FastQCFastQC Report
Mon 16 Jan 2017
ERR1146593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4244529
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.036.7816051
GTATCAA67300.035.7122541
CAACGCA72200.031.058285
ATCAACG74350.030.288763
AACGCAG82050.027.893186
TATCAAC86100.027.4111672
TCAACGC86700.026.136954
GTACATG150650.025.6633951
ACGCAGA88400.025.1984467
TACATGG152800.024.8300272
GTACTAG17050.023.999731
CGCAGAG92950.023.9641088
ACATGGG156150.023.604843
CATGGGG116800.021.896924
GTGATCG11200.021.3985568
TGATCGC11500.020.8388589
GCTATTG28650.020.3389918
GCAGAGT107150.020.304419
GGCTATT32650.019.143277
GGGCTAT33250.019.0805026
Produced by FastQC (version 0.11.5)