FastQCFastQC Report
Mon 16 Jan 2017
ERR1146596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250400
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74600.032.655881
CAACGCA68800.032.390085
GGTATCA48100.032.265571
ATCAACG70800.031.5437363
TATCAAC85350.027.598922
AACGCAG84900.027.5120186
TATTCGC3450.027.2413379
CGCAGAG82250.027.0251648
ACGCAGA83050.026.7091947
TACATGG134550.024.460892
GTACATG140450.024.3435061
ACATGGG131950.023.8023533
GTGATCG15250.023.7281538
TCAACGC97850.023.1108844
GCAGAGT97450.022.4709599
TGATCGC16600.022.3633339
GTACTAG26800.022.2863731
CGTTAAC5550.022.0318431
CATGGGG103050.021.3972134
CTGTGCG26500.021.1017559
Produced by FastQC (version 0.11.5)