##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146595_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4847920
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.940823487186258	31.0	31.0	34.0	28.0	34.0
2	31.17442263898744	31.0	31.0	34.0	27.0	34.0
3	31.314428868463175	33.0	31.0	34.0	28.0	34.0
4	34.81345566758527	37.0	35.0	37.0	32.0	37.0
5	34.86199999174904	37.0	35.0	37.0	32.0	37.0
6	34.92698641891781	37.0	35.0	37.0	32.0	37.0
7	34.81074481427086	37.0	35.0	37.0	32.0	37.0
8	34.81091932210102	37.0	35.0	37.0	32.0	37.0
9	36.41075512797241	39.0	37.0	39.0	32.0	39.0
10-11	36.39662092196241	39.0	37.0	39.0	32.0	39.0
12-13	36.343843751547055	39.0	37.0	39.0	32.0	39.0
14-15	37.66548591973465	40.0	38.0	41.0	32.0	41.0
16-17	37.69319656677503	40.0	38.0	41.0	32.0	41.0
18-19	37.72373894371194	40.0	38.0	41.0	31.5	41.0
20-21	37.82998388999819	40.0	38.0	41.0	32.0	41.0
22-23	37.845415353388674	40.0	38.0	41.0	32.0	41.0
24-25	37.88038364907011	40.0	38.0	41.0	31.0	41.0
26-27	37.95028599894388	40.0	38.0	41.0	32.0	41.0
28-29	37.93508680011222	40.0	38.0	41.0	31.5	41.0
30-31	37.90335545966105	40.0	38.0	41.0	31.0	41.0
32-33	37.874928113500225	40.0	38.0	41.0	31.0	41.0
34-35	37.82755573524315	40.0	38.0	41.0	31.0	41.0
36-37	37.75493520932689	40.0	38.0	41.0	31.0	41.0
38-39	37.70351047459529	40.0	38.0	41.0	30.0	41.0
40-41	37.6586606214624	40.0	38.0	41.0	30.0	41.0
42-43	37.512739793560954	40.0	37.0	41.0	30.0	41.0
44-45	37.40934184144953	40.0	37.0	41.0	30.0	41.0
46-47	37.3128681991452	40.0	37.0	41.0	30.0	41.0
48-49	37.22107945263123	40.0	36.5	41.0	30.0	41.0
50-51	37.108583371837824	40.0	36.0	41.0	30.0	41.0
52-53	37.00190978811531	40.0	36.0	41.0	30.0	41.0
54-55	36.906153154342476	39.5	35.5	41.0	30.0	41.0
56-57	36.74043651710424	39.0	35.0	41.0	30.0	41.0
58-59	36.57468728856912	39.0	35.0	41.0	29.5	41.0
60-61	36.382991159095035	39.0	35.0	41.0	29.0	41.0
62-63	36.16722130315682	38.5	35.0	40.5	29.0	41.0
64-65	35.89158319444215	38.0	35.0	40.0	29.0	41.0
66-67	35.65880109407746	37.0	35.0	40.0	29.0	41.0
68-69	35.40566438802621	37.0	34.0	40.0	28.5	41.0
70-71	35.11762529084638	36.0	34.0	39.0	28.0	41.0
72-73	34.773334749748344	36.0	34.0	39.0	28.0	41.0
74-75	34.4327187742372	35.5	34.0	38.5	28.0	40.0
76-77	34.14810692008119	35.0	34.0	37.5	28.0	39.5
78-79	33.82421306457202	35.0	34.0	37.0	27.0	39.0
80-81	33.568498861367345	35.0	33.5	36.5	27.0	39.0
82-83	33.28981078483143	35.0	33.0	36.0	27.0	38.0
84-85	33.077451979405595	35.0	33.0	36.0	27.0	37.0
86-87	32.86785724599416	35.0	33.0	35.5	26.0	37.0
88-89	32.68583413092625	35.0	33.0	35.0	26.0	36.5
90-91	32.54532026106041	35.0	33.0	35.0	26.0	36.0
92-93	32.3984652180729	35.0	33.0	35.0	26.0	36.0
94-95	32.28036240284493	35.0	33.0	35.0	25.0	36.0
96-97	32.1931790334824	35.0	33.0	35.0	25.0	35.0
98-99	32.10104539678873	35.0	33.0	35.0	25.0	35.0
100	30.993219360055445	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2419332960017293
1101	2	0.2761943577561503
1101	3	0.3144638848152361
1101	4	0.3213300200288316
1101	5	0.27579602865988306
1101	6	0.3393410281126208
1101	7	0.4267355197440139
1101	8	0.4037495209994191
1101	9	0.4407061235703509
1101	10-11	0.5016501702923364
1101	12-13	0.49327053412891786
1101	14-15	0.6038588825199014
1101	16-17	0.626914333128795
1101	18-19	0.5607410715765866
1101	20-21	0.58532904337941
1101	22-23	0.5728825276618394
1101	24-25	0.5849556790723582
1101	26-27	0.5500122479190495
1101	28-29	0.5521907847112786
1101	30-31	0.5940148836766639
1101	32-33	0.5839219907948419
1101	34-35	0.5311401767131585
1101	36-37	0.5665889865277549
1101	38-39	0.5633404845432253
1101	40-41	0.49813483018177607
1101	42-43	0.5087652431725544
1101	44-45	0.46127212796172046
1101	46-47	0.4404283787371881
1101	48-49	0.5233097458080067
1101	50-51	0.47952500946271925
1101	52-53	0.41782962558894354
1101	54-55	0.42434213590366454
1101	56-57	0.3811831880348464
1101	58-59	0.37926405954205933
1101	60-61	0.41969929831493147
1101	62-63	0.3891500761640998
1101	64-65	0.4981535011997522
1101	66-67	0.44496864675897996
1101	68-69	0.4009995406782494
1101	70-71	0.3801802696122749
1101	72-73	0.3993541369403033
1101	74-75	0.36423338768457825
1101	76-77	0.34014799493191816
1101	78-79	0.329060740026506
1101	80-81	0.37839336204135776
1101	82-83	0.4074925957763966
1101	84-85	0.3114605191266051
1101	86-87	0.34789627097398323
1101	88-89	0.34042734128718877
1101	90-91	0.29255330083300635
1101	92-93	0.30965961003189335
1101	94-95	0.30843608357639596
1101	96-97	0.27422246994677835
1101	98-99	0.2813029395580884
1101	100	0.31598919461943
1102	1	0.14621744525915759
1102	2	0.16890700767565292
1102	3	0.17119885475988994
1102	4	0.2560952930037814
1102	5	0.24302936485109683
1102	6	0.3265116338874421
1102	7	0.31661572703541196
1102	8	0.3042305494543598
1102	9	0.41017936907157804
1102	10-11	0.4265493110237628
1102	12-13	0.41823712077500375
1102	14-15	0.4958533477633509
1102	16-17	0.45245623437958926
1102	18-19	0.4022501501015441
1102	20-21	0.4339411865019258
1102	22-23	0.42989525950716967
1102	24-25	0.4748517952165088
1102	26-27	0.43583491184215717
1102	28-29	0.42772687494399975
1102	30-31	0.45678588193335656
1102	32-33	0.43337282577145686
1102	34-35	0.47383415516475935
1102	36-37	0.4556348125973173
1102	38-39	0.45634890099316294
1102	40-41	0.4286488671246147
1102	42-43	0.4316213823702526
1102	44-45	0.34946937868333805
1102	46-47	0.3660046935991019
1102	48-49	0.3613530283663806
1102	50-51	0.3978279778450684
1102	52-53	0.3453615385570501
1102	54-55	0.30061174808965774
1102	56-57	0.375231538563078
1102	58-59	0.37961024379895747
1102	60-61	0.3284525491508603
1102	62-63	0.34164945695725635
1102	64-65	0.34789305327505105
1102	66-67	0.3766179280027302
1102	68-69	0.3366631875207702
1102	70-71	0.25699878484952166
1102	72-73	0.3435173979693289
1102	74-75	0.4233161518239257
1102	76-77	0.3579606229410288
1102	78-79	0.2986457285473634
1102	80-81	0.2715441008027497
1102	82-83	0.2695798944150951
1102	84-85	0.275334253324921
1102	86-87	0.23019438572730166
1102	88-89	0.2406803587297759
1102	90-91	0.24769995759235997
1102	92-93	0.23375552040884173
1102	94-95	0.2761027931112281
1102	96-97	0.2769156148507079
1102	98-99	0.2689436185898373
1102	100	0.2938201606773667
1103	1	0.10862791357871515
1103	2	0.09636666922622439
1103	3	0.1418580440554429
1103	4	0.145625005715317
1103	5	0.17281291463464754
1103	6	0.18353839500510816
1103	7	0.22901635670876885
1103	8	0.27198340266176046
1103	9	0.3669365512833025
1103	10-11	0.3680585593511836
1103	12-13	0.328834326599484
1103	14-15	0.38179589006952597
1103	16-17	0.3616229010462604
1103	18-19	0.35100949681543625
1103	20-21	0.3172646811890587
1103	22-23	0.29934331145522464
1103	24-25	0.3609527397265424
1103	26-27	0.3234332983041739
1103	28-29	0.3066918493635242
1103	30-31	0.3455963896075076
1103	32-33	0.3456740851181692
1103	34-35	0.38587960971021573
1103	36-37	0.3886424867413538
1103	38-39	0.37296342283496386
1103	40-41	0.32004989034381737
1103	42-43	0.4154397609613625
1103	44-45	0.32345698198912487
1103	46-47	0.34521760190292383
1103	48-49	0.3323890378114811
1103	50-51	0.3184114095648596
1103	52-53	0.25392017688841406
1103	54-55	0.23762473510264215
1103	56-57	0.32144857909829483
1103	58-59	0.3658981225868416
1103	60-61	0.2772187829170889
1103	62-63	0.3254609484960156
1103	64-65	0.314143545207358
1103	66-67	0.3191002184395586
1103	68-69	0.2942035259701967
1103	70-71	0.2960473877617602
1103	72-73	0.3232301095002228
1103	74-75	0.36655109869523983
1103	76-77	0.27195019397605336
1103	78-79	0.2598657206764514
1103	80-81	0.20877845735528666
1103	82-83	0.18850748197905176
1103	84-85	0.23796805891327466
1103	86-87	0.20346868718341682
1103	88-89	0.15774745829686765
1103	90-91	0.20652719096504768
1103	92-93	0.16550876716209473
1103	94-95	0.21543081357561533
1103	96-97	0.1992778738038723
1103	98-99	0.14220808139975816
1103	100	0.1432101646128281
1104	1	0.04194354427750824
1104	2	0.0822366064806026
1104	3	0.09956953337704633
1104	4	0.08648261694744264
1104	5	0.013607683246313229
1104	6	0.09475587204364899
1104	7	0.05863664039434724
1104	8	0.08620291394432655
1104	9	0.09586972331826615
1104	10-11	0.147059874404043
1104	12-13	0.2018417409201234
1104	14-15	0.24346565036685064
1104	16-17	0.23010748277908277
1104	18-19	0.2334222585095631
1104	20-21	0.26653294919932335
1104	22-23	0.29573739565741164
1104	24-25	0.30303974869494965
1104	26-27	0.3498967449620878
1104	28-29	0.32531010157652673
1104	30-31	0.3249914410456114
1104	32-33	0.2811097015717081
1104	34-35	0.23897926826762017
1104	36-37	0.2904227447356007
1104	38-39	0.22206699745069614
1104	40-41	0.2537168888915815
1104	42-43	0.3397389133134894
1104	44-45	0.29891640173071465
1104	46-47	0.2567027836545819
1104	48-49	0.2495537859421404
1104	50-51	0.2909209676017852
1104	52-53	0.29886746045205115
1104	54-55	0.2517454442057456
1104	56-57	0.2799597182472411
1104	58-59	0.24355230525863192
1104	60-61	0.23085251714061883
1104	62-63	0.28017831952661254
1104	64-65	0.31925695639070995
1104	66-67	0.3144748422152759
1104	68-69	0.2312355840209861
1104	70-71	0.2214842201888345
1104	72-73	0.2310907547768437
1104	74-75	0.21163988208428464
1104	76-77	0.1918055866627455
1104	78-79	0.17886819973814028
1104	80-81	0.12192555620181622
1104	82-83	0.12439103397975515
1104	84-85	0.06822984658142417
1104	86-87	0.05576176728939686
1104	88-89	0.06722325245576144
1104	90-91	0.14360904850145317
1104	92-93	0.14004633346902295
1104	94-95	0.18561677091746276
1104	96-97	0.11372686111617725
1104	98-99	0.12125289089030389
1104	100	0.2614738098451035
1105	1	0.20093351350463706
1105	2	0.1813910922185613
1105	3	0.17490142669775466
1105	4	0.22784599328389277
1105	5	0.16714064572708054
1105	6	0.16424017985128359
1105	7	0.22019224855310426
1105	8	0.27563238291501335
1105	9	0.32119668508889276
1105	10-11	0.3252537928164756
1105	12-13	0.37287615820816455
1105	14-15	0.4058762660215578
1105	16-17	0.4142107543694067
1105	18-19	0.40146901946746993
1105	20-21	0.4345873540598575
1105	22-23	0.39473419508139784
1105	24-25	0.39033817158523476
1105	26-27	0.2908928441059686
1105	28-29	0.3489198976076082
1105	30-31	0.39116581572505993
1105	32-33	0.34257259658886596
1105	34-35	0.372373752822007
1105	36-37	0.38165531170605504
1105	38-39	0.3249184197097392
1105	40-41	0.3301067229942376
1105	42-43	0.31198871111993043
1105	44-45	0.25525751211853276
1105	46-47	0.28708248835337
1105	48-49	0.3236696823006753
1105	50-51	0.33204430073785574
1105	52-53	0.2788686686818238
1105	54-55	0.24484398643954108
1105	56-57	0.2951290430194078
1105	58-59	0.2720574044814157
1105	60-61	0.35851493774265464
1105	62-63	0.26406287687068186
1105	64-65	0.34499262037461875
1105	66-67	0.3221475944147443
1105	68-69	0.3065180385520918
1105	70-71	0.34531556610748027
1105	72-73	0.31074428872562976
1105	74-75	0.3271727853864448
1105	76-77	0.26940574644497417
1105	78-79	0.285269105170741
1105	80-81	0.24041664982235744
1105	82-83	0.23274261404840502
1105	84-85	0.2512363414328931
1105	86-87	0.18214371091191595
1105	88-89	0.23897040201981667
1105	90-91	0.25425330620453224
1105	92-93	0.2861288333703911
1105	94-95	0.22265295863198986
1105	96-97	0.23441052190443656
1105	98-99	0.33548423483633627
1105	100	0.33188993404126066
1106	1	0.014571476199371602
1106	2	0.003419454414533618
1106	3	0.010059034298084413
1106	4	0.036736355321998815
1106	5	-0.0016282035376988802
1106	6	0.07530831186735298
1106	7	0.11166457772222316
1106	8	0.15640566204500317
1106	9	0.16264827931548353
1106	10-11	0.14619182759267346
1106	12-13	0.22442324535942504
1106	14-15	0.2597026041665984
1106	16-17	0.2613047972504674
1106	18-19	0.2559514131973799
1106	20-21	0.2503686386807047
1106	22-23	0.27809544546053644
1106	24-25	0.3406122534680307
1106	26-27	0.3122513975402157
1106	28-29	0.22671019982490748
1106	30-31	0.24818430328028995
1106	32-33	0.2963777390148863
1106	34-35	0.31063595228054197
1106	36-37	0.30196805273723726
1106	38-39	0.22395163047652744
1106	40-41	0.22315912490193313
1106	42-43	0.2891611521175079
1106	44-45	0.30032814514931516
1106	46-47	0.2614200219650087
1106	48-49	0.2840140145694221
1106	50-51	0.25918135209680315
1106	52-53	0.25243629484543106
1106	54-55	0.23422054381402546
1106	56-57	0.2952472622424338
1106	58-59	0.20205191122418142
1106	60-61	0.20906157894241062
1106	62-63	0.19694222514839055
1106	64-65	0.3080406889798297
1106	66-67	0.24476996763809922
1106	68-69	0.18796607478275007
1106	70-71	0.19705279368339035
1106	72-73	0.182291059343477
1106	74-75	0.24128971523663267
1106	76-77	0.2663014550931919
1106	78-79	0.2413526070881673
1106	80-81	0.1923369058377986
1106	82-83	0.15952805286990923
1106	84-85	0.21683851421446576
1106	86-87	0.20009603416706767
1106	88-89	0.21918254552404903
1106	90-91	0.22943964021460772
1106	92-93	0.20085360755985704
1106	94-95	0.20682800041239346
1106	96-97	0.18216703117385435
1106	98-99	0.20557142577050058
1106	100	0.3277627188582315
1107	1	-0.057917702746959066
1107	2	-0.014904431780948357
1107	3	0.020559458314039603
1107	4	0.010193721457049776
1107	5	-0.04756303558001207
1107	6	0.09635297534168785
1107	7	0.05854732590249512
1107	8	0.11618746350804798
1107	9	0.17208289565252244
1107	10-11	0.14716896914341504
1107	12-13	0.15567834442590112
1107	14-15	0.21531684125909578
1107	16-17	0.157082146102276
1107	18-19	0.15877463815829174
1107	20-21	0.13528433260313477
1107	22-23	0.17760617762131403
1107	24-25	0.245115599512566
1107	26-27	0.1885566077090104
1107	28-29	0.12498230768330387
1107	30-31	0.19723027624872458
1107	32-33	0.21480194324895763
1107	34-35	0.2345446095848942
1107	36-37	0.21394702905469387
1107	38-39	0.17405317700389134
1107	40-41	0.15779767530568023
1107	42-43	0.26079406452680587
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2216	64-65	-0.5864096667810657
2216	66-67	-0.5520847003282086
2216	68-69	-0.6382018213482397
2216	70-71	-0.6234992288957599
2216	72-73	-0.5658564428200492
2216	74-75	-0.5317724708821032
2216	76-77	-0.5942919382821543
2216	78-79	-0.527177504899953
2216	80-81	-0.5591286123065302
2216	82-83	-0.5166982217116711
2216	84-85	-0.49264845040951144
2216	86-87	-0.5330868126351334
2216	88-89	-0.5535081095272503
2216	90-91	-0.5612188319051086
2216	92-93	-0.5098885319554398
2216	94-95	-0.5622501835730382
2216	96-97	-0.5843180044493508
2216	98-99	-0.6017007170433679
2216	100	-0.6645801118003583
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1504.0
1	383.0
2	248.0
3	234.0
4	190.0
5	290.0
6	398.0
7	831.0
8	2470.0
9	3716.0
10	4072.0
11	5272.0
12	7086.0
13	9544.0
14	12186.0
15	13769.0
16	15218.0
17	16418.0
18	17982.0
19	20400.0
20	22695.0
21	25161.0
22	28156.0
23	32460.0
24	38994.0
25	48075.0
26	51919.0
27	47519.0
28	49718.0
29	61264.0
30	70466.0
31	79076.0
32	99465.0
33	131151.0
34	189364.0
35	288585.0
36	515212.0
37	1075107.0
38	1410792.0
39	449711.0
40	819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34576698431328	20.291500353189225	13.41394293180712	23.94878973069038
2	14.685565987827081	20.899938055879336	40.8893945514356	23.525101404857978
3	17.54487159768921	26.96133696210701	30.59045471220677	24.903336727997015
4	11.819592434681287	18.01269997778598	40.67265123907157	29.495056348461162
5	12.763147517861102	39.11148887043676	35.76202896671722	12.363334644984915
6	29.071591782581685	39.434643172519245	18.839997117738523	12.653767927160544
7	25.83741175183258	33.43994169607842	23.55693148773068	17.165715064358324
8	24.863673076351336	36.1022309468517	21.265616793131585	17.768479183665377
9	25.92690990123434	16.16070037022931	21.048965845391333	36.86342388314502
10-11	23.70784628695543	27.12822433286835	29.56382954345344	19.60009983672278
12-13	24.317461077616876	25.126386922434317	28.72605326219762	21.830098737751186
14-15	22.145178138638062	26.390096573478832	26.108902183741467	25.355823104141635
16-17	21.77262290567264	29.121271251908066	26.967075000030967	22.13903084238833
18-19	21.641607325283335	28.98116624385275	28.04514015514864	21.332086275715273
20-21	22.976075197955904	28.058740971767943	27.457839350984965	21.507344479291188
22-23	22.84124003115096	27.881048336112457	27.09061079311603	22.187100839620552
24-25	22.402828372410074	28.088381683291157	27.51154094356426	21.99724900073451
26-27	22.231112261586794	28.305045874873798	27.223977179296476	22.239864684242935
28-29	22.029813678701174	28.416617335039252	27.36822004090697	22.185348945352597
30-31	22.509313843947346	28.13980876929466	27.23669287546035	22.114184511297648
32-33	22.42875194311944	28.354653323956192	26.900442812907134	22.316151920017234
34-35	22.530608385370574	28.26570049156959	27.347296666619524	21.856394456440313
36-37	22.540877415342866	28.070356000030554	27.179215235383346	22.209551349243235
38-39	22.336068746852746	28.218828539391588	27.405306772424705	22.039795941330965
40-41	22.427474433997972	27.822763581927283	27.621295217477343	22.1284667665974
42-43	22.273199561262018	27.924980966513967	27.366039099296728	22.435780372927287
44-45	22.289255502193196	28.304123739009757	27.31632826232644	22.09029249647061
46-47	22.282368359443225	28.027917579697416	27.097823631981615	22.591890428877743
48-49	22.29330050742986	27.92439581077439	27.405486246599576	22.37681743519618
50-51	22.267903183981158	27.93383126568082	27.139136689696862	22.659128860641157
52-53	22.098973651617612	28.087415063288745	27.21866783971097	22.594943445382672
54-55	22.208495114036104	27.957450002007793	27.283036993306347	22.55101789064975
56-57	22.34031823362797	27.944780123834263	27.182256995370025	22.53264464716775
58-59	22.165661818155165	27.959634957062125	27.405630462215175	22.469072762567542
60-61	22.285220394724618	28.16281223842051	27.144038186528093	22.407929180326786
62-63	22.261861413810756	27.882265406641427	27.198275991117725	22.65759718843009
64-65	22.030217133380166	27.814641873841374	27.363910160078003	22.791230832700464
66-67	22.276381533927715	27.832230760240634	27.09021882279966	22.80116888303199
68-69	22.119500905722848	27.81372358532037	27.316840704896645	22.749934804060132
70-71	22.23848069320623	27.63787038280732	27.25159719431078	22.87205172967567
72-73	22.382050511456605	27.660985123205183	27.318486099598406	22.638478265739806
74-75	22.231545165893678	27.966466724111694	27.231193103458246	22.570795006536386
76-77	22.19949081463464	27.843158105566147	27.242284272449275	22.71506680734994
78-79	22.232626187223875	27.771193991787648	27.18601484087922	22.810164980109256
80-81	22.102724781180612	27.91767612975723	27.25633971729045	22.723259371771707
82-83	22.458730820171645	27.61452349692879	27.557933375430903	22.36881230746866
84-85	22.49618177251035	27.67872933684102	27.13727561557529	22.68781327507334
86-87	22.051203641654414	27.96147942004184	27.369076682400134	22.61824025590361
88-89	22.441222898214672	27.48645749558391	27.277633987427436	22.794685618773983
90-91	22.467573468409295	27.49010861220763	27.30484511786041	22.73747280152266
92-93	22.629172781128005	27.58119221801773	27.121759808645535	22.66787519220873
94-95	22.175743016066555	27.47344341544637	27.398588734798984	22.952224833688096
96-97	22.24885020782561	27.441125024878787	27.333227486660427	22.97679728063518
98-99	22.163843563089475	27.644289465503334	27.204916320767175	22.98695065064002
100	22.62403269572209	27.484179656077863	26.916607628588245	22.975180019611805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3210.0
1	2167.5
2	1103.0
3	1042.5
4	964.0
5	864.0
6	763.5
7	738.5
8	741.5
9	761.0
10	715.5
11	628.5
12	633.5
13	667.0
14	717.5
15	842.0
16	1072.5
17	1342.0
18	1652.0
19	2129.0
20	2936.0
21	4218.5
22	6105.0
23	8792.0
24	12297.5
25	16921.5
26	24189.5
27	31930.0
28	38235.5
29	47230.5
30	59982.5
31	72579.0
32	85137.0
33	98853.5
34	113402.0
35	125076.0
36	138702.0
37	154543.0
38	160085.5
39	160343.5
40	160031.0
41	160525.0
42	163326.0
43	168215.5
44	174695.5
45	182256.0
46	188009.5
47	192174.5
48	195374.0
49	192547.0
50	185103.0
51	178754.0
52	172029.5
53	165114.0
54	156612.0
55	140390.5
56	125180.0
57	109478.5
58	93639.5
59	82647.5
60	68437.0
61	52742.0
62	40536.0
63	30721.5
64	22007.0
65	15229.0
66	11447.0
67	8804.5
68	6797.5
69	5290.0
70	4087.0
71	3110.0
72	2491.0
73	2106.5
74	1593.5
75	1122.0
76	794.0
77	541.0
78	336.5
79	214.0
80	140.0
81	100.0
82	80.0
83	58.0
84	47.5
85	39.5
86	27.5
87	27.5
88	22.5
89	17.0
90	16.5
91	10.5
92	13.0
93	16.5
94	12.5
95	10.5
96	9.0
97	8.5
98	8.0
99	10.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10121866697470257
2	0.06664713939173914
3	0.08339659070281688
4	0.08523242957804585
5	0.08568623244607997
6	0.09284394131916368
7	0.08941979240581528
8	0.09109061205630456
9	0.07477433621016848
10-11	0.08273651380385814
12-13	0.0715977161339296
14-15	0.08127196818429347
16-17	0.082550867176026
18-19	0.08218988762190796
20-21	0.07287661512566214
22-23	0.07771374115084408
24-25	0.07966303074308158
26-27	0.07316539876895659
28-29	0.08256118087757224
30-31	0.08641850525586231
32-33	0.08718171917028333
34-35	0.0898013993630258
36-37	0.08979108566147956
38-39	0.09098747504084226
40-41	0.08728485618574564
42-43	0.09246233436195317
44-45	0.08882159771613393
46-47	0.08585125167081965
48-49	0.09469009389593887
50-51	0.09125563128104423
52-53	0.09656718757735276
54-55	0.0887081469991254
56-57	0.09471072129903134
58-59	0.09939314180101982
60-61	0.09789765507681644
62-63	0.09879494711133847
64-65	0.1011980395716101
66-67	0.10326077988085612
68-69	0.09956847472730573
70-71	0.10261101668344362
72-73	0.09984694466905394
74-75	0.10373521015198271
76-77	0.10086800112213073
78-79	0.1075719071271803
80-81	0.10311638805920889
82-83	0.10730375088697833
84-85	0.10378677865971386
86-87	0.09997070908760872
88-89	0.09973349395204542
90-91	0.09938282809947359
92-93	0.09390625257842539
94-95	0.09355558672585357
96-97	0.09150316011815376
98-99	0.10543697090711068
100	0.24872522648888595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4847920.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.69774499828712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.61127912279034	37.37968715340184
2	15.601525231562022	16.7553344693752
3	6.195707205759308	9.980865168567403
4	3.0462818952241877	6.54313873610589
5	1.6944182357783526	4.5493219172636765
6	1.0684428462690116	3.442378290251849
7	0.6945765574543146	2.6108036404778936
8	0.45469794081913806	1.953300326188186
9	0.32674312041132986	1.5790831883812997
>10	1.2330641489686167	10.452390337783068
>50	0.04616860877069292	1.7059354481099607
>100	0.025680763506261146	2.4617244861428516
>500	0.0012728904177139169	0.4341449600437604
>1k	1.4143226862872572E-4	0.15189187790709116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.188220927738081E-5
2	0.0	0.0	0.0	0.0	6.188220927738081E-5
3	0.0	0.0	0.0	0.0	6.188220927738081E-5
4	0.0	0.0	0.0	0.0	6.188220927738081E-5
5	0.0	0.0	0.0	0.0	8.250961236984108E-5
6	0.0	0.0	0.0	0.0	8.250961236984108E-5
7	0.0	0.0	0.0	0.0	8.250961236984108E-5
8	0.0	0.0	0.0	2.062740309246027E-5	8.250961236984108E-5
9	0.0	0.0	0.0	4.125480618492054E-5	8.250961236984108E-5
10-11	0.0	0.0	0.0	5.156850773115068E-5	8.250961236984108E-5
12-13	0.0	0.0	0.0	6.188220927738081E-5	2.578425386557534E-4
14-15	0.0	0.0	0.0	6.188220927738081E-5	3.094110463869041E-4
16-17	0.0	0.0	0.0	8.250961236984108E-5	3.506658525718246E-4
18-19	0.0	0.0	0.0	1.1345071700853148E-4	3.506658525718246E-4
20-21	0.0	0.0	0.0	1.753329262859123E-4	3.9192065875674515E-4
22-23	0.0	0.0	0.0	2.4752883710952325E-4	3.9192065875674515E-4
24-25	0.0	0.0	0.0	7.425865113285697E-4	3.9192065875674515E-4
26-27	0.0	0.0	0.0	0.0016398785458505917	4.228617633954356E-4
28-29	0.0	0.0	0.0	0.0043833231571478075	4.331754649416657E-4
30-31	0.0	0.0	0.0	0.013098400963712273	4.331754649416657E-4
32-33	0.0	0.0	0.0	0.03154961302991799	4.331754649416657E-4
34-35	0.0	0.0	0.0	0.06249071766860839	4.950576742190465E-4
36-37	0.0	0.0	0.0	0.11364667733790987	4.950576742190465E-4
38-39	0.0	0.0	0.0	0.19041155794650078	5.569398834964274E-4
40-41	0.0	0.0	0.0	0.2742619515173518	5.569398834964274E-4
42-43	0.0	0.0	0.0	0.36051543754847437	5.878809881351177E-4
44-45	0.0	0.0	0.0	0.47053169194211125	6.188220927738082E-4
46-47	0.0	0.0	0.0	0.6084980775260318	6.394494958662685E-4
48-49	0.0	0.0	0.0	0.762223799072592	6.600768989587286E-4
50-51	0.0	0.0	0.0	0.9190539447845674	6.600768989587286E-4
52-53	0.0	0.0	0.0	1.0786791036155714	6.807043020511889E-4
54-55	0.0	0.0	0.0	1.2545174012772486	6.807043020511889E-4
56-57	0.0	0.0	0.0	1.4657523226455882	6.807043020511889E-4
58-59	0.0	0.0	0.0	1.7059171768511032	6.807043020511889E-4
60-61	0.0	0.0	0.0	1.959737371903827	6.807043020511889E-4
62-63	0.0	0.0	0.0	2.221653822670341	7.013317051436492E-4
64-65	0.0	0.0	0.0	2.4946678163005993	7.013317051436492E-4
66-67	0.0	0.0	0.0	2.8050483506328487	7.013317051436492E-4
68-69	0.0	0.0	0.0	3.1567146322546575	7.013317051436492E-4
70-71	0.0	0.0	0.0	3.5368570438456084	7.425865113285697E-4
72-73	0.0	0.0	0.0	3.9234145777983134	8.250961236984109E-4
74-75	0.0	0.0	0.0	4.309116074522682	9.179194376144821E-4
76-77	0.0	0.0	0.0	4.7374028449314345	9.488605422531725E-4
78-79	0.0	0.0	0.0	5.210492334857011	9.798016468918628E-4
80-81	0.0	0.0	0.0	5.728972425287546	0.0010313701546230136
82-83	0.0	0.0	0.0	6.261427581313223	0.0011138797669928548
84-85	0.0	0.0	0.0	6.78009331837159	0.0011138797669928548
86-87	0.0	0.0	0.0	7.328245928150629	0.001206703080908926
88	0.0	0.0	0.0	7.763350055281441	0.0012995263948249972
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4680	0.0	40.30051	1
GTATCAA	7935	0.0	33.845524	1
CAACGCA	7900	0.0	31.54371	5
ATCAACG	8115	0.0	30.767513	3
AACGCAG	9055	0.0	28.13399	6
TCAACGC	8940	0.0	28.086018	4
TATCAAC	9820	0.0	26.57467	2
TGATCGC	1135	0.0	25.258358	9
GTACATG	16820	0.0	25.110907	1
ACGCAGA	9915	0.0	25.029531	7
TACATGG	16670	0.0	24.624365	2
CGCAGAG	10150	0.0	24.35666	8
ACATGGG	16390	0.0	23.954876	3
GTGATCG	1310	0.0	21.885508	8
GTACTAG	2005	0.0	21.347109	1
CATGGGG	11070	0.0	20.345778	4
TAACGCC	780	0.0	19.89325	4
GCAGAGT	12375	0.0	19.292551	9
GTATAAG	2665	0.0	19.060688	1
CTAACGC	765	0.0	19.054022	3
>>END_MODULE