FastQCFastQC Report
Mon 16 Jan 2017
ERR1146592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286780
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT573791.7457511607104828No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC156030.47471993866337264No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA116100.3532332556483853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100850.30683526125873956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96330.2930831999707921No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86390.26284083510304923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50540.15376751714443923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10500.023.7351341
GAGTACT115050.022.28376412-13
GTACTTT126050.020.3206414-15
AGTACTT123450.019.96801212-13
CATGGGG68550.019.0004634
GGGCTAT18900.018.8995746
GTATAAG14500.018.8089751
TAGGCAT16700.018.57585
ACTTTTT142050.018.09810416-17
GTGATCG8200.017.767588
TATCACG1600.00127545417.6328772
GAGTACG8300.017.5625951
TACGCAG7500.017.5545064
TACTTTT141650.017.4191314-15
CTATTGA21100.017.373719
AGGGCTA22550.017.300225
TGATCGC8200.017.1944319
ACGTGCG8850.016.993679
CTAGTAC13750.016.7565773
TAGTACT17150.016.7246724
Produced by FastQC (version 0.11.5)