##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146596_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4250400
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39568087709392	31.0	31.0	34.0	28.0	34.0
2	31.491721485036702	33.0	31.0	34.0	28.0	34.0
3	31.63541525503482	33.0	31.0	34.0	28.0	34.0
4	35.08707368718238	37.0	35.0	37.0	32.0	37.0
5	35.05263034067382	37.0	35.0	37.0	32.0	37.0
6	35.1446837944664	37.0	35.0	37.0	32.0	37.0
7	34.992087803500844	37.0	35.0	37.0	32.0	37.0
8	34.98402620929795	37.0	35.0	37.0	32.0	37.0
9	36.574870835686056	39.0	37.0	39.0	32.0	39.0
10-11	36.5875018821758	39.0	37.0	39.0	32.0	39.0
12-13	36.54978084415585	39.0	37.0	39.0	32.0	39.0
14-15	37.902858201581026	40.0	38.0	41.0	32.0	41.0
16-17	37.941605848861286	40.0	38.0	41.0	32.0	41.0
18-19	37.976117777150385	40.0	38.0	41.0	32.0	41.0
20-21	38.08757775738754	40.0	38.0	41.0	32.0	41.0
22-23	38.11015927912667	40.0	38.0	41.0	32.0	41.0
24-25	38.14144680500659	40.0	38.0	41.0	32.0	41.0
26-27	38.21340885563711	40.0	38.0	41.0	32.0	41.0
28-29	38.207730919442874	40.0	38.0	41.0	32.0	41.0
30-31	38.18263457556935	40.0	38.0	41.0	32.0	41.0
32-33	38.15536408338039	40.0	38.0	41.0	32.0	41.0
34-35	38.10759775550537	40.0	38.0	41.0	32.0	41.0
36-37	38.03272350837568	40.0	38.0	41.0	32.0	41.0
38-39	37.97263198757764	40.0	38.0	41.0	32.0	41.0
40-41	37.92021127423301	40.0	38.0	41.0	32.0	41.0
42-43	37.767517763034064	40.0	37.5	41.0	31.0	41.0
44-45	37.64404632505176	40.0	37.0	41.0	31.0	41.0
46-47	37.54141716073781	40.0	37.0	41.0	31.0	41.0
48-49	37.43732319311124	40.0	37.0	41.0	30.5	41.0
50-51	37.29815182100508	40.0	36.0	41.0	30.0	41.0
52-53	37.172058865048	40.0	36.0	41.0	30.0	41.0
54-55	37.0653933747412	39.5	35.5	41.0	30.0	41.0
56-57	36.88470673348391	39.0	35.0	41.0	30.0	41.0
58-59	36.696207768680594	39.0	35.0	41.0	30.0	41.0
60-61	36.4945919207604	39.0	35.0	41.0	30.0	41.0
62-63	36.27422536702428	38.0	35.0	40.5	30.0	41.0
64-65	35.99428559664973	37.5	35.0	40.0	29.5	41.0
66-67	35.7742840673819	37.0	35.0	40.0	29.0	41.0
68-69	35.527034984942595	37.0	35.0	40.0	29.0	41.0
70-71	35.27528103237342	36.0	34.0	39.0	29.0	41.0
72-73	34.95220543948805	36.0	34.0	39.0	29.0	41.0
74-75	34.61696158008658	35.0	34.0	39.0	28.5	40.5
76-77	34.342064629211364	35.0	34.0	37.5	28.0	39.5
78-79	34.02488730472426	35.0	34.0	37.0	28.0	39.0
80-81	33.78219461697722	35.0	34.0	37.0	28.0	39.0
82-83	33.492707039337475	35.0	34.0	36.0	27.5	38.0
84-85	33.27600378787879	35.0	34.0	36.0	27.0	37.0
86-87	33.062398244871076	35.0	33.5	35.5	27.0	37.0
88-89	32.88573146056842	35.0	33.0	35.0	27.0	36.5
90-91	32.72926830415961	35.0	33.0	35.0	27.0	36.0
92-93	32.565349143610014	35.0	33.0	35.0	26.0	36.0
94-95	32.44555300677584	35.0	33.0	35.0	26.0	36.0
96-97	32.349754258422735	35.0	33.0	35.0	26.0	35.5
98-99	32.25169466403162	35.0	33.0	35.0	25.0	35.0
100	31.132983013363447	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.297872240995531
1101	2	0.3257006926790531
1101	3	0.3421368730412375
1101	4	0.3589355936328644
1101	5	0.35860675283187504
1101	6	0.37739535277242453
1101	7	0.4016567759177718
1101	8	0.4205916947459869
1101	9	0.6114084044021908
1101	10-11	0.5391619598138462
1101	12-13	0.4994317828104471
1101	14-15	0.6651432712481835
1101	16-17	0.6083945932507859
1101	18-19	0.5902008047091627
1101	20-21	0.5660113686704022
1101	22-23	0.5221403540192711
1101	24-25	0.5708284617581398
1101	26-27	0.5946955476948332
1101	28-29	0.5739017958054333
1101	30-31	0.5732833875120846
1101	32-33	0.6403529230051319
1101	34-35	0.6080659387700322
1101	36-37	0.5963786533511239
1101	38-39	0.6426231804271296
1101	40-41	0.5471386755407366
1101	42-43	0.6669259031584005
1101	44-45	0.5978089567783513
1101	46-47	0.5826856720653524
1101	48-49	0.6077171299150947
1101	50-51	0.6293137480816853
1101	52-53	0.5761973806144027
1101	54-55	0.5481002792409626
1101	56-57	0.5374253054074316
1101	58-59	0.5651024568809007
1101	60-61	0.5769824665210024
1101	62-63	0.4974856891488386
1101	64-65	0.520447290275996
1101	66-67	0.5279159977318884
1101	68-69	0.502593618622079
1101	70-71	0.5286239038945837
1101	72-73	0.4943860597016112
1101	74-75	0.5093674023110566
1101	76-77	0.40600530880841745
1101	78-79	0.45515039377706756
1101	80-81	0.4462862855562335
1101	82-83	0.3967093017123702
1101	84-85	0.34644680510185566
1101	86-87	0.40697218643303756
1101	88-89	0.36011899359065325
1101	90-91	0.3455077993067803
1101	92-93	0.3846247987490514
1101	94-95	0.381664918033529
1101	96-97	0.3825730262812712
1101	98-99	0.40799070736795073
1101	100	0.4099480459119853
1102	1	0.30962610684991887
1102	2	0.3171224129605861
1102	3	0.3393029718700369
1102	4	0.33628764792042176
1102	5	0.24673831992804196
1102	6	0.2898758266479504
1102	7	0.2768734914241193
1102	8	0.3424426081773291
1102	9	0.39457867996215157
1102	10-11	0.39030229259141436
1102	12-13	0.39027616697330103
1102	14-15	0.46032594620817235
1102	16-17	0.4584252657365582
1102	18-19	0.510752943702272
1102	20-21	0.43735600999360713
1102	22-23	0.44768101272067895
1102	24-25	0.5204214640759091
1102	26-27	0.4998325896988476
1102	28-29	0.4726632225422662
1102	30-31	0.5017235218183487
1102	32-33	0.5027242063208703
1102	34-35	0.5029692818286478
1102	36-37	0.5031924072905127
1102	38-39	0.45564525162801317
1102	40-41	0.474102617712191
1102	42-43	0.5105459033380555
1102	44-45	0.4508536299145973
1102	46-47	0.39345105543329595
1102	48-49	0.458637653443013
1102	50-51	0.4833516986026041
1102	52-53	0.42841478840841063
1102	54-55	0.3771378113391535
1102	56-57	0.433697585448094
1102	58-59	0.4414282026216725
1102	60-61	0.4692591326657549
1102	62-63	0.3787806651488168
1102	64-65	0.47057732765745897
1102	66-67	0.4557759784320936
1102	68-69	0.47487862220306454
1102	70-71	0.49258941978783355
1102	72-73	0.4272322014337675
1102	74-75	0.48111986200660795
1102	76-77	0.5056646190112559
1102	78-79	0.5313659311730419
1102	80-81	0.41714954042233643
1102	82-83	0.3655606864266332
1102	84-85	0.3469038874993302
1102	86-87	0.37977879674953385
1102	88-89	0.30568998422373994
1102	90-91	0.3647703013092283
1102	92-93	0.4028244585697607
1102	94-95	0.3949837010263195
1102	96-97	0.40080632147297734
1102	98-99	0.404652421029688
1102	100	0.4249759160963862
1103	1	0.1667373354268804
1103	2	0.22142681762017347
1103	3	0.32380861747483536
1103	4	0.26661373960123314
1103	5	0.22949657026413206
1103	6	0.3624686971797857
1103	7	0.23039890738596824
1103	8	0.2760677791106616
1103	9	0.37274072991098706
1103	10-11	0.3269587587390177
1103	12-13	0.292253767954918
1103	14-15	0.35771312518451737
1103	16-17	0.4100107783782718
1103	18-19	0.37785424099927667
1103	20-21	0.3501747662697454
1103	22-23	0.33993249770203704
1103	24-25	0.3876600293488863
1103	26-27	0.38582379449902504
1103	28-29	0.4205969548016881
1103	30-31	0.4289268377051556
1103	32-33	0.4142938585602636
1103	34-35	0.4140791758506097
1103	36-37	0.4066190945717665
1103	38-39	0.40197670765716254
1103	40-41	0.34275233295102225
1103	42-43	0.33078835419605923
1103	44-45	0.31156312603214786
1103	46-47	0.37895526196975027
1103	48-49	0.3241105775781321
1103	50-51	0.33577647097831687
1103	52-53	0.32520666842508206
1103	54-55	0.20842287575429452
1103	56-57	0.253268425019364
1103	58-59	0.28533852916807945
1103	60-61	0.24681947090710565
1103	62-63	0.28082886288405007
1103	64-65	0.2573365987572771
1103	66-67	0.20872110038484237
1103	68-69	0.2682873035436373
1103	70-71	0.25754591359629586
1103	72-73	0.24305233723579533
1103	74-75	0.27760603421294405
1103	76-77	0.2491726068706157
1103	78-79	0.2724231941846469
1103	80-81	0.17672168138570754
1103	82-83	0.19696832162883737
1103	84-85	0.16949377462743342
1103	86-87	0.15491359635147717
1103	88-89	0.12940744094176893
1103	90-91	0.18465216857075717
1103	92-93	0.18460289003048302
1103	94-95	0.12017867745178279
1103	96-97	0.08336729659252029
1103	98-99	0.07176388702277592
1103	100	0.11181499795671357
1104	1	0.16656623433992124
1104	2	0.15739505118369124
1104	3	0.18054312235212322
1104	4	0.24517076530489845
1104	5	0.1449795354738157
1104	6	0.19367075436460368
1104	7	0.20368279860007021
1104	8	0.2866271258798889
1104	9	0.2156038165891303
1104	10-11	0.2710279567168996
1104	12-13	0.30302805062601834
1104	14-15	0.3596121898319069
1104	16-17	0.341843947916324
1104	18-19	0.35259691126120174
1104	20-21	0.39281211576012254
1104	22-23	0.4186456576884581
1104	24-25	0.40934294476609523
1104	26-27	0.3486232940555922
1104	28-29	0.3375965198440767
1104	30-31	0.4045772034715682
1104	32-33	0.34103280504255196
1104	34-35	0.2808892703545993
1104	36-37	0.3516505999368178
1104	38-39	0.326542319172205
1104	40-41	0.311907638489366
1104	42-43	0.34006952862035433
1104	44-45	0.2951065435686928
1104	46-47	0.24927105185131637
1104	48-49	0.22173000807428167
1104	50-51	0.20352725818862893
1104	52-53	0.17717401608940975
1104	54-55	0.24015080540934974
1104	56-57	0.24321638752388708
1104	58-59	0.1663066634372683
1104	60-61	0.18948740789541318
1104	62-63	0.16472757249366055
1104	64-65	0.17947858093371138
1104	66-67	0.09059244980451098
1104	68-69	0.10852133059723457
1104	70-71	0.14079606169016756
1104	72-73	0.16835914461302082
1104	74-75	0.17685274139492435
1104	76-77	0.15615428198053394
1104	78-79	0.17522536500091945
1104	80-81	0.10808477702455832
1104	82-83	0.09889246414962827
1104	84-85	0.11616363647269878
1104	86-87	0.1244852115408932
1104	88-89	0.1304305963800232
1104	90-91	0.12192194497444575
1104	92-93	0.07715606941078335
1104	94-95	0.08463736108017628
1104	96-97	0.057064651827438695
1104	98-99	0.0986793110168378
1104	100	0.02624847051200163
1105	1	0.19494768072895852
1105	2	0.24815100876801566
1105	3	0.24822213664947768
1105	4	0.2860644775799983
1105	5	0.1757385820993349
1105	6	0.29754736690168215
1105	7	0.26755031842197496
1105	8	0.3241408367843164
1105	9	0.3503236613477583
1105	10-11	0.4051541281180988
1105	12-13	0.44230291749148165
1105	14-15	0.42627468821220305
1105	16-17	0.4773816535044233
1105	18-19	0.45666892270327963
1105	20-21	0.41633579891237105
1105	22-23	0.39914580565610436
1105	24-25	0.44208546776033586
1105	26-27	0.41510489961466135
1105	28-29	0.35134331215486014
1105	30-31	0.44670105401116444
1105	32-33	0.4585287575936263
1105	34-35	0.4207295949867742
1105	36-37	0.4227731321277446
1105	38-39	0.40267664740860454
1105	40-41	0.4361548454114086
1105	42-43	0.43779602281126984
1105	44-45	0.3974238918498685
1105	46-47	0.44543840197160733
1105	48-49	0.44824272988180525
1105	50-51	0.4139029959445679
1105	52-53	0.33514888797470377
1105	54-55	0.3876853637083215
1105	56-57	0.43264900176166776
1105	58-59	0.3689565420277816
1105	60-61	0.3817724101252651
1105	62-63	0.4254400079136289
1105	64-65	0.4513010833745881
1105	66-67	0.3618764103913392
1105	68-69	0.33133586749315924
1105	70-71	0.31401944095811984
1105	72-73	0.3060095397207334
1105	74-75	0.3578602696890485
1105	76-77	0.33562684860035574
1105	78-79	0.3305786605316072
1105	80-81	0.2598480264454608
1105	82-83	0.27420550208356786
1105	84-85	0.2958726746181597
1105	86-87	0.23777771187880603
1105	88-89	0.16879704979515253
1105	90-91	0.18199170777789675
1105	92-93	0.25789074916955457
1105	94-95	0.2263945850260498
1105	96-97	0.28296521130947383
1105	98-99	0.24896855785538463
1105	100	0.3459501471693258
1106	1	0.08611311406624012
1106	2	0.0965922108622621
1106	3	0.10884823972330793
1106	4	0.12928806970450069
1106	5	0.14447850034916598
1106	6	0.1762072278472857
1106	7	0.2051949392176411
1106	8	0.17578806040467754
1106	9	0.24498123779591197
1106	10-11	0.1785590988446515
1106	12-13	0.23115529716078242
1106	14-15	0.26876615431160644
1106	16-17	0.29246478419826616
1106	18-19	0.2621589488713809
1106	20-21	0.26846405628913317
1106	22-23	0.25039029869465423
1106	24-25	0.32919519889102844
1106	26-27	0.2825330965092476
1106	28-29	0.27937632815100955
1106	30-31	0.3152232177786871
1106	32-33	0.3621438762464422
1106	34-35	0.2939992966129168
1106	36-37	0.295576264574386
1106	38-39	0.2947226884499088
1106	40-41	0.2855262423365872
1106	42-43	0.3091990614645823
1106	44-45	0.26653215068375147
1106	46-47	0.28613595419492555
1106	48-49	0.25483803086554957
1106	50-51	0.2769467078693353
1106	52-53	0.28868507079416617
1106	54-55	0.27086864148533607
1106	56-57	0.2783101015197005
1106	58-59	0.25496872343914134
1106	60-61	0.33509351560785916
1106	62-63	0.24175912738051863
1106	64-65	0.2670604663365168
1106	66-67	0.26880916489297135
1106	68-69	0.1971994127236698
1106	70-71	0.2277631997795524
1106	72-73	0.2646616675785367
1106	74-75	0.17935325909754596
1106	76-77	0.185167571645394
1106	78-79	0.17582658750867353
1106	80-81	0.17802013013702833
1106	82-83	0.1325810714930924
1106	84-85	0.15677280550526262
1106	86-87	0.12385935256140357
1106	88-89	0.18614388677443827
1106	90-91	0.2400374821701874
1106	92-93	0.24014159450154438
1106	94-95	0.190028174650557
1106	96-97	0.21747201785188963
1106	98-99	0.22173608826329172
1106	100	0.22297737560322162
1107	1	-0.01466632158726
1107	2	-0.016490180858419023
1107	3	0.03918245645546392
1107	4	0.02162427008214962
1107	5	0.010052262443608129
1107	6	0.038690395994215976
1107	7	0.06447392442098021
1107	8	0.07697037202398604
1107	9	0.09863711948407428
1107	10-11	0.10479284246006415
1107	12-13	0.09180861957488418
1107	14-15	0.17716552912392558
1107	16-17	0.07213235389958328
1107	18-19	0.16199656911148708
1107	20-21	0.13789664275668656
1107	22-23	0.1664416916325422
1107	24-25	0.2247120832485905
1107	26-27	0.15415108659870924
1107	28-29	0.11824630332662167
1107	30-31	0.14605366603262127
1107	32-33	0.1814784649921748
1107	34-35	0.13571341475188348
1107	36-37	0.0903793438990732
1107	38-39	0.14941418931655193
1107	40-41	0.12692815081099695
1107	42-43	0.20471799477810748
1107	44-45	0.11592517949527092
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2216	70-71	-0.5634122520438183
2216	72-73	-0.5232374734736851
2216	74-75	-0.41963825567297164
2216	76-77	-0.4893461341668086
2216	78-79	-0.41285903885995623
2216	80-81	-0.5120465219610892
2216	82-83	-0.47950495664492365
2216	84-85	-0.5064955863215843
2216	86-87	-0.47366824916881
2216	88-89	-0.44945279073078126
2216	90-91	-0.43913207509474006
2216	92-93	-0.4556096932120326
2216	94-95	-0.49274730518220267
2216	96-97	-0.4854166364016166
2216	98-99	-0.4526454384485916
2216	100	-0.5628120139161972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1282.0
1	306.0
2	245.0
3	192.0
4	174.0
5	226.0
6	316.0
7	853.0
8	2174.0
9	3012.0
10	3216.0
11	3590.0
12	4524.0
13	5959.0
14	8521.0
15	10250.0
16	11024.0
17	12331.0
18	13810.0
19	15674.0
20	17383.0
21	19442.0
22	21440.0
23	25974.0
24	32339.0
25	40435.0
26	43376.0
27	39232.0
28	40996.0
29	50421.0
30	58261.0
31	65832.0
32	81835.0
33	109309.0
34	165236.0
35	255099.0
36	458525.0
37	965961.0
38	1211986.0
39	448934.0
40	705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10085766864288	21.41331395269422	13.462557481320777	24.023270897342115
2	14.658124779140868	21.246760242272313	41.0149502650545	23.080164713532316
3	17.117011687605903	27.443339741156592	31.34795987277284	24.091688698464665
4	11.699057760028445	18.42598948147956	41.50358093574095	28.37137182275104
5	12.445850060740238	39.4325762172831	35.96931128647564	12.152262435501026
6	27.978526295562272	40.3534349261132	19.163823538277207	12.504215240047323
7	25.013104447034724	33.9062790670293	24.275534833339222	16.805081652596755
8	24.265744442659987	36.398075047344356	21.477219368399638	17.858961141596016
9	25.787576352543383	15.9564130956605	21.887065926829173	36.36894462496695
10-11	23.62850912419974	27.361826634200582	29.529658505641653	19.480005735958027
12-13	24.133143931345348	25.52162750725237	28.740770684960825	21.604457876441455
14-15	21.914902359176907	26.686859109032714	26.29874697681417	25.09949155497622
16-17	21.339500650297328	29.246296743027006	27.345523626802446	22.06867897987322
18-19	21.06150860459279	29.392962143370795	28.554059673391386	20.99146957864503
20-21	22.486996249999265	28.490211209035316	27.695371153541664	21.327421387423755
22-23	22.718897003924774	28.254726787992716	27.26876523237024	21.757610975712264
24-25	22.134079953887973	28.438894498013678	27.842868740828923	21.584156807269427
26-27	22.052222131707584	28.70292499878747	27.36386821048012	21.880984659024826
28-29	21.60801470233309	29.03354058034658	27.511446463281253	21.84699825403908
30-31	22.15376536894293	28.596718231042466	27.5249452822658	21.7245711177488
32-33	22.29334527041999	28.974530630660116	26.808005917052473	21.92411818186742
34-35	22.343614303718535	28.71920842383677	27.53778538017094	21.39939189227375
36-37	22.299651157697724	28.38271537491717	27.531096510211185	21.786536957173922
38-39	22.182285340470713	28.521354450965276	27.793789423067413	21.502570785496598
40-41	22.080616887233866	28.055375646333935	28.020843279687092	21.843164186745103
42-43	21.87425857509599	28.247813447809634	27.65622775725288	22.221700219841498
44-45	21.809738578428885	28.788303900501255	27.678604753185837	21.723352767884023
46-47	21.91149013805986	28.301906785398163	27.45773799025662	22.328865086285354
48-49	22.223170741328126	28.009244568115637	27.77861312874208	21.98897156181416
50-51	22.152309194342884	28.124091909047966	27.34415216507722	22.37944673153193
52-53	21.712634802302517	28.38255370367361	27.447106441332252	22.45770505269162
54-55	21.844545198087346	28.325553105003415	27.37107403414729	22.45882766276195
56-57	22.233024935939266	28.056517097816226	27.33638284639483	22.374075119849678
58-59	21.75285281423819	28.117418146003985	27.87714211294069	22.252586926817138
60-61	21.958117170843668	28.472830114829	27.38942124384777	22.179631470479556
62-63	22.152092727779063	28.047515509482906	27.244384233639902	22.556007529098128
64-65	21.493971602017517	28.21698158420946	27.50122082286346	22.787825990909568
66-67	22.136697948414437	27.961322957216773	27.31739249318128	22.58458660118751
68-69	21.677368530749657	27.97256099356004	27.576672487632898	22.773397988057404
70-71	21.878621735654068	27.74325169303814	27.555301202407406	22.822825368900382
72-73	22.178431454744736	27.88086859739598	27.638805219346608	22.301894728512682
74-75	22.009156046689547	28.338855921620265	27.382872514467284	22.269115517222904
76-77	21.91627323359009	28.10840783774739	27.382718398631802	22.59260053003072
78-79	21.871794301102735	28.114421672046618	27.36318337654605	22.650600650304597
80-81	21.766689448538955	28.234358580389475	27.583638596291248	22.415313374780325
82-83	22.267231650859458	27.502599271933008	28.143511606077436	22.086657471130096
84-85	22.381307501297385	27.979021892252447	27.272458573512697	22.36721203293747
86-87	21.649555066202236	28.194381626744047	27.676553129290976	22.479510177762744
88-89	22.279908667250005	27.47993132263279	27.505189012892963	22.734970997224245
90-91	22.236559190430995	27.56864356685138	27.59121663789591	22.603580604821712
92-93	22.489531275241625	27.501873325957778	27.51884040769978	22.489754991100817
94-95	21.63645457801628	27.548293539188585	27.880144938901147	22.935106943893988
96-97	21.90220632432149	27.678234705057115	27.53885072385358	22.88070824676781
98-99	21.678605124610744	27.984920660715574	27.381344351854075	22.955129862819607
100	22.225980153875508	27.59046689785022	26.960101379247398	23.223451569026878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2376.0
1	1568.0
2	751.5
3	726.5
4	695.5
5	648.5
6	588.5
7	586.5
8	615.5
9	607.5
10	544.0
11	496.0
12	507.0
13	547.0
14	605.5
15	692.5
16	841.0
17	1069.5
18	1369.5
19	1811.5
20	2499.0
21	3440.5
22	5161.5
23	7434.5
24	10550.0
25	14544.5
26	20194.0
27	27273.0
28	33808.0
29	42306.0
30	52870.5
31	63999.5
32	78538.5
33	92617.0
34	104862.5
35	116461.5
36	129457.5
37	146955.5
38	153448.0
39	150522.0
40	147739.0
41	145706.5
42	144597.5
43	147061.5
44	153895.0
45	166010.5
46	176773.0
47	182703.0
48	184357.0
49	175050.0
50	162074.5
51	156350.0
52	150994.0
53	141356.0
54	131633.0
55	113916.0
56	99267.5
57	83991.0
58	68400.0
59	61863.5
60	50053.5
61	36270.5
62	27164.0
63	20891.0
64	14897.0
65	9049.5
66	6389.0
67	4920.0
68	3559.0
69	2466.0
70	1838.5
71	1442.5
72	1080.0
73	783.0
74	612.5
75	487.5
76	386.0
77	276.0
78	168.5
79	118.5
80	83.0
81	64.0
82	40.5
83	26.0
84	27.0
85	22.0
86	17.5
87	17.0
88	15.0
89	14.5
90	11.0
91	9.0
92	6.0
93	4.5
94	6.0
95	5.5
96	5.0
97	5.0
98	7.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09970826275174101
2	0.06547619047619048
3	0.08253340862036515
4	0.08481554677206851
5	0.08533314511575381
6	0.09185017880670054
7	0.08822699040090345
8	0.0906738189346885
9	0.07436947110860154
10-11	0.08250988142292491
12-13	0.07049924712968192
14-15	0.08073357801618672
16-17	0.08226284584980237
18-19	0.08192170148691888
20-21	0.07216967814793902
22-23	0.07709862601166949
24-25	0.07886316581968757
26-27	0.07251082251082251
28-29	0.08128646715603237
30-31	0.08481554677206851
32-33	0.08683888575192923
34-35	0.08902691511387165
36-37	0.08897986071899115
38-39	0.09029738377564464
40-41	0.08609777903256165
42-43	0.09249717673630718
44-45	0.08830933559194429
46-47	0.08514492753623189
48-49	0.09444993412384717
50-51	0.0906738189346885
52-53	0.09620271033314512
54-55	0.08794466403162056
56-57	0.09502635046113307
58-59	0.09980237154150198
60-61	0.09817899491812536
62-63	0.09899068322981366
64-65	0.10089638622247318
66-67	0.1027432712215321
68-69	0.1001082251082251
70-71	0.10199040090344438
72-73	0.09876717485413139
74-75	0.10242565405608885
76-77	0.09954357236965933
78-79	0.10689582156973461
80-81	0.10196687370600413
82-83	0.10586062488236402
84-85	0.10244918125352907
86-87	0.09896715603237341
88-89	0.09807312252964426
90-91	0.09857895727460944
92-93	0.0927442123094297
94-95	0.09225014116318464
96-97	0.09075616412572934
98-99	0.10488424618859402
100	0.24654150197628458
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4250400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.16442903985021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.28549320124485	31.926029336628307
2	15.269394158402317	14.708833028477434
3	6.823738939758045	9.85984469851317
4	3.6876049863289926	7.104455547641623
5	2.2101550855050034	5.32254288914349
6	1.4179725296085954	4.097750236967405
7	0.9655454241667716	3.255346084692278
8	0.6660986355916861	2.5665808373997447
9	0.4947066819785915	2.1444538391727854
>10	2.1160823443564865	16.335763846625532
>50	0.05018607344926229	1.6197739861525235
>100	0.012821806485697512	0.9316279222175761
>500	1.0006656187156547E-4	0.03142411029678167
>1k	1.0006656187156546E-4	0.09557363607134667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.3527197440240918E-5	0.0
5	0.0	0.0	0.0	4.7054394880481836E-5	0.0
6	0.0	0.0	0.0	4.7054394880481836E-5	0.0
7	0.0	0.0	0.0	7.058159232072275E-5	0.0
8	0.0	0.0	0.0	7.058159232072275E-5	0.0
9	0.0	0.0	0.0	9.410878976096367E-5	0.0
10-11	0.0	0.0	0.0	3.0585356672313194E-4	0.0
12-13	0.0	0.0	0.0	3.0585356672313194E-4	0.0
14-15	0.0	0.0	0.0	3.2938076416337287E-4	0.0
16-17	0.0	0.0	0.0	3.2938076416337287E-4	0.0
18-19	0.0	0.0	0.0	3.2938076416337287E-4	0.0
20-21	0.0	0.0	0.0	3.6467156032373425E-4	1.1763598720120459E-5
22-23	0.0	0.0	0.0	4.940711462450593E-4	2.3527197440240918E-5
24-25	0.0	0.0	0.0	7.411067193675889E-4	2.3527197440240918E-5
26-27	0.0	0.0	0.0	0.0013881046489742142	2.3527197440240918E-5
28-29	0.0	0.0	0.0	0.004764257481648786	2.3527197440240918E-5
30-31	0.0	0.0	0.0	0.01539855072463768	2.3527197440240918E-5
32-33	0.0	0.0	0.0	0.040313852813852816	2.3527197440240918E-5
34-35	0.0	0.0	0.0	0.07948663655185395	2.3527197440240918E-5
36-37	0.0	0.0	0.0	0.14746847355543008	2.3527197440240918E-5
38-39	0.0	0.0	0.0	0.24207133446263882	2.3527197440240918E-5
40-41	0.0	0.0	0.0	0.3451086956521739	2.3527197440240918E-5
42-43	0.0	0.0	0.0	0.45569828722002637	2.3527197440240918E-5
44-45	0.0	0.0	0.0	0.5977084509693205	2.3527197440240918E-5
46-47	0.0	0.0	0.0	0.770115753811406	2.3527197440240918E-5
48-49	0.0	0.0	0.0	0.9589215132693394	2.3527197440240918E-5
50-51	0.0	0.0	0.0	1.1543501788067005	2.3527197440240918E-5
52-53	0.0	0.0	0.0	1.353719649915302	2.3527197440240918E-5
54-55	0.0	0.0	0.0	1.5724284773197816	2.3527197440240918E-5
56-57	0.0	0.0	0.0	1.8363095238095237	2.3527197440240918E-5
58-59	0.0	0.0	0.0	2.1384575569358177	2.3527197440240918E-5
60-61	0.0	0.0	0.0	2.454321946169772	2.3527197440240918E-5
62-63	0.0	0.0	0.0	2.775303500846979	2.3527197440240918E-5
64-65	0.0	0.0	0.0	3.1155655938264637	2.3527197440240918E-5
66-67	0.0	0.0	0.0	3.503293807641634	2.3527197440240918E-5
68-69	0.0	0.0	0.0	3.933265104460757	2.3527197440240918E-5
70-71	0.0	0.0	0.0	4.400491718426501	2.3527197440240918E-5
72-73	0.0	0.0	0.0	4.871753246753247	2.3527197440240918E-5
74-75	0.0	0.0	0.0	5.349861189535103	2.3527197440240918E-5
76-77	0.0	0.0	0.0	5.883952098626011	2.3527197440240918E-5
78-79	0.0	0.0	0.0	6.462944664031621	2.3527197440240918E-5
80-81	0.0	1.1763598720120459E-5	0.0	7.0917325428195	2.3527197440240918E-5
82-83	0.0	2.3527197440240918E-5	0.0	7.738530491247882	2.3527197440240918E-5
84-85	0.0	2.3527197440240918E-5	0.0	8.384987295313383	2.3527197440240918E-5
86-87	0.0	2.3527197440240918E-5	0.0	9.060088462262375	2.3527197440240918E-5
88	0.0	2.3527197440240918E-5	0.0	9.596979107848673	2.3527197440240918E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7460	0.0	32.65588	1
CAACGCA	6880	0.0	32.39008	5
GGTATCA	4810	0.0	32.26557	1
ATCAACG	7080	0.0	31.543736	3
TATCAAC	8535	0.0	27.59892	2
AACGCAG	8490	0.0	27.512018	6
TATTCGC	345	0.0	27.241337	9
CGCAGAG	8225	0.0	27.025164	8
ACGCAGA	8305	0.0	26.709194	7
TACATGG	13455	0.0	24.46089	2
GTACATG	14045	0.0	24.343506	1
ACATGGG	13195	0.0	23.802353	3
GTGATCG	1525	0.0	23.728153	8
TCAACGC	9785	0.0	23.110884	4
GCAGAGT	9745	0.0	22.470959	9
TGATCGC	1660	0.0	22.363333	9
GTACTAG	2680	0.0	22.286373	1
CGTTAAC	555	0.0	22.031843	1
CATGGGG	10305	0.0	21.397213	4
CTGTGCG	2650	0.0	21.101755	9
>>END_MODULE