##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3240973
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34606150683761	33.0	31.0	34.0	30.0	34.0
2	32.45849903717186	34.0	31.0	34.0	30.0	34.0
3	32.55631996934254	34.0	31.0	34.0	31.0	34.0
4	36.053518187285114	37.0	35.0	37.0	35.0	37.0
5	36.09782093217068	37.0	35.0	37.0	35.0	37.0
6	36.156649561721125	37.0	36.0	37.0	35.0	37.0
7	36.12337375226514	37.0	36.0	37.0	35.0	37.0
8	36.13023866598086	37.0	36.0	37.0	35.0	37.0
9	37.92320948060968	39.0	38.0	39.0	35.0	39.0
10-11	37.873102460279675	39.0	38.0	39.0	35.0	39.0
12-13	37.83888742670797	39.0	38.0	39.0	35.0	39.0
14-15	39.18373340351802	40.0	39.0	41.0	36.0	41.0
16-17	39.13099661737385	40.0	39.0	41.0	36.0	41.0
18-19	39.24055785099104	40.5	39.0	41.0	36.0	41.0
20-21	39.29611446932758	41.0	39.0	41.0	36.0	41.0
22-23	39.25891221555996	41.0	39.0	41.0	36.0	41.0
24-25	39.237262852853135	41.0	39.0	41.0	36.0	41.0
26-27	39.15124562901326	41.0	39.0	41.0	35.0	41.0
28-29	39.0915050819615	40.5	39.0	41.0	35.0	41.0
30-31	38.93877363372049	40.0	38.5	41.0	35.0	41.0
32-33	38.81178862026928	40.0	38.0	41.0	35.0	41.0
34-35	38.72554893237309	40.0	38.0	41.0	35.0	41.0
36-37	38.64965999408202	40.0	38.0	41.0	35.0	41.0
38-39	38.548411850391844	40.0	38.0	41.0	35.0	41.0
40-41	38.43765992496698	40.0	38.0	41.0	34.0	41.0
42-43	38.31470687969323	40.0	38.0	41.0	34.0	41.0
44-45	38.127994586810814	40.0	38.0	41.0	33.5	41.0
46-47	37.83793046100662	40.0	37.0	41.0	33.0	41.0
48-49	37.501164619390536	40.0	37.0	41.0	32.5	41.0
50-51	37.3605239846182	40.0	37.0	41.0	32.0	41.0
52-53	37.24247209094306	40.0	36.0	41.0	32.0	41.0
54-55	37.066104685228794	39.5	36.0	41.0	32.0	41.0
56-57	36.884958313444756	39.0	35.0	41.0	31.5	41.0
58-59	36.675258633749806	39.0	35.0	41.0	31.0	41.0
60-61	36.45300994485298	39.0	35.0	41.0	31.0	41.0
62-63	36.1970358901478	38.0	35.0	41.0	31.0	41.0
64-65	35.91506578425677	37.0	35.0	40.0	31.0	41.0
66-67	35.640440694816036	37.0	35.0	40.0	30.0	41.0
68-69	35.351286480942605	36.0	35.0	39.5	30.0	41.0
70-71	35.062254144048715	36.0	35.0	39.0	30.0	41.0
72-73	34.75921737083277	35.0	35.0	39.0	29.5	41.0
74-75	34.46504136257846	35.0	34.5	37.5	29.0	40.0
76-77	34.19613508042184	35.0	34.0	37.0	29.0	39.0
78-79	33.93415449619605	35.0	34.0	37.0	29.0	39.0
80-81	33.70714751403359	35.0	34.0	36.0	29.0	39.0
82-83	33.47525341926638	35.0	34.0	36.0	29.0	37.0
84-85	33.28657566724561	35.0	34.0	35.5	29.0	37.0
86-87	33.11691334670175	35.0	34.0	35.0	29.0	37.0
88-89	32.94459611357453	35.0	34.0	35.0	29.0	36.0
90-91	32.871617566699875	35.0	34.0	35.0	29.0	36.0
92-93	32.7792619685508	35.0	34.0	35.0	29.0	36.0
94-95	32.713523685633916	35.0	34.0	35.0	28.5	36.0
96-97	32.65884612429662	35.0	34.0	35.0	27.5	35.0
98-99	32.598672836830175	35.0	34.0	35.0	27.0	35.0
100	31.853577922432553	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2740671130995622
1101	2	0.32226760937432886
1101	3	0.30386726900767513
1101	4	0.19198092132968725
1101	5	0.20113204586904487
1101	6	0.2273942486314553
1101	7	0.25251872682281373
1101	8	0.2326727896835692
1101	9	0.3037352503400257
1101	10-11	0.2659300164848659
1101	12-13	0.2730019295047299
1101	14-15	0.4717822439256665
1101	16-17	0.48173978432096476
1101	18-19	0.40553713510313827
1101	20-21	0.40736440082492464
1101	22-23	0.36194966240920223
1101	24-25	0.3718977329155351
1101	26-27	0.3155231584267
1101	28-29	0.3984330412242656
1101	30-31	0.49355217079455116
1101	32-33	0.46020236605070863
1101	34-35	0.45813894672487976
1101	36-37	0.45515323068421765
1101	38-39	0.48213500908853035
1101	40-41	0.3800195231333561
1101	42-43	0.36457915734251145
1101	44-45	0.46267638562304825
1101	46-47	0.5370963978528636
1101	48-49	0.44519337936588244
1101	50-51	0.42459629354083717
1101	52-53	0.36196644785358245
1101	54-55	0.3869309179106608
1101	56-57	0.49204600249332486
1101	58-59	0.4707505688712672
1101	60-61	0.4079074981175381
1101	62-63	0.4055858129820251
1101	64-65	0.3965912310332129
1101	66-67	0.38272311295190775
1101	68-69	0.3241377573368496
1101	70-71	0.33256163979091724
1101	72-73	0.27505872703149237
1101	74-75	0.3082784638554088
1101	76-77	0.3368726904813073
1101	78-79	0.22466009085809446
1101	80-81	0.2530843302625172
1101	82-83	0.2392335493759319
1101	84-85	0.2621281967833937
1101	86-87	0.27444391142115876
1101	88-89	0.39221064645958137
1101	90-91	0.3041785556078551
1101	92-93	0.29453780813648933
1101	94-95	0.3294385424432562
1101	96-97	0.265530955430215
1101	98-99	0.2751100450379269
1101	100	0.3278144659288742
1102	1	0.21919538081759526
1102	2	0.2727884054994334
1102	3	0.21988000330941304
1102	4	0.1947009127270718
1102	5	0.15764529255766746
1102	6	0.1497387868810378
1102	7	0.20487566920176903
1102	8	0.16510250425546502
1102	9	0.2572077438087348
1102	10-11	0.2735683940818987
1102	12-13	0.25580378804119164
1102	14-15	0.39449915853433026
1102	16-17	0.46216926776073564
1102	18-19	0.3679655683491845
1102	20-21	0.39732928974235193
1102	22-23	0.33279137987702256
1102	24-25	0.4180432517093422
1102	26-27	0.3591375278907236
1102	28-29	0.35435594844416585
1102	30-31	0.3541879872891869
1102	32-33	0.3872390357832671
1102	34-35	0.34020090700653327
1102	36-37	0.3084810714734232
1102	38-39	0.3541462218660385
1102	40-41	0.3330854696409489
1102	42-43	0.2979749445519886
1102	44-45	0.3883051175276222
1102	46-47	0.4430505234036346
1102	48-49	0.45058547741273003
1102	50-51	0.375955488769506
1102	52-53	0.39146878904190885
1102	54-55	0.3646961846384613
1102	56-57	0.3385743238399783
1102	58-59	0.37409757216685335
1102	60-61	0.2732236542249211
1102	62-63	0.24447317457028817
1102	64-65	0.2945636089071684
1102	66-67	0.3140852759447057
1102	68-69	0.29035921347961846
1102	70-71	0.28473705622612755
1102	72-73	0.257147363909084
1102	74-75	0.27455248132722687
1102	76-77	0.281058268467433
1102	78-79	0.25533493413954744
1102	80-81	0.21380540972532458
1102	82-83	0.1806795115383153
1102	84-85	0.1828378826026409
1102	86-87	0.24162214442058882
1102	88-89	0.24412621712785665
1102	90-91	0.24254534772740755
1102	92-93	0.21969750477499161
1102	94-95	0.23642137238996952
1102	96-97	0.23251767812863733
1102	98-99	0.2702361867764367
1102	100	0.2634594878287544
1103	1	0.2736728842391969
1103	2	0.2704092670683238
1103	3	0.2687750501956998
1103	4	0.19751629631926448
1103	5	0.1914113765818044
1103	6	0.23094512311252657
1103	7	0.20007149722294315
1103	8	0.23761855989709346
1103	9	0.2607159021085579
1103	10-11	0.2832784319558357
1103	12-13	0.2542774936016343
1103	14-15	0.40692715352484043
1103	16-17	0.4673090377150899
1103	18-19	0.3787848187257268
1103	20-21	0.345105961206265
1103	22-23	0.336474905832965
1103	24-25	0.37716857774582735
1103	26-27	0.37370737550766364
1103	28-29	0.3847405285867822
1103	30-31	0.45514452203718037
1103	32-33	0.4019701604503467
1103	34-35	0.41707498578283975
1103	36-37	0.4028274211449343
1103	38-39	0.35840424195714604
1103	40-41	0.34648716576343475
1103	42-43	0.36434073249626664
1103	44-45	0.3773567166825771
1103	46-47	0.38616417050175755
1103	48-49	0.4922599341715568
1103	50-51	0.35522997876605444
1103	52-53	0.3545913381822956
1103	54-55	0.42088485125005803
1103	56-57	0.3409217382781975
1103	58-59	0.3115400321708677
1103	60-61	0.2967549662084821
1103	62-63	0.30273477061563625
1103	64-65	0.3024888903690339
1103	66-67	0.29598247056141247
1103	68-69	0.27096876826317384
1103	70-71	0.2562557546212929
1103	72-73	0.280820467647672
1103	74-75	0.21640586462665823
1103	76-77	0.17952103971763478
1103	78-79	0.2580702830897792
1103	80-81	0.24327852895552127
1103	82-83	0.24130591385555533
1103	84-85	0.2718661960247388
1103	86-87	0.21126977137735992
1103	88-89	0.2509046842985967
1103	90-91	0.18126425559754722
1103	92-93	0.2188596370846554
1103	94-95	0.2547677439623115
1103	96-97	0.2524351120086692
1103	98-99	0.2684829933217614
1103	100	0.32072016774316836
1104	1	0.2287210729177076
1104	2	0.19503513936027872
1104	3	0.19880427359301223
1104	4	0.1362196196202703
1104	5	0.1696021834095376
1104	6	0.1853295588406212
1104	7	0.14937603894536267
1104	8	0.15892087094423601
1104	9	0.18544321511895134
1104	10-11	0.20519214274558806
1104	12-13	0.2195069779689689
1104	14-15	0.3423019149822437
1104	16-17	0.4384351720537225
1104	18-19	0.3714268713225337
1104	20-21	0.3006854124803553
1104	22-23	0.28382821900776634
1104	24-25	0.2562961191917381
1104	26-27	0.23151198098771886
1104	28-29	0.25518919914952676
1104	30-31	0.27960495615140957
1104	32-33	0.28776753118651754
1104	34-35	0.23784217152697096
1104	36-37	0.21894274883239007
1104	38-39	0.21725175293205723
1104	40-41	0.18727123822353064
1104	42-43	0.23641222493466785
1104	44-45	0.1706582921314279
1104	46-47	0.2213961750688327
1104	48-49	0.3257526233216339
1104	50-51	0.23050932981896466
1104	52-53	0.2535715374949348
1104	54-55	0.24424099496787477
1104	56-57	0.22045279743105084
1104	58-59	0.1655390544007247
1104	60-61	0.2001529139117153
1104	62-63	0.16455386191193355
1104	64-65	0.1824607676137262
1104	66-67	0.17299861121865945
1104	68-69	0.17917384771835287
1104	70-71	0.227854369006387
1104	72-73	0.2475160459396193
1104	74-75	0.19079846153609026
1104	76-77	0.13891977486209584
1104	78-79	0.19308211843557643
1104	80-81	0.2971500563266858
1104	82-83	0.3079421585119988
1104	84-85	0.17634011440669894
1104	86-87	0.23476511033452852
1104	88-89	0.29933117189892045
1104	90-91	0.18704293225847124
1104	92-93	0.17450159584370084
1104	94-95	0.1735281434856617
1104	96-97	0.17631487454962524
1104	98-99	0.1409152942712666
1104	100	0.15206446187880118
1105	1	0.13341830142537248
1105	2	0.11513875247394623
1105	3	0.09834971587131491
1105	4	0.10491240530221546
1105	5	0.07685116462235442
1105	6	0.1071435014003157
1105	7	0.09164099368148015
1105	8	0.11023721652695428
1105	9	0.09713621051295718
1105	10-11	0.12097563768762143
1105	12-13	0.14218930676827313
1105	14-15	0.269118616976435
1105	16-17	0.31197406448183074
1105	18-19	0.24597376821525785
1105	20-21	0.20332481344166098
1105	22-23	0.216216304688146
1105	24-25	0.1901876695779734
1105	26-27	0.1505020650978608
1105	28-29	0.19966912930823355
1105	30-31	0.22435065364954454
1105	32-33	0.17416108027487098
1105	34-35	0.11436283782207823
1105	36-37	0.124995380867432
1105	38-39	0.11377285994439035
1105	40-41	0.15949936831236755
1105	42-43	0.17354183367928755
1105	44-45	0.1790641065285712
1105	46-47	0.17925469543256156
1105	48-49	0.29645701076059794
1105	50-51	0.2598646125204951
1105	52-53	0.15026296438131226
1105	54-55	0.19770724828470776
1105	56-57	0.22748327698833037
1105	58-59	0.13541385357108737
1105	60-61	0.13384411127850626
1105	62-63	0.11967216106038592
1105	64-65	0.12818636766383662
1105	66-67	0.12045573475640481
1105	68-69	0.09136477929303766
1105	70-71	0.06776383875672565
1105	72-73	0.09729144109336829
1105	74-75	0.12380986497036872
1105	76-77	0.0746959360714996
1105	78-79	0.04138990048654989
1105	80-81	0.08881431101567472
1105	82-83	0.11491281937241382
1105	84-85	0.1157954473450431
1105	86-87	0.10308011717678767
1105	88-89	0.14416726594599538
1105	90-91	0.14966875021601567
1105	92-93	0.15784050416733209
1105	94-95	0.14183062207435881
1105	96-97	0.1489786709422276
1105	98-99	0.10343803886994607
1105	100	0.11313303772585925
1106	1	0.13039435628603258
1106	2	0.10647609744368225
1106	3	0.16120567752329862
1106	4	0.07987628915538636
1106	5	0.06854586077480462
1106	6	0.08952269029655469
1106	7	0.08453051148110546
1106	8	0.11043180909090466
1106	9	0.18123823108290793
1106	10-11	0.15102869970066024
1106	12-13	0.15584357567469453
1106	14-15	0.25125124427668055
1106	16-17	0.32458487445997264
1106	18-19	0.23960693256766774
1106	20-21	0.19163965815096873
1106	22-23	0.1913649932158279
1106	24-25	0.18721902601398455
1106	26-27	0.2113140357798713
1106	28-29	0.197748092873276
1106	30-31	0.21729987757478142
1106	32-33	0.23482131536599127
1106	34-35	0.19670432003946559
1106	36-37	0.1809492724507109
1106	38-39	0.2366981012100311
1106	40-41	0.19321328741359167
1106	42-43	0.22010930241688698
1106	44-45	0.24896758162550015
1106	46-47	0.2724423942927672
1106	48-49	0.32737436671607156
1106	50-51	0.2673165988444808
1106	52-53	0.19100750116594867
1106	54-55	0.24034739629623658
1106	56-57	0.1871450774426222
1106	58-59	0.20956949080113674
1106	60-61	0.2568920740341838
1106	62-63	0.2090870480207414
1106	64-65	0.24987712302556275
1106	66-67	0.24611680764425614
1106	68-69	0.2657343984299487
1106	70-71	0.15214476756684547
1106	72-73	0.24432828573882404
1106	74-75	0.2669244459659694
1106	76-77	0.22725792778582132
1106	78-79	0.26040164059384807
1106	80-81	0.22858278548222444
1106	82-83	0.2179058161375238
1106	84-85	0.22065478488669754
1106	86-87	0.18658465700964655
1106	88-89	0.23721933262389427
1106	90-91	0.1730724095350027
1106	92-93	0.14638879003989302
1106	94-95	0.20633197455094177
1106	96-97	0.16699241919818064
1106	98-99	0.1318373903523522
1106	100	0.13789220321040574
1107	1	0.019321534633370163
1107	2	0.03476849749485922
1107	3	0.0417967437458131
1107	4	0.031981598662909505
1107	5	-0.001430886577821866
1107	6	0.04974622494207637
1107	7	0.07475563688090148
1107	8	0.042912510373049884
1107	9	0.0695342236676666
1107	10-11	0.04705106628303213
1107	12-13	0.08666572541076079
1107	14-15	0.11701718459217858
1107	16-17	0.19253613423854432
1107	18-19	0.10244606865604311
1107	20-21	0.1223133360299542
1107	22-23	0.14217349692121672
1107	24-25	0.12282590052114273
1107	26-27	0.08820600799772649
1107	28-29	0.11448231631189998
1107	30-31	0.11419046892347495
1107	32-33	0.10446934327231361
1107	34-35	0.06764677067733516
1107	36-37	0.06567612489894259
1107	38-39	0.05131849293087498
1107	40-41	0.06415396714578492
1107	42-43	0.10586422026566567
1107	44-45	0.10465947359728034
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2216	62-63	-0.38629688683182195
2216	64-65	-0.28587845076781093
2216	66-67	-0.3057907182218784
2216	68-69	-0.25013252398264996
2216	70-71	-0.23906458746608905
2216	72-73	-0.2308074696455975
2216	74-75	-0.24004642133819232
2216	76-77	-0.2208497463794643
2216	78-79	-0.2626742663363757
2216	80-81	-0.3044730966109839
2216	82-83	-0.30103721055652244
2216	84-85	-0.2844191849448734
2216	86-87	-0.23958136481022052
2216	88-89	-0.12062000633321901
2216	90-91	-0.1928777990825168
2216	92-93	-0.1721993167511755
2216	94-95	-0.24174322304448737
2216	96-97	-0.2645384495572074
2216	98-99	-0.31212218693328
2216	100	-0.3922202052579955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	22.0
8	106.0
9	311.0
10	343.0
11	201.0
12	208.0
13	274.0
14	456.0
15	945.0
16	1644.0
17	2985.0
18	5120.0
19	7565.0
20	11093.0
21	16695.0
22	24024.0
23	28159.0
24	25892.0
25	22319.0
26	20352.0
27	22171.0
28	27289.0
29	33005.0
30	39773.0
31	50141.0
32	63682.0
33	84293.0
34	123168.0
35	194260.0
36	358084.0
37	797748.0
38	947041.0
39	330929.0
40	675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.060652362803374	20.308396196607358	13.664708771895178	23.96624266869409
2	15.463343349199501	21.036342845012033	39.68637317648598	23.813940629302486
3	17.463028781224864	26.96250841184128	31.28004697363472	24.294415833299134
4	12.36371606697157	18.1010624258131	39.127832222234225	30.4073892849811
5	12.660040914687015	38.42521653002724	35.17848492235158	13.736257632934162
6	28.792673692473556	39.049263549219425	18.089282507419433	14.068780250887587
7	24.969692743506346	33.97596956222715	23.408649192696142	17.645688501570362
8	25.53081461615409	34.38408102285679	20.959054749793427	19.12604961119569
9	26.564028765435566	14.429154454541893	21.25229059297933	37.75452618704321
10-11	24.541588140841696	26.091277555005103	28.533831825760913	20.833302478392284
12-13	25.451369079594304	24.347086507662976	28.364522012370973	21.83702240037174
14-15	22.92404517471222	25.126192832552576	26.740631091439376	25.20913090129583
16-17	21.43424213644944	27.83949291661807	27.671256039094516	23.05500890783797
18-19	21.23459296612034	26.857228898376444	30.352955950546917	21.5552221849563
20-21	22.62946870941122	25.85955454954564	29.928942492926108	21.582034248117036
22-23	22.735224884625698	25.700846628466206	29.580190887119397	21.983737599788704
24-25	22.254026799976426	25.93381678897047	30.063101420468485	21.749054990584618
26-27	22.01384648470657	26.209492413838216	29.767658849966693	22.00900225148852
28-29	21.65249782279856	26.416098871557843	29.86976902766068	22.061634277982918
30-31	22.229538282864343	25.981227207805574	29.85797140174522	21.93126310758487
32-33	22.382762318497598	26.364163562785762	29.210750054883267	22.04232406383337
34-35	22.422448721732408	26.18491972384181	29.729492896953484	21.663138657472295
36-37	22.437096031684323	25.96744124910675	29.601536205804923	21.993926513404002
38-39	22.413470986366498	25.889395718941095	29.782219753124895	21.91491354156751
40-41	22.284436269915577	25.688904357549447	29.839586184531967	22.187073188003005
42-43	22.325579386190505	25.84065032322084	29.34089546565183	22.49287482493683
44-45	22.25201961639785	26.39669949848224	29.078434119252243	22.272846765867666
46-47	22.578790381777324	26.121414772662405	28.501687610479937	22.798107235080327
48-49	23.03466956950221	26.000156126146223	28.35175220744166	22.613422096909904
50-51	22.705588722892784	26.406036088545015	27.77517739271509	23.113197795847114
52-53	22.38281756818154	26.749206986877194	27.901874181473612	22.966101263467646
54-55	22.4270926798323	26.64866070712294	27.78989522870356	23.134351384341205
56-57	22.84219493749859	26.40901619794339	27.579531047369503	23.169257817188512
58-59	22.18130822954416	26.56529191331541	28.21351488531894	23.03988497182149
60-61	22.430754913988235	26.8989173551475	27.819356781587633	22.850970949276633
62-63	22.56638567538418	26.45084421576095	27.713168278660085	23.269601830194787
64-65	22.004615297746565	26.49371590710286	27.88011025124955	23.621558543901024
66-67	22.44187060071353	26.30482692122264	27.76571593867515	23.48758653938868
68-69	22.133124584026174	26.18480608252859	27.923727100804747	23.75834223264049
70-71	22.169778863637976	26.026944774690353	27.93943627769373	23.863840083977944
72-73	22.405682791544994	26.047141016368435	27.998097425631123	23.549078766455448
74-75	22.37375342499568	26.386058724790797	27.69726123768853	23.54292661252499
76-77	22.2920704979027	26.330660564533698	27.49372538056562	23.88354355699798
78-79	22.0893710211043	26.344140681949845	27.573865814647196	23.99262248229866
80-81	21.927137715771	26.461244529358186	27.753741511711432	23.857876243159385
82-83	22.264180110264036	25.87466691844485	28.183219848019775	23.677933123271337
84-85	22.44788297510777	26.26304343030126	27.387327456149833	23.901746138441133
86-87	21.70629340421741	26.54550447435145	27.69080885444107	24.05739326699008
88-89	22.09402855253654	25.866105641731664	27.575160299083024	24.464705506648777
90-91	21.89482911458997	25.850400481583772	27.748287319888192	24.506483083938065
92-93	22.0916817830212	25.869932190075133	27.567941829778793	24.470444197124873
94-95	21.319108982254683	25.929151414021984	27.92622451070174	24.82551509302159
96-97	21.477392956837775	25.893986934114256	27.7601321090455	24.86848800000247
98-99	21.362290525546687	26.110516981346315	27.575989642600607	24.951202850506395
100	21.752237687891828	25.757827932014184	27.282670150585194	25.207264229508798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	143.0
2	234.0
3	402.5
4	273.0
5	236.0
6	483.5
7	1271.5
8	2433.5
9	3005.0
10	2303.5
11	1509.0
12	1581.5
13	1843.5
14	2175.0
15	2608.5
16	3093.5
17	3669.5
18	4295.0
19	4875.5
20	5484.5
21	6229.0
22	7229.0
23	8654.5
24	10407.0
25	12249.5
26	15150.0
27	19008.0
28	22482.5
29	27372.0
30	34216.0
31	42003.0
32	50190.0
33	56254.0
34	62915.0
35	70111.0
36	77923.0
37	89238.5
38	95137.5
39	95101.5
40	93742.0
41	92093.5
42	93707.5
43	99040.5
44	105727.0
45	116734.5
46	127037.5
47	132749.5
48	135439.5
49	130680.5
50	124689.0
51	124023.5
52	123944.0
53	122328.0
54	118721.0
55	108466.0
56	98583.0
57	86499.5
58	73472.5
59	65850.0
60	54779.0
61	41996.0
62	32591.0
63	25601.5
64	18979.0
65	12845.5
66	9074.0
67	6827.5
68	5107.5
69	3884.5
70	3031.0
71	2358.0
72	1882.0
73	1556.0
74	1127.0
75	732.0
76	502.0
77	299.0
78	152.0
79	99.5
80	60.5
81	24.5
82	13.0
83	6.5
84	6.5
85	4.0
86	2.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00237582972767746
2	0.0035483171257520508
3	1.851295891696722E-4
4	0.05208312441973444
5	0.002560959316847132
6	0.004905934112996313
7	0.0
8	3.394042468110657E-4
9	0.0
10-11	3.0854931528278694E-5
12-13	0.0
14-15	1.5427465764139347E-5
16-17	2.1598452069795089E-4
18-19	6.170986305655739E-5
20-21	2.0055705493381154E-4
22-23	0.0
24-25	0.0
26-27	1.851295891696722E-4
28-29	1.5427465764139347E-5
30-31	1.851295891696722E-4
32-33	1.0799226034897544E-4
34-35	1.3884719187725415E-4
36-37	3.0854931528278694E-5
38-39	6.942359593862707E-4
40-41	3.54831712575205E-4
42-43	0.0
44-45	3.394042468110657E-4
46-47	0.0
48-49	1.2341972611311478E-4
50-51	0.0
52-53	3.54831712575205E-4
54-55	2.776943837545083E-4
56-57	1.851295891696722E-4
58-59	8.639380827918035E-4
60-61	6.479535620938526E-4
62-63	2.0055705493381154E-4
64-65	3.702591783393444E-4
66-67	0.0010953500692538938
68-69	0.0025918142483754105
70-71	0.0037643016464500014
72-73	0.0031163480843561485
74-75	0.002869508632129919
76-77	0.004288835482430739
78-79	0.0014038993845366808
80-81	0.0011416324665463118
82-83	7.713732882069675E-4
84-85	6.633810278579921E-4
86-87	1.5427465764139347E-5
88-89	0.0
90-91	0.0
92-93	6.170986305655739E-5
94-95	1.542746576413935E-4
96-97	4.3196904139590177E-4
98-99	1.542746576413935E-4
100	0.0763351006009615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3240973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.57454346454351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.67191035544955	31.2431115360494
2	16.292001793717922	15.501690949193081
3	7.019814063881675	10.018933478854574
4	3.6740791431724977	6.99170551556131
5	2.0974475066349365	4.989255378450109
6	1.345818198275916	3.8416011821550717
7	0.8994043199261744	2.9952124937368185
8	0.623555868379746	2.3732308610242714
9	0.4526526785674186	1.9381270077762924
>10	1.8504157946531734	14.177094740735729
>50	0.04801406899340623	1.5398718040518022
>100	0.022475146185242392	1.82071684490468
>500	0.0013151248158718065	0.466902228003173
>1k	7.671561425918867E-4	0.6261338756102849
>5k	1.0959373465598381E-4	0.30542861548214245
>10k+	2.1918746931196763E-4	1.1709834884113073
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11530	0.3557573605210534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10929	0.3372135466725579	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5858	0.18074818889265662	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	3772	0.11638480172466725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.08549315282787E-5	0.0
22-23	0.0	0.0	0.0	3.08549315282787E-4	0.0
24-25	0.0	0.0	0.0	8.176556854993856E-4	0.0
26-27	0.0	0.0	0.0	0.0018050134944043038	0.0
28-29	0.0	0.0	0.0	0.005430467948977051	0.0
30-31	0.0	0.0	0.0	0.017726158162996112	0.0
32-33	0.0	0.0	0.0	0.046236114895125635	0.0
34-35	0.0	0.0	0.0	0.09188598609121397	0.0
36-37	0.0	0.0	0.0	0.16445678504572547	0.0
38-39	0.0	0.0	0.0	0.26942526210492956	0.0
40-41	0.0	0.0	0.0	0.3825394410875993	0.0
42-43	0.0	0.0	0.0	0.5025034148695469	0.0
44-45	0.0	0.0	0.0	0.6575031634018549	0.0
46-47	0.0	0.0	0.0	0.8444069111344031	0.0
48-49	0.0	0.0	0.0	1.042541853943245	0.0
50-51	0.0	0.0	0.0	1.2514143129239275	0.0
52-53	0.0	0.0	0.0	1.464915011633852	0.0
54-55	0.0	0.0	0.0	1.6973760657679038	0.0
56-57	0.0	0.0	0.0	1.9766594784961184	0.0
58-59	0.0	0.0	0.0	2.298029017828905	0.0
60-61	0.0	0.0	0.0	2.632450193198154	0.0
62-63	0.0	0.0	0.0	2.9710984941867764	0.0
64-65	0.0	0.0	0.0	3.3314686669713076	0.0
66-67	0.0	0.0	0.0	3.737735550404153	0.0
68-69	0.0	0.0	0.0	4.189606022635795	0.0
70-71	0.0	0.0	0.0	4.676419704823212	0.0
72-73	0.0	0.0	0.0	5.176099893457922	0.0
74-75	0.0	0.0	0.0	5.683432105111644	0.0
76-77	0.0	0.0	0.0	6.237308980975775	0.0
78-79	0.0	0.0	0.0	6.844441468657715	0.0
80-81	0.0	0.0	0.0	7.497702078974431	0.0
82-83	0.0	0.0	0.0	8.168997396769427	0.0
84-85	0.0	0.0	0.0	8.836127298808105	0.0
86-87	0.0	0.0	0.0	9.53761416710352	0.0
88	0.0	0.0	0.0	10.089007220979626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6660	0.0	64.328026	1
GTATCAA	12830	0.0	43.802338	1
ATCAACG	16275	0.0	33.864338	3
CAACGCA	16380	0.0	33.65986	5
TATCAAC	16730	0.0	33.45136	2
AACGCAG	17755	0.0	31.739975	6
TCAACGC	18090	0.0	30.674635	4
GTGGTAT	1575	0.0	30.157606	1
ACGCAGA	19405	0.0	28.409632	7
CGCAGAG	19665	0.0	27.986217	8
GCAGAGT	21290	0.0	25.628967	9
GTACATG	12240	0.0	25.242943	1
TACATGG	12130	0.0	24.851921	2
ACATGGG	12615	0.0	23.522205	3
CAGAGTA	20525	0.0	23.081036	10-11
GAGTACT	14405	0.0	22.74054	12-13
AGAGTAC	20230	0.0	21.756538	10-11
GTGATCG	1155	0.0	21.566275	8
TCTAACG	885	0.0	21.2559	2
CTATTGA	2620	0.0	20.808043	9
>>END_MODULE