FastQCFastQC Report
Mon 16 Jan 2017
ERR1146595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847920
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.040.300511
GTATCAA79350.033.8455241
CAACGCA79000.031.543715
ATCAACG81150.030.7675133
AACGCAG90550.028.133996
TCAACGC89400.028.0860184
TATCAAC98200.026.574672
TGATCGC11350.025.2583589
GTACATG168200.025.1109071
ACGCAGA99150.025.0295317
TACATGG166700.024.6243652
CGCAGAG101500.024.356668
ACATGGG163900.023.9548763
GTGATCG13100.021.8855088
GTACTAG20050.021.3471091
CATGGGG110700.020.3457784
TAACGCC7800.019.893254
GCAGAGT123750.019.2925519
GTATAAG26650.019.0606881
CTAACGC7650.019.0540223
Produced by FastQC (version 0.11.5)