAGTACG 1045 0.0 19.352158 1 TACGCAG 985 0.0 19.092844 4 ATACGGG 475 0.0 18.806452 3 GTGATCG 575 0.0 18.797049 8 GTATAAG 1550 0.0 18.508677 1 TAGTACT 1840 0.0 18.397615 4 CGACGAG 465 0.0 18.191992 24-25 GTACGAC 130 0.008026233 18.083124 3 CATGGGG 5740 0.0 17.77439 4 GTACATG 14660 0.0 17.291473 1 GTACGCA 1090 0.0 17.253624 3 ACATGGG 13605 0.0 17.140757 3 TAACGCC 360 2.1900632E-9 16.978046 4 GTACACG 475 1.8189894E-12 16.831877 1 TACATGG 14600 0.0 16.681065 2 CCTAGTA 1670 0.0 16.610489 2 AATCGAC 370 3.1704985E-9 16.51163 6 >>END_MODULE