FastQCFastQC Report
Mon 16 Jan 2017
ERR1146595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847920
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179950.3711901186488226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162500.3351953002524794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91300.18832819023416228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92050.059.3208731
GTATCAA181900.044.49871
ATCAACG224000.035.671193
CAACGCA226350.035.1944925
TATCAAC236350.034.0875972
TCAACGC236650.033.7445344
AACGCAG240750.033.2839286
ACGCAGA264100.030.1273257
CGCAGAG268450.029.6041268
GCAGAGT297850.026.5081399
GTACTAG19800.024.9415381
GTACATG170550.024.2677821
GTGGTAT29800.023.5163081
GAGTACT210850.022.94670312-13
AGAGTAC279000.022.93406310-11
ACATGGG164850.022.8515593
TACATGG172800.022.8084052
CAGAGTA290150.022.57105810-11
GTACTTT226600.021.25844614-15
AGTACTT223250.021.14589312-13
Produced by FastQC (version 0.11.5)