##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146592_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3286780
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345679053663464	31.0	31.0	34.0	28.0	34.0
2	31.379313187983374	31.0	31.0	34.0	28.0	34.0
3	31.532485898052197	33.0	31.0	34.0	28.0	34.0
4	35.00599462087514	37.0	35.0	37.0	32.0	37.0
5	35.02596249216558	37.0	35.0	37.0	32.0	37.0
6	35.17801404414047	37.0	35.0	37.0	32.0	37.0
7	35.07883947206689	37.0	35.0	37.0	32.0	37.0
8	35.0792343874552	37.0	35.0	37.0	32.0	37.0
9	36.69281789471763	39.0	37.0	39.0	32.0	39.0
10-11	36.69462969836739	39.0	37.0	39.0	32.5	39.0
12-13	36.66864697363377	39.0	37.0	39.0	32.0	39.0
14-15	37.96452348499139	40.0	38.0	41.0	33.0	41.0
16-17	38.014056006182344	40.0	38.0	41.0	33.0	41.0
18-19	38.039057831677205	40.0	38.0	41.0	33.0	41.0
20-21	38.13066907429156	40.0	38.0	41.0	33.0	41.0
22-23	38.11944775737956	40.0	38.0	41.0	32.5	41.0
24-25	38.11691959912133	40.0	38.0	41.0	32.0	41.0
26-27	38.14584273970269	40.0	38.0	41.0	32.5	41.0
28-29	38.14363039205544	40.0	38.0	41.0	32.5	41.0
30-31	38.13054174602499	40.0	38.0	41.0	32.5	41.0
32-33	38.07259080315689	40.0	38.0	41.0	32.0	41.0
34-35	37.991050207193666	40.0	38.0	41.0	32.0	41.0
36-37	37.895674946300026	40.0	38.0	41.0	32.0	41.0
38-39	37.82181268597229	40.0	38.0	41.0	32.0	41.0
40-41	37.81374171681706	40.0	38.0	41.0	32.0	41.0
42-43	37.63147457389908	40.0	37.5	41.0	31.0	41.0
44-45	37.46653624520047	40.0	37.0	41.0	31.0	41.0
46-47	37.309861931738666	40.0	37.0	41.0	30.0	41.0
48-49	37.17266549632163	40.0	36.0	41.0	30.0	41.0
50-51	36.999477604220544	40.0	36.0	41.0	30.0	41.0
52-53	36.928526247573615	39.0	36.0	41.0	30.0	41.0
54-55	36.796241458205294	39.0	35.0	41.0	30.0	41.0
56-57	36.53124608279228	39.0	35.0	41.0	29.5	41.0
58-59	36.324650113484935	39.0	35.0	40.5	29.0	41.0
60-61	36.0109383652085	38.0	35.0	40.0	29.0	41.0
62-63	35.82097280621155	38.0	35.0	40.0	29.0	41.0
64-65	35.54211294945205	37.0	34.0	40.0	28.0	41.0
66-67	35.22230267921796	36.5	34.0	39.5	28.0	41.0
68-69	34.916071656758284	36.0	34.0	39.0	28.0	41.0
70-71	34.54973956273314	35.5	34.0	39.0	27.5	41.0
72-73	34.18137112919027	35.0	33.5	38.5	26.5	40.0
74-75	33.89391532137837	35.0	33.0	37.0	26.5	39.5
76-77	33.626849986917286	35.0	33.0	37.0	26.0	39.0
78-79	33.28554999117678	35.0	33.0	36.0	26.0	39.0
80-81	33.02172993020525	35.0	33.0	36.0	26.0	38.5
82-83	32.74770793907715	35.0	33.0	35.5	25.5	37.0
84-85	32.58085466626912	35.0	33.0	35.0	26.0	37.0
86-87	32.47017689045205	35.0	33.0	35.0	25.5	36.5
88-89	32.272132147573004	35.0	32.0	35.0	25.0	36.0
90-91	32.0893677094299	35.0	32.0	35.0	25.0	36.0
92-93	31.9323150621581	35.0	32.0	35.0	25.0	36.0
94-95	31.820016094779692	35.0	32.0	35.0	24.5	35.0
96-97	31.718149374159513	35.0	32.0	35.0	24.0	35.0
98-99	31.67048372571331	35.0	32.0	35.0	24.0	35.0
100	30.37741589032427	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23420556019552663
1101	2	0.24869707540441155
1101	3	0.19807581092573656
1101	4	0.331927488706107
1101	5	0.1917795597334475
1101	6	0.2318281728165843
1101	7	0.2862899556081757
1101	8	0.30401530826966905
1101	9	0.3837156615281714
1101	10-11	0.38387452455878446
1101	12-13	0.38825663473216565
1101	14-15	0.462914540973685
1101	16-17	0.46145873813963334
1101	18-19	0.427979792857748
1101	20-21	0.4603045701042703
1101	22-23	0.4260349898567739
1101	24-25	0.43695640775490574
1101	26-27	0.4070644986633951
1101	28-29	0.45180075858495883
1101	30-31	0.5173391936182838
1101	32-33	0.5008686237374818
1101	34-35	0.4513838533412411
1101	36-37	0.47670832833086507
1101	38-39	0.4919398720217103
1101	40-41	0.4597797506890302
1101	42-43	0.5008548600258749
1101	44-45	0.4662202724831488
1101	46-47	0.49213639888203886
1101	48-49	0.5070595346925089
1101	50-51	0.3906960313507284
1101	52-53	0.3808158856475643
1101	54-55	0.4009801252020111
1101	56-57	0.46482034346952616
1101	58-59	0.3762009908636088
1101	60-61	0.38214454760455396
1101	62-63	0.3621286775691175
1101	64-65	0.4261088775185513
1101	66-67	0.4207674890433992
1101	68-69	0.37018778924650064
1101	70-71	0.28200847426795406
1101	72-73	0.29520462529780644
1101	74-75	0.3921766568336551
1101	76-77	0.3314995268518217
1101	78-79	0.370221387717244
1101	80-81	0.27145569326314245
1101	82-83	0.30341152138103666
1101	84-85	0.24051501824985166
1101	86-87	0.32672081027914857
1101	88-89	0.2915063052946323
1101	90-91	0.2999274245502761
1101	92-93	0.333227149342715
1101	94-95	0.3015523484054796
1101	96-97	0.31138139220549377
1101	98-99	0.27053017430667836
1101	100	0.15726551020102164
1102	1	0.2985304581970567
1102	2	0.3389607633920946
1102	3	0.3362720805673973
1102	4	0.36860181450228424
1102	5	0.31744716122977223
1102	6	0.34135050937197775
1102	7	0.39906747579512114
1102	8	0.38188612600017535
1102	9	0.444663833036941
1102	10-11	0.5520296625528118
1102	12-13	0.5259314169035676
1102	14-15	0.6635267139949832
1102	16-17	0.5868054407589867
1102	18-19	0.5958427027996294
1102	20-21	0.5820355333732508
1102	22-23	0.5322911031733
1102	24-25	0.6566121713623971
1102	26-27	0.5425642102402648
1102	28-29	0.5209410154026628
1102	30-31	0.5331721341345173
1102	32-33	0.5191045545082105
1102	34-35	0.4453460455800311
1102	36-37	0.49389126276995654
1102	38-39	0.5345212987719918
1102	40-41	0.5809053576262713
1102	42-43	0.5286982502271229
1102	44-45	0.47123691656685907
1102	46-47	0.4796322751063897
1102	48-49	0.45111139426612823
1102	50-51	0.4798483631408743
1102	52-53	0.42710994305910077
1102	54-55	0.46063891344754637
1102	56-57	0.566682693070959
1102	58-59	0.5228753573884362
1102	60-61	0.5534189641613096
1102	62-63	0.5146878514720896
1102	64-65	0.5117741939233369
1102	66-67	0.5383608503594601
1102	68-69	0.5539469298805884
1102	70-71	0.4379573435227755
1102	72-73	0.440382321175278
1102	74-75	0.5130795959629708
1102	76-77	0.4364135240235285
1102	78-79	0.38324924975687935
1102	80-81	0.3913242319042354
1102	82-83	0.38819013223831433
1102	84-85	0.42009079079193157
1102	86-87	0.4035968307729476
1102	88-89	0.4129814283433859
1102	90-91	0.4474870496838008
1102	92-93	0.4942299079583812
1102	94-95	0.42540468476152427
1102	96-97	0.45288740043138986
1102	98-99	0.4277037968905866
1102	100	0.5349453748790154
1103	1	0.2823941964310315
1103	2	0.3044470923925431
1103	3	0.3333585888596424
1103	4	0.2915063416201704
1103	5	0.24147226208140893
1103	6	0.3207319526709469
1103	7	0.4082561804118825
1103	8	0.3133070134945726
1103	9	0.44242268556944
1103	10-11	0.4218189946908595
1103	12-13	0.4465947965539456
1103	14-15	0.5469870456848014
1103	16-17	0.5162092955326258
1103	18-19	0.4694419856324359
1103	20-21	0.43591271849203395
1103	22-23	0.4456707535543032
1103	24-25	0.4915516603807788
1103	26-27	0.4675978274522734
1103	28-29	0.48833231975049074
1103	30-31	0.49271942234608446
1103	32-33	0.48279796553465815
1103	34-35	0.45487092231692117
1103	36-37	0.48907279571482576
1103	38-39	0.44106544937709913
1103	40-41	0.38222763463210185
1103	42-43	0.41047772131631177
1103	44-45	0.3347510357958967
1103	46-47	0.2984355023587426
1103	48-49	0.27204371161261065
1103	50-51	0.3281226795908907
1103	52-53	0.28154741504474856
1103	54-55	0.23697239619071553
1103	56-57	0.3250869960740914
1103	58-59	0.37608019826696193
1103	60-61	0.36661932274490283
1103	62-63	0.4031907649637958
1103	64-65	0.3477221993020905
1103	66-67	0.25463158097353045
1103	68-69	0.28612980751393735
1103	70-71	0.31928858511847835
1103	72-73	0.3655279957606652
1103	74-75	0.33367574118933163
1103	76-77	0.32906256033012227
1103	78-79	0.3107481291831462
1103	80-81	0.2520369167989074
1103	82-83	0.33720402735260535
1103	84-85	0.3001894012805053
1103	86-87	0.30677926017680335
1103	88-89	0.3423852831170251
1103	90-91	0.36209933117191895
1103	92-93	0.34821915045053586
1103	94-95	0.48210571568935734
1103	96-97	0.3691805425201373
1103	98-99	0.30320805507077253
1103	100	0.4553846397826433
1104	1	0.2660937999049864
1104	2	0.22920780814374808
1104	3	0.19648256395224095
1104	4	0.2956495477025314
1104	5	0.19806918809462815
1104	6	0.28513784417148713
1104	7	0.259314555447844
1104	8	0.3143449215442047
1104	9	0.308690698885691
1104	10-11	0.3667149620713275
1104	12-13	0.4400228248078619
1104	14-15	0.5053872602765423
1104	16-17	0.43925943491255026
1104	18-19	0.4252262152336357
1104	20-21	0.43047395965395907
1104	22-23	0.4371075509520779
1104	24-25	0.44662592737099516
1104	26-27	0.4820015002417506
1104	28-29	0.42922583208976306
1104	30-31	0.516803979668353
1104	32-33	0.4835735421627092
1104	34-35	0.5042811689727742
1104	36-37	0.4623632539110716
1104	38-39	0.4170868417404492
1104	40-41	0.4293600378352238
1104	42-43	0.38535847182899374
1104	44-45	0.43068855090930924
1104	46-47	0.3646365305443524
1104	48-49	0.42546728250092514
1104	50-51	0.40159360126341426
1104	52-53	0.45133714605221087
1104	54-55	0.38825393865858615
1104	56-57	0.47324364604973823
1104	58-59	0.49365872194160687
1104	60-61	0.44761441714980776
1104	62-63	0.45054005340570313
1104	64-65	0.5285303735322415
1104	66-67	0.4189309992723693
1104	68-69	0.3556936039329912
1104	70-71	0.29885308454485227
1104	72-73	0.37011646474532967
1104	74-75	0.35412268432899907
1104	76-77	0.31455809822794834
1104	78-79	0.3654488549758881
1104	80-81	0.3457395167792541
1104	82-83	0.32142226076998526
1104	84-85	0.2815114439505493
1104	86-87	0.26901066525909556
1104	88-89	0.3355464178805363
1104	90-91	0.3394079249628206
1104	92-93	0.27384364282689333
1104	94-95	0.2444733845320286
1104	96-97	0.26979323085505413
1104	98-99	0.3054500252064223
1104	100	0.30255628176345084
1105	1	0.1196890661616159
1105	2	0.06108676374893918
1105	3	0.05587842192679204
1105	4	0.12080488422155611
1105	5	0.07556207334633314
1105	6	0.1288483878974347
1105	7	0.15415357502355675
1105	8	0.1572230596752675
1105	9	0.13704784821582194
1105	10-11	0.18231974971821074
1105	12-13	0.1589250241936
1105	14-15	0.17229547544550172
1105	16-17	0.21200834076028485
1105	18-19	0.18800390875455264
1105	20-21	0.19400695983097194
1105	22-23	0.19397792566137184
1105	24-25	0.2205207760631538
1105	26-27	0.2104696729828177
1105	28-29	0.15632808115314134
1105	30-31	0.2346306206854578
1105	32-33	0.21280932358298088
1105	34-35	0.1716312239797233
1105	36-37	0.12624298801833334
1105	38-39	0.17196238705579248
1105	40-41	0.17108434480169166
1105	42-43	0.18682534722310606
1105	44-45	0.18553195601540295
1105	46-47	0.18087099865323353
1105	48-49	0.1607152551322102
1105	50-51	0.1301999839474206
1105	52-53	0.08247694727593569
1105	54-55	0.10376446557490482
1105	56-57	0.13885960881686543
1105	58-59	0.12985859882746098
1105	60-61	0.15518011441392332
1105	62-63	0.17339155436991405
1105	64-65	0.2274933411558493
1105	66-67	0.18418339499745429
1105	68-69	0.15640327102239837
1105	70-71	0.14505133681239357
1105	72-73	0.20920034435265933
1105	74-75	0.1474853437446697
1105	76-77	0.1828429191748242
1105	78-79	0.1425804739456069
1105	80-81	0.11368305049314387
1105	82-83	0.1897234687629208
1105	84-85	0.14888692470375986
1105	86-87	0.09356454084244348
1105	88-89	0.10174588156867514
1105	90-91	0.08421445217211954
1105	92-93	0.1118085548275154
1105	94-95	0.15324669909616517
1105	96-97	0.10887452797661368
1105	98-99	0.08679806869172779
1105	100	0.2513161955741623
1106	1	0.05028831755714691
1106	2	0.1051335716707662
1106	3	0.10339965486468827
1106	4	0.16607750130704346
1106	5	0.09423436009301867
1106	6	0.1687868040577598
1106	7	0.18088693416278545
1106	8	0.19097086369292526
1106	9	0.2391858548681327
1106	10-11	0.2614646609068174
1106	12-13	0.302453072339695
1106	14-15	0.37660297252482167
1106	16-17	0.37730897358853355
1106	18-19	0.3423549751743664
1106	20-21	0.34598058527301134
1106	22-23	0.32389600654358475
1106	24-25	0.3518877674053371
1106	26-27	0.364052211266916
1106	28-29	0.35800885056580256
1106	30-31	0.3528137923232606
1106	32-33	0.40433427677331935
1106	34-35	0.42126073132651953
1106	36-37	0.3764124048810231
1106	38-39	0.34271190231951465
1106	40-41	0.3077710431477172
1106	42-43	0.3456113346225962
1106	44-45	0.34445246101690685
1106	46-47	0.39853519157389883
1106	48-49	0.3630926523217042
1106	50-51	0.3792740807265318
1106	52-53	0.3520732105591051
1106	54-55	0.3167270230908059
1106	56-57	0.38276436755219123
1106	58-59	0.3564337509041664
1106	60-61	0.355589622426308
1106	62-63	0.3001664248003877
1106	64-65	0.4430844654462618
1106	66-67	0.40697199918466964
1106	68-69	0.31448864591139625
1106	70-71	0.2957439360672893
1106	72-73	0.31099706513580117
1106	74-75	0.2382867393647956
1106	76-77	0.29207195641062356
1106	78-79	0.3769775706073588
1106	80-81	0.3027860738112764
1106	82-83	0.29191183193942294
1106	84-85	0.33347397493241715
1106	86-87	0.24381496602666175
1106	88-89	0.3221717138525477
1106	90-91	0.3797075432570054
1106	92-93	0.2568271560896669
1106	94-95	0.257496614584781
1106	96-97	0.23056268885125775
1106	98-99	0.2756857412394531
1106	100	0.5069694379212848
1107	1	0.11034275944235716
1107	2	0.10109017200151982
1107	3	0.1472755837740145
1107	4	0.10972010785118158
1107	5	0.1738440792942555
1107	6	0.17090678987039354
1107	7	0.10374291101857125
1107	8	0.13476880481535147
1107	9	0.2533315574205801
1107	10-11	0.23481901177066788
1107	12-13	0.23998731218312486
1107	14-15	0.2989330125656764
1107	16-17	0.19674406424666557
1107	18-19	0.26946566655212933
1107	20-21	0.2527821886164503
1107	22-23	0.2396713333444609
1107	24-25	0.32628074465854695
1107	26-27	0.30083499070889985
1107	28-29	0.2559559556164217
1107	30-31	0.2788362869923162
1107	32-33	0.21948356462730345
1107	34-35	0.2594299840539591
1107	36-37	0.2685919391764173
1107	38-39	0.2686717930938798
1107	40-41	0.17924439269111048
1107	42-43	0.2687242438448081
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2216	58-59	-0.6859269590989854
2216	60-61	-0.6808835311858985
2216	62-63	-0.6417512707969735
2216	64-65	-0.667982175039576
2216	66-67	-0.6840689281412367
2216	68-69	-0.6735179056339149
2216	70-71	-0.677223826107614
2216	72-73	-0.5046837337160852
2216	74-75	-0.5173676972698402
2216	76-77	-0.6616312598323262
2216	78-79	-0.5640848116690478
2216	80-81	-0.5702616895961796
2216	82-83	-0.6003953308343171
2216	84-85	-0.5212793537810256
2216	86-87	-0.6400110335052247
2216	88-89	-0.6700509075654253
2216	90-91	-0.5758535504111464
2216	92-93	-0.5236335139866206
2216	94-95	-0.6215356868348181
2216	96-97	-0.5741645136657709
2216	98-99	-0.6195453920080816
2216	100	-0.6092471282507681
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1000.0
1	271.0
2	191.0
3	175.0
4	137.0
5	171.0
6	277.0
7	705.0
8	2025.0
9	2899.0
10	3224.0
11	3885.0
12	4495.0
13	5391.0
14	7035.0
15	8261.0
16	8933.0
17	9741.0
18	10655.0
19	11883.0
20	13191.0
21	15101.0
22	17292.0
23	20061.0
24	24706.0
25	29350.0
26	31836.0
27	30788.0
28	34227.0
29	42416.0
30	50453.0
31	59461.0
32	77134.0
33	104846.0
34	156655.0
35	245231.0
36	433926.0
37	785914.0
38	806320.0
39	226205.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.087957550084624	19.556916978352017	13.249061289303798	23.106064182259566
2	14.5849304183424	22.528899754310228	37.81013386591488	25.076035961432492
3	20.901060172888233	25.588116270975767	30.202884393744746	23.307939162391254
4	12.120722142847573	17.27053316622026	40.138867065935365	30.469877624996805
5	12.613612002981744	39.49346281833285	34.73945061851702	13.15347456016838
6	29.092915705094825	37.8820325694966	18.002726204637106	15.022325520771474
7	25.77412608196765	33.060400166634594	23.18931053720166	17.9761632141961
8	25.167740782212732	32.40712628437837	20.810089083122627	21.61504385028627
9	24.820958204348035	17.66047384989567	22.337711449875453	35.18085649588084
10-11	24.986971092191684	25.574874388844123	27.996995167694948	21.441159351269253
12-13	27.00125198133963	23.95626103323779	26.976209309845284	22.06627767557729
14-15	23.24538345746983	25.58591255132896	26.85702706064533	24.31167693055588
16-17	21.767115574988257	28.283218753968054	27.36270649483365	22.58695917621004
18-19	21.54326492164357	29.98087582654842	26.89919059116853	21.576668660639477
20-21	23.62198191390035	27.145621555344896	25.570269632107827	23.662126898646928
22-23	22.82182793428363	27.226136496676745	25.95377358504929	23.99826198399034
24-25	24.310696048548923	27.34300921710905	25.488153563674786	22.858141170667242
26-27	23.54578274331393	26.0048710131482	27.811801321853764	22.637544921684107
28-29	22.060094161760727	29.31598208846784	26.21444173994174	22.40948200982969
30-31	23.141547458378607	26.86263965153501	25.984975736078695	24.01083715400769
32-33	23.159664638361342	27.61917537238212	26.267396458677567	22.95376353057897
34-35	24.29066089567247	26.927135919794832	26.345062555224114	22.437140629308587
36-37	23.563276933451917	26.159611314205094	27.929657969229698	22.347453783113288
38-39	23.158601728942692	27.53202825679614	27.0087683529243	22.30060166133687
40-41	22.743027429847974	27.938348922672894	26.58774652330727	22.73087712417186
42-43	22.536252263649008	27.73165505955721	25.178065600864148	24.554027075929636
44-45	23.355879768519273	26.533935904742517	25.917960372621422	24.19222395411679
46-47	24.30079360958757	27.50282094308341	26.470852893927514	21.725532553401507
48-49	23.752884956382854	26.831674167768977	26.83062350080102	22.584817375047148
50-51	21.356891881932977	27.53399911443034	28.314521576167824	22.79458742746886
52-53	22.55962947634114	28.358350319423774	25.48387136070538	23.59814884352971
54-55	21.99114722882317	26.79609985959994	26.41607110963858	24.796681801938316
56-57	23.95286044758029	25.861459880156364	26.208349220309827	23.97733045195352
58-59	24.947300344712012	26.10120313431038	26.15178993048655	22.799706590491052
60-61	23.248879020048964	26.99194551198397	27.798962756634587	21.96021271133247
62-63	22.111454107007003	27.186441483228496	27.249484237179633	23.45262017258487
64-65	21.464108366788192	29.000965309026043	26.211954695195182	23.32297162899058
66-67	23.159830190768126	26.67586261088775	25.426795923591634	24.73751127475249
68-69	22.16170960297908	26.88538415175111	26.367069304681173	24.585836940588635
70-71	23.83318907203948	26.796875428291578	25.72374804851322	23.646187451155726
72-73	23.33402974956121	25.64173587937421	28.045437808761868	22.978796562302705
74-75	22.26717947374737	28.839992251853737	26.129724800707564	22.76310347369133
76-77	22.620468337350175	26.70386617314354	26.22690074651287	24.44876474299342
78-79	23.011482006710477	26.928988092192306	26.731499282412653	23.32803061868456
80-81	23.69043322536582	26.530481407201993	26.537851827148423	23.241233540283762
82-83	23.044507864182943	25.799713088290833	28.172110476236888	22.983668571289336
84-85	22.810386461107573	27.31220859784176	26.746387591653146	23.131017349397517
86-87	22.41599009831636	27.63587557355271	26.526047653070734	23.422086675060193
88-89	22.45928924054295	27.11079234847862	25.550642144277653	24.879276266700774
90-91	23.144080000572558	25.930459218160717	26.184119421603576	24.741341359663156
92-93	24.13855480575705	26.96661720006444	26.44172102359018	22.453106970588337
94-95	22.86334010157695	26.62640741781228	26.878636788944238	23.631615691666532
96-97	21.576380677203545	26.9292693852704	28.113929451188557	23.380420486337496
98-99	22.8345702460536	27.753159998038562	25.696225972030046	23.716043783877787
100	22.886045278833947	25.318899609530597	25.630067302747683	26.16498780888777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2181.0
1	1410.0
2	660.0
3	695.5
4	664.0
5	584.0
6	509.0
7	501.0
8	551.5
9	550.0
10	488.5
11	438.5
12	440.5
13	466.0
14	513.0
15	581.0
16	691.0
17	835.5
18	1007.0
19	1256.0
20	1650.0
21	2293.0
22	3264.5
23	4529.0
24	6143.5
25	8277.5
26	11229.5
27	15088.0
28	19237.0
29	24618.0
30	30948.5
31	37073.0
32	44922.0
33	51995.5
34	56989.5
35	62404.5
36	69542.5
37	78525.5
38	82877.5
39	82750.0
40	82542.5
41	82082.0
42	82721.0
43	86521.5
44	93195.5
45	103640.0
46	118851.5
47	164876.5
48	247785.5
49	247782.0
50	182252.5
51	145631.0
52	120574.5
53	110174.0
54	105022.0
55	95866.0
56	86372.5
57	75303.0
58	64427.5
59	57950.0
60	49716.5
61	39898.0
62	31497.0
63	24683.0
64	18823.0
65	13533.0
66	10003.5
67	7888.5
68	6126.5
69	4659.0
70	3778.0
71	3086.5
72	2454.5
73	2086.0
74	1688.5
75	1311.0
76	1009.5
77	756.0
78	553.5
79	363.5
80	265.0
81	195.0
82	130.5
83	89.5
84	65.5
85	51.0
86	35.0
87	23.0
88	21.0
89	21.0
90	17.0
91	12.0
92	8.0
93	6.5
94	7.5
95	7.0
96	6.0
97	7.5
98	7.0
99	10.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10000669348115786
2	0.0654135658608121
3	0.08193429435496138
4	0.08445956224633228
5	0.08506806053340961
6	0.0941346850108617
7	0.08969264751519725
8	0.09106176866112121
9	0.07578846165548044
10-11	0.08325777812935456
12-13	0.0725938456483245
14-15	0.08194950681213832
16-17	0.08333384041523922
18-19	0.08264927984227724
20-21	0.074069453994487
22-23	0.07835936691838213
24-25	0.08123452132482248
26-27	0.07463231491003353
28-29	0.08368372693030869
30-31	0.08798885231138075
32-33	0.08890159974199673
34-35	0.09132038043312908
36-37	0.09116825586135975
38-39	0.09246131472139905
40-41	0.08859735059845807
42-43	0.09396734798191543
44-45	0.09019465860203603
46-47	0.08703046750923396
48-49	0.095884117586209
50-51	0.09182239151996788
52-53	0.09769439999026404
54-55	0.08973828488672804
56-57	0.09638612867304779
58-59	0.10084337862588917
60-61	0.09945904502278825
62-63	0.10021966788163492
64-65	0.10269929840147499
66-67	0.10492031714930722
68-69	0.10195388799980529
70-71	0.1030187600021906
72-73	0.10049349211081972
74-75	0.10387065760409885
76-77	0.1008129537115353
78-79	0.10819099544234782
80-81	0.10298833508783672
82-83	0.10770419681268596
84-85	0.10373374548950645
86-87	0.10034136753905037
88-89	0.0988809716500648
90-91	0.09898745885030334
92-93	0.09380001095296917
94-95	0.09317630020871491
96-97	0.0916094171194908
98-99	0.10560487772226922
100	0.24832815095625504
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3286780.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.243856567662235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83914614070417	41.12349777621795
2	15.130501444475511	17.32256508968725
3	5.829945133112263	10.011856289916569
4	2.801978203330782	6.4158415350873295
5	1.5298635122173754	4.378764373073571
6	0.8710953615204076	2.991891475897805
7	0.5519923471036086	2.211871952083234
8	0.3570371037870984	1.6350544606817756
9	0.25263682749272315	1.3015715685032405
>10	0.8237452400728417	6.9681743680321135
>50	0.00838205347769914	0.323269012116077
>100	0.0028353056844399377	0.23761077866537136
>500	0.0	0.0
>1k	4.5890564789784574E-4	0.5921589681549052
>5k	7.648427464964096E-5	0.38616207756940957
>10k+	3.059370985985638E-4	4.099710274313421
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	55454	1.6871832005792904	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	14260	0.4338592786861305	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	13309	0.4049251851356039	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10615	0.3229604658662886	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8820	0.26834774460109895	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	4172	0.12693274268432933	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.127474306159828E-5
2	0.0	0.0	0.0	0.0	1.2169965741546438E-4
3	0.0	0.0	0.0	3.0424914353866095E-5	1.2169965741546438E-4
4	0.0	0.0	0.0	3.0424914353866095E-5	1.2169965741546438E-4
5	0.0	0.0	0.0	3.0424914353866095E-5	1.2169965741546438E-4
6	0.0	0.0	0.0	1.5212457176933046E-4	1.8254948612319655E-4
7	0.0	0.0	0.0	1.5212457176933046E-4	1.8254948612319655E-4
8	0.0	0.0	0.0	1.5212457176933046E-4	1.8254948612319655E-4
9	0.0	0.0	0.0	2.4339931483092876E-4	1.8254948612319655E-4
10-11	0.0	0.0	0.0	3.4988651506946006E-4	2.1297440047706264E-4
12-13	0.0	0.0	0.0	4.411612581310584E-4	5.324360011926566E-4
14-15	0.0	0.0	0.0	4.5637371530799143E-4	6.389232014311879E-4
16-17	0.0	0.0	0.0	5.324360011926566E-4	6.997730301389201E-4
18-19	0.0	0.0	0.0	8.062602303774515E-4	6.997730301389201E-4
20-21	0.0	0.0	0.0	0.001004022173677581	9.127474306159829E-4
22-23	0.0	0.0	0.0	0.0011865716598007776	9.73597259323715E-4
24-25	0.0	0.0	0.0	0.0017494325753473006	9.73597259323715E-4
26-27	0.0	0.0	0.0	0.0031489786356251404	0.0011257218310930454
28-29	0.0	0.0	0.0	0.007347616816458662	0.0011865716598007776
30-31	0.0	0.0	0.0	0.01842228564126592	0.0012017841169777105
32-33	0.0	0.0	0.0	0.047736690621215896	0.0012778464028623759
34-35	0.0	0.0	0.0	0.09051412020275162	0.0013995460602778402
36-37	0.0	0.0	0.0	0.15188117245449953	0.0013995460602778402
38-39	3.0424914353866095E-5	0.0	0.0	0.24460109894790644	0.0014299709746317063
40-41	3.0424914353866095E-5	0.0	0.0	0.3531571933625007	0.0014603958889855724
42-43	3.0424914353866095E-5	0.0	0.0	0.4649839660701355	0.0014756083461625056
44-45	3.0424914353866095E-5	0.0	0.0	0.6061251437577203	0.001612520460754903
46-47	3.0424914353866095E-5	0.0	0.0	0.7784670711151949	0.0017494325753473006
48-49	3.0424914353866095E-5	0.0	0.0	0.9484054302387139	0.0017950699468780996
50-51	3.0424914353866095E-5	0.0	0.0	1.1338300707683509	0.0018254948612319657
52-53	3.0424914353866095E-5	0.0	0.0	1.3228904885632748	0.001977619433001296
54-55	3.0424914353866095E-5	0.0	0.0	1.5426648573984263	0.002008044347355162
56-57	3.0424914353866095E-5	0.0	0.0	1.8219047213382096	0.0020688941760628942
58-59	3.0424914353866095E-5	0.0	0.0	2.1002166253901997	0.0021297440047706264
60-61	3.0424914353866095E-5	0.0	0.0	2.3937409866191226	0.0021601689191244927
62-63	3.0424914353866095E-5	0.0	0.0	2.692011634487249	0.002221018747832225
64-65	3.0424914353866095E-5	0.0	0.0	3.011853546632266	0.002221018747832225
66-67	3.0424914353866095E-5	0.0	0.0	3.383433025636033	0.002221018747832225
68-69	3.0424914353866095E-5	0.0	0.0	3.807662818929165	0.002221018747832225
70-71	3.0424914353866095E-5	0.0	0.0	4.264371208295049	0.00229708103371689
72-73	3.0424914353866095E-5	0.0	0.0	4.70040586835748	0.002479630519840087
74-75	3.0424914353866095E-5	0.0	0.0	5.162423405278114	0.002662180005963283
76-77	3.0424914353866095E-5	0.0	0.0	5.6433043890981445	0.0027838796633787473
78-79	3.0424914353866095E-5	0.0	0.0	6.2399369595774585	0.0028599419492634126
80-81	3.0424914353866095E-5	0.0	0.0	6.865594898350361	0.0028903668636172785
82-83	3.0424914353866095E-5	0.0	0.0	7.471233243478419	0.0029664291495019443
84-85	3.0424914353866095E-5	0.0	0.0	8.079107819811487	0.0030120665210327433
86-87	3.0424914353866095E-5	0.0	0.0	8.698939387485623	0.003148978635625141
88	3.0424914353866095E-5	0.0	0.0	9.212664066350653	0.0032858907502175383
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1130	0.0	23.722048	1
CATGGGG	6975	0.0	20.626575	4
CTAGTAC	1470	0.0	19.83007	3
ATGGGGG	4755	0.0	18.390455	5
TGATCGC	925	0.0	18.289383	9
GTGATCG	950	0.0	17.808353	8
TGGGGGG	4205	0.0	17.547085	6
CTAGGCA	1930	0.0	17.29621	4
CTATTGA	1910	0.0	17.222769	9
TATAGGT	2175	0.0	17.077242	4
GTACATG	21815	0.0	16.987392	1
TACATGG	21560	0.0	16.96605	2
GTATAAG	1670	0.0	16.89626	1
ACATGGG	20955	0.0	16.76034	3
TTTGCGT	675	0.0	16.716963	3
GCTATTG	1940	0.0	16.714457	8
GCATCGT	1470	0.0	16.623823	8
TACCTGG	2625	0.0	16.299038	2
TAGTACT	1810	0.0	16.105085	4
CGTTAAC	380	4.472895E-9	16.0885	1
>>END_MODULE