##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3196669
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.346174408423266	33.0	31.0	34.0	30.0	34.0
2	32.44124149231591	34.0	31.0	34.0	30.0	34.0
3	32.5508809326208	34.0	31.0	34.0	31.0	34.0
4	36.027812075632475	37.0	35.0	37.0	35.0	37.0
5	36.07174374325274	37.0	35.0	37.0	35.0	37.0
6	36.14065797866466	37.0	36.0	37.0	35.0	37.0
7	36.11436404582395	37.0	36.0	37.0	35.0	37.0
8	36.12352983683954	37.0	36.0	37.0	35.0	37.0
9	37.911018938776586	39.0	38.0	39.0	35.0	39.0
10-11	37.856365954685955	39.0	38.0	39.0	35.0	39.0
12-13	37.82833881143152	39.0	38.0	39.0	35.0	39.0
14-15	39.1546717849111	40.0	39.0	41.0	36.0	41.0
16-17	39.10062912362838	40.0	39.0	41.0	36.0	41.0
18-19	39.21523201182231	40.0	39.0	41.0	36.0	41.0
20-21	39.26925433943896	41.0	39.0	41.0	36.0	41.0
22-23	39.23367308282465	40.5	39.0	41.0	36.0	41.0
24-25	39.214450416980924	41.0	39.0	41.0	36.0	41.0
26-27	39.10682526092003	40.5	39.0	41.0	35.0	41.0
28-29	39.042292461308946	40.0	39.0	41.0	35.0	41.0
30-31	38.923132172896224	40.0	38.5	41.0	35.0	41.0
32-33	38.803829705233795	40.0	38.0	41.0	35.0	41.0
34-35	38.72998127112942	40.0	38.0	41.0	35.0	41.0
36-37	38.65667011504789	40.0	38.0	41.0	35.0	41.0
38-39	38.55596763380882	40.0	38.0	41.0	35.0	41.0
40-41	38.4636840723891	40.0	38.0	41.0	34.5	41.0
42-43	38.371476058359505	40.0	38.0	41.0	34.0	41.0
44-45	38.23274148809276	40.0	38.0	41.0	34.0	41.0
46-47	37.98804067609127	40.0	37.5	41.0	33.0	41.0
48-49	37.682119262269566	40.0	37.0	41.0	33.0	41.0
50-51	37.53902640529877	40.0	37.0	41.0	33.0	41.0
52-53	37.42420970078541	40.0	36.5	41.0	33.0	41.0
54-55	37.25989099903681	40.0	36.0	41.0	32.0	41.0
56-57	37.08423549638702	39.0	35.5	41.0	32.0	41.0
58-59	36.888851176021035	39.0	35.0	41.0	32.0	41.0
60-61	36.63415949539974	39.0	35.0	41.0	31.0	41.0
62-63	36.36484196518313	38.0	35.0	40.5	31.0	41.0
64-65	36.073553283120646	37.0	35.0	40.0	31.0	41.0
66-67	35.77742988091667	37.0	35.0	40.0	31.0	41.0
68-69	35.47758369727988	36.5	35.0	39.0	30.5	41.0
70-71	35.143346871383926	36.0	35.0	39.0	30.0	41.0
72-73	34.80172814263848	35.0	34.5	38.5	30.0	40.5
74-75	34.50233399203984	35.0	34.0	37.0	30.0	39.5
76-77	34.19655491388067	35.0	34.0	37.0	29.5	39.0
78-79	33.93416819195231	35.0	34.0	36.0	29.0	39.0
80-81	33.71522200140208	35.0	34.0	36.0	29.0	38.0
82-83	33.50200755849292	35.0	34.0	36.0	29.0	37.0
84-85	33.325675101175634	35.0	34.0	35.0	29.0	37.0
86-87	33.17201374305566	35.0	34.0	35.0	29.0	36.5
88-89	32.987232021832725	35.0	34.0	35.0	29.0	36.0
90-91	32.903801895035116	35.0	34.0	35.0	29.0	36.0
92-93	32.82084163233666	35.0	34.0	35.0	29.0	36.0
94-95	32.76785898070773	35.0	34.0	35.0	29.0	35.5
96-97	32.72195291411153	35.0	34.0	35.0	29.0	35.0
98-99	32.663117607734804	35.0	34.0	35.0	28.5	35.0
100	31.88470248249037	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24147232639534622
1101	2	0.32212525590944807
1101	3	0.30855986266339386
1101	4	0.20759715745588636
1101	5	0.19677168652781774
1101	6	0.15563462230240788
1101	7	0.22520277027965818
1101	8	0.1904834850122441
1101	9	0.2603829193270357
1101	10-11	0.30357951987171106
1101	12-13	0.3254594204426624
1101	14-15	0.45934354200666405
1101	16-17	0.5024118248479468
1101	18-19	0.4317775653654792
1101	20-21	0.4186301941481432
1101	22-23	0.4289846421273751
1101	24-25	0.36862624880203754
1101	26-27	0.422134643579021
1101	28-29	0.37205459937224106
1101	30-31	0.45065367536416545
1101	32-33	0.4554555515493419
1101	34-35	0.4136718849883678
1101	36-37	0.47412546248697396
1101	38-39	0.41286069908799305
1101	40-41	0.35988801756241884
1101	42-43	0.43307565239395274
1101	44-45	0.4351601443389441
1101	46-47	0.45600934149416616
1101	48-49	0.5230674246108507
1101	50-51	0.43444980788493837
1101	52-53	0.4452615365170658
1101	54-55	0.4345724314215502
1101	56-57	0.4346605781714814
1101	58-59	0.5092062982371743
1101	60-61	0.43957230194410357
1101	62-63	0.41385328028481894
1101	64-65	0.4405334613407348
1101	66-67	0.36286310945598643
1101	68-69	0.37409820159199825
1101	70-71	0.31090541500467594
1101	72-73	0.3900839277924746
1101	74-75	0.4241644317336366
1101	76-77	0.37942375657134164
1101	78-79	0.31634057007069316
1101	80-81	0.3079417552036716
1101	82-83	0.2820495419996192
1101	84-85	0.3091705314979194
1101	86-87	0.32332625688491845
1101	88-89	0.3558029691889928
1101	90-91	0.350796424632037
1101	92-93	0.3827525737351678
1101	94-95	0.40402024773862877
1101	96-97	0.36475994889614327
1101	98-99	0.3771937765810591
1101	100	0.503834546916071
1102	1	0.31064561377394284
1102	2	0.2966067029672317
1102	3	0.28137005887872135
1102	4	0.2302811034178731
1102	5	0.21298362094086087
1102	6	0.21990448634041826
1102	7	0.2548643167651363
1102	8	0.214020320549821
1102	9	0.24509442983159602
1102	10-11	0.2529336675689464
1102	12-13	0.2560112900324114
1102	14-15	0.45087839968893206
1102	16-17	0.4610860219523616
1102	18-19	0.43679592935311007
1102	20-21	0.38551238701739976
1102	22-23	0.386350712063404
1102	24-25	0.41570343611562777
1102	26-27	0.42691220287605347
1102	28-29	0.4217550446779015
1102	30-31	0.43724089733058236
1102	32-33	0.4055368112434934
1102	34-35	0.4479930526746685
1102	36-37	0.4408334862664205
1102	38-39	0.5090402829544232
1102	40-41	0.46083253685342385
1102	42-43	0.4960981740741559
1102	44-45	0.449660016486213
1102	46-47	0.5065803728541667
1102	48-49	0.5385122714308253
1102	50-51	0.5314705221032838
1102	52-53	0.4579875528310282
1102	54-55	0.49683045945507587
1102	56-57	0.4793836150209714
1102	58-59	0.36893175840902614
1102	60-61	0.41175193861175785
1102	62-63	0.43914659221482566
1102	64-65	0.3995709838705963
1102	66-67	0.39869087003172865
1102	68-69	0.38228803973204606
1102	70-71	0.40996102252883304
1102	72-73	0.40460385979188374
1102	74-75	0.42964947549986476
1102	76-77	0.4845497174555575
1102	78-79	0.43296739756357283
1102	80-81	0.3834614783039285
1102	82-83	0.3638951510017421
1102	84-85	0.40496557722170934
1102	86-87	0.4397200615263941
1102	88-89	0.4455489030915487
1102	90-91	0.41143698297841524
1102	92-93	0.3999616211435253
1102	94-95	0.4107379924763137
1102	96-97	0.3594904908972083
1102	98-99	0.3829621657587907
1102	100	0.34579957314029386
1103	1	0.18764316589861352
1103	2	0.17139356499394154
1103	3	0.18486611280822274
1103	4	0.07023057683130673
1103	5	0.09599953417471596
1103	6	0.10065738839258387
1103	7	0.09953661053581442
1103	8	0.14224452307871616
1103	9	0.19133857713550384
1103	10-11	0.1519550533561116
1103	12-13	0.16850402889362925
1103	14-15	0.3148098799535006
1103	16-17	0.4341088402432902
1103	18-19	0.30362680982025836
1103	20-21	0.26713806220524106
1103	22-23	0.25214362399856327
1103	24-25	0.3009714764197682
1103	26-27	0.26963563296452975
1103	28-29	0.3006736148393685
1103	30-31	0.37658277765640946
1103	32-33	0.31894108062182625
1103	34-35	0.30416979694191326
1103	36-37	0.29480882231750627
1103	38-39	0.23056062425364132
1103	40-41	0.2924447533126582
1103	42-43	0.32046665527872875
1103	44-45	0.32076741349406745
1103	46-47	0.29851160538088806
1103	48-49	0.405138066509636
1103	50-51	0.39617836548125496
1103	52-53	0.2888403677108542
1103	54-55	0.3488463792083465
1103	56-57	0.3255217002505262
1103	58-59	0.3063942510429172
1103	60-61	0.3446527454614383
1103	62-63	0.3660735136389448
1103	64-65	0.38469798377082043
1103	66-67	0.33676400551438945
1103	68-69	0.2929406455264356
1103	70-71	0.31143191349777766
1103	72-73	0.32077139510640507
1103	74-75	0.2523016925602022
1103	76-77	0.32488532820171656
1103	78-79	0.3367319078244151
1103	80-81	0.26319591482574367
1103	82-83	0.30272525709475673
1103	84-85	0.23935893276451736
1103	86-87	0.2535196561954578
1103	88-89	0.3591079257491785
1103	90-91	0.2286558737294584
1103	92-93	0.24321218476922013
1103	94-95	0.26803085540535676
1103	96-97	0.29491978179790124
1103	98-99	0.280215877931397
1103	100	0.3593095178479011
1104	1	0.1982134163809448
1104	2	0.18521368128968163
1104	3	0.17895822956409546
1104	4	0.12240181759698743
1104	5	0.11724635583659193
1104	6	0.11615239677754374
1104	7	0.15105134985908109
1104	8	0.13887259312820532
1104	9	0.19373504355191074
1104	10-11	0.21129417733654066
1104	12-13	0.24999548471890165
1104	14-15	0.3296394131353111
1104	16-17	0.3928431327509472
1104	18-19	0.3047881509010466
1104	20-21	0.29223226570157834
1104	22-23	0.3132185147023563
1104	24-25	0.28164577424661275
1104	26-27	0.2805474111356929
1104	28-29	0.27868023925903884
1104	30-31	0.29533272124240995
1104	32-33	0.2915256643516315
1104	34-35	0.2589409933320965
1104	36-37	0.2766189568525519
1104	38-39	0.3383242077460338
1104	40-41	0.27822024052489525
1104	42-43	0.32652987379621123
1104	44-45	0.36962833363725167
1104	46-47	0.38629015143853707
1104	48-49	0.3921183991748194
1104	50-51	0.3237422738762703
1104	52-53	0.33867874555718913
1104	54-55	0.32928578491237914
1104	56-57	0.3195008840644178
1104	58-59	0.222759798183084
1104	60-61	0.15635228338097562
1104	62-63	0.21652400692289575
1104	64-65	0.27993326318224376
1104	66-67	0.25372077129965476
1104	68-69	0.24006362635155654
1104	70-71	0.2763543749573003
1104	72-73	0.21534429552453105
1104	74-75	0.20841937288398782
1104	76-77	0.2024548412613214
1104	78-79	0.2084039807883329
1104	80-81	0.1792376718859927
1104	82-83	0.16818029813370572
1104	84-85	0.18781055495806953
1104	86-87	0.16923169314678432
1104	88-89	0.28925758918591526
1104	90-91	0.2055691687285588
1104	92-93	0.2406507271030094
1104	94-95	0.24580521160886804
1104	96-97	0.24726989952734613
1104	98-99	0.23260000149094395
1104	100	0.24761789363079956
1105	1	0.14614100427498045
1105	2	0.13882254766851787
1105	3	0.18603115244745538
1105	4	0.17180922619920125
1105	5	0.09638065086520697
1105	6	0.1617334553592329
1105	7	0.1484410084984802
1105	8	0.17883420417390283
1105	9	0.20959871437469246
1105	10-11	0.1964529574208811
1105	12-13	0.20933536317179602
1105	14-15	0.27922478733800915
1105	16-17	0.33717377240554924
1105	18-19	0.24568896509815374
1105	20-21	0.24621615022645926
1105	22-23	0.2880071693505215
1105	24-25	0.24841791196653418
1105	26-27	0.26401256088308145
1105	28-29	0.22805637394655776
1105	30-31	0.2623907150879887
1105	32-33	0.2434565821559005
1105	34-35	0.2541307247348641
1105	36-37	0.23672679361167326
1105	38-39	0.19516763923824243
1105	40-41	0.23688893954795986
1105	42-43	0.23375522340990074
1105	44-45	0.2273835475992172
1105	46-47	0.24200584019050098
1105	48-49	0.3151294588497464
1105	50-51	0.2957525682811948
1105	52-53	0.16985919694104012
1105	54-55	0.12911391279670426
1105	56-57	0.20875224568944617
1105	58-59	0.2317835323064159
1105	60-61	0.1713091002878926
1105	62-63	0.16522672192882482
1105	64-65	0.21501300718419003
1105	66-67	0.2560358474771789
1105	68-69	0.14701163279631402
1105	70-71	0.16778774180480838
1105	72-73	0.17109081014083216
1105	74-75	0.12652786558883378
1105	76-77	0.08225637287664966
1105	78-79	0.11488780706666546
1105	80-81	0.18873185888070765
1105	82-83	0.11554155038068359
1105	84-85	0.09679763856247092
1105	86-87	0.10581509413572121
1105	88-89	0.1935414882276305
1105	90-91	0.1801647136742872
1105	92-93	0.2016533204184583
1105	94-95	0.14338896566148662
1105	96-97	0.149377618001175
1105	98-99	0.13027336561430047
1105	100	0.15809384856051523
1106	1	0.08666907677021385
1106	2	0.1125069796071898
1106	3	0.12503396050966842
1106	4	0.10951776330109197
1106	5	0.08203543991634632
1106	6	0.11609264447270817
1106	7	0.10264119837970753
1106	8	0.11807995694395856
1106	9	0.14538989092120858
1106	10-11	0.12835176009834015
1106	12-13	0.15918300882628245
1106	14-15	0.2709932546255658
1106	16-17	0.30075467796497435
1106	18-19	0.25057090123648607
1106	20-21	0.20305015330701082
1106	22-23	0.17955372610407494
1106	24-25	0.21713077350334942
1106	26-27	0.20820034060173498
1106	28-29	0.17835558506943272
1106	30-31	0.18413436205041478
1106	32-33	0.18186522261207472
1106	34-35	0.19005800194711497
1106	36-37	0.20897186659547629
1106	38-39	0.17369805799995675
1106	40-41	0.14826101782951184
1106	42-43	0.17036032687413183
1106	44-45	0.28723753218881853
1106	46-47	0.29145789996197635
1106	48-49	0.33828521595093264
1106	50-51	0.23264502724304492
1106	52-53	0.27025506965369317
1106	54-55	0.25780366542526423
1106	56-57	0.24133112145109692
1106	58-59	0.2452866114226353
1106	60-61	0.23985894697744214
1106	62-63	0.2263750730886116
1106	64-65	0.2016125652753189
1106	66-67	0.20049468120872405
1106	68-69	0.14047387693231883
1106	70-71	0.1532779862505862
1106	72-73	0.15155603826122643
1106	74-75	0.23882076263515728
1106	76-77	0.1525795438357207
1106	78-79	0.15062525086000278
1106	80-81	0.1909986257465306
1106	82-83	0.1860132034850821
1106	84-85	0.06329612744830371
1106	86-87	0.10743969723520053
1106	88-89	0.15883419309809454
1106	90-91	0.14389701748041972
1106	92-93	0.13635803408120495
1106	94-95	0.1433218475249589
1106	96-97	0.14986460693051384
1106	98-99	0.12438857743321563
1106	100	0.21632915786326024
1107	1	0.10984682560374637
1107	2	0.11365265894521315
1107	3	0.08302587077516677
1107	4	0.07428583376385234
1107	5	0.11294881417465064
1107	6	0.10740707977589636
1107	7	0.09083597485865624
1107	8	0.09525503484611875
1107	9	0.16172067606144935
1107	10-11	0.11281085069933283
1107	12-13	0.09748083049672829
1107	14-15	0.19082478260714453
1107	16-17	0.24501061840576455
1107	18-19	0.19274129859706335
1107	20-21	0.18658064644008476
1107	22-23	0.19213746749635874
1107	24-25	0.12614576222791385
1107	26-27	0.1570016731363566
1107	28-29	0.15382446358967883
1107	30-31	0.21268960434859707
1107	32-33	0.17664325868979347
1107	34-35	0.16808052350416602
1107	36-37	0.13730640899239432
1107	38-39	0.15062697238909806
1107	40-41	0.13363148590198648
1107	42-43	0.1424841752868744
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2216	42-43	-0.5379069061753725
2216	44-45	-0.575843427199537
2216	46-47	-0.6782012428491271
2216	48-49	-0.6011850809707724
2216	50-51	-0.5663279464146669
2216	52-53	-0.5729908431073056
2216	54-55	-0.5867044689386489
2216	56-57	-0.5941135151187424
2216	58-59	-0.61528566889465
2216	60-61	-0.6593026332926186
2216	62-63	-0.6670515290295427
2216	64-65	-0.5883509238625635
2216	66-67	-0.624814813093316
2216	68-69	-0.5445745801881756
2216	70-71	-0.5993535334331099
2216	72-73	-0.5543323587022115
2216	74-75	-0.45847815139893555
2216	76-77	-0.5460515099615009
2216	78-79	-0.561454721158654
2216	80-81	-0.5487639058609162
2216	82-83	-0.5315286893511768
2216	84-85	-0.5072634711855741
2216	86-87	-0.4367279951558558
2216	88-89	-0.4059143865463284
2216	90-91	-0.4870452344121503
2216	92-93	-0.50616772514838
2216	94-95	-0.46213549887853844
2216	96-97	-0.52981827357101
2216	98-99	-0.5310568754936043
2216	100	-0.6486083098959163
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	14.0
8	118.0
9	323.0
10	438.0
11	221.0
12	223.0
13	270.0
14	475.0
15	852.0
16	1487.0
17	2572.0
18	4318.0
19	6365.0
20	8918.0
21	12827.0
22	18815.0
23	22353.0
24	22025.0
25	19877.0
26	19318.0
27	21370.0
28	26136.0
29	33029.0
30	40687.0
31	51565.0
32	65932.0
33	86655.0
34	126244.0
35	203352.0
36	374208.0
37	815929.0
38	932047.0
39	276879.0
40	827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.050439032813905	18.94482589473974	13.27803659670422	23.72669847574214
2	15.127494989139878	21.022024634630323	38.55341856879136	25.297061807438443
3	19.42070178852246	25.988899041843972	30.59392284637001	23.996476323263554
4	12.469726229338875	17.219852207035345	38.4696135536352	31.840808009990578
5	12.948968256376583	38.4685940813601	34.714174121772054	13.868263540491258
6	29.898307587481565	37.42353360016668	17.706167554407912	14.97199125794384
7	25.97470240888325	33.383448015245875	22.531773709374885	18.11007586649599
8	25.991513003436406	33.20865091791263	20.403359136347213	20.39647694230375
9	26.26667087938781	15.590738728807224	20.958736083552022	37.18385430825294
10-11	25.048640513484905	25.201987758184973	27.84011094056887	21.90926078776125
12-13	27.30650248743301	23.246932979298137	27.328056173473076	22.118508359795776
14-15	24.165697558473003	24.34446184972319	26.54261039035183	24.94723020145198
16-17	21.966003244638618	27.206632677002045	27.739673746769284	23.087690331590053
18-19	21.885911759314236	27.24105364776927	29.2326286199434	21.64040597297309
20-21	23.06207946354024	25.755186427337772	28.271968782426416	22.91076532669557
22-23	22.501672835066753	25.530106495229877	28.060052510910577	23.908168158792794
24-25	23.073158340760337	25.939798584088624	28.26839438177678	22.71864869337426
26-27	23.421633830310387	24.884801636708314	28.997203330976706	22.696361202004592
28-29	22.1666209419868	26.082838104289184	29.179592882466093	22.570948071257927
30-31	22.883102058411197	25.710439741173964	28.640321810437097	22.76613638997774
32-33	22.701743255933845	26.007769980060452	28.325265542619142	22.96522122138656
34-35	23.10730450442954	25.71464725184642	28.624586659000347	22.553461584723703
36-37	23.507602290885384	25.07756513970171	29.069268375696193	22.345564193716708
38-39	23.68604180284445	25.07738964731343	28.94057635676866	22.295992193073463
40-41	22.849975083282526	25.4099370935001	29.18313834268323	22.556949480534147
42-43	22.85785609958366	25.779694425666218	28.108008680285636	23.254440794464486
44-45	23.128519110457095	25.87335879121068	27.747662946212802	23.250459152119422
46-47	23.390019423343485	25.887369008176947	27.622894331568265	23.099717236911296
48-49	23.86646637446612	25.741890840542155	27.50111600753661	22.890526777455115
50-51	22.988445159633354	26.21730620217483	27.68572535974166	23.10852327845016
52-53	22.765384209312057	26.642177583651698	27.02883085092655	23.563607356109692
54-55	22.599513648544676	26.17773334945433	27.23106730635527	23.99168569564572
56-57	23.669735705227147	25.832775980414546	26.717590683770364	23.779897630587946
58-59	23.475227923376266	25.625521349256957	27.242832185797965	23.65641854156881
60-61	23.194062772404866	25.833221627622848	27.84123409444938	23.131481505522903
62-63	23.24791180286155	25.77749192359393	27.26074458410771	23.713851689436808
64-65	22.563545652436755	26.43565147794173	27.392056423918916	23.6087464457026
66-67	23.107724890235453	25.554766526466278	27.263793106145094	24.073715477153176
68-69	22.702474925553272	25.887972180276364	27.298369726905094	24.111183167265274
70-71	23.064598501041356	25.80164098665672	26.87423403807696	24.259526474224966
72-73	23.179995273050178	25.299458215218245	27.468677700700233	24.051868811031344
74-75	22.907369296659354	26.537764339111746	26.896522706060427	23.65834365816847
76-77	23.106420253243755	25.413566294648092	27.09324970084701	24.386763751261135
78-79	22.783501977952607	25.597730222937493	27.5953442604181	24.02342353869181
80-81	22.704186693557407	25.923520622203412	27.500051225669957	23.872241458569228
82-83	22.547533370101004	25.483733121778464	27.730804977337204	24.23792853078333
84-85	22.580943356621262	25.82366722151853	27.022829218972745	24.572560202887463
86-87	22.476240111190744	25.966826718687486	27.22476114980938	24.33217202031239
88-89	22.789972937454582	25.277108765405487	26.98565287804274	24.947265419097192
90-91	22.678638295050256	25.09183778489421	27.346434679349034	24.8830892407065
92-93	22.615371356380155	25.860423663212913	27.167988903440506	24.356216076966426
94-95	21.768040972662785	25.806511655261037	27.49064025416465	24.934807117911525
96-97	21.618881850633333	25.455780836042152	27.558493138300417	25.366844175024095
98-99	22.04005673410985	25.80912425633457	26.864411233453854	25.286407776101726
100	21.92844159739132	25.421866361452334	26.855156972292153	25.794535068864192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	913.0
1	535.0
2	565.5
3	1059.5
4	709.5
5	380.0
6	830.0
7	2071.5
8	3734.5
9	4451.5
10	3162.5
11	1781.5
12	1695.5
13	1801.5
14	1986.5
15	2255.5
16	2649.0
17	3066.0
18	3509.5
19	4012.0
20	4597.0
21	5388.5
22	6416.5
23	7751.0
24	9096.5
25	10692.5
26	13025.5
27	15694.0
28	18352.0
29	22329.5
30	26918.5
31	31493.0
32	38243.5
33	43899.5
34	47467.0
35	52398.5
36	59320.5
37	67676.5
38	72351.0
39	72202.0
40	71694.5
41	71753.5
42	73899.5
43	81687.5
44	92465.5
45	104128.5
46	116116.0
47	140377.5
48	198704.0
49	201615.0
50	163904.0
51	149766.5
52	130312.0
53	121724.5
54	115972.5
55	105241.0
56	96417.5
57	88215.5
58	78231.5
59	70434.5
60	59991.5
61	47403.5
62	36809.0
63	28574.0
64	20561.5
65	14622.0
66	11288.0
67	8531.5
68	6409.5
69	4968.0
70	3782.5
71	3009.5
72	2633.0
73	2565.0
74	2092.0
75	1559.5
76	1240.5
77	815.5
78	479.0
79	235.0
80	136.0
81	104.5
82	72.5
83	38.5
84	17.5
85	15.5
86	12.5
87	6.0
88	2.5
89	2.5
90	2.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003034408629733013
2	0.0035036470776298703
3	2.502605055449907E-4
4	0.051897772337392456
5	0.0028154306873811458
6	0.005224188053251682
7	3.128256319312384E-5
8	4.379558847037338E-4
9	6.256512638624768E-5
10-11	7.820640798280961E-5
12-13	0.0
14-15	4.692384478968576E-5
16-17	3.441081951243623E-4
18-19	9.384768957937152E-5
20-21	2.502605055449907E-4
22-23	0.0
24-25	0.0
26-27	2.815430687381146E-4
28-29	0.0
30-31	1.407715343690573E-4
32-33	2.189779423518669E-4
34-35	1.407715343690573E-4
36-37	3.128256319312384E-5
38-39	6.412925454590387E-4
40-41	6.256512638624769E-4
42-43	0.0
44-45	5.005210110899814E-4
46-47	0.0
48-49	1.8769537915874304E-4
50-51	0.0
52-53	2.0333666075530497E-4
54-55	2.971843503346765E-4
56-57	3.441081951243623E-4
58-59	0.0011105309933558964
60-61	7.97705361424658E-4
62-63	3.753907583174861E-4
64-65	3.2846691352780036E-4
66-67	0.0016892584124286872
68-69	0.0027528655609948983
70-71	0.003644418611998928
72-73	0.0032690278536814414
74-75	0.00276850684259146
76-77	0.004504689099809833
78-79	0.0017361822572183733
80-81	9.228356141971534E-4
82-83	6.256512638624769E-4
84-85	6.256512638624769E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	7.820640798280961E-5
94-95	3.128256319312384E-5
96-97	3.753907583174861E-4
98-99	9.384768957937152E-5
100	0.07542225985862158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3196669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.55436547186801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6686742077514	35.91724292046921
2	14.939080586196512	15.403496407089268
3	6.170195178214116	9.54301491751225
4	3.0871321096657764	6.366205481665935
5	1.8163924687458015	4.682148058703483
6	1.125730756942419	3.482180091979926
7	0.7724908629393454	2.7877693390157603
8	0.5450965714393842	2.248168628915858
9	0.3705999545032874	1.7195440948488128
>10	1.469326961976906	11.828095513404538
>50	0.024607538723918396	0.8568353414202842
>100	0.00819630364855148	0.7484542566634685
>500	8.572497412873088E-4	0.2987088677715209
>1k	0.001142999655049732	1.1642427331900258
>5k	9.524997125414433E-5	0.3177618088688961
>10k+	3.809998850165773E-4	2.636131538480752
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	18624	0.5826064569087385	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11971	0.3744835639848855	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	11655	0.3645982740158584	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11433	0.3576535449869849	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6471	0.20242946642270437	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	4939	0.15450457961083866	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	4001	0.1251615353356885	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2513025277249536E-4
2	0.0	0.0	0.0	0.0	1.5641281596561922E-4
3	0.0	0.0	0.0	0.0	1.5641281596561922E-4
4	0.0	0.0	0.0	0.0	1.5641281596561922E-4
5	0.0	0.0	0.0	0.0	1.8769537915874304E-4
6	0.0	0.0	0.0	0.0	3.753907583174861E-4
7	0.0	0.0	0.0	0.0	4.379558847037338E-4
8	0.0	0.0	0.0	0.0	4.379558847037338E-4
9	0.0	0.0	0.0	0.0	4.379558847037338E-4
10-11	0.0	0.0	0.0	0.0	5.005210110899814E-4
12-13	0.0	0.0	0.0	0.0	0.0014546391884802585
14-15	0.0	0.0	0.0	0.0	0.0017205409756218112
16-17	0.0	0.0	0.0	0.0	0.0019395189179736783
18-19	0.0	0.0	0.0	6.256512638624768E-5	0.00203336660755305
20-21	0.0	0.0	0.0	2.3461922394842882E-4	0.0022679858315014785
22-23	0.0	0.0	0.0	5.005210110899814E-4	0.002377474802677412
24-25	0.0	0.0	0.0	8.289879246177819E-4	0.0024400399290636595
26-27	0.0	0.0	0.0	0.002049007889149612	0.0026590178714155266
28-29	0.0	0.0	0.0	0.005849839317114159	0.002721582997801774
30-31	0.0	0.0	0.0	0.015234608275051312	0.002737224279398336
32-33	0.0	0.0	0.0	0.0361470017696546	0.0027528655609948983
34-35	0.0	0.0	0.0	0.0717152761202364	0.0029092783769605174
36-37	0.0	0.0	0.0	0.12652232683458936	0.0029092783769605174
38-39	0.0	0.0	0.0	0.20161611977968316	0.0030500499113295743
40-41	0.0	0.0	0.0	0.2928673566140254	0.0030969737561192603
42-43	0.0	0.0	0.0	0.38042725099157904	0.003128256319312384
44-45	0.0	0.0	0.0	0.49038546061540933	0.0033159516984711274
46-47	0.0	0.0	0.0	0.6254322859201249	0.003550570922419556
48-49	0.0	0.0	0.0	0.771568779876803	0.0037069837383851756
50-51	0.0	0.0	0.0	0.9259013053900795	0.0038164727095611087
52-53	0.0	0.0	0.0	1.0811566665175532	0.0040667332151061
54-55	0.0	0.0	0.0	1.2500825077604218	0.004098015778299224
56-57	0.0	0.0	0.0	1.458267965810661	0.004129298341492347
58-59	0.0	0.0	0.0	1.700066538011912	0.004191863467878595
60-61	0.0	0.0	0.0	1.957240490022583	0.004223146031071719
62-63	0.0	0.0	0.0	2.2165729388935795	0.004410841410230462
64-65	0.0	0.0	0.0	2.4848209182746164	0.004582895507792643
66-67	0.0	0.0	0.0	2.786697653088262	0.004676743197372015
68-69	0.0	0.0	0.0	3.129507621840109	0.0047236670421617
70-71	0.0	0.0	0.0	3.5101382094924434	0.0047705908869513855
72-73	0.0	0.0	0.0	3.8857792283154744	0.005005210110899815
74-75	0.0	0.0	0.0	4.263985417320342	0.005271111898041367
76-77	0.0	0.0	0.0	4.672535692622539	0.005349318306024177
78-79	0.0	0.0	0.0	5.145199581189043	0.005474448558796673
80-81	0.0	0.0	0.0	5.64456313744088	0.005521372403586359
82-83	0.0	0.0	0.0	6.152435550881245	0.0056308613747622915
84-85	0.0	0.0	0.0	6.68533401487611	0.0056308613747622915
86-87	0.0	0.0	0.0	7.232215784618301	0.005818556753921034
88	0.0	0.0	0.0	7.656188363574708	0.006006252133079777
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1220	0.0	25.826979	1
GAGTACT	11410	0.0	21.789299	12-13
AGTACTT	12210	0.0	20.246191	12-13
CTATTGA	1960	0.0	20.141712	9
ATTTAGG	1980	0.0	19.951368	1
TGATCGC	960	0.0	19.582222	9
GTACTTT	12635	0.0	19.565174	14-15
TAGGCAT	1840	0.0	19.15832	5
AGGGCTA	2000	0.0	19.035706	5
GGGCTAT	2090	0.0	18.890356	6
TAGTACT	1900	0.0	18.808935	4
GGCTATT	1985	0.0	18.704227	7
GCTATTG	2090	0.0	18.664011	8
CTAGGCA	2170	0.0	18.418888	4
CTAGTAC	1815	0.0	18.135622	3
TACTTTT	13880	0.0	17.827158	14-15
CATGGGG	7365	0.0	17.812943	4
ACTTTTT	14285	0.0	17.453333	16-17
ACTGCGC	1080	0.0	17.40642	8
GTGATCG	1080	0.0	17.40642	8
>>END_MODULE