##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146594_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3240973 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.291455991765435 31.0 31.0 34.0 28.0 34.0 2 31.450321246119607 33.0 31.0 34.0 28.0 34.0 3 31.452713428960994 33.0 31.0 34.0 28.0 34.0 4 34.91169503726196 37.0 35.0 37.0 32.0 37.0 5 34.97405624792308 37.0 35.0 37.0 32.0 37.0 6 34.97452647707957 37.0 35.0 37.0 32.0 37.0 7 34.929767387756705 37.0 35.0 37.0 32.0 37.0 8 34.92522862732889 37.0 35.0 37.0 32.0 37.0 9 36.4776290947194 39.0 37.0 39.0 32.0 39.0 10-11 36.47470744125298 39.0 37.0 39.0 32.0 39.0 12-13 36.43035100878656 39.0 37.0 39.0 32.0 39.0 14-15 37.77080324334698 40.0 38.0 41.0 32.0 41.0 16-17 37.797956663014475 40.0 38.0 41.0 32.0 41.0 18-19 37.820758765963184 40.0 38.0 41.0 32.0 41.0 20-21 37.92603610088699 40.0 38.0 41.0 32.0 41.0 22-23 37.941356037214746 40.0 38.0 41.0 32.0 41.0 24-25 37.963838328798175 40.0 38.0 41.0 32.0 41.0 26-27 38.02775632502955 40.0 38.0 41.0 32.0 41.0 28-29 38.019286492050384 40.0 38.0 41.0 32.0 41.0 30-31 37.99196198178757 40.0 38.0 41.0 32.0 41.0 32-33 37.95748175007938 40.0 38.0 41.0 31.5 41.0 34-35 37.90591467438945 40.0 38.0 41.0 31.0 41.0 36-37 37.829068461847726 40.0 38.0 41.0 31.0 41.0 38-39 37.765316002324 40.0 38.0 41.0 31.0 41.0 40-41 37.700990720996444 40.0 38.0 41.0 31.0 41.0 42-43 37.5352253783046 40.0 37.0 41.0 30.0 41.0 44-45 37.39796736967571 40.0 37.0 41.0 30.0 41.0 46-47 37.27524527356445 40.0 36.5 41.0 30.0 41.0 48-49 37.15272327168415 40.0 36.0 41.0 30.0 41.0 50-51 37.004480444607225 40.0 36.0 41.0 30.0 41.0 52-53 36.856299481668 39.5 35.0 41.0 30.0 41.0 54-55 36.730084761582404 39.0 35.0 41.0 30.0 41.0 56-57 36.526987882959844 39.0 35.0 41.0 30.0 41.0 58-59 36.313381968933406 39.0 35.0 41.0 29.5 41.0 60-61 36.08978661655003 38.0 35.0 40.0 29.0 41.0 62-63 35.846897829756685 37.5 35.0 40.0 29.0 41.0 64-65 35.54605453362308 37.0 34.5 40.0 29.0 41.0 66-67 35.310433008852584 36.5 34.0 40.0 29.0 41.0 68-69 35.05578602475244 36.0 34.0 39.0 28.0 41.0 70-71 34.79868406802525 35.5 34.0 39.0 28.0 41.0 72-73 34.477100241193 35.0 34.0 39.0 28.0 40.5 74-75 34.155389600592166 35.0 34.0 37.5 28.0 40.0 76-77 33.901447034578815 35.0 34.0 37.0 27.0 39.0 78-79 33.60932596476428 35.0 34.0 36.5 27.0 39.0 80-81 33.391959760232496 35.0 33.5 36.0 27.0 39.0 82-83 33.13811238168291 35.0 33.0 36.0 27.0 37.0 84-85 32.95226263841137 35.0 33.0 35.5 26.5 37.0 86-87 32.760771070909875 35.0 33.0 35.0 26.0 37.0 88-89 32.601868327813904 35.0 33.0 35.0 26.0 36.0 90-91 32.46699972508256 35.0 33.0 35.0 26.0 36.0 92-93 32.322243042444356 35.0 33.0 35.0 25.5 36.0 94-95 32.209622233816816 35.0 33.0 35.0 25.0 35.5 96-97 32.117815853448946 35.0 33.0 35.0 25.0 35.0 98-99 32.02363950579039 35.0 33.0 35.0 25.0 35.0 100 30.86438949044006 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23043446049733518 1101 2 0.261647789101346 1101 3 0.25880681057651955 1101 4 0.21325173922137708 1101 5 0.11458841988888224 1101 6 0.281526834348206 1101 7 0.24433182044415247 1101 8 0.2556614581512662 1101 9 0.35666842439707125 1101 10-11 0.37154274218664085 1101 12-13 0.42209715528309033 1101 14-15 0.4679445930536943 1101 16-17 0.4939450919212973 1101 18-19 0.408087896760712 1101 20-21 0.4360311167713746 1101 22-23 0.48864205545685735 1101 24-25 0.5042205198954264 1101 26-27 0.43974001127944007 1101 28-29 0.4047965930254023 1101 30-31 0.48958820165548644 1101 32-33 0.5589438519997287 1101 34-35 0.5162268351425183 1101 36-37 0.5178285810403978 1101 38-39 0.5482618772166177 1101 40-41 0.5130386273075658 1101 42-43 0.4982346652182983 1101 44-45 0.40020274998676797 1101 46-47 0.4359329581612954 1101 48-49 0.4482360380703483 1101 50-51 0.45608090871576223 1101 52-53 0.41326549668639956 1101 54-55 0.3713491886814566 1101 56-57 0.3663495280241733 1101 58-59 0.3758865748396403 1101 60-61 0.41710789481846433 1101 62-63 0.4109972241328208 1101 64-65 0.37913823726142226 1101 66-67 0.3471817115825786 1101 68-69 0.32994909692407504 1101 70-71 0.2999787350287022 1101 72-73 0.3386020724023098 1101 74-75 0.3420357371734113 1101 76-77 0.2988686489841115 1101 78-79 0.3700042705209441 1101 80-81 0.31242277915304584 1101 82-83 0.3585516284064951 1101 84-85 0.17380122529137054 1101 86-87 0.2516510753661265 1101 88-89 0.2335497281263983 1101 90-91 0.28744840795118165 1101 92-93 0.3170936443684198 1101 94-95 0.3587642165448912 1101 96-97 0.3233324708648624 1101 98-99 0.37628088382115976 1101 100 0.34902513983487893 1102 1 0.2082775650448596 1102 2 0.2270222102453232 1102 3 0.19003306580962587 1102 4 0.22866615230671528 1102 5 0.2657973218500089 1102 6 0.2913838623688463 1102 7 0.2188799149496532 1102 8 0.27155466133449124 1102 9 0.33344796537026156 1102 10-11 0.33011517648942856 1102 12-13 0.39755560512210764 1102 14-15 0.41005741273315977 1102 16-17 0.4063230898178034 1102 18-19 0.3490762138576997 1102 20-21 0.34634241009509736 1102 22-23 0.33736274656259013 1102 24-25 0.4166822085107498 1102 26-27 0.3341859887626484 1102 28-29 0.36375772868520784 1102 30-31 0.3854032697835166 1102 32-33 0.37964132132665895 1102 34-35 0.36584758162700126 1102 36-37 0.43559313968216884 1102 38-39 0.4037959453642159 1102 40-41 0.3443538580830676 1102 42-43 0.29998799295007217 1102 44-45 0.29985379096216747 1102 46-47 0.31274429414806093 1102 48-49 0.3048712832761211 1102 50-51 0.3061273675368028 1102 52-53 0.3117688530084237 1102 54-55 0.20744325418032616 1102 56-57 0.2875010790338237 1102 58-59 0.26880554816538904 1102 60-61 0.25123449681289145 1102 62-63 0.27463540571510947 1102 64-65 0.3612225992274176 1102 66-67 0.30004742582190147 1102 68-69 0.2748726853181509 1102 70-71 0.27794773428796304 1102 72-73 0.28744070516277986 1102 74-75 0.31806226251207903 1102 76-77 0.32176728463316806 1102 78-79 0.3573604018143257 1102 80-81 0.2655992421669424 1102 82-83 0.2480514184193936 1102 84-85 0.25191545104376445 1102 86-87 0.18970622929994363 1102 88-89 0.2408257711029762 1102 90-91 0.17713285119214817 1102 92-93 0.16168052465901894 1102 94-95 0.13302050960206913 1102 96-97 0.19065112179917065 1102 98-99 0.18177174667103912 1102 100 0.2095460214582161 1103 1 0.1556387649267883 1103 2 0.2133635014698072 1103 3 0.18078012719362135 1103 4 0.18701705863864504 1103 5 0.16238145299345064 1103 6 0.17505777772633735 1103 7 0.2278199615951948 1103 8 0.23610156944489802 1103 9 0.2855502113162345 1103 10-11 0.29210339158969845 1103 12-13 0.3031838118420893 1103 14-15 0.3558494827333547 1103 16-17 0.38386290544182344 1103 18-19 0.37472127777349584 1103 20-21 0.31686687533244395 1103 22-23 0.355126652742662 1103 24-25 0.39520120047362894 1103 26-27 0.38924974191068884 1103 28-29 0.3573156646674889 1103 30-31 0.3912284873303946 1103 32-33 0.4315619836463327 1103 34-35 0.42276114952785804 1103 36-37 0.37737236613527614 1103 38-39 0.3643520717906128 1103 40-41 0.3371066165808685 1103 42-43 0.3039046177877651 1103 44-45 0.32188843903761466 1103 46-47 0.3388704516108234 1103 48-49 0.3570438051129372 1103 50-51 0.3202772345778442 1103 52-53 0.28885720561689965 1103 54-55 0.2619557023047605 1103 56-57 0.35416695662655684 1103 58-59 0.32900496486261943 1103 60-61 0.31540617611198485 1103 62-63 0.31436487574245575 1103 64-65 0.25607906104244194 1103 66-67 0.26278960911862725 1103 68-69 0.20171546684563424 1103 70-71 0.23048228002534898 1103 72-73 0.22008519305460084 1103 74-75 0.23080662783978312 1103 76-77 0.23808720161614616 1103 78-79 0.27940553330061135 1103 80-81 0.28389488755775716 1103 82-83 0.17344037148092895 1103 84-85 0.17291336869376295 1103 86-87 0.22182564485059686 1103 88-89 0.1386752386632608 1103 90-91 0.28762808204538715 1103 92-93 0.20594080890921873 1103 94-95 0.22922319596402474 1103 96-97 0.23585273904645732 1103 98-99 0.223687354086465 1103 100 0.3558145632866214 1104 1 0.20870272110562382 1104 2 0.24798101408726225 1104 3 0.1279843663951219 1104 4 0.1161450516692426 1104 5 0.15580551249690444 1104 6 0.22682433365014987 1104 7 0.24391377650252366 1104 8 0.23955591165618273 1104 9 0.23621073173671192 1104 10-11 0.31989230590509266 1104 12-13 0.3447545805458674 1104 14-15 0.3767502763130821 1104 16-17 0.3551318119787439 1104 18-19 0.3470952911311116 1104 20-21 0.38638389862089184 1104 22-23 0.3387494110392808 1104 24-25 0.42719633274788293 1104 26-27 0.3796019029577309 1104 28-29 0.3998578762272089 1104 30-31 0.4098144373928818 1104 32-33 0.4453709841424711 1104 34-35 0.39988409889328125 1104 36-37 0.3324276474998342 1104 38-39 0.2963585472598922 1104 40-41 0.33574954081266384 1104 42-43 0.3065354175064883 1104 44-45 0.30784517579583337 1104 46-47 0.21474972143971627 1104 48-49 0.2539966443126076 1104 50-51 0.2105526670492779 1104 52-53 0.2600494192967773 1104 54-55 0.2649593783061661 1104 56-57 0.2993834322933111 1104 58-59 0.2479719197321657 1104 60-61 0.24926726141644195 1104 62-63 0.22121083836869815 1104 64-65 0.2687647637640893 1104 66-67 0.28502576668299895 1104 68-69 0.24182075394302416 1104 70-71 0.26939410788555307 1104 72-73 0.21339357544100324 1104 74-75 0.23478687486221617 1104 76-77 0.2681347282255828 1104 78-79 0.2633554736327568 1104 80-81 0.24938787286574637 1104 82-83 0.19105679218191085 1104 84-85 0.12593912131831786 1104 86-87 0.12912729979876758 1104 88-89 0.21475695110097348 1104 90-91 0.15428267583781263 1104 92-93 0.19186575614652668 1104 94-95 0.21479973413418207 1104 96-97 0.15328614293415654 1104 98-99 0.1731787294057412 1104 100 0.26629231284163524 1105 1 0.03796159072537719 1105 2 0.027392587271947377 1105 3 0.03637619173442985 1105 4 0.11557499137268934 1105 5 0.035734532265053076 1105 6 4.4666523736935915E-4 1105 7 0.0657353697150782 1105 8 0.14882828009776006 1105 9 0.07441898349815546 1105 10-11 0.08734001592109308 1105 12-13 0.057523396385747105 1105 14-15 0.1533583680297994 1105 16-17 0.19516007730338458 1105 18-19 0.15481846426538937 1105 20-21 0.1497222176709485 1105 22-23 0.18050476791995607 1105 24-25 0.20023283230432298 1105 26-27 0.17563391289318275 1105 28-29 0.20894836215755674 1105 30-31 0.25257304493444366 1105 32-33 0.25592742429616067 1105 34-35 0.2761417709011198 1105 36-37 0.17034750183536573 1105 38-39 0.2759504431139561 1105 40-41 0.2126949049998359 1105 42-43 0.14933250114693664 1105 44-45 0.1423123078235662 1105 46-47 0.1766064878479554 1105 48-49 0.16958961571477005 1105 50-51 0.16780980648255195 1105 52-53 0.19703435889994836 1105 54-55 0.1683092748490438 1105 56-57 0.16590286309463664 1105 58-59 0.13373068746700767 1105 60-61 0.2539261376221944 1105 62-63 0.1535973394478347 1105 64-65 0.30456343126300567 1105 66-67 0.18977357863342093 1105 68-69 0.19225021801850772 1105 70-71 0.16492280885174182 1105 72-73 0.15979810284483165 1105 74-75 0.11016385521143235 1105 76-77 0.11367629169716054 1105 78-79 0.14387800084394797 1105 80-81 0.11007839751979276 1105 82-83 0.1517151296232413 1105 84-85 0.21457384126852475 1105 86-87 0.2145434470339751 1105 88-89 0.27613648018223813 1105 90-91 0.18275372628730935 1105 92-93 0.19593058020254972 1105 94-95 0.19406710860591403 1105 96-97 0.20399516533175444 1105 98-99 0.20369923872629414 1105 100 0.33010806729380704 1106 1 0.06056597565323685 1106 2 0.11687263361061184 1106 3 0.12425913416975476 1106 4 0.09742912991374197 1106 5 0.11111046295933846 1106 6 0.2015135451868062 1106 7 0.1635129361987353 1106 8 0.21220891923295682 1106 9 0.21691993373909213 1106 10-11 0.2263689221221128 1106 12-13 0.24605175691193182 1106 14-15 0.29839093257103144 1106 16-17 0.3290604790030258 1106 18-19 0.30445189615345214 1106 20-21 0.3139465728762545 1106 22-23 0.3012057346138661 1106 24-25 0.2942855009374199 1106 26-27 0.21696789036285224 1106 28-29 0.21073076012987713 1106 30-31 0.21417967571684926 1106 32-33 0.24854728574159424 1106 34-35 0.26466887298438024 1106 36-37 0.2911578132370707 1106 38-39 0.2362900083032784 1106 40-41 0.19911656215010964 1106 42-43 0.29797374400381926 1106 44-45 0.18631604866170193 1106 46-47 0.25930452889509326 1106 48-49 0.23243292302396412 1106 50-51 0.21817183021724418 1106 52-53 0.2304342265770103 1106 54-55 0.24529745737080333 1106 56-57 0.3763331876540619 1106 58-59 0.34892167832069276 1106 60-61 0.2982709397984564 1106 62-63 0.31236527255087054 1106 64-65 0.3603646783799448 1106 66-67 0.34283778229423234 1106 68-69 0.3075855802414935 1106 70-71 0.2787870046822434 1106 72-73 0.2968405335786173 1106 74-75 0.3103471917025189 1106 76-77 0.33143443621161595 1106 78-79 0.3019312548860569 1106 80-81 0.2473653157491995 1106 82-83 0.23091786874080356 1106 84-85 0.28781757346533965 1106 86-87 0.24677039929261468 1106 88-89 0.26757673781813907 1106 90-91 0.22763206165628702 1106 92-93 0.23004065247679506 1106 94-95 0.20486205535163293 1106 96-97 0.2190495452141903 1106 98-99 0.2768517571152884 1106 100 0.18370892695015328 1107 1 0.0748099165541376 1107 2 0.07176123839667525 1107 3 0.04674121457201963 1107 4 0.15952434132358206 1107 5 0.135701871294593 1107 6 0.138309397017359 1107 7 0.09155071936764614 1107 8 0.137821304973464 1107 9 0.23356321133306324 1107 10-11 0.19545492141183018 1107 12-13 0.17186692228347056 1107 14-15 0.21248733735266256 1107 16-17 0.28996141786288376 1107 18-19 0.2522056094365581 1107 20-21 0.23465545198740756 1107 22-23 0.22653326689747644 1107 24-25 0.3092311577453444 1107 26-27 0.29522237554220965 1107 28-29 0.24463713230323947 1107 30-31 0.2674875220008417 1107 32-33 0.29152749436215686 1107 34-35 0.32991773945170877 1107 36-37 0.2844046284271897 1107 38-39 0.2801084674719263 1107 40-41 0.24467184252295482 1107 42-43 0.27437450196893565 1107 44-45 0.25988968033973237 1107 46-47 0.29362896598539834 1107 48-49 0.301452878463607 1107 50-51 0.278922268330561 1107 52-53 0.23677140159504262 1107 54-55 0.1741343999848013 1107 56-57 0.16330615628751843 1107 58-59 0.19996592673827251 1107 60-61 0.24131487478759084 1107 62-63 0.19916181088066054 1107 64-65 0.28177017931144377 1107 66-67 0.12508761432054882 1107 68-69 0.2211271231087366 1107 70-71 0.1615208449056169 1107 72-73 0.20503778920827642 1107 74-75 0.22370552235326358 1107 76-77 0.26030605359594716 1107 78-79 0.19832358771278535 1107 80-81 0.16061680175769055 1107 82-83 0.19788494659864853 1107 84-85 0.19854770519361153 1107 86-87 0.1992681939684786 1107 88-89 0.1870182169065373 1107 90-91 0.16099775665106364 1107 92-93 0.24311817870434282 1107 94-95 0.23413796831472666 1107 96-97 0.22624451633181053 1107 98-99 0.2803626104058594 1107 100 0.2653565985764921 1108 1 0.03364002681449563 1108 2 0.027125773152224752 1108 3 0.04240165428284115 1108 4 0.03827201053628215 1108 5 0.010011445604035885 1108 6 0.007847170848329199 1108 7 -0.05231490582922049 1108 8 0.08468700847654276 1108 9 0.01588506701698833 1108 10-11 0.044196571728257084 1108 12-13 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-0.42008347162693127 2214 96-97 -0.4348102651243977 2214 98-99 -0.3937766082641545 2214 100 -0.4213797954685141 2215 1 -0.40454884104257616 2215 2 -0.3883544865606545 2215 3 -0.4359428421084708 2215 4 -0.4503448513312449 2215 5 -0.41752512173811596 2215 6 -0.4474233316565943 2215 7 -0.41297292024969323 2215 8 -0.40217454689880583 2215 9 -0.6045363189675186 2215 10-11 -0.5414749768446327 2215 12-13 -0.5596647570568365 2215 14-15 -0.6723337117171795 2215 16-17 -0.6874811523719728 2215 18-19 -0.8047800355111576 2215 20-21 -0.7418432576783758 2215 22-23 -0.6595950413420653 2215 24-25 -0.5823332570792701 2215 26-27 -0.6685780429853025 2215 28-29 -0.6039913340819894 2215 30-31 -0.6395245635598812 2215 32-33 -0.6244236584055045 2215 34-35 -0.6274678862224334 2215 36-37 -0.6361953345869082 2215 38-39 -0.733339838000397 2215 40-41 -0.7287894941540003 2215 42-43 -0.7748529529837711 2215 44-45 -0.6766008622789812 2215 46-47 -0.6808942348617748 2215 48-49 -0.5826259289644753 2215 50-51 -0.6424758593321656 2215 52-53 -0.6024583992975181 2215 54-55 -0.6639683941735797 2215 56-57 -0.6535058204689719 2215 58-59 -0.5978481768421773 2215 60-61 -0.5357273311334367 2215 62-63 -0.5504760354954072 2215 64-65 -0.6131121656808887 2215 66-67 -0.5136722045077562 2215 68-69 -0.6152912848877534 2215 70-71 -0.528361047857338 2215 72-73 -0.4576733958507049 2215 74-75 -0.5047315150706027 2215 76-77 -0.5345266000025433 2215 78-79 -0.46158963000954856 2215 80-81 -0.4800810756445415 2215 82-83 -0.49434551056486953 2215 84-85 -0.44333468340654036 2215 86-87 -0.47887336674507 2215 88-89 -0.44246312715144853 2215 90-91 -0.5118659777567771 2215 92-93 -0.4850010195360568 2215 94-95 -0.485880368509477 2215 96-97 -0.4286889965294023 2215 98-99 -0.42226017188688303 2215 100 -0.3549437490209968 2216 1 -0.21558238090607418 2216 2 -0.24213655956773295 2216 3 -0.1615564755947041 2216 4 -0.07856513985714741 2216 5 -0.0976550080977745 2216 6 -0.12782289420234605 2216 7 -0.1893458092946645 2216 8 -0.21088091524518404 2216 9 -0.287524189981923 2216 10-11 -0.360864490091906 2216 12-13 -0.2857810293199421 2216 14-15 -0.472596490042541 2216 16-17 -0.4703903397088709 2216 18-19 -0.43918827780534286 2216 20-21 -0.3683636504954322 2216 22-23 -0.4551894917190893 2216 24-25 -0.42017298822530336 2216 26-27 -0.5443619140381557 2216 28-29 -0.526745776171353 2216 30-31 -0.5106235395266978 2216 32-33 -0.5346170398544388 2216 34-35 -0.5361400311574727 2216 36-37 -0.4391924831984326 2216 38-39 -0.3921365688337062 2216 40-41 -0.37600651159060305 2216 42-43 -0.3620219195850325 2216 44-45 -0.3217633245401714 2216 46-47 -0.31284933914577095 2216 48-49 -0.18332697455539915 2216 50-51 -0.2567530351137961 2216 52-53 -0.2641141118818169 2216 54-55 -0.2669294479008073 2216 56-57 -0.2451652483872948 2216 58-59 -0.213549487302771 2216 60-61 -0.24479358986672395 2216 62-63 -0.26265364359751686 2216 64-65 -0.29309578569046835 2216 66-67 -0.17807008518222744 2216 68-69 -0.28741101129681823 2216 70-71 -0.3036490450478766 2216 72-73 -0.2387975763654282 2216 74-75 -0.13687726927113886 2216 76-77 -0.2258996669908484 2216 78-79 -0.22901273650331433 2216 80-81 -0.23745859080705856 2216 82-83 -0.30798796555286856 2216 84-85 -0.19447766250925014 2216 86-87 -0.24330027570373858 2216 88-89 -0.23507682220198944 2216 90-91 -0.2087007545105095 2216 92-93 -0.20052319526347873 2216 94-95 -0.24321674705998575 2216 96-97 -0.29583635221991855 2216 98-99 -0.2361693373387972 2216 100 -0.3453440642465857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 964.0 1 237.0 2 177.0 3 179.0 4 129.0 5 188.0 6 357.0 7 969.0 8 2815.0 9 3939.0 10 4133.0 11 4476.0 12 5151.0 13 6082.0 14 7848.0 15 8944.0 16 9439.0 17 10303.0 18 11573.0 19 12622.0 20 14458.0 21 16089.0 22 17680.0 23 20608.0 24 25475.0 25 31073.0 26 32702.0 27 30217.0 28 32893.0 29 39937.0 30 45706.0 31 52696.0 32 66692.0 33 88821.0 34 133293.0 35 208091.0 36 388132.0 37 781956.0 38 851441.0 39 272109.0 40 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85570952145931 20.584519992266358 13.30757717735674 24.252193308917594 2 15.162187588090054 20.962939886806307 40.1087353533158 23.76613717178784 3 17.581897707074187 26.95222319804046 30.53088217674168 24.934996918143675 4 12.507083347101148 17.830209043276433 39.71565939283479 29.947048216787632 5 13.249612518964875 38.63554177707959 35.124202378897316 12.990643325058219 6 29.149738930689008 39.08569070876141 18.501162281128426 13.263408079421154 7 25.74984404627348 33.48280185538612 23.386388482276864 17.38096561606354 8 25.306275095719656 35.1754515453806 21.13948232637514 18.378791032524607 9 26.335855116793432 15.685419015303307 21.1230165310054 36.85570933689786 10-11 24.411021632839415 26.675221459880454 28.585443686357255 20.32831322092288 12-13 24.765022402417255 24.837566650703536 28.001539500412665 22.395871446466543 14-15 22.604139566589186 25.93714609067953 25.531125492433336 25.92758885029795 16-17 22.103557282227364 28.495607166998678 26.533291871799868 22.86754367897409 18-19 21.965633588920088 28.603086958528923 27.639437240793235 21.791842211757753 20-21 23.497314834756388 27.53949010807819 26.82551482070723 22.13768023645819 22-23 23.69948925530951 27.320047064904763 26.324387491579838 22.65607618820589 24-25 23.119767912229076 27.501428161176868 26.885666820033165 22.493137106560894 26-27 22.819244326644945 27.825376515138416 26.566517880300665 22.78886127791597 28-29 22.56352376438037 28.05095488295755 26.596203936875355 22.78931741578672 30-31 22.960572068783637 27.717147611089082 26.612185984117158 22.71009433601012 32-33 23.107470985900356 27.96318102185588 26.06082391674055 22.86852407550321 34-35 23.25929540308067 27.756943323885896 26.648914445773244 22.334846827260186 36-37 23.15829100646584 27.572324204400555 26.59756838361344 22.67181640552016 38-39 23.093663919077233 27.556657933531305 26.782550179498436 22.56712796789303 40-41 22.960062380816353 27.1591714468798 27.060628285994426 22.820137886309418 42-43 22.79126571148769 27.341439785151127 26.787007523478252 23.08028697988293 44-45 22.65202882151954 27.835967976638408 26.775095301741498 22.73690790010055 46-47 22.80175342161174 27.461800237165868 26.612280127476655 23.124166213745738 48-49 23.014907160894403 27.088129012870986 26.890318089538706 23.0066457366959 50-51 22.869163220819523 27.291866019842338 26.49562170628149 23.343349053056652 52-53 22.602426970900467 27.605415786660785 26.548559415510866 23.24359782692788 54-55 22.609080332072068 27.42415621253168 26.63940317820115 23.327360277195098 56-57 23.005785630998517 27.239100346501655 26.465351147359172 23.289762875140656 58-59 22.547730903855008 27.28125722908888 26.955571814946445 23.215440052109663 60-61 22.740254156935393 27.579140674560403 26.664024989483735 23.016580179020472 62-63 22.942562212237757 27.178360665357655 26.442600432545788 23.4364766898588 64-65 22.43979736432787 27.262741326335842 26.627620816052715 23.66984049328357 66-67 22.928655704084406 27.12488572063947 26.466358627165132 23.480099948110993 68-69 22.526264097214607 27.10078031740835 26.681187910871166 23.691767674505872 70-71 22.58234139439597 27.054525485414217 26.687909981222923 23.67522313896689 72-73 22.892383603055215 27.133057972355086 26.775686841462772 23.19887158312693 74-75 22.831697498281184 27.435884923034727 26.663699534298175 23.06871804438591 76-77 22.809754123929444 27.32836392499869 26.493401404035446 23.368480547036423 78-79 22.677070333318767 27.3301032423481 26.557256639341187 23.43556978499195 80-81 22.557301879933448 27.41460737351983 26.80606204178804 23.22202870475869 82-83 22.986228611352548 26.76619464773402 27.259247796658126 22.98832894425531 84-85 23.130442705558412 27.242475766013275 26.449305912675804 23.177775615752516 86-87 22.51000599479463 27.42162987378193 26.806491673844874 23.261872457578566 88-89 22.945210556387728 26.7372021557515 26.675093040134655 23.642494247726116 90-91 22.917466452244344 26.737211486521755 26.823318087605745 23.522003973628156 92-93 23.177123907381496 26.727084987178184 26.75506491513141 23.340726190308906 94-95 22.474742678515263 26.810430381638913 27.0649839724659 23.64984296737993 96-97 22.625629442344692 26.87500897521359 26.829812501804696 23.669549080637026 98-99 22.53041705217403 27.14186501088069 26.63173664947655 23.69598128746873 100 23.136541454591345 26.830178081412182 26.198684370058967 23.834596093937513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1676.0 1 1150.0 2 646.5 3 651.5 4 623.5 5 610.5 6 576.5 7 547.5 8 537.5 9 534.0 10 477.0 11 447.0 12 495.0 13 519.0 14 540.5 15 661.0 16 783.0 17 867.5 18 1042.5 19 1292.0 20 1719.5 21 2319.5 22 3370.0 23 4864.0 24 6702.0 25 8830.5 26 12076.0 27 16361.5 28 20197.0 29 25585.5 30 33554.0 31 42241.0 32 51012.0 33 58104.5 34 65488.0 35 72642.5 36 80847.0 37 92369.5 38 97783.0 39 97303.5 40 95825.5 41 94123.5 42 95667.5 43 101327.0 44 107909.5 45 118065.5 46 128510.0 47 134575.0 48 136506.5 49 132065.0 50 126226.5 51 126070.5 52 126504.5 53 124647.0 54 119977.0 55 109023.5 56 99543.0 57 86877.0 58 73941.0 59 65670.0 60 54504.0 61 42481.0 62 32983.5 63 26338.0 64 19819.5 65 13592.5 66 9610.0 67 7302.5 68 5541.5 69 4184.5 70 3377.0 71 2610.0 72 2065.5 73 1764.5 74 1302.0 75 881.0 76 672.0 77 446.0 78 287.5 79 233.5 80 143.5 81 90.5 82 72.0 83 54.0 84 43.5 85 32.0 86 31.5 87 31.0 88 27.5 89 22.5 90 20.0 91 17.0 92 15.0 93 15.5 94 13.0 95 12.0 96 14.5 97 15.0 98 12.0 99 13.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09784098787617176 2 0.06479535620938526 3 0.08222839252286274 4 0.08466593211359674 5 0.08485106170276642 6 0.09083691841925248 7 0.08741202101961355 8 0.08858450841768814 9 0.07380499621564265 10-11 0.08122560724819367 12-13 0.06973214525390986 14-15 0.07992970012400598 16-17 0.08114846991937298 18-19 0.08045423395998672 20-21 0.07237024189957769 22-23 0.07610368861449941 24-25 0.07846409087641273 26-27 0.07173771580324798 28-29 0.08077821074103363 30-31 0.08519046594957748 32-33 0.08657893786835003 34-35 0.0888776302672068 36-37 0.08886220280144265 38-39 0.0901735373913945 40-41 0.0863320984161238 42-43 0.0918242762281574 44-45 0.08753544074572667 46-47 0.08432652786678568 48-49 0.09356757985950516 50-51 0.08937130917165925 52-53 0.0946783573945232 54-55 0.08702633437551006 56-57 0.09372185451714654 58-59 0.09830381184909594 60-61 0.0966067906150406 62-63 0.09767128575276622 64-65 0.10000083308315127 66-67 0.10160528952262175 68-69 0.09836552171215249 70-71 0.10023224506961335 72-73 0.09757872095818138 74-75 0.10117332048122586 76-77 0.09759414842394552 78-79 0.1047987749357986 80-81 0.10034023732996232 82-83 0.10462907281239307 84-85 0.10083391623441479 86-87 0.0976250033554738 88-89 0.09635995116281439 90-91 0.0964525159573992 92-93 0.09063636136431868 94-95 0.09106833040571458 96-97 0.08912446971943301 98-99 0.10356457767466745 100 0.24205693783934643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3240973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.49339020782142 #Duplication Level Percentage of deduplicated Percentage of total 1 66.89169690045523 33.77588553258005 2 15.716882475307075 15.871973593523009 3 6.858356378972717 10.389049944833118 4 3.483577012527863 7.035904536502648 5 2.0928763839678797 5.283821195621222 6 1.3025697551529563 3.946269775190678 7 0.839789964718758 2.968268966680978 8 0.5945637036506638 2.4017229673472373 9 0.4193166201493885 1.9055445949645118 >10 1.7471680293823042 14.043869105226072 >50 0.04119166323898082 1.3959776019608812 >100 0.01190486997618645 0.901995061677807 >500 0.0 0.0 >1k 1.0624249988774044E-4 0.07971712389180347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.08549315282787E-5 0.0 22-23 0.0 0.0 0.0 2.776943837545083E-4 0.0 24-25 0.0 0.0 0.0 7.405183566786888E-4 0.0 26-27 0.0 0.0 0.0 0.0017124486998194677 0.0 28-29 0.0 0.0 0.0 0.005368758085920494 0.0 30-31 0.0 0.0 0.0 0.017463891245005744 0.0 32-33 0.0 0.0 0.0 0.04571158105914489 0.0 34-35 0.0 0.0 0.0 0.09106833040571458 0.0 36-37 0.0 0.0 0.0 0.16334600751070744 0.0 38-39 0.0 0.0 0.0 0.2680522176519212 0.0 40-41 0.0 0.0 0.0 0.38102754944271366 0.0 42-43 0.0 0.0 0.0 0.5010378056219537 0.0 44-45 0.0 0.0 0.0 0.6562535386749596 0.0 46-47 0.0 0.0 0.0 0.8426327525715271 0.0 48-49 0.0 0.0 0.0 1.0398729023660487 0.0 50-51 0.0 0.0 0.0 1.2476500112774775 0.0 52-53 0.0 0.0 0.0 1.4597776655343935 0.0 54-55 0.0 0.0 0.0 1.6903719963109842 0.0 56-57 0.0 0.0 0.0 1.966554488420607 0.0 58-59 0.0 0.0 0.0 2.2845608402168116 0.0 60-61 0.0 0.0 0.0 2.6163902013376847 0.0 62-63 0.0 0.0 0.0 2.9517061697212537 0.0 64-65 0.0 0.0 0.0 3.307710369694533 3.08549315282787E-5 66-67 0.0 0.0 0.0 3.7097038451107123 3.08549315282787E-5 68-69 0.0 0.0 0.0 4.156112994461848 3.08549315282787E-5 70-71 0.0 0.0 0.0 4.636956247398544 3.08549315282787E-5 72-73 0.0 0.0 0.0 5.130496304659125 3.08549315282787E-5 74-75 0.0 0.0 0.0 5.631703812404485 3.08549315282787E-5 76-77 0.0 0.0 0.0 6.180011373127762 3.08549315282787E-5 78-79 0.0 0.0 0.0 6.7834875514236 3.08549315282787E-5 80-81 0.0 0.0 0.0 7.433847798176659 3.08549315282787E-5 82-83 0.0 0.0 0.0 8.100561158639705 3.08549315282787E-5 84-85 0.0 0.0 0.0 8.765284376019178 3.08549315282787E-5 86-87 0.0 0.0 0.0 9.46404058287434 3.08549315282787E-5 88 0.0 0.0 0.0 10.013659478187568 3.08549315282787E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3215 0.0 44.90847 1 GTATCAA 5865 0.0 39.93302 1 CAACGCA 6435 0.0 33.97461 5 ATCAACG 6540 0.0 33.86101 3 TATCAAC 7335 0.0 31.601202 2 AACGCAG 7715 0.0 29.54437 6 TCAACGC 8140 0.0 27.205284 4 ACGCAGA 8150 0.0 27.043991 7 CGCAGAG 8275 0.0 26.57745 8 GTACATG 12150 0.0 25.353382 1 TACATGG 12120 0.0 24.905151 2 CTATTGA 2230 0.0 24.655268 9 GGGCTAT 2555 0.0 23.544424 6 GCTATTG 2345 0.0 23.246122 8 TCTAACG 810 0.0 23.218372 2 CTAACGC 770 0.0 23.203295 3 GCAGAGT 9115 0.0 23.148264 9 ACATGGG 12625 0.0 23.126877 3 TAACGCC 800 0.0 22.33317 4 GTCCTAC 1370 0.0 22.313292 1 >>END_MODULE