FastQCFastQC Report
Mon 16 Jan 2017
ERR1146597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196669
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT176650.5526064788065327No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87200.2727839510440399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38040.1189988703866431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11150.028.6845571
TGATCGC9750.024.0997249
GTATAAG21000.021.5013681
CATGGGG71650.021.26084
CTATTGA20750.019.2507449
TTTGCGT4500.018.8066223
TAGGCAT18150.018.6509025
GTGATCG12900.018.580088
TATTCTC17450.018.3213485
ATTTAGG21100.018.2787081
GGCTATT21650.018.234567
ATGGGGG49600.018.010095
GGGCTAT23050.017.7395526
CTAGTAC17600.017.6312083
ATAAGGT15500.017.593293
CTCGTCG12400.017.4334329
CTAACGC6750.017.4135383
TTTAGGG22300.017.2885972
TGCGAGT12850.017.21775694
CTAGGCA21300.017.2173274
Produced by FastQC (version 0.11.5)