FastQCFastQC Report
Mon 16 Jan 2017
ERR1146594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240973
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.044.908471
GTATCAA58650.039.933021
CAACGCA64350.033.974615
ATCAACG65400.033.861013
TATCAAC73350.031.6012022
AACGCAG77150.029.544376
TCAACGC81400.027.2052844
ACGCAGA81500.027.0439917
CGCAGAG82750.026.577458
GTACATG121500.025.3533821
TACATGG121200.024.9051512
CTATTGA22300.024.6552689
GGGCTAT25550.023.5444246
GCTATTG23450.023.2461228
TCTAACG8100.023.2183722
CTAACGC7700.023.2032953
GCAGAGT91150.023.1482649
ACATGGG126250.023.1268773
TAACGCC8000.022.333174
GTCCTAC13700.022.3132921
Produced by FastQC (version 0.11.5)