FastQCFastQC Report
Mon 16 Jan 2017
ERR1146596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250400
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154900.36443628834933184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145770.34295595708639187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79910.18800583474496518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95650.058.313581
GTATCAA173250.042.1840021
GTGGTAT21200.034.828421
TATTCGC3650.034.7655879
ATCAACG210900.033.893913
CAACGCA212700.033.6326875
TATCAAC224850.032.3348772
AACGCAG231850.031.3202786
TCAACGC238300.030.2532984
ACGCAGA245800.029.0438777
CGCAGAG245650.028.9085528
GCAGAGT266700.026.4858959
CTGTGCG26700.023.7629979
GAGTACT193700.023.45043412-13
GTACTAG23700.023.2170751
CAGAGTA267000.023.06770710-11
AGAGTAC259300.022.52021610-11
GCTATTG34350.022.301788
TGGTATC33300.022.031042
CTATTGA35200.021.7632439
Produced by FastQC (version 0.11.5)