FastQCFastQC Report
Mon 16 Jan 2017
ERR1146593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4244529
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147410.3472941285122566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134100.3159361144664108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73340.1727871337432257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82750.059.7280651
GTATCAA156800.044.9572071
ATCAACG195450.035.414413
CAACGCA196150.035.317245
TATCAAC204550.034.437412
TCAACGC208650.033.309184
AACGCAG210350.033.201216
ACGCAGA228550.030.2892767
CGCAGAG234150.029.4845838
GCAGAGT254350.026.9397249
GTACATG148300.024.8292941
TACATGG146750.024.5453242
GAGTACT172700.023.62080812-13
GTACTAG16650.023.4426961
ACATGGG149300.023.1806663
GTGGTAT26000.023.1515661
CAGAGTA249000.023.12086710-11
AGAGTAC239100.022.20107710-11
CATGGGG112350.021.8476814
GTACTTT191300.021.14004314-15
Produced by FastQC (version 0.11.5)