FastQCFastQC Report
Mon 16 Jan 2017
ERR1146597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196669
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT186240.5826064569087385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119710.3744835639848855No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA116550.3645982740158584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114330.3576535449869849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64710.20242946642270437No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC49390.15450457961083866No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40010.1251615353356885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12200.025.8269791
GAGTACT114100.021.78929912-13
AGTACTT122100.020.24619112-13
CTATTGA19600.020.1417129
ATTTAGG19800.019.9513681
TGATCGC9600.019.5822229
GTACTTT126350.019.56517414-15
TAGGCAT18400.019.158325
AGGGCTA20000.019.0357065
GGGCTAT20900.018.8903566
TAGTACT19000.018.8089354
GGCTATT19850.018.7042277
GCTATTG20900.018.6640118
CTAGGCA21700.018.4188884
CTAGTAC18150.018.1356223
TACTTTT138800.017.82715814-15
CATGGGG73650.017.8129434
ACTTTTT142850.017.45333316-17
ACTGCGC10800.017.406428
GTGATCG10800.017.406428
Produced by FastQC (version 0.11.5)