FastQCFastQC Report
Mon 16 Jan 2017
ERR1146591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632847
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA239640.42543317792938457No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG106970.1899039686325583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21000.027.3213371
TCTAACG10400.023.5056952
CTAACGC10400.022.6016313
CATGGGG119050.021.6791464
TGATCGC17550.019.5466029
CTAGTAC27550.018.7704283
CGACGAG11600.018.63565624-25
TAACGCC13750.018.4624924
ATGGGGG81400.018.0184675
CGCCTAC13050.018.0053047
TAGGCAT31550.017.8800835
GTATAAG32800.017.6356981
GGGCTAT38400.017.5003136
CGTTAAC5650.017.4796311
GTGATCG19750.017.131788
CTATTGA37000.017.0187879
TAGTACT34650.016.9592274
GCATCGT26000.016.8091898
TACCTGG61900.016.7843592
CTGTGCG29650.016.6414139
Produced by FastQC (version 0.11.5)