FastQCFastQC Report
Mon 16 Jan 2017
ERR1146598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503847
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT710522.0278282698987713No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT167360.47764642691304726No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140950.4022721311746774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.22963331446835436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.22569478633056753No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG63090.18005923203838525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.12200875209448359No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA35180.10040392745459491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG10600.024.4032461
GTACTAG10450.024.3034671
TACGCAG10500.022.8413664
GAGTACT95900.021.58747912-13
GTACGCA10950.021.4732133
TACCTGG25300.019.1472972
GTACTTT108100.018.86855314-15
AGTACGC12250.018.8126832
CGCAGTG12250.018.8006026
CGACGAG4850.018.41138524-25
CTAGTAC12050.018.3422223
AGTACTT102500.017.5610112-13
GCATCGT11050.017.4379798
CATGGGG53650.016.917214
GTACATG140550.016.8986231
TAGTACT15950.016.805654
GGGCTAT15700.016.7648686
ACATGGG132150.016.5828783
TACATGG139700.016.4964492
ACTTTTT125400.016.43412816-17
Produced by FastQC (version 0.11.5)