FastQCFastQC Report
Mon 16 Jan 2017
ERR1146592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286780
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT554541.6871832005792904No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC142600.4338592786861305No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT133090.4049251851356039No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA106150.3229604658662886No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88200.26834774460109895No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT41720.12693274268432933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11300.023.7220481
CATGGGG69750.020.6265754
CTAGTAC14700.019.830073
ATGGGGG47550.018.3904555
TGATCGC9250.018.2893839
GTGATCG9500.017.8083538
TGGGGGG42050.017.5470856
CTAGGCA19300.017.296214
CTATTGA19100.017.2227699
TATAGGT21750.017.0772424
GTACATG218150.016.9873921
TACATGG215600.016.966052
GTATAAG16700.016.896261
ACATGGG209550.016.760343
TTTGCGT6750.016.7169633
GCTATTG19400.016.7144578
GCATCGT14700.016.6238238
TACCTGG26250.016.2990382
TAGTACT18100.016.1050854
CGTTAAC3804.472895E-916.08851
Produced by FastQC (version 0.11.5)