##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146593_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4244529
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10037627260881	31.0	31.0	34.0	28.0	34.0
2	31.378884912790088	33.0	31.0	34.0	28.0	34.0
3	31.47318489283499	33.0	31.0	34.0	28.0	34.0
4	34.96306798704874	37.0	35.0	37.0	32.0	37.0
5	34.94410734383014	37.0	35.0	37.0	32.0	37.0
6	35.06132977298541	37.0	35.0	37.0	32.0	37.0
7	34.97064291467911	37.0	35.0	37.0	32.0	37.0
8	34.94248878968668	37.0	35.0	37.0	32.0	37.0
9	36.53971076649494	39.0	37.0	39.0	32.0	39.0
10-11	36.52464172114267	39.0	37.0	39.0	32.0	39.0
12-13	36.46982091534773	39.0	37.0	39.0	32.0	39.0
14-15	37.81034244317803	40.0	38.0	41.0	32.0	41.0
16-17	37.83567505369854	40.0	38.0	41.0	32.0	41.0
18-19	37.85786797545735	40.0	38.0	41.0	32.0	41.0
20-21	37.95587896796087	40.0	38.0	41.0	32.0	41.0
22-23	37.970169010507405	40.0	38.0	41.0	32.0	41.0
24-25	37.99171368601793	40.0	38.0	41.0	32.0	41.0
26-27	38.054954036125096	40.0	38.0	41.0	32.0	41.0
28-29	38.03863549995771	40.0	38.0	41.0	32.0	41.0
30-31	38.005472927620474	40.0	38.0	41.0	32.0	41.0
32-33	37.9721211705704	40.0	38.0	41.0	32.0	41.0
34-35	37.925992730877795	40.0	38.0	41.0	31.5	41.0
36-37	37.852598250595065	40.0	38.0	41.0	31.0	41.0
38-39	37.79188621399453	40.0	38.0	41.0	31.0	41.0
40-41	37.735332118121946	40.0	38.0	41.0	31.0	41.0
42-43	37.58106835882144	40.0	37.0	41.0	30.5	41.0
44-45	37.461949252791065	40.0	37.0	41.0	30.0	41.0
46-47	37.34755917558815	40.0	37.0	41.0	30.0	41.0
48-49	37.234131042572685	40.0	36.0	41.0	30.0	41.0
50-51	37.102258578042466	40.0	36.0	41.0	30.0	41.0
52-53	36.96428531881865	40.0	35.5	41.0	30.0	41.0
54-55	36.83789614819453	39.0	35.0	41.0	30.0	41.0
56-57	36.64471970859428	39.0	35.0	41.0	30.0	41.0
58-59	36.44761468233578	39.0	35.0	41.0	30.0	41.0
60-61	36.22987014578061	38.5	35.0	40.5	29.5	41.0
62-63	35.99042060968367	38.0	35.0	40.0	29.0	41.0
64-65	35.690379191660604	37.0	35.0	40.0	29.0	41.0
66-67	35.44842454840101	37.0	34.0	40.0	29.0	41.0
68-69	35.19459567834264	36.0	34.0	39.0	29.0	41.0
70-71	34.91894742620441	36.0	34.0	39.0	28.5	41.0
72-73	34.58825148797428	35.0	34.0	39.0	28.0	40.5
74-75	34.27240937687079	35.0	34.0	37.5	28.0	40.0
76-77	34.01079742887845	35.0	34.0	37.0	28.0	39.0
78-79	33.71637394867605	35.0	34.0	37.0	27.0	39.0
80-81	33.4921787552871	35.0	33.5	36.0	27.0	39.0
82-83	33.23855096761031	35.0	33.0	36.0	27.0	37.0
84-85	33.05184026307748	35.0	33.0	35.5	27.0	37.0
86-87	32.862975138113086	35.0	33.0	35.0	26.5	37.0
88-89	32.70257901406728	35.0	33.0	35.0	26.0	36.0
90-91	32.573112941388786	35.0	33.0	35.0	26.0	36.0
92-93	32.43394343636243	35.0	33.0	35.0	26.0	36.0
94-95	32.323007923847385	35.0	33.0	35.0	25.5	36.0
96-97	32.2411285209737	35.0	33.0	35.0	25.0	35.0
98-99	32.144949533858764	35.0	33.0	35.0	25.0	35.0
100	31.007789556862495	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27782535487832405
1101	2	0.3779142929380832
1101	3	0.420217732513013
1101	4	0.417557011760465
1101	5	0.3413075617364285
1101	6	0.3678081872221881
1101	7	0.3546108588266321
1101	8	0.3945367016535499
1101	9	0.499247406556087
1101	10-11	0.535800987494099
1101	12-13	0.4907784516724334
1101	14-15	0.592176720534404
1101	16-17	0.5464439641618455
1101	18-19	0.5711279726122953
1101	20-21	0.5694387665820102
1101	22-23	0.5375933533812471
1101	24-25	0.5504137596580989
1101	26-27	0.6006360469819967
1101	28-29	0.5938497980656976
1101	30-31	0.6228220246007439
1101	32-33	0.633423967719132
1101	34-35	0.5826552787258947
1101	36-37	0.6397927199390381
1101	38-39	0.6667840409499277
1101	40-41	0.6276975364493254
1101	42-43	0.6218733824233453
1101	44-45	0.5866709004801791
1101	46-47	0.512132566802677
1101	48-49	0.5081738856924076
1101	50-51	0.5070527806103229
1101	52-53	0.4246535615278333
1101	54-55	0.39843207663532354
1101	56-57	0.47482162148709506
1101	58-59	0.42731487797031065
1101	60-61	0.47131434380866466
1101	62-63	0.4363683630310504
1101	64-65	0.48689745081706093
1101	66-67	0.4217563769170667
1101	68-69	0.4837193642756361
1101	70-71	0.4465004936905643
1101	72-73	0.45389720294268443
1101	74-75	0.4356041082499331
1101	76-77	0.46157402728557173
1101	78-79	0.4739249972262556
1101	80-81	0.36973533954317617
1101	82-83	0.4335106766781678
1101	84-85	0.3817530742564017
1101	86-87	0.34273141393647677
1101	88-89	0.3460084959057781
1101	90-91	0.4082599941865652
1101	92-93	0.3972045173644858
1101	94-95	0.34149573024092916
1101	96-97	0.36640039330552554
1101	98-99	0.45166670083940375
1101	100	0.32741506406602383
1102	1	0.2696133229887998
1102	2	0.33106546032828277
1102	3	0.29059182368315106
1102	4	0.283777421517982
1102	5	0.31990619698139966
1102	6	0.41296721279437776
1102	7	0.4117374917412917
1102	8	0.4505512396212481
1102	9	0.5101830565194305
1102	10-11	0.5838631710690336
1102	12-13	0.5123280096910747
1102	14-15	0.5600059556857033
1102	16-17	0.5306012370350572
1102	18-19	0.5213102930268505
1102	20-21	0.5640705922190818
1102	22-23	0.5538806437483501
1102	24-25	0.6507414431991165
1102	26-27	0.5522545213229151
1102	28-29	0.5479104656139597
1102	30-31	0.6082419855084353
1102	32-33	0.5770755066682725
1102	34-35	0.5499576118512408
1102	36-37	0.5467354766912251
1102	38-39	0.5761418646241125
1102	40-41	0.5048285717995569
1102	42-43	0.6272425663673715
1102	44-45	0.5217186404253269
1102	46-47	0.49708514131720705
1102	48-49	0.5340533715011802
1102	50-51	0.5522234908423371
1102	52-53	0.5157332505768579
1102	54-55	0.5195578754042955
1102	56-57	0.5283873129425629
1102	58-59	0.5066358767604697
1102	60-61	0.4673997510587782
1102	62-63	0.46285747031311075
1102	64-65	0.5370873017135196
1102	66-67	0.4936245818984375
1102	68-69	0.44107588376965623
1102	70-71	0.4347088182659178
1102	72-73	0.47381044517098303
1102	74-75	0.4877322886372468
1102	76-77	0.4521436461769852
1102	78-79	0.3867272302210196
1102	80-81	0.337409744676485
1102	82-83	0.36130495715303823
1102	84-85	0.4094731587964091
1102	86-87	0.3415887998496743
1102	88-89	0.2795456211815548
1102	90-91	0.3147835927307838
1102	92-93	0.33633675759182324
1102	94-95	0.3338336717878363
1102	96-97	0.330697071121719
1102	98-99	0.37039052413953755
1102	100	0.4541865395936142
1103	1	0.20225586617196356
1103	2	0.2584005863453207
1103	3	0.29730340274456424
1103	4	0.3523442626879145
1103	5	0.2746044979446083
1103	6	0.3468621165868484
1103	7	0.31146344286934635
1103	8	0.3200171813896233
1103	9	0.4228745649468948
1103	10-11	0.3935155238535373
1103	12-13	0.4466447007601104
1103	14-15	0.48354085809378233
1103	16-17	0.5008557539810354
1103	18-19	0.43342636202360296
1103	20-21	0.4251331861732126
1103	22-23	0.4613529145830242
1103	24-25	0.48940431884635416
1103	26-27	0.45502819024022045
1103	28-29	0.4584167006320854
1103	30-31	0.4417671670966641
1103	32-33	0.4626110170037876
1103	34-35	0.4580645198029032
1103	36-37	0.4183030266082426
1103	38-39	0.37405188835434444
1103	40-41	0.35104842183410057
1103	42-43	0.41288906860270913
1103	44-45	0.3386444687640804
1103	46-47	0.2801601706978545
1103	48-49	0.311266912031428
1103	50-51	0.3056333467906285
1103	52-53	0.30119648676719635
1103	54-55	0.22605318879539738
1103	56-57	0.234863636125219
1103	58-59	0.27081682244877214
1103	60-61	0.2644046852024786
1103	62-63	0.29909140500303977
1103	64-65	0.20314331229506166
1103	66-67	0.27388608098128486
1103	68-69	0.17764720313771676
1103	70-71	0.23485670714761397
1103	72-73	0.22960509209906377
1103	74-75	0.1939639059800342
1103	76-77	0.15756912766604358
1103	78-79	0.18139830987907857
1103	80-81	0.11005472558846208
1103	82-83	0.2101789672018981
1103	84-85	0.20340240146027355
1103	86-87	0.16703197744423903
1103	88-89	0.20244462246512285
1103	90-91	0.21743365976832507
1103	92-93	0.20881767333518297
1103	94-95	0.221623659945287
1103	96-97	0.27423779699309847
1103	98-99	0.2201896823707088
1103	100	0.309500472417632
1104	1	0.10951316660744581
1104	2	0.16265021510484345
1104	3	0.1989969447958373
1104	4	0.19067959428473102
1104	5	0.1038053656593334
1104	6	0.20249966569164712
1104	7	0.2582115592534464
1104	8	0.26332419137202123
1104	9	0.34438947387986474
1104	10-11	0.31370549407034787
1104	12-13	0.33993465183087324
1104	14-15	0.4396805378401538
1104	16-17	0.3891155840449798
1104	18-19	0.36359394288930247
1104	20-21	0.38740398581497715
1104	22-23	0.4326273632050217
1104	24-25	0.39476824798757804
1104	26-27	0.33309810682494145
1104	28-29	0.3222627183792568
1104	30-31	0.29508627608246485
1104	32-33	0.3101245814872513
1104	34-35	0.29658091960312305
1104	36-37	0.3575506785256266
1104	38-39	0.3469618272010564
1104	40-41	0.30437307720963247
1104	42-43	0.30787878838670224
1104	44-45	0.20242202507223084
1104	46-47	0.2661287744266616
1104	48-49	0.23585365533785563
1104	50-51	0.24181195779284792
1104	52-53	0.2430802514597019
1104	54-55	0.18973908493421732
1104	56-57	0.2618284258715917
1104	58-59	0.18633501590974078
1104	60-61	0.21217360072176206
1104	62-63	0.22763773793915476
1104	64-65	0.2660362020723781
1104	66-67	0.2563022886668378
1104	68-69	0.18816026039863232
1104	70-71	0.1825494900729865
1104	72-73	0.1950989491829631
1104	74-75	0.17446241178379296
1104	76-77	0.13549623339951467
1104	78-79	0.1463213317212535
1104	80-81	0.08662355926665555
1104	82-83	0.13685565152843537
1104	84-85	0.13308379031794715
1104	86-87	0.1086196934531003
1104	88-89	0.12192090027255631
1104	90-91	0.16783670514155347
1104	92-93	0.18688219405850504
1104	94-95	0.16833534558055163
1104	96-97	0.13468597075743105
1104	98-99	0.13238174991022333
1104	100	0.25643909397304654
1105	1	0.1798817610575476
1105	2	0.25343199030901076
1105	3	0.22312740003722809
1105	4	0.27030722337553925
1105	5	0.1575744868867659
1105	6	0.22742137083432823
1105	7	0.19581791733092757
1105	8	0.23682987920113874
1105	9	0.24436208379848523
1105	10-11	0.2663356214738002
1105	12-13	0.2916032015656356
1105	14-15	0.37205090851637834
1105	16-17	0.35589938976259106
1105	18-19	0.3816434817741836
1105	20-21	0.35489073766961354
1105	22-23	0.34991129598026305
1105	24-25	0.3774181712626614
1105	26-27	0.3947685439290112
1105	28-29	0.3273147862013701
1105	30-31	0.42910562600503255
1105	32-33	0.3707536105346847
1105	34-35	0.33779120678089924
1105	36-37	0.38013128011985486
1105	38-39	0.4327542891822631
1105	40-41	0.40046189574226077
1105	42-43	0.3338328260408474
1105	44-45	0.3240520944954213
1105	46-47	0.30742366633133145
1105	48-49	0.3474501288992897
1105	50-51	0.4139531567592485
1105	52-53	0.3508845665330398
1105	54-55	0.29156984175898515
1105	56-57	0.3129115728336629
1105	58-59	0.28824465685708844
1105	60-61	0.31627315781794607
1105	62-63	0.24371844267631104
1105	64-65	0.34530893863571777
1105	66-67	0.3599223903026214
1105	68-69	0.30961122759367043
1105	70-71	0.2719159533614146
1105	72-73	0.2530197237166547
1105	74-75	0.2988846092425419
1105	76-77	0.26035663775075335
1105	78-79	0.2569141188048363
1105	80-81	0.22241405989781526
1105	82-83	0.23158864382772038
1105	84-85	0.2396011844820336
1105	86-87	0.2068517600467885
1105	88-89	0.19140718828511183
1105	90-91	0.11311499576648743
1105	92-93	0.0741186742059412
1105	94-95	0.19006301797590908
1105	96-97	0.17525167916696205
1105	98-99	0.1320593745019707
1105	100	0.1643868284674994
1106	1	0.10288535154828793
1106	2	0.16571207606555305
1106	3	0.19214179786197505
1106	4	0.2343888979405051
1106	5	0.23762005894293736
1106	6	0.2352816908055715
1106	7	0.3066713627930113
1106	8	0.2632960658876442
1106	9	0.3456363342610089
1106	10-11	0.33288991674240265
1106	12-13	0.35868953149413585
1106	14-15	0.3879270343495307
1106	16-17	0.3709031689093649
1106	18-19	0.40427834934656914
1106	20-21	0.4318455807156383
1106	22-23	0.373124639093497
1106	24-25	0.4458714004077251
1106	26-27	0.41822157715997577
1106	28-29	0.3505309280874158
1106	30-31	0.3590887934621989
1106	32-33	0.3131385018966313
1106	34-35	0.3776589220473028
1106	36-37	0.34408253722816795
1106	38-39	0.29223837813203346
1106	40-41	0.299319950903687
1106	42-43	0.3379350101201197
1106	44-45	0.31037785295683307
1106	46-47	0.2858965369694033
1106	48-49	0.22994141594455897
1106	50-51	0.30341474062950624
1106	52-53	0.27553727699184805
1106	54-55	0.2301440231520857
1106	56-57	0.23828004600506603
1106	58-59	0.20073803856285366
1106	60-61	0.23652378698609766
1106	62-63	0.20194416051668185
1106	64-65	0.2538759634729857
1106	66-67	0.2491870825140694
1106	68-69	0.2907015400285857
1106	70-71	0.2453979692909627
1106	72-73	0.26407902762355207
1106	74-75	0.27863839383286404
1106	76-77	0.26158797726778715
1106	78-79	0.2570225344254382
1106	80-81	0.2786131100130689
1106	82-83	0.2061599715974154
1106	84-85	0.2146015681415605
1106	86-87	0.21187018735447793
1106	88-89	0.19193894172859416
1106	90-91	0.20195685634459437
1106	92-93	0.2200139605181235
1106	94-95	0.20051759947728698
1106	96-97	0.1914921945075463
1106	98-99	0.17211909361820688
1106	100	0.22934862200467876
1107	1	0.0658476424924146
1107	2	0.12304313016241863
1107	3	0.12929985218064388
1107	4	0.10658666639556458
1107	5	0.07906018983896956
1107	6	0.09085090313348587
1107	7	0.10162346565794422
1107	8	0.17655832649025882
1107	9	0.2578297909618641
1107	10-11	0.19351364757832812
1107	12-13	0.24785925400470177
1107	14-15	0.22125651998216256
1107	16-17	0.19122195619529947
1107	18-19	0.1630911084751645
1107	20-21	0.24431268755427737
1107	22-23	0.2587743893294743
1107	24-25	0.3164267039839501
1107	26-27	0.18948301659253985
1107	28-29	0.21007176619749401
1107	30-31	0.2542375433667061
1107	32-33	0.2718046374225267
1107	34-35	0.2574408704775166
1107	36-37	0.29558296454756317
1107	38-39	0.28778463753176453
1107	40-41	0.23277314364960233
1107	42-43	0.3065677719796369
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2216	52-53	-0.7226225324372066
2216	54-55	-0.6837169455205938
2216	56-57	-0.5913291824301865
2216	58-59	-0.5553108320377191
2216	60-61	-0.6420628889892086
2216	62-63	-0.6353439812426416
2216	64-65	-0.6715954959830341
2216	66-67	-0.6178733379116679
2216	68-69	-0.7132434477263416
2216	70-71	-0.6185298699274568
2216	72-73	-0.5788634308102871
2216	74-75	-0.5632736875334601
2216	76-77	-0.5691500671585246
2216	78-79	-0.5578094576944963
2216	80-81	-0.5914510134539199
2216	82-83	-0.5887587570763628
2216	84-85	-0.5498816430874527
2216	86-87	-0.5874276898150725
2216	88-89	-0.6023336254913829
2216	90-91	-0.5588620544866743
2216	92-93	-0.530029032734948
2216	94-95	-0.5584864803681171
2216	96-97	-0.6005901695545788
2216	98-99	-0.5933598272256972
2216	100	-0.6468547077233566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1249.0
1	349.0
2	222.0
3	218.0
4	176.0
5	232.0
6	328.0
7	611.0
8	1763.0
9	2732.0
10	3258.0
11	4131.0
12	5814.0
13	7384.0
14	9627.0
15	11078.0
16	12005.0
17	13256.0
18	15197.0
19	16934.0
20	19003.0
21	21173.0
22	23457.0
23	27373.0
24	33099.0
25	40513.0
26	43796.0
27	41173.0
28	43512.0
29	53158.0
30	60954.0
31	70023.0
32	88353.0
33	117293.0
34	174803.0
35	268329.0
36	497519.0
37	1008517.0
38	1142797.0
39	362474.0
40	646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1424131025186	20.117143097018975	13.363279440716708	24.377164359745716
2	15.035201032397019	20.55550277371829	40.178543012259716	24.230753181624973
3	17.89949583335102	26.33720170768709	29.97325195741251	25.790050501549377
4	12.277409728326687	17.529007473751328	39.49189997774097	30.701682820181013
5	13.356842300105992	38.39415128975096	34.96542625623831	13.28358015390474
6	29.932902080521277	38.47726348773354	18.35548078746084	13.234353644284344
7	26.522404910706705	32.75416566844052	23.293976499084145	17.429452921768622
8	25.265976637349773	35.618255672415444	20.905935833593112	18.20983185664167
9	26.28825546339898	15.678141429363945	20.720587968321233	37.31301513891584
10-11	24.527497095985336	26.620568688184687	28.524397436851167	20.327536778978814
12-13	24.949428745219894	24.50078273457282	28.003295972689163	22.546492547518117
14-15	22.652448908616126	25.677825131007324	25.388990079400166	26.28073588097638
16-17	22.362845619607107	28.2936798459839	26.37048276219815	22.972991772210847
18-19	22.273635479651148	28.14591131973505	27.5245548114132	22.0558983892006
20-21	23.56737521935238	27.313228949539347	26.75563832573808	22.3637575053702
22-23	23.60817325063745	27.079141687730257	26.420738715167563	22.89194634646473
24-25	23.188362912040077	27.358552069656728	26.759334655050925	22.693750363252274
26-27	22.9516753910346	27.5085008802957	26.56026886888928	22.979554859780418
28-29	22.807445475564744	27.637825426549455	26.673340994820716	22.88138810306508
30-31	23.197523282304104	27.391739393348473	26.42295550120958	22.987781823137844
32-33	23.161855688456633	27.694612914061217	26.07817705479779	23.065354342684355
34-35	23.381231389762842	27.540527650884773	26.46979239066421	22.60844856868817
36-37	23.35371920645893	27.204110769204647	26.489581416176595	22.952588608159825
38-39	23.2237574275269	27.277011485172892	26.679154300965035	22.82007678633518
40-41	23.137371229799044	27.018692783209552	26.989052764421036	22.85488322257037
42-43	23.0386598518558	27.026937163518323	26.647267981876897	23.28713500274898
44-45	23.01649003738207	27.473900579677952	26.577089291815724	22.93252009112425
46-47	23.116489734774884	27.30987165712732	26.383875101407245	23.18976350669055
48-49	23.247147629937373	27.01395631819203	26.574037227460334	23.16485882441026
50-51	23.03516213372163	27.203683343306107	26.4072841533115	23.353870369660758
52-53	22.87025564667246	27.30023285198641	26.44300314446869	23.386508356872433
54-55	22.95772409408773	27.172534101991886	26.509900943405125	23.35984086051526
56-57	23.14842239654988	27.159132338147025	26.396070223032602	23.2963750422705
58-59	22.849453141627798	27.064451523250106	26.771752145898077	23.314343189224022
60-61	22.905065809836838	27.348192256105648	26.501392839771842	23.24534909428567
62-63	22.987560948029497	27.072993545059653	26.42339611953649	23.51604938737436
64-65	22.660921493295426	27.13205369509279	26.567130775487563	23.639894036124222
66-67	22.969339946084983	27.008827313577697	26.45912336742338	23.562709372913936
68-69	22.78928120907599	27.017479068251543	26.61688515207193	23.576354570600536
70-71	22.863928836363755	26.82391426648701	26.54301138221506	23.76914551493418
72-73	23.024270753667544	26.99546808522179	26.61863785032017	23.361623310790495
74-75	22.953581472681282	27.25085343348525	26.541822662712473	23.25374243112099
76-77	22.895718299715128	27.144850414583843	26.498191829822943	23.46123945587809
78-79	22.853465016818134	27.03920174827467	26.56680344126719	23.540529793640005
80-81	22.819285933780804	27.13250937267629	26.712180481768648	23.336024211774255
82-83	23.207686273695906	26.7956273445913	26.95632014316718	23.040366238545612
84-85	23.22555996438868	26.979771120636286	26.42292567021797	23.37174324475706
86-87	22.744776017855983	27.28707850243782	26.62754902489086	23.340596454815337
88-89	23.083576096751	26.79023330521499	26.56172951159687	23.56446108643714
90-91	22.972537011855962	26.809464583277325	26.716996847937384	23.501001556929328
92-93	23.15072671592599	26.832571710942638	26.608642111993802	23.40805946113757
94-95	22.702641900797076	26.710286064234463	26.865510971152574	23.721561063815887
96-97	22.851246597557058	26.70646824359074	26.727950530843593	23.71433462800861
98-99	22.73438062884191	27.066859159425487	26.58126421895214	23.61749599278046
100	23.215757590071206	26.666934331565955	26.381753126365385	23.73555495199745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1790.0
1	1231.5
2	719.5
3	818.0
4	845.0
5	776.5
6	699.0
7	659.0
8	641.0
9	635.0
10	613.5
11	584.0
12	584.0
13	593.0
14	660.0
15	779.0
16	912.0
17	1143.0
18	1390.5
19	1775.0
20	2402.0
21	3255.5
22	4619.5
23	6539.0
24	8983.0
25	12119.5
26	16861.5
27	22311.0
28	27174.0
29	34909.5
30	44764.5
31	53550.5
32	64312.5
33	74481.0
34	82674.0
35	92506.5
36	105102.5
37	118824.5
38	124257.0
39	122648.5
40	121320.5
41	119339.5
42	118929.0
43	125303.5
44	134742.0
45	147863.0
46	159144.0
47	165322.0
48	170821.0
49	171152.0
50	166997.0
51	167384.0
52	168286.5
53	166713.0
54	163641.0
55	149662.0
56	133969.0
57	117599.5
58	102409.5
59	91618.5
60	77987.5
61	64311.0
62	51718.5
63	40080.5
64	29403.5
65	20422.0
66	14887.0
67	11265.0
68	8214.0
69	5930.0
70	4398.5
71	3365.5
72	2709.0
73	2178.5
74	1705.0
75	1282.5
76	959.0
77	699.5
78	455.5
79	307.0
80	209.5
81	138.0
82	93.5
83	58.5
84	48.0
85	40.5
86	27.0
87	25.5
88	22.0
89	12.5
90	14.0
91	14.0
92	10.0
93	9.0
94	10.0
95	8.5
96	8.0
97	7.5
98	7.5
99	6.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10001109663757746
2	0.06478928521868975
3	0.08177585781602623
4	0.08370775650254716
5	0.08467370584580762
6	0.09096415644704041
7	0.08653492531208999
8	0.08818410711765663
9	0.07246976048461443
10-11	0.08051541172177172
12-13	0.07042006309769588
14-15	0.07901936822672197
16-17	0.08043295263149339
18-19	0.08022091497077768
20-21	0.07223416308381919
22-23	0.07552074682491274
24-25	0.07846571433485318
26-27	0.07201034555306372
28-29	0.08017379549061864
30-31	0.08466192597576787
32-33	0.08612262986069832
34-35	0.08838436490833258
36-37	0.08871420126944592
38-39	0.08953879217222925
40-41	0.08578101362954524
42-43	0.09102305579723922
44-45	0.08778359153630474
46-47	0.08444988831505215
48-49	0.09368530642622538
50-51	0.08991574801350162
52-53	0.09581746290342226
54-55	0.08766579283590711
56-57	0.09383844473674229
58-59	0.09827945574173247
60-61	0.0963946765353706
62-63	0.09738418561871058
64-65	0.09932786417527129
66-67	0.10219037259493338
68-69	0.09895090833399889
70-71	0.10068254922984388
72-73	0.09796139925065891
74-75	0.10140112130226935
76-77	0.09877421028340247
78-79	0.10514711997491359
80-81	0.10078856806020173
82-83	0.10486440309395932
84-85	0.10140112130226935
86-87	0.09794961938061914
88-89	0.09701900964747796
90-91	0.09732528626851178
92-93	0.09213036358097683
94-95	0.09207146423077801
96-97	0.09013956554425709
98-99	0.10294428427747813
100	0.24452654228537488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4244529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.822764933678755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80309349414763	34.45940682429308
2	15.70976189914134	15.96827072328246
3	6.580373045557736	10.0329825741089
4	3.385843988592007	6.883118125372835
5	1.9452845610870917	4.9432369988621865
6	1.1809011680187762	3.6009997483274963
7	0.7979160556980484	2.8386610094885025
8	0.5601762751991247	2.2775765724695085
9	0.39427369293451653	1.8034271293989939
>10	1.5611918954849777	12.801894521537783
>50	0.05588001052905004	1.9416585394123105
>100	0.024546222924504004	2.097061421776277
>500	5.893149773469699E-4	0.18612168865751905
>1k	1.6837570781267505E-4	0.16558412301194875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2983636111332963E-4
2	0.0	0.0	0.0	0.0	3.2983636111332963E-4
3	0.0	0.0	0.0	0.0	3.2983636111332963E-4
4	0.0	0.0	0.0	0.0	3.533961011928532E-4
5	0.0	0.0	0.0	0.0	4.2407532143142384E-4
6	0.0	0.0	0.0	0.0	6.596727222266593E-4
7	0.0	0.0	0.0	0.0	7.067922023857064E-4
8	0.0	0.0	0.0	0.0	7.3035194246523E-4
9	0.0	0.0	0.0	0.0	7.539116825447535E-4
10-11	0.0	0.0	0.0	0.0	9.188298631014184E-4
12-13	0.0	0.0	0.0	0.0	0.0026740304990259226
14-15	0.0	0.0	0.0	2.3559740079523546E-5	0.0034161623115309143
16-17	0.0	0.0	0.0	2.3559740079523546E-5	0.0041347343839563825
18-19	0.0	0.0	0.0	2.3559740079523546E-5	0.004488130485149236
20-21	0.0	0.0	0.0	2.3559740079523546E-5	0.005077123987137324
22-23	0.0	0.0	0.0	1.295785704373795E-4	0.005430520088330178
24-25	0.0	0.0	0.0	4.4763506151094737E-4	0.005501199308568749
26-27	0.0	0.0	0.0	9.423896031809419E-4	0.005831035669682077
28-29	0.0	0.0	0.0	0.0033808227014116288	0.005960614240119458
30-31	0.0	0.0	0.0	0.011485373288767729	0.005984173980198982
32-33	0.0	0.0	0.0	0.032229724428788215	0.0062079915109544545
34-35	0.0	0.0	0.0	0.06556675664131403	0.006596727222266593
36-37	0.0	0.0	0.0	0.11830523480932748	0.006608507092306355
38-39	0.0	0.0	0.0	0.19964523743388252	0.006950123323459447
40-41	0.0	0.0	0.0	0.2896081049275432	0.006997242803618494
42-43	0.0	0.0	0.0	0.38663889444506094	0.007032582413737779
44-45	0.0	0.0	0.0	0.5076770591036132	0.0073152992946920615
46-47	0.0	0.0	0.0	0.6553377300520269	0.007692255135964438
48-49	0.0	0.0	0.0	0.817923496340819	0.008210569417713956
50-51	0.0	0.0	0.0	0.993313981362832	0.00857574538894657
52-53	0.0	0.0	0.0	1.1661246748461371	0.009223638241133468
54-55	0.0	0.0	0.0	1.3568761103999996	0.00950635512208775
56-57	0.0	0.0	0.0	1.588020720320205	0.009683053172684176
58-59	0.0	0.0	0.0	1.8488034832604514	0.009847971353240843
60-61	0.0	0.0	0.0	2.1248176181621092	0.010024669403837269
62-63	0.0	0.0	0.0	2.4044481731659744	0.010719681736183213
64-65	0.0	0.0	0.0	2.6990980624705356	0.010919939526859164
66-67	0.0	0.0	0.0	3.0365324397595117	0.01096705900701821
68-69	0.0	0.0	0.0	3.412463432338429	0.011049518097296544
70-71	0.0	0.0	0.0	3.8178558798867908	0.011285115498091778
72-73	0.0	0.0	0.0	4.23571142993722	0.011909448610199153
74-75	0.0	0.0	0.0	4.656924242948982	0.012451322632028194
76-77	0.0	0.0	0.0	5.123171499122753	0.012616240812584858
78-79	0.0	0.0	0.0	5.6340644627472205	0.012792938863181287
80-81	0.0	0.0	0.0	6.1903099260247725	0.013099215484215092
82-83	0.0	0.0	0.0	6.765933275517732	0.013829567426680322
84-85	1.1779870039761773E-5	0.0	0.0	7.337162733485859	0.014006265477276748
86-87	2.3559740079523546E-5	0.0	0.0	7.939644186669475	0.014559919369145552
88	2.3559740079523546E-5	0.0	0.0	8.411934516173643	0.015101793390974593
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4450	0.0	36.781605	1
GTATCAA	6730	0.0	35.712254	1
CAACGCA	7220	0.0	31.05828	5
ATCAACG	7435	0.0	30.28876	3
AACGCAG	8205	0.0	27.89318	6
TATCAAC	8610	0.0	27.411167	2
TCAACGC	8670	0.0	26.13695	4
GTACATG	15065	0.0	25.663395	1
ACGCAGA	8840	0.0	25.198446	7
TACATGG	15280	0.0	24.830027	2
GTACTAG	1705	0.0	23.99973	1
CGCAGAG	9295	0.0	23.964108	8
ACATGGG	15615	0.0	23.60484	3
CATGGGG	11680	0.0	21.89692	4
GTGATCG	1120	0.0	21.398556	8
TGATCGC	1150	0.0	20.838858	9
GCTATTG	2865	0.0	20.338991	8
GCAGAGT	10715	0.0	20.30441	9
GGCTATT	3265	0.0	19.14327	7
GGGCTAT	3325	0.0	19.080502	6
>>END_MODULE