FastQCFastQC Report
Mon 16 Jan 2017
ERR1146594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240973
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115300.3557573605210534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109290.3372135466725579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58580.18074818889265662No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT37720.11638480172466725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.064.3280261
GTATCAA128300.043.8023381
ATCAACG162750.033.8643383
CAACGCA163800.033.659865
TATCAAC167300.033.451362
AACGCAG177550.031.7399756
TCAACGC180900.030.6746354
GTGGTAT15750.030.1576061
ACGCAGA194050.028.4096327
CGCAGAG196650.027.9862178
GCAGAGT212900.025.6289679
GTACATG122400.025.2429431
TACATGG121300.024.8519212
ACATGGG126150.023.5222053
CAGAGTA205250.023.08103610-11
GAGTACT144050.022.7405412-13
AGAGTAC202300.021.75653810-11
GTGATCG11550.021.5662758
TCTAACG8850.021.25592
CTATTGA26200.020.8080439
Produced by FastQC (version 0.11.5)