##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146592_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286780 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3569314039881 33.0 31.0 34.0 30.0 34.0 2 32.419058470600405 34.0 31.0 34.0 30.0 34.0 3 32.512482429611964 34.0 31.0 34.0 30.0 34.0 4 36.001246204491935 37.0 35.0 37.0 35.0 37.0 5 36.05357918692459 37.0 35.0 37.0 35.0 37.0 6 36.13678858944012 37.0 36.0 37.0 35.0 37.0 7 36.115714772512916 37.0 36.0 37.0 35.0 37.0 8 36.125423362683236 37.0 36.0 37.0 35.0 37.0 9 37.911496358137754 39.0 38.0 39.0 35.0 39.0 10-11 37.85540818065097 39.0 38.0 39.0 35.0 39.0 12-13 37.83702940263723 39.0 38.0 39.0 35.0 39.0 14-15 39.146062711833466 40.0 39.0 41.0 36.0 41.0 16-17 39.12063858852737 40.0 39.0 41.0 36.0 41.0 18-19 39.234815837993416 40.0 39.0 41.0 36.0 41.0 20-21 39.28601321049781 41.0 39.0 41.0 36.0 41.0 22-23 39.24417834476296 40.5 39.0 41.0 36.0 41.0 24-25 39.214825148017205 41.0 39.0 41.0 36.0 41.0 26-27 39.11153225953669 40.0 39.0 41.0 35.5 41.0 28-29 39.072113284126104 40.0 39.0 41.0 35.0 41.0 30-31 38.96759381522341 40.0 38.5 41.0 35.0 41.0 32-33 38.842224304638584 40.0 38.0 41.0 35.0 41.0 34-35 38.748769920712675 40.0 38.0 41.0 35.0 41.0 36-37 38.66506656971261 40.0 38.0 41.0 35.0 41.0 38-39 38.57952175077128 40.0 38.0 41.0 35.0 41.0 40-41 38.53610402886716 40.0 38.0 41.0 35.0 41.0 42-43 38.437075344257906 40.0 38.0 41.0 34.0 41.0 44-45 38.28640067178211 40.0 38.0 41.0 34.0 41.0 46-47 38.03667753850273 40.0 38.0 41.0 33.0 41.0 48-49 37.72070750095838 40.0 37.0 41.0 33.0 41.0 50-51 37.57354386359903 40.0 37.0 41.0 33.0 41.0 52-53 37.534317325771724 40.0 37.0 41.0 33.0 41.0 54-55 37.38053946415641 40.0 36.5 41.0 32.5 41.0 56-57 37.187242985535995 39.5 36.0 41.0 32.0 41.0 58-59 36.99374387698599 39.0 35.0 41.0 32.0 41.0 60-61 36.70194399990264 39.0 35.0 41.0 31.0 41.0 62-63 36.496354486762115 38.5 35.0 41.0 31.0 41.0 64-65 36.22662971053737 37.5 35.0 40.0 31.0 41.0 66-67 35.91111011385003 37.0 35.0 40.0 31.0 41.0 68-69 35.559523454566474 36.5 35.0 39.0 30.5 41.0 70-71 35.180908366242946 36.0 35.0 39.0 30.0 41.0 72-73 34.83408290180663 35.5 34.5 38.5 29.5 40.5 74-75 34.54887945040434 35.0 34.0 37.0 30.0 40.0 76-77 34.26609523606692 35.0 34.0 37.0 29.5 39.0 78-79 33.98169378540699 35.0 34.0 36.5 29.0 39.0 80-81 33.72727623996738 35.0 34.0 36.0 29.0 38.5 82-83 33.46925319005227 35.0 34.0 36.0 29.0 37.0 84-85 33.28863522961683 35.0 34.0 35.0 29.0 37.0 86-87 33.18291777971145 35.0 34.0 35.0 29.0 36.5 88-89 33.00045698221359 35.0 34.0 35.0 29.0 36.0 90-91 32.90018559197756 35.0 34.0 35.0 29.0 36.0 92-93 32.788605413200756 35.0 34.0 35.0 29.0 36.0 94-95 32.70391796834592 35.0 34.0 35.0 28.0 35.5 96-97 32.6507054016393 35.0 34.0 35.0 28.0 35.0 98-99 32.63117169387668 35.0 34.0 35.0 27.0 35.0 100 31.838698361314112 35.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3557068068826936 1101 2 0.3249680675966644 1101 3 0.35203861747376664 1101 4 0.23540539455234466 1101 5 0.24458815569570191 1101 6 0.22231179371782872 1101 7 0.22039976645448434 1101 8 0.24243320217843944 1101 9 0.2924090232448151 1101 10-11 0.3023379118025957 1101 12-13 0.3221739201124052 1101 14-15 0.4445344006238017 1101 16-17 0.5047613100768729 1101 18-19 0.47470219249795065 1101 20-21 0.4156711683558072 1101 22-23 0.3973220744242454 1101 24-25 0.44526694685980317 1101 26-27 0.38360069512551576 1101 28-29 0.44724424079171854 1101 30-31 0.4979189296744764 1101 32-33 0.4283038269666051 1101 34-35 0.41089376867245875 1101 36-37 0.4719604542405591 1101 38-39 0.4639292309632168 1101 40-41 0.48234173560077664 1101 42-43 0.5134832943092675 1101 44-45 0.5604541374139131 1101 46-47 0.5192296676273784 1101 48-49 0.4782162120841775 1101 50-51 0.45846655072933373 1101 52-53 0.4249293117447408 1101 54-55 0.45954562144251554 1101 56-57 0.4459972678409514 1101 58-59 0.43499389652244247 1101 60-61 0.42951087713585423 1101 62-63 0.4554173061644704 1101 64-65 0.3811129098748225 1101 66-67 0.3601852969688153 1101 68-69 0.2588820436809698 1101 70-71 0.31353067628133147 1101 72-73 0.2959571162092445 1101 74-75 0.3446712708394841 1101 76-77 0.33565985713789104 1101 78-79 0.28332941122504707 1101 80-81 0.26307025508754833 1101 82-83 0.33524101502594306 1101 84-85 0.3788753214208356 1101 86-87 0.3029706883010448 1101 88-89 0.4311547864099552 1101 90-91 0.3253284651784014 1101 92-93 0.3017412747981041 1101 94-95 0.385121304097197 1101 96-97 0.3852368009576139 1101 98-99 0.37913610677868803 1101 100 0.46329551777834155 1102 1 0.2739443667993555 1102 2 0.2426286740548136 1102 3 0.26527223421418 1102 4 0.20294337562467035 1102 5 0.18130194540854205 1102 6 0.1833176498543807 1102 7 0.15245655676689296 1102 8 0.17102955790760177 1102 9 0.24100904026510506 1102 10-11 0.2403490697373627 1102 12-13 0.30422314347067925 1102 14-15 0.4496037239987629 1102 16-17 0.4799411109823808 1102 18-19 0.3844299300579692 1102 20-21 0.33256500441785875 1102 22-23 0.40103272224340714 1102 24-25 0.387667956415811 1102 26-27 0.3848556198085973 1102 28-29 0.4419630748548471 1102 30-31 0.3900001764684333 1102 32-33 0.4688691320316849 1102 34-35 0.47109708822878815 1102 36-37 0.4978129638005413 1102 38-39 0.48040246870426984 1102 40-41 0.4960343320542506 1102 42-43 0.5452359380808645 1102 44-45 0.601909202675138 1102 46-47 0.6200927719949689 1102 48-49 0.631980686027596 1102 50-51 0.6740044872145106 1102 52-53 0.6348081633083211 1102 54-55 0.5946380465355645 1102 56-57 0.66394853751369 1102 58-59 0.6801255368730423 1102 60-61 0.6401733264581679 1102 62-63 0.5331894641033088 1102 64-65 0.5574840880538687 1102 66-67 0.5278016378170136 1102 68-69 0.5632699017420606 1102 70-71 0.5673554179062847 1102 72-73 0.6077759161674834 1102 74-75 0.5964629026456407 1102 76-77 0.5236653880883395 1102 78-79 0.4998141280141013 1102 80-81 0.5614451712990771 1102 82-83 0.5847330859083613 1102 84-85 0.4562628615841007 1102 86-87 0.5424109287629832 1102 88-89 0.5823857240627532 1102 90-91 0.5108889374678043 1102 92-93 0.5102486963081034 1102 94-95 0.5005305916438161 1102 96-97 0.5401797797780787 1102 98-99 0.5147219380107444 1102 100 0.5317960668648283 1103 1 0.20738228701451078 1103 2 0.18973759382173938 1103 3 0.2072265148058463 1103 4 0.14467354146958655 1103 5 0.16471745414902017 1103 6 0.15664799499108994 1103 7 0.18741845725994466 1103 8 0.19276868834238314 1103 9 0.20694359875905377 1103 10-11 0.24953777435412405 1103 12-13 0.23015321966968827 1103 14-15 0.32465975268545577 1103 16-17 0.4220074489474186 1103 18-19 0.3147302438186159 1103 20-21 0.2783292357042697 1103 22-23 0.3398493963805649 1103 24-25 0.3027284673974364 1103 26-27 0.332973055935895 1103 28-29 0.33438799641468364 1103 30-31 0.3651234395791434 1103 32-33 0.3690260123544107 1103 34-35 0.37181425541839275 1103 36-37 0.38362650033124623 1103 38-39 0.37103447229010555 1103 40-41 0.40930192506187524 1103 42-43 0.42880832714406836 1103 44-45 0.3861987589279323 1103 46-47 0.4554804905068508 1103 48-49 0.5349390006846946 1103 50-51 0.4625522236555639 1103 52-53 0.4282864241778839 1103 54-55 0.473279911170259 1103 56-57 0.4427472824711032 1103 58-59 0.42373826659066083 1103 60-61 0.36955476975713 1103 62-63 0.423933525062516 1103 64-65 0.4510295833474558 1103 66-67 0.38481642939030536 1103 68-69 0.3958561859195697 1103 70-71 0.37685788316849766 1103 72-73 0.392289587167042 1103 74-75 0.3557636646357807 1103 76-77 0.36275099992160165 1103 78-79 0.42159808139822985 1103 80-81 0.4269763232488728 1103 82-83 0.3759701993104301 1103 84-85 0.4122243138476662 1103 86-87 0.4346117355760697 1103 88-89 0.49520508757687054 1103 90-91 0.4307679155045605 1103 92-93 0.4090608881503286 1103 94-95 0.47610208424802636 1103 96-97 0.4227436524809036 1103 98-99 0.39426026163244643 1103 100 0.37379517040584176 1104 1 0.2497407220304595 1104 2 0.1943740019229736 1104 3 0.22567881177010207 1104 4 0.16434179181398179 1104 5 0.15228170328484225 1104 6 0.18547049760742595 1104 7 0.19170844728322578 1104 8 0.18825732579951193 1104 9 0.2519088447692326 1104 10-11 0.20081360800364223 1104 12-13 0.2030608878841349 1104 14-15 0.3835927570844788 1104 16-17 0.3589933623758057 1104 18-19 0.3038673098050779 1104 20-21 0.2662396037660031 1104 22-23 0.29660764013863883 1104 24-25 0.3053097756791061 1104 26-27 0.3994334738812242 1104 28-29 0.3929972933624839 1104 30-31 0.3668761216019547 1104 32-33 0.3816931074732963 1104 34-35 0.32558613901433375 1104 36-37 0.3587241360827065 1104 38-39 0.3527728847599576 1104 40-41 0.3884959246699822 1104 42-43 0.44008065074282143 1104 44-45 0.44339064851689614 1104 46-47 0.4608036520055805 1104 48-49 0.4572336814121769 1104 50-51 0.4590923057927512 1104 52-53 0.4465823892648473 1104 54-55 0.4634125302614436 1104 56-57 0.4347534659440555 1104 58-59 0.39427946755652243 1104 60-61 0.43556835425188467 1104 62-63 0.4246168631407343 1104 64-65 0.34071846022595054 1104 66-67 0.36250651683743484 1104 68-69 0.3814828770635188 1104 70-71 0.35315935664193887 1104 72-73 0.32196260400082366 1104 74-75 0.31309984523188206 1104 76-77 0.25194076652815767 1104 78-79 0.3223643344539511 1104 80-81 0.3181002075986825 1104 82-83 0.327619549228082 1104 84-85 0.22747924873472414 1104 86-87 0.2938265412094978 1104 88-89 0.3288006110655388 1104 90-91 0.29803930973760373 1104 92-93 0.35580429396653557 1104 94-95 0.3204945787224389 1104 96-97 0.3672650814903733 1104 98-99 0.3590308194862004 1104 100 0.4152812780337669 1105 1 0.18228361124980097 1105 2 0.15892943169320972 1105 3 0.1444221648038848 1105 4 0.12256233703777042 1105 5 0.1419099708923497 1105 6 0.13420388224614044 1105 7 0.1628028249299831 1105 8 0.12980332386217697 1105 9 0.1246673762457462 1105 10-11 0.1759280473389424 1105 12-13 0.16072878115328137 1105 14-15 0.23732245346398173 1105 16-17 0.27652325230086916 1105 18-19 0.2297450615023635 1105 20-21 0.16141096578308378 1105 22-23 0.1887558034512793 1105 24-25 0.21041821425656337 1105 26-27 0.21038664120611372 1105 28-29 0.19040297511290305 1105 30-31 0.26732237514288926 1105 32-33 0.2559144293209812 1105 34-35 0.2882792913420289 1105 36-37 0.23840558411534118 1105 38-39 0.29209059671513415 1105 40-41 0.2742216571685887 1105 42-43 0.261122627405598 1105 44-45 0.29535939480457785 1105 46-47 0.287483641168528 1105 48-49 0.4361294198880685 1105 50-51 0.33524851988739357 1105 52-53 0.30545196011675557 1105 54-55 0.26696814362294674 1105 56-57 0.2654714298559284 1105 58-59 0.2965194218042697 1105 60-61 0.28632551560230723 1105 62-63 0.25258365800825544 1105 64-65 0.2918319576582533 1105 66-67 0.2572973143606916 1105 68-69 0.23751476559779405 1105 70-71 0.20404256677504407 1105 72-73 0.24822188275480528 1105 74-75 0.1451512874093197 1105 76-77 0.16834668821497445 1105 78-79 0.21084318459980977 1105 80-81 0.276948907270139 1105 82-83 0.2361062783032608 1105 84-85 0.2029305146457716 1105 86-87 0.28098325255004397 1105 88-89 0.28898253075399083 1105 90-91 0.24521613629457306 1105 92-93 0.204904321153073 1105 94-95 0.30004145576935315 1105 96-97 0.21078985058093025 1105 98-99 0.23363852815926833 1105 100 0.2483714194647746 1106 1 0.13484750273192958 1106 2 0.12323410004952251 1106 3 0.0736518938945423 1106 4 0.08789839458608384 1106 5 0.11478199175658688 1106 6 0.12609008876219008 1106 7 0.08653511038053807 1106 8 0.13833809788837215 1106 9 0.12915234723163138 1106 10-11 0.13626343930536677 1106 12-13 0.14362140002533863 1106 14-15 0.264588584629152 1106 16-17 0.35481017589382446 1106 18-19 0.27796900878826847 1106 20-21 0.20368912121046634 1106 22-23 0.2234589364152768 1106 24-25 0.24067871950327913 1106 26-27 0.26191980032047013 1106 28-29 0.2573426471211562 1106 30-31 0.25609812484206884 1106 32-33 0.2663384487699858 1106 34-35 0.24475058637170122 1106 36-37 0.27701809109957765 1106 38-39 0.2824438192699503 1106 40-41 0.24626769967125028 1106 42-43 0.26000747123031687 1106 44-45 0.23586718999452216 1106 46-47 0.3228801177882161 1106 48-49 0.39837621414795166 1106 50-51 0.3684101739634684 1106 52-53 0.37135853519001216 1106 54-55 0.428063087127299 1106 56-57 0.32139226239377194 1106 58-59 0.2968025568350683 1106 60-61 0.36966617617481035 1106 62-63 0.3164749211951019 1106 64-65 0.33260721167408036 1106 66-67 0.3304637676324589 1106 68-69 0.3248504500699454 1106 70-71 0.28260159075176716 1106 72-73 0.3085734763855328 1106 74-75 0.33847116606323624 1106 76-77 0.28158077686698135 1106 78-79 0.2703568018797142 1106 80-81 0.2379751031719124 1106 82-83 0.2824372581624033 1106 84-85 0.273926776878028 1106 86-87 0.23888721258710177 1106 88-89 0.3109114396025561 1106 90-91 0.28398242772485816 1106 92-93 0.30083415656167745 1106 94-95 0.36407735165133204 1106 96-97 0.2623439842823103 1106 98-99 0.2756630618578626 1106 100 0.29424568754364344 1107 1 0.07012084458337853 1107 2 0.08386971119600872 1107 3 0.0374211184181803 1107 4 0.049599126602949184 1107 5 0.06933818783284096 1107 6 0.06718046485561757 1107 7 0.04578638489327602 1107 8 0.08951069233905429 1107 9 0.06829666096047049 1107 10-11 0.12280840941360793 1107 12-13 0.12684483823977644 1107 14-15 0.17684409825884728 1107 16-17 0.2241134351423213 1107 18-19 0.15598884546261615 1107 20-21 0.1297585153842462 1107 22-23 0.1817426282075374 1107 24-25 0.17922861673032742 1107 26-27 0.19997773349455628 1107 28-29 0.21352462147279283 1107 30-31 0.26329885472228653 1107 32-33 0.17870592294855214 1107 34-35 0.11690937377913002 1107 36-37 0.14758806430596394 1107 38-39 0.1680339197353149 1107 40-41 0.1694833818400383 1107 42-43 0.15851811816571626 1107 44-45 0.15026875658753625 1107 46-47 0.29538344028160424 1107 48-49 0.314736983513491 1107 50-51 0.29737376969187324 1107 52-53 0.2180624294513933 1107 54-55 0.21013305392373383 1107 56-57 0.18153355917041836 1107 58-59 0.24337750416660242 1107 60-61 0.17224682409307945 1107 62-63 0.14282464407137496 1107 64-65 0.21538398285149896 1107 66-67 0.20903550143984972 1107 68-69 0.18342942712658328 1107 70-71 0.1553288343428747 1107 72-73 0.18288611553498413 1107 74-75 0.17399460299569114 1107 76-77 0.14274253849357166 1107 78-79 0.1899007549417533 1107 80-81 0.18645284567119091 1107 82-83 0.14478325899141709 1107 84-85 0.10491773456805475 1107 86-87 0.16591671120042406 1107 88-89 0.23231876660855022 1107 90-91 0.1784867458979349 1107 92-93 0.1768283405957618 1107 94-95 0.2068257694607425 1107 96-97 0.18191827779843095 1107 98-99 0.21462949933302156 1107 100 0.10078491005882384 1108 1 0.055832091232602465 1108 2 0.02052288543467995 1108 3 0.04800581686694727 1108 4 0.011471776112877308 1108 5 0.030246811745364255 1108 6 0.0743853224595199 1108 7 -0.0020155641840133853 1108 8 0.02593365150968907 1108 9 0.07564115653633507 1108 10-11 0.04586223867681838 1108 12-13 0.012263533649246483 1108 14-15 0.10187036025711649 1108 16-17 0.1577196403948804 1108 18-19 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-0.2509522466880867 2214 96-97 -0.2682811373558849 2214 98-99 -0.24962398697265087 2214 100 -0.49546830945461906 2215 1 -0.46798246603118443 2215 2 -0.4714480872456477 2215 3 -0.48614562854582744 2215 4 -0.30962171467446353 2215 5 -0.2942339228753852 2215 6 -0.33924683905290465 2215 7 -0.34948507978327115 2215 8 -0.29094285035274225 2215 9 -0.37504351545770476 2215 10-11 -0.3976114431106481 2215 12-13 -0.43938377229861914 2215 14-15 -0.43155843168587893 2215 16-17 -0.3748907837011686 2215 18-19 -0.42375306518747635 2215 20-21 -0.5275311057026215 2215 22-23 -0.4643550436688102 2215 24-25 -0.4735276194731739 2215 26-27 -0.5251508296005696 2215 28-29 -0.5541783890988228 2215 30-31 -0.5069098173902589 2215 32-33 -0.5287620358268583 2215 34-35 -0.511140336830529 2215 36-37 -0.5721679397862687 2215 38-39 -0.5147131851701303 2215 40-41 -0.5526967385447037 2215 42-43 -0.5213415838469757 2215 44-45 -0.5910048978307714 2215 46-47 -0.5412428496886648 2215 48-49 -0.4485190202384004 2215 50-51 -0.43151337930790135 2215 52-53 -0.46975040174109495 2215 54-55 -0.4528309899310017 2215 56-57 -0.4532761027843648 2215 58-59 -0.5537473111143498 2215 60-61 -0.5144510732238956 2215 62-63 -0.500434820929172 2215 64-65 -0.4883248624936485 2215 66-67 -0.4818470352844457 2215 68-69 -0.43868014096267416 2215 70-71 -0.4208526358267406 2215 72-73 -0.4184039228076273 2215 74-75 -0.3936978230299104 2215 76-77 -0.4777714348088651 2215 78-79 -0.440964512976592 2215 80-81 -0.3344551211906648 2215 82-83 -0.39474678716536715 2215 84-85 -0.45615737622934205 2215 86-87 -0.3906016844682938 2215 88-89 -0.31353889813429703 2215 90-91 -0.3637026419205114 2215 92-93 -0.3401106427296625 2215 94-95 -0.3577776367891019 2215 96-97 -0.3495085496353454 2215 98-99 -0.35506920867168645 2215 100 -0.5048410654381215 2216 1 -0.5194021837209348 2216 2 -0.5217659622337543 2216 3 -0.4520584468998905 2216 4 -0.33144072737703567 2216 5 -0.28383286090411985 2216 6 -0.34261131798761113 2216 7 -0.35726376970055185 2216 8 -0.37068429065570285 2216 9 -0.45890545689598383 2216 10-11 -0.4351591402844619 2216 12-13 -0.41364046969247426 2216 14-15 -0.4986045053360968 2216 16-17 -0.49396669524337966 2216 18-19 -0.5113949740372803 2216 20-21 -0.5310603754423013 2216 22-23 -0.5373103505854218 2216 24-25 -0.5049544686990899 2216 26-27 -0.5704331341070414 2216 28-29 -0.572903162033704 2216 30-31 -0.5770939784249194 2216 32-33 -0.6107623006617615 2216 34-35 -0.6880040915942445 2216 36-37 -0.6869397227966729 2216 38-39 -0.6634209479971034 2216 40-41 -0.6386537971729567 2216 42-43 -0.6231281673273585 2216 44-45 -0.616825922589392 2216 46-47 -0.648828440393217 2216 48-49 -0.5064307437465843 2216 50-51 -0.5230872406248466 2216 52-53 -0.5963464210510665 2216 54-55 -0.5963547609001836 2216 56-57 -0.6354374433210097 2216 58-59 -0.6744538083521192 2216 60-61 -0.6883442074311859 2216 62-63 -0.670960656587205 2216 64-65 -0.5906766873116283 2216 66-67 -0.6745230578150441 2216 68-69 -0.557505106811405 2216 70-71 -0.5989099621408371 2216 72-73 -0.5793759384466668 2216 74-75 -0.5325370002580314 2216 76-77 -0.578090160902839 2216 78-79 -0.5382236048497617 2216 80-81 -0.5645218170838575 2216 82-83 -0.5535492007971854 2216 84-85 -0.5145105782723718 2216 86-87 -0.49937937164709467 2216 88-89 -0.5309626904803366 2216 90-91 -0.5812611752906989 2216 92-93 -0.6162991703029306 2216 94-95 -0.5848911389101303 2216 96-97 -0.6095694694959519 2216 98-99 -0.6590694871869474 2216 100 -0.790057319951984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 15.0 8 95.0 9 282.0 10 342.0 11 200.0 12 204.0 13 251.0 14 426.0 15 779.0 16 1313.0 17 2666.0 18 4370.0 19 6508.0 20 9190.0 21 13422.0 22 19590.0 23 23142.0 24 22503.0 25 20384.0 26 19550.0 27 22297.0 28 27408.0 29 33447.0 30 41905.0 31 53036.0 32 67325.0 33 88071.0 34 127647.0 35 200847.0 36 375525.0 37 824898.0 38 980820.0 39 297693.0 40 628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03789333918318 19.48444320578185 13.540424602313749 22.937238852721226 2 14.892250435017552 22.447392465699867 37.462959993598346 25.19739710568424 3 20.69637909280623 25.568452704217787 30.7455063066418 22.989661896334184 4 12.218426647249773 17.467471428675797 39.664949018480954 30.64915290559348 5 12.272534900370067 39.20293618578805 35.021463693361724 13.503065220480156 6 29.121465583078006 38.02276509838405 17.38405226053593 15.471717058002014 7 25.33029287022557 33.40999397586696 23.2013703381425 18.058342815764973 8 25.462902394514725 31.80902663472439 20.665901577297046 22.06216939346384 9 24.968472153723845 16.640100621368592 22.470645255215064 35.9207819696925 10-11 25.103543747290274 25.125328019106874 28.006723916300935 21.764404317301913 12-13 27.5562556666403 23.574182026177596 27.263902664613997 21.605659642568106 14-15 23.446792909777958 24.947273623424753 27.778783490224473 23.827149976572816 16-17 21.315627420686667 27.68908053915481 28.279279317653117 22.71601272250541 18-19 21.027085039982172 28.649398993390946 28.983982031031914 21.33953393559497 20-21 22.939587124093848 25.84399994584356 28.062360225808007 23.154052704254582 22-23 22.10587565946002 25.970843804574688 28.432751811803648 23.490528724161642 24-25 23.598080796402556 26.155523034702654 27.974446114434187 22.271950054460596 26-27 22.956903664867944 24.826745114361305 30.16629365676274 22.050057564008014 28-29 21.41209959475529 28.107513753204316 28.67910670612373 21.80127994591666 30-31 22.605269270912647 25.59657670356658 28.410978121857195 23.38717590366358 32-33 22.634625928037643 26.24932532629259 28.74861490324555 22.367433842424216 34-35 23.689923216480118 25.68158798919183 28.795325993002862 21.83316280132519 36-37 23.071877219593592 24.96327330849949 30.141124508390206 21.823724963516707 38-39 22.603849199865763 26.312241748030964 29.298570842236476 21.785338209866794 40-41 22.280388083192328 26.70156821158283 28.712570200009463 22.305473505215378 42-43 22.198260911895535 26.560037483494487 27.24485666822848 23.996844936381503 44-45 23.035196299341052 25.383598954108688 27.756067818639963 23.825136927910293 46-47 24.117677483737886 26.452287649310268 28.025514333177153 21.404520533774697 48-49 23.767595322528113 26.01207475229142 27.983322864613054 22.237007060567414 50-51 21.229531638868437 26.889950042290632 29.271779674940213 22.608738643900715 52-53 22.43274659152088 27.731723564786243 26.44176274767313 23.39376709601975 54-55 21.947666834460655 26.180426154138935 27.288353286822602 24.583553724577804 56-57 23.79591660394052 25.26989990793404 27.10904370060126 23.82513978752418 58-59 24.680782833749372 25.46245129879761 27.121155949879387 22.73560991757363 60-61 23.011939725425563 26.422685691284332 28.76125082490076 21.804123758389345 62-63 21.856988884992802 26.51112907875431 28.212798421551845 23.419083614701048 64-65 21.21888848153123 28.324127219317475 27.169846338268783 23.28713796088251 66-67 22.9246456975605 25.917337544192726 26.442949548793028 24.715067209453746 68-69 21.99579394722718 26.12394164101778 27.28186882129509 24.59839559045995 70-71 23.577429336033536 25.978845726419596 26.704307665111543 23.739417272435322 72-73 23.128306532192404 24.70873307864846 28.946114554644264 23.21684583451487 74-75 22.05705950801893 27.924175420784092 26.896774461491134 23.12199060970584 76-77 22.47219352019872 25.76549209467165 26.922219139036656 24.840095246092975 78-79 22.68885869007144 26.08841627553654 27.484268970147134 23.738456064244883 80-81 23.336182659938423 25.674395141814472 27.252569147893134 23.736853050353975 82-83 22.590406716892453 25.008579883277076 28.827631970470595 23.573381429359873 84-85 22.367292521295294 26.438476322723997 27.390295424763423 23.803935731217287 86-87 22.01945369023786 26.811073451828232 27.053864268372084 24.11560858956182 88-89 21.996422030071987 26.281223568355657 26.129144633957857 25.593209767614507 90-91 22.63472008149014 25.0367989563031 26.752521770560712 25.575959191646042 92-93 23.53595581082078 26.178970131078238 26.889088172229485 23.395985885871497 94-95 22.272528196708294 25.722444383560727 27.40804779638889 24.59697962334209 96-97 20.859597720558483 25.97084675836764 28.72849332323223 24.44106219784165 98-99 22.168410624879822 26.653885144591538 26.268035911178618 24.909668319350025 100 22.073935319764125 24.197617172144987 26.37598428133799 27.352463226752903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 379.0 1 234.0 2 270.0 3 469.0 4 330.5 5 219.0 6 450.0 7 1059.5 8 1922.0 9 2414.5 10 1967.0 11 1444.0 12 1500.0 13 1691.0 14 1929.5 15 2268.0 16 2652.0 17 2913.0 18 3274.5 19 3801.5 20 4397.0 21 5101.5 22 6044.0 23 7213.5 24 8551.0 25 10545.0 26 13244.5 27 16561.0 28 20128.0 29 24945.0 30 31104.0 31 37123.0 32 44012.5 33 50331.0 34 54832.5 35 59790.0 36 67138.0 37 75980.0 38 80401.5 39 80724.5 40 80395.5 41 79923.0 42 80404.5 43 83826.5 44 90968.5 45 100908.5 46 113321.0 47 161189.5 48 246897.0 49 244383.0 50 180422.0 51 147516.0 52 124130.0 53 113709.0 54 106849.5 55 96748.0 56 86916.5 57 75969.0 58 65442.0 59 58772.0 60 50133.5 61 39999.5 62 31229.5 63 24247.5 64 18037.0 65 12898.5 66 9572.0 67 7314.0 68 5637.0 69 4313.5 70 3421.5 71 2733.0 72 2131.0 73 1879.5 74 1538.5 75 1178.5 76 917.5 77 634.5 78 437.5 79 278.0 80 183.0 81 103.5 82 45.0 83 25.0 84 14.5 85 7.5 86 4.5 87 2.5 88 2.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0024644180626631536 2 0.003985663780356458 3 2.1297440047706264E-4 4 0.05074875714224864 5 0.002038469261709028 6 0.005172235440157236 7 0.0 8 5.476484583695897E-4 9 9.127474306159829E-5 10-11 1.5212457176933046E-4 12-13 0.0 14-15 0.0 16-17 1.8254948612319658E-4 18-19 7.606228588466523E-5 20-21 1.6733702894626353E-4 22-23 0.0 24-25 0.0 26-27 1.2169965741546437E-4 28-29 1.5212457176933046E-5 30-31 1.977619433001296E-4 32-33 1.8254948612319658E-4 34-35 2.1297440047706264E-4 36-37 4.5637371530799144E-5 38-39 5.780733727234557E-4 40-41 3.19461600715594E-4 42-43 0.0 44-45 4.259488009541253E-4 46-47 0.0 48-49 1.369121145923974E-4 50-51 0.0 52-53 4.1073634377719224E-4 54-55 3.498865150694601E-4 56-57 1.8254948612319658E-4 58-59 8.671100590851836E-4 60-61 6.38923201431188E-4 62-63 2.281868576539957E-4 64-65 4.563737153079914E-4 66-67 0.0012169965741546437 68-69 0.0026773924631402165 70-71 0.0035140776078715335 72-73 0.0031641910928020735 74-75 0.0026926049203171497 76-77 0.0041377883521257885 78-79 0.001323483774393175 80-81 0.0011713592026238446 82-83 6.693481157850541E-4 84-85 6.38923201431188E-4 86-87 0.0 88-89 0.0 90-91 6.0849828707732184E-5 92-93 7.606228588466523E-5 94-95 1.369121145923974E-4 96-97 3.042491435386609E-4 98-99 1.2169965741546437E-4 100 0.07700545822963509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3286780.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.47684619947111 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00485764743813 39.39125567122265 2 15.500296735671487 17.198151561020225 3 5.971303931768563 9.93807329499065 4 2.8659938354131618 6.359851968633932 5 1.5616264809259472 4.331705605167504 6 0.9421742795714447 3.1361314560529525 7 0.5876149733970236 2.281931785255709 8 0.3848355098078364 1.7079568311763518 9 0.2718294469721879 1.357221637994702 >10 0.8863543287851401 7.300916988570553 >50 0.014306092643218667 0.5351748079832018 >100 0.00707886380631261 0.7306875887313531 >500 7.853971816111814E-4 0.31144807162835 >1k 3.9269859080557197E-4 0.27794169682668834 >5k 2.3561915448334315E-4 1.0163455118638238 >10k+ 3.1415887264445755E-4 4.125205522881387 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 57379 1.7457511607104828 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15603 0.47471993866337264 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11610 0.3532332556483853 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10085 0.30683526125873956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9633 0.2930831999707921 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8639 0.26284083510304923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5054 0.15376751714443923 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.084982870773219E-5 2 0.0 0.0 0.0 0.0 6.084982870773219E-5 3 0.0 0.0 0.0 3.0424914353866095E-5 6.084982870773219E-5 4 0.0 0.0 0.0 3.0424914353866095E-5 6.084982870773219E-5 5 0.0 0.0 0.0 3.0424914353866095E-5 6.084982870773219E-5 6 0.0 0.0 0.0 9.127474306159828E-5 9.127474306159828E-5 7 0.0 0.0 0.0 9.127474306159828E-5 9.127474306159828E-5 8 0.0 0.0 0.0 9.127474306159828E-5 9.127474306159828E-5 9 0.0 0.0 0.0 9.127474306159828E-5 9.127474306159828E-5 10-11 1.5212457176933048E-5 0.0 0.0 1.673370289462635E-4 1.5212457176933046E-4 12-13 3.0424914353866095E-5 0.0 0.0 2.586117720078618E-4 6.237107442542549E-4 14-15 3.0424914353866095E-5 0.0 0.0 2.738242291847948E-4 7.454104016697194E-4 16-17 3.0424914353866095E-5 0.0 0.0 3.34674057892527E-4 9.127474306159829E-4 18-19 3.0424914353866095E-5 0.0 0.0 4.8679862966185747E-4 0.001004022173677581 20-21 3.0424914353866095E-5 0.0 0.0 6.69348115785054E-4 0.0010344470880314471 22-23 3.0424914353866095E-5 0.0 0.0 8.823225162621168E-4 0.0011257218310930454 24-25 3.0424914353866095E-5 0.0 0.0 0.0014147585174547734 0.0011561467454469115 26-27 3.0424914353866095E-5 0.0 0.0 0.002920791777971145 0.0013691211459239741 28-29 3.0424914353866095E-5 0.0 0.0 0.007149854873158532 0.0013995460602778402 30-31 3.0424914353866095E-5 0.0 0.0 0.018391860726912054 0.0013995460602778402 32-33 3.0424914353866095E-5 0.0 0.0 0.047934452564516034 0.0014756083461625056 34-35 3.0424914353866095E-5 0.0 0.0 0.09077273197475949 0.0014908208033394385 36-37 3.0424914353866095E-5 0.0 0.0 0.15242882091286913 0.0014908208033394385 38-39 3.0424914353866095E-5 0.0 0.0 0.2452248096921607 0.001582095546401037 40-41 3.0424914353866095E-5 0.0 0.0 0.35426770273641683 0.001582095546401037 42-43 3.0424914353866095E-5 0.0 0.0 0.46670297373112896 0.0016429453751087692 44-45 3.0424914353866095E-5 0.0 0.0 0.6078441514187137 0.0017494325753473006 46-47 3.0424914353866095E-5 0.0 0.0 0.78047511546255 0.0018559197755858318 48-49 3.0424914353866095E-5 0.0 0.0 0.951098035159031 0.001977619433001296 50-51 3.0424914353866095E-5 0.0 0.0 1.1373289359190455 0.0020688941760628942 52-53 3.0424914353866095E-5 0.0 0.0 1.3274846506307085 0.0021601689191244927 54-55 3.0424914353866095E-5 0.0 0.0 1.5492366388988614 0.0021753813763014255 56-57 3.0424914353866095E-5 0.0 0.0 1.8312755949592003 0.002236231205009158 58-59 3.0424914353866095E-5 0.0 0.0 2.111930217416438 0.002281868576539957 60-61 3.0424914353866095E-5 0.0 0.0 2.408390582880509 0.002312293490893823 62-63 3.0424914353866095E-5 0.0 0.0 2.70976457201273 0.0024339931483092873 64-65 3.0424914353866095E-5 0.0 0.0 3.0335008731950417 0.0024339931483092873 66-67 3.0424914353866095E-5 0.0 0.0 3.4082749682059648 0.0024492056054862205 68-69 3.0424914353866095E-5 0.0 0.0 3.836733824594284 0.0024644180626631536 70-71 3.0424914353866095E-5 0.0 0.0 4.298614449400325 0.0024644180626631536 72-73 3.0424914353866095E-5 0.0 0.0 4.739745282617029 0.002586117720078618 74-75 3.0424914353866095E-5 0.0 0.0 5.207071967092412 0.0027686672062018146 76-77 3.0424914353866095E-5 0.0 0.0 5.691512665891846 0.0029055793207942117 78-79 3.0424914353866095E-5 0.0 0.0 6.2924807866665855 0.002936004235148078 80-81 3.0424914353866095E-5 0.0 0.0 6.921561528304298 0.0029664291495019443 82-83 3.0424914353866095E-5 0.0 0.0 7.52951216692325 0.0030729163497404755 84-85 3.0424914353866095E-5 0.0 0.0 8.139288300403434 0.0030729163497404755 86-87 3.0424914353866095E-5 0.0 0.0 8.762786070257212 0.00319461600715594 88 3.0424914353866095E-5 0.0 0.0 9.278686130498542 0.0033163156645714042 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1050 0.0 23.735134 1 GAGTACT 11505 0.0 22.283764 12-13 GTACTTT 12605 0.0 20.32064 14-15 AGTACTT 12345 0.0 19.968012 12-13 CATGGGG 6855 0.0 19.000463 4 GGGCTAT 1890 0.0 18.899574 6 GTATAAG 1450 0.0 18.808975 1 TAGGCAT 1670 0.0 18.5758 5 ACTTTTT 14205 0.0 18.098104 16-17 GTGATCG 820 0.0 17.76758 8 TATCACG 160 0.001275454 17.632877 2 GAGTACG 830 0.0 17.562595 1 TACGCAG 750 0.0 17.554506 4 TACTTTT 14165 0.0 17.41913 14-15 CTATTGA 2110 0.0 17.37371 9 AGGGCTA 2255 0.0 17.30022 5 TGATCGC 820 0.0 17.194431 9 ACGTGCG 885 0.0 16.99367 9 CTAGTAC 1375 0.0 16.756577 3 TAGTACT 1715 0.0 16.724672 4 >>END_MODULE