##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146591_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5632847
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225476566290546	31.0	31.0	34.0	28.0	34.0
2	31.426876142739186	31.0	31.0	34.0	28.0	34.0
3	31.600085001421128	33.0	31.0	34.0	28.0	34.0
4	35.137109351629825	37.0	35.0	37.0	32.0	37.0
5	35.25109789064038	37.0	35.0	37.0	33.0	37.0
6	35.28041166394188	37.0	35.0	37.0	33.0	37.0
7	35.190310157545554	37.0	35.0	37.0	32.0	37.0
8	35.19904659224722	37.0	35.0	37.0	32.0	37.0
9	36.85410876595796	39.0	37.0	39.0	33.0	39.0
10-11	36.85406172047634	39.0	37.0	39.0	33.0	39.0
12-13	36.81752859610779	39.0	37.0	39.0	33.0	39.0
14-15	38.18500910818277	40.0	38.0	41.0	33.0	41.0
16-17	38.19914973724654	40.0	38.0	41.0	33.0	41.0
18-19	38.20742255204162	40.0	38.0	41.0	33.0	41.0
20-21	38.289828482825826	40.0	38.5	41.0	33.0	41.0
22-23	38.29985511766962	40.0	38.0	41.0	33.0	41.0
24-25	38.311272878528385	40.0	38.0	41.0	33.0	41.0
26-27	38.35530185712483	40.0	38.0	41.0	33.0	41.0
28-29	38.326673350083894	40.0	38.0	41.0	33.0	41.0
30-31	38.28808203027705	40.0	38.0	41.0	33.0	41.0
32-33	38.23663113874743	40.0	38.0	41.0	33.0	41.0
34-35	38.1974679056612	40.0	38.0	41.0	33.0	41.0
36-37	38.138401060777966	40.0	38.0	41.0	33.0	41.0
38-39	38.07345557228875	40.0	38.0	41.0	33.0	41.0
40-41	38.005624420475115	40.0	38.0	41.0	32.0	41.0
42-43	37.859491124115394	40.0	38.0	41.0	32.0	41.0
44-45	37.744882383633	40.0	37.5	41.0	32.0	41.0
46-47	37.618261422687326	40.0	37.0	41.0	31.0	41.0
48-49	37.50991195038672	40.0	37.0	41.0	31.0	41.0
50-51	37.37693541116952	40.0	36.5	41.0	31.0	41.0
52-53	37.2570494991254	40.0	36.0	41.0	31.0	41.0
54-55	37.130932990013754	39.5	36.0	41.0	31.0	41.0
56-57	36.92184351891681	39.0	35.0	41.0	30.0	41.0
58-59	36.72073518062891	39.0	35.0	41.0	30.0	41.0
60-61	36.487224133728475	39.0	35.0	41.0	30.0	41.0
62-63	36.21811874173042	38.0	35.0	40.0	30.0	41.0
64-65	35.930819708044616	37.5	35.0	40.0	29.0	41.0
66-67	35.66276618200352	37.0	35.0	40.0	29.0	41.0
68-69	35.38717437203603	36.5	34.5	39.5	29.0	41.0
70-71	35.05834039163499	36.0	34.0	39.0	29.0	41.0
72-73	34.7031031554736	35.5	34.0	39.0	28.5	41.0
74-75	34.37272297649838	35.0	34.0	37.5	28.0	40.0
76-77	34.0689056528608	35.0	34.0	37.0	28.0	39.0
78-79	33.74747210424853	35.0	34.0	37.0	27.5	39.0
80-81	33.50731175549416	35.0	34.0	36.0	27.0	39.0
82-83	33.243168330331	35.0	33.0	36.0	27.0	37.0
84-85	33.038607830107935	35.0	33.0	35.5	27.0	37.0
86-87	32.832179180439304	35.0	33.0	35.0	26.0	37.0
88-89	32.64708920728719	35.0	33.0	35.0	26.0	36.0
90-91	32.489541345610846	35.0	33.0	35.0	26.0	36.0
92-93	32.30981180564642	35.0	33.0	35.0	25.0	36.0
94-95	32.186126749048924	35.0	33.0	35.0	25.0	35.5
96-97	32.092122420509554	35.0	33.0	35.0	25.0	35.0
98-99	31.981658564487905	35.0	33.0	35.0	25.0	35.0
100	30.77671806104444	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2882787279326138
1101	2	0.27985414903089634
1101	3	0.31228952101547236
1101	4	0.3595111636578494
1101	5	0.3618710783738379
1101	6	0.36740747342893343
1101	7	0.3674732948725037
1101	8	0.4210370549528477
1101	9	0.5375692577658953
1101	10-11	0.5442131251894295
1101	12-13	0.5124813772125876
1101	14-15	0.6134095311447325
1101	16-17	0.610810562043433
1101	18-19	0.606652219814265
1101	20-21	0.6166988680684824
1101	22-23	0.5818272849810597
1101	24-25	0.6305823100263837
1101	26-27	0.5590788128309612
1101	28-29	0.6001658854309966
1101	30-31	0.6026367629129368
1101	32-33	0.6220149736736218
1101	34-35	0.624907629624964
1101	36-37	0.6491372764336916
1101	38-39	0.6488330923552894
1101	40-41	0.661911289207886
1101	42-43	0.6510308466821115
1101	44-45	0.6355775105138832
1101	46-47	0.6068912954509358
1101	48-49	0.6497681746692692
1101	50-51	0.6304748012139356
1101	52-53	0.626661462005984
1101	54-55	0.6134651707432326
1101	56-57	0.6082266100414486
1101	58-59	0.665363461787436
1101	60-61	0.6374858454773573
1101	62-63	0.626713209282336
1101	64-65	0.6534476918406327
1101	66-67	0.6127199496205478
1101	68-69	0.5803131477041674
1101	70-71	0.5501018917830933
1101	72-73	0.6215285173173939
1101	74-75	0.6192520947113636
1101	76-77	0.586708137021624
1101	78-79	0.5890523074572371
1101	80-81	0.6025705502384255
1101	82-83	0.5423751536895409
1101	84-85	0.47871319343136776
1101	86-87	0.49503086696645937
1101	88-89	0.448670520949058
1101	90-91	0.40774327286149514
1101	92-93	0.456810065190254
1101	94-95	0.48617216076172554
1101	96-97	0.45651390747622145
1101	98-99	0.4443999832327563
1101	100	0.4416380742205419
1102	1	0.3091088359340013
1102	2	0.3004149426056806
1102	3	0.34887626930306226
1102	4	0.29428713118340255
1102	5	0.31858998358601553
1102	6	0.37483298164317347
1102	7	0.32640859631446517
1102	8	0.34032463363544707
1102	9	0.3857752911227692
1102	10-11	0.40609407879787085
1102	12-13	0.40939877482583853
1102	14-15	0.5501428971065963
1102	16-17	0.48745384889732435
1102	18-19	0.4283019297450892
1102	20-21	0.48836013224877206
1102	22-23	0.5347480652351422
1102	24-25	0.552110287482634
1102	26-27	0.5033846919109877
1102	28-29	0.5156942709210028
1102	30-31	0.5087049951313745
1102	32-33	0.4946956483856084
1102	34-35	0.521921958033218
1102	36-37	0.5178890222889407
1102	38-39	0.5417190496541835
1102	40-41	0.5196019719081946
1102	42-43	0.5710375810613257
1102	44-45	0.4979836728106406
1102	46-47	0.4827237632753736
1102	48-49	0.5049630768440494
1102	50-51	0.4925551116567135
1102	52-53	0.4696244248564625
1102	54-55	0.42139698552384885
1102	56-57	0.42859355531352605
1102	58-59	0.439307788337743
1102	60-61	0.44427978402735846
1102	62-63	0.3733873716267695
1102	64-65	0.4904591532309226
1102	66-67	0.45476143877215236
1102	68-69	0.3551415252445693
1102	70-71	0.36954209589412557
1102	72-73	0.3972774384462312
1102	74-75	0.48396362114863223
1102	76-77	0.37504533417291697
1102	78-79	0.3421365441808888
1102	80-81	0.35127334988602854
1102	82-83	0.37412010199400214
1102	84-85	0.3734709979880577
1102	86-87	0.32876129734792414
1102	88-89	0.358344041555263
1102	90-91	0.3564537089056188
1102	92-93	0.3767358704703838
1102	94-95	0.39663075152368066
1102	96-97	0.4089066127832979
1102	98-99	0.4334108517820354
1102	100	0.4724089368444453
1103	1	0.05321268532855683
1103	2	0.047339470816904594
1103	3	0.10270779168921962
1103	4	0.1145337147998049
1103	5	0.14234105235717465
1103	6	0.22199340535731693
1103	7	0.22379945590540018
1103	8	0.2131291882908073
1103	9	0.2921324411975732
1103	10-11	0.3008429544837483
1103	12-13	0.2783020680298023
1103	14-15	0.37922939510734466
1103	16-17	0.38500947076980907
1103	18-19	0.3801065777669308
1103	20-21	0.30676073169482265
1103	22-23	0.3143197108111053
1103	24-25	0.36423456754300076
1103	26-27	0.35337594025445895
1103	28-29	0.3700265606110378
1103	30-31	0.3894465582942246
1103	32-33	0.36473943453352575
1103	34-35	0.36263211883416346
1103	36-37	0.33813198878023343
1103	38-39	0.3766970495847701
1103	40-41	0.3261113395388051
1103	42-43	0.39076831798116984
1103	44-45	0.310928531194115
1103	46-47	0.3654012921776797
1103	48-49	0.3251799464475056
1103	50-51	0.33180789256054766
1103	52-53	0.2861976616280941
1103	54-55	0.22555339987362544
1103	56-57	0.2561526351576191
1103	58-59	0.2445233237132598
1103	60-61	0.21857210600321508
1103	62-63	0.2522987677268489
1103	64-65	0.21318632909555646
1103	66-67	0.2225399257446199
1103	68-69	0.22552812554119583
1103	70-71	0.2825709930441107
1103	72-73	0.28084149345389875
1103	74-75	0.32004397791398986
1103	76-77	0.2191782777570026
1103	78-79	0.24111318714476226
1103	80-81	0.21118275528120733
1103	82-83	0.2133978812513746
1103	84-85	0.1857249586820089
1103	86-87	0.1139930292272453
1103	88-89	0.12886630994390913
1103	90-91	0.1743561721713931
1103	92-93	0.14642465499077417
1103	94-95	0.2145835866057979
1103	96-97	0.20174483724678538
1103	98-99	0.20090570255883478
1103	100	0.2739805267970681
1104	1	0.1608620814020334
1104	2	0.13246416520839333
1104	3	0.20395209725720065
1104	4	0.19570394782319056
1104	5	0.1702145881065249
1104	6	0.23201224085983085
1104	7	0.21842202062838822
1104	8	0.2869323920851343
1104	9	0.3204542155126191
1104	10-11	0.38752218325034704
1104	12-13	0.3882051476561301
1104	14-15	0.4613816284870964
1104	16-17	0.46097076583870233
1104	18-19	0.4084138475469459
1104	20-21	0.42555929735181053
1104	22-23	0.4037585994323365
1104	24-25	0.48886811135778885
1104	26-27	0.383511724624789
1104	28-29	0.34575504387257183
1104	30-31	0.3841493695484175
1104	32-33	0.4216887509977525
1104	34-35	0.41211221155102606
1104	36-37	0.46653260760641047
1104	38-39	0.40861760551887016
1104	40-41	0.41744458671163187
1104	42-43	0.4501256166810421
1104	44-45	0.45807584093426357
1104	46-47	0.4120429824938725
1104	48-49	0.3964515353262783
1104	50-51	0.4102940983398753
1104	52-53	0.37269768673700554
1104	54-55	0.36925000680658826
1104	56-57	0.41481509270509065
1104	58-59	0.38571658555376587
1104	60-61	0.40438972970153486
1104	62-63	0.3905207411542406
1104	64-65	0.41441735370968047
1104	66-67	0.39714597722016975
1104	68-69	0.36654863301637874
1104	70-71	0.36974243282441677
1104	72-73	0.46400978353236866
1104	74-75	0.4014155665123482
1104	76-77	0.3469897677449225
1104	78-79	0.38011093652016115
1104	80-81	0.33599053195521833
1104	82-83	0.32543498838941787
1104	84-85	0.28094157205916304
1104	86-87	0.24575523336596206
1104	88-89	0.28713882746979635
1104	90-91	0.28597333605810604
1104	92-93	0.2857146873162577
1104	94-95	0.28826308124638444
1104	96-97	0.31342985890545094
1104	98-99	0.37119269097127017
1104	100	0.34941659965215166
1105	1	0.07636009191344684
1105	2	0.09929765619106945
1105	3	0.16155469129885702
1105	4	0.19134102957001886
1105	5	0.2018473231490674
1105	6	0.22370670551106286
1105	7	0.1844475799680083
1105	8	0.24691392563094894
1105	9	0.28571834915847916
1105	10-11	0.27319674888939716
1105	12-13	0.2900233458030286
1105	14-15	0.3917174397913925
1105	16-17	0.3282515731781004
1105	18-19	0.3785620039477209
1105	20-21	0.37496390712053085
1105	22-23	0.3904444752891294
1105	24-25	0.43121762783911777
1105	26-27	0.36983203362235173
1105	28-29	0.3682640689636969
1105	30-31	0.45250284777350913
1105	32-33	0.4167005753052635
1105	34-35	0.36742535848676994
1105	36-37	0.38916449966794175
1105	38-39	0.33567219879449084
1105	40-41	0.3327698747099177
1105	42-43	0.31313605648993104
1105	44-45	0.27524037126422485
1105	46-47	0.33480266882629195
1105	48-49	0.3467188595812445
1105	50-51	0.3940804399115052
1105	52-53	0.317444274217344
1105	54-55	0.2789668132459653
1105	56-57	0.3630833893130472
1105	58-59	0.30825849976873343
1105	60-61	0.34052863879720974
1105	62-63	0.22271897494761106
1105	64-65	0.3237307040482662
1105	66-67	0.30356950224005175
1105	68-69	0.2630978478886732
1105	70-71	0.27314176042994376
1105	72-73	0.21501406688563662
1105	74-75	0.22961127888713406
1105	76-77	0.27123596965792274
1105	78-79	0.2476754398591936
1105	80-81	0.2573995068752595
1105	82-83	0.2633299339743047
1105	84-85	0.27077473075246417
1105	86-87	0.2144514138342899
1105	88-89	0.23280805803177884
1105	90-91	0.23694521573585803
1105	92-93	0.2489267580858865
1105	94-95	0.29380282332283514
1105	96-97	0.3033921953973504
1105	98-99	0.30479899189631254
1105	100	0.41279818897597664
1106	1	0.07362999323538943
1106	2	0.08847016927889939
1106	3	0.07409803111789515
1106	4	0.10120600814175162
1106	5	0.09864789140995356
1106	6	0.13393334290467607
1106	7	0.13083296335243233
1106	8	0.2098259186601652
1106	9	0.2863763888944746
1106	10-11	0.2607676659941305
1106	12-13	0.28031365728981683
1106	14-15	0.2473749713020439
1106	16-17	0.31226374643289745
1106	18-19	0.29834323192443435
1106	20-21	0.31796426274102885
1106	22-23	0.2979115771906393
1106	24-25	0.33509725812188407
1106	26-27	0.328408169208636
1106	28-29	0.319970745452693
1106	30-31	0.3602569071490578
1106	32-33	0.3293902986595967
1106	34-35	0.32812647981209864
1106	36-37	0.29810625558053516
1106	38-39	0.2753489391186008
1106	40-41	0.23436672237708223
1106	42-43	0.31698306154226685
1106	44-45	0.2875591404421769
1106	46-47	0.2501907439294655
1106	48-49	0.2587056446246123
1106	50-51	0.2848723071350605
1106	52-53	0.24529912597610348
1106	54-55	0.19797680584353117
1106	56-57	0.1786936334524185
1106	58-59	0.1714031602783308
1106	60-61	0.2119357807218165
1106	62-63	0.22376145444317075
1106	64-65	0.3155374117261047
1106	66-67	0.3076924402564174
1106	68-69	0.18861390245715626
1106	70-71	0.24308311369632207
1106	72-73	0.19830061238150165
1106	74-75	0.17735789191521434
1106	76-77	0.21184320607309814
1106	78-79	0.1862796463181695
1106	80-81	0.24329364632807682
1106	82-83	0.20272648706290397
1106	84-85	0.19974795272086254
1106	86-87	0.13809799055010075
1106	88-89	0.1704175197511617
1106	90-91	0.19501863824055476
1106	92-93	0.23174615712474633
1106	94-95	0.18416953786606882
1106	96-97	0.19962484128615188
1106	98-99	0.3159597300755479
1106	100	0.3467375409964504
1107	1	0.05387028324836862
1107	2	0.047825769778867766
1107	3	0.06086972325791251
1107	4	0.036494953989901546
1107	5	0.07207686083936693
1107	6	0.11160815587447814
1107	7	0.07038298523416842
1107	8	0.09253024415766475
1107	9	0.1215858005899122
1107	10-11	0.10990317065947153
1107	12-13	0.1497018313640055
1107	14-15	0.12358815808853052
1107	16-17	0.18593858370622485
1107	18-19	0.2321225777932483
1107	20-21	0.24323801546084667
1107	22-23	0.2648255750474817
1107	24-25	0.325295833353195
1107	26-27	0.26424167668873366
1107	28-29	0.23809814831432163
1107	30-31	0.26022118780825565
1107	32-33	0.23303463842771777
1107	34-35	0.25854214032123934
1107	36-37	0.27364026143700215
1107	38-39	0.25225228621081897
1107	40-41	0.16575088705368302
1107	42-43	0.21854850829161165
1107	44-45	0.21996974410588876
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2216	56-57	-0.6134181834219561
2216	58-59	-0.5583995354414242
2216	60-61	-0.6007201009929588
2216	62-63	-0.604850899470712
2216	64-65	-0.6054493980789886
2216	66-67	-0.5539280603901275
2216	68-69	-0.6641529719278694
2216	70-71	-0.610493691063958
2216	72-73	-0.5847048981597638
2216	74-75	-0.5539957180109241
2216	76-77	-0.5638209057410677
2216	78-79	-0.5270405668933904
2216	80-81	-0.5694351587494495
2216	82-83	-0.5183473395897167
2216	84-85	-0.5129884342233666
2216	86-87	-0.5205411466176955
2216	88-89	-0.5349267499460879
2216	90-91	-0.5553651416568428
2216	92-93	-0.48158438680264837
2216	94-95	-0.5571341736886275
2216	96-97	-0.5147252890926595
2216	98-99	-0.5465288548777494
2216	100	-0.5307536204396293
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1677.0
1	421.0
2	318.0
3	313.0
4	212.0
5	330.0
6	464.0
7	1162.0
8	2969.0
9	3741.0
10	3866.0
11	4617.0
12	6015.0
13	7886.0
14	10713.0
15	13335.0
16	14111.0
17	15345.0
18	16769.0
19	18680.0
20	20870.0
21	23931.0
22	26533.0
23	30515.0
24	38015.0
25	45654.0
26	49705.0
27	48565.0
28	53997.0
29	66169.0
30	78791.0
31	93404.0
32	118790.0
33	160849.0
34	239959.0
35	371208.0
36	673174.0
37	1338695.0
38	1538093.0
39	492200.0
40	786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13692630746877	19.70300277800683	13.02139164778172	24.13867926674268
2	14.747163091526305	20.8125166656215	39.89056515753931	24.54975508531288
3	18.398544302403018	26.52442426314282	30.04265033336342	25.03438110109074
4	12.434821544382473	17.617896912052394	38.92063091572332	31.02665062784181
5	13.142663010403933	38.53992443810547	35.09591115373947	13.221501397751126
6	30.03098296825223	37.544903625128114	18.81586279641368	13.608250610205971
7	26.529334334078207	32.782017439116615	23.077990578214465	17.610657648590713
8	25.585431010065506	35.062993382416444	21.03459403020825	18.3169815773098
9	26.54418143633307	15.999475535377645	20.633330117611905	36.823012910677384
10-11	24.3829092740465	26.499594405883908	28.360858741932425	20.756637578137166
12-13	25.397858345214143	24.229213024032546	27.870443653870925	22.502484976882386
14-15	23.441870514053072	25.3593510333813	25.402366019688817	25.79641243287681
16-17	22.461417230498068	28.167109677052537	26.80409760916534	22.567375483284057
18-19	22.516900819240103	28.250604300288717	27.608854228529584	21.6236406519416
20-21	23.348349308682405	27.502259858097965	26.716737091804248	22.432653741415383
22-23	23.154272538370453	27.272536682602667	25.92301808055022	23.65017269847666
24-25	23.05970110802138	27.654499450191274	26.20042460264793	23.085374839139416
26-27	23.330930455565195	27.10329753889353	26.375709703939698	23.19006230160158
28-29	22.932274852128305	27.414364120614266	26.71202464735554	22.941336379901887
30-31	23.321171897363474	27.3217323196454	26.48399518186094	22.87310060113019
32-33	22.84275572241633	27.80883021753174	26.28050112605259	23.06791293399934
34-35	22.97478424471078	27.720814219449892	26.782024609820898	22.52237692601843
36-37	23.45097189077579	27.14260202676409	26.583047396000847	22.823378686459275
38-39	23.549991999275715	27.120811647490843	26.522236638235146	22.8069597149983
40-41	23.118269013699475	27.11098842745005	26.89151715039695	22.87922540845352
42-43	23.288579566419987	26.93903052627398	26.577670905703805	23.19471900160222
44-45	23.225833391823205	27.277990957317794	26.60024290325361	22.895932747605396
46-47	22.908539051289175	27.294635045341696	26.927422008652556	22.869403894716566
48-49	23.15756683048984	27.12323735488215	26.88822689091907	22.830968923708937
50-51	22.591443004077657	27.232065789830244	26.7104240826602	23.4660671234319
52-53	22.39390287559319	27.543771591694323	26.207244546837977	23.85508098587451
54-55	22.708159326568612	27.38607419327125	26.222417049581498	23.683349430578645
56-57	23.207716456012918	26.75897332374691	26.47429488776526	23.559015332474914
58-59	22.897536486216318	26.872692538374395	26.943750138836442	23.286020836572852
60-61	23.036755187461576	27.158655658677826	26.715357146696878	23.08923200716372
62-63	22.64472228513627	27.12389568069395	26.635577005865564	23.595805028304216
64-65	22.134997791917797	27.297147442134474	26.71424039750811	23.85361436843962
66-67	23.164739830461638	26.767470059590252	26.44533152820358	23.622458581744528
68-69	23.12139740148636	26.680014568779324	26.437305244982696	23.761282784751618
70-71	22.737832220127558	26.936914074162537	26.588122037493566	23.737131668216342
72-73	23.168407832929073	26.77559596152025	26.81016042353106	23.24583578201962
74-75	23.092532009538726	27.312576045785324	26.439137469631667	23.155754475044276
76-77	22.90036934402805	27.028355530968362	26.635056165758137	23.43621895924545
78-79	22.95593968943307	27.109337872609053	26.567390152240854	23.367332285717026
80-81	22.78664463247006	27.077270685982306	26.847024112801627	23.289060568746002
82-83	22.86902562168581	26.77532546722739	26.865937164160457	23.489711746926336
84-85	23.01806434815911	27.099427889000605	26.32462334285741	23.557884419982877
86-87	22.858005507021765	26.98478873830131	26.640468817459972	23.516736937216947
88-89	23.17023954672022	26.563680360559584	26.56296064882812	23.703119443892074
90-91	23.300321640318533	26.462319906433844	26.60220176876852	23.635156684479103
92-93	23.245299869908028	26.733776308263067	26.57991736023287	23.441006461596036
94-95	22.626691539468396	26.718576023808517	27.009963099499856	23.644769337223234
96-97	23.10609631310943	26.51287023521607	26.761037522641534	23.61999592903297
98-99	23.063475930592357	26.86509007756173	26.440458137228262	23.630975854617652
100	23.198427718736355	26.770302492933595	26.138517509479716	23.89275227885034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3320.0
1	2138.0
2	965.5
3	976.0
4	915.0
5	779.5
6	663.5
7	654.5
8	735.0
9	776.5
10	708.5
11	663.0
12	698.5
13	731.5
14	786.5
15	968.0
16	1173.0
17	1381.0
18	1728.5
19	2135.5
20	2712.5
21	3874.5
22	5749.5
23	8209.5
24	11281.0
25	15531.5
26	21403.5
27	28324.0
28	35557.0
29	45083.5
30	55593.5
31	67536.0
32	82409.0
33	93830.5
34	104729.5
35	116865.0
36	128180.0
37	141052.5
38	148935.0
39	152572.0
40	155842.0
41	155830.5
42	157068.0
43	169649.0
44	191142.0
45	210580.5
46	218910.5
47	227989.0
48	250757.5
49	262835.0
50	281728.0
51	275455.5
52	230766.5
53	208961.5
54	198076.5
55	179377.0
56	162176.0
57	145101.5
58	126992.0
59	113050.5
60	95069.5
61	75328.0
62	59204.5
63	45777.5
64	33862.5
65	25187.0
66	18935.5
67	13908.5
68	10757.5
69	8439.0
70	6822.0
71	5688.5
72	4688.5
73	4064.0
74	3303.0
75	2487.5
76	1856.0
77	1342.0
78	963.0
79	621.5
80	423.5
81	286.0
82	193.5
83	133.5
84	112.0
85	93.5
86	62.0
87	50.0
88	38.0
89	26.5
90	19.5
91	21.0
92	22.5
93	14.5
94	11.0
95	15.0
96	17.0
97	13.0
98	6.0
99	8.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10280769209602178
2	0.06646727667199198
3	0.0844155717348616
4	0.0858713187132546
5	0.08659919220245109
6	0.09300802950976655
7	0.08945742712344219
8	0.09077115000638221
9	0.0754503007093926
10-11	0.08314623138175065
12-13	0.07140261398898283
14-15	0.0812377825991013
16-17	0.08320836692351133
18-19	0.08243611090448578
20-21	0.07411882481452098
22-23	0.07803336394544357
24-25	0.08035013200252021
26-27	0.07419871336821326
28-29	0.08267577656556267
30-31	0.08784190303766462
32-33	0.08879168917600638
34-35	0.09187183674614276
36-37	0.09155228253137357
38-39	0.09287488192027939
40-41	0.08882719519986962
42-43	0.09498749034014238
44-45	0.09039833675581815
46-47	0.0872560536439211
48-49	0.0965408788841593
50-51	0.09283937589641615
52-53	0.09832505658328727
54-55	0.09005215302315152
56-57	0.09666514996768065
58-59	0.10158273427273987
60-61	0.09942574332304784
62-63	0.10086373728950919
64-65	0.10282544510795341
66-67	0.10509783063520099
68-69	0.10199993005313299
70-71	0.10394388485964559
72-73	0.10083710777161177
74-75	0.10459186979514977
76-77	0.10134306861166299
78-79	0.10838213784255103
80-81	0.10360657763294476
82-83	0.10811584266357668
84-85	0.10437883365197032
86-87	0.10061519512246649
88-89	0.0993103487454923
90-91	0.09939023729918459
92-93	0.09391343311827927
94-95	0.09375365601089468
96-97	0.09216476144301451
98-99	0.10605649327950857
100	0.24863093210236317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5632847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.31936819041414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.58018161121588	36.40391140473086
2	14.82807842084102	15.51591388912632
3	6.075780084857417	9.536429259109225
4	3.1963075293738994	6.689151619164238
5	1.8980687212393175	4.965287813861419
6	1.1387007668724332	3.5745662808423457
7	0.7983258906779638	2.923753434722448
8	0.5438400268065943	2.276269327934312
9	0.3671460449244574	1.7287964193651313
>10	1.5346943800949344	12.668032824481523
>50	0.029241816699283933	1.0327406883760382
>100	0.008817557621517213	0.7373665651390775
>500	3.142879905740373E-4	0.11885252726022326
>1k	2.514303923576065E-4	0.3387987646508211
>5k	1.2571519617880325E-4	0.35024333561747667
>10k+	1.2571519617880325E-4	1.1398858456185312
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	23964	0.42543317792938457	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	10697	0.1899039686325583	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.101204772648715E-5	0.0
4	0.0	0.0	0.0	8.876505965810895E-5	0.0
5	0.0	0.0	0.0	1.0651807158973074E-4	0.0
6	0.0	0.0	0.0	1.775301193162179E-4	0.0
7	0.0	0.0	0.0	1.775301193162179E-4	0.0
8	0.0	0.0	0.0	1.9528313124783967E-4	0.0
9	0.0	0.0	0.0	4.4382529829054473E-4	0.0
10-11	0.0	0.0	0.0	7.278734891964934E-4	0.0
12-13	0.0	0.0	0.0	8.876505965810895E-4	0.0
14-15	0.0	0.0	0.0	8.876505965810895E-4	0.0
16-17	0.0	0.0	0.0	0.0011273162576579837	0.0
18-19	0.0	1.775301193162179E-5	0.0	0.0015533885440169066	0.0
20-21	0.0	1.775301193162179E-5	0.0	0.002006090348273262	0.0
22-23	0.0	1.775301193162179E-5	0.0	0.002512051188324483	0.0
24-25	0.0	1.775301193162179E-5	0.0	0.0036748734698457105	0.0
26-27	0.0	1.775301193162179E-5	0.0	0.005885123455332623	0.0
28-29	0.0	1.775301193162179E-5	0.0	0.013279252924853099	0.0
30-31	0.0	1.775301193162179E-5	0.0	0.03232823472748328	0.0
32-33	0.0	1.775301193162179E-5	0.0	0.07254768325857244	0.0
34-35	0.0	1.775301193162179E-5	0.0	0.13395535153005222	0.0
36-37	0.0	1.775301193162179E-5	0.0	0.23116196836164732	0.0
38-39	0.0	3.550602386324358E-5	0.0	0.369085118058417	0.0
40-41	0.0	3.550602386324358E-5	0.0	0.5250719573956119	0.0
42-43	0.0	3.550602386324358E-5	0.0	0.6708951974019532	0.0
44-45	0.0	3.550602386324358E-5	0.0	0.8422117625421035	0.0
46-47	0.0	3.550602386324358E-5	0.0	1.0539519358505565	0.0
48-49	0.0	3.550602386324358E-5	0.0	1.2718524042992825	0.0
50-51	0.0	3.550602386324358E-5	0.0	1.5027569539879213	0.0
52-53	0.0	3.550602386324358E-5	0.0	1.7333685789796882	0.0
54-55	0.0	3.550602386324358E-5	0.0	1.977792047254257	0.0
56-57	0.0	3.550602386324358E-5	0.0	2.2613076478022567	0.0
58-59	0.0	3.550602386324358E-5	0.0	2.580737591487928	0.0
60-61	0.0	3.550602386324358E-5	0.0	2.906398842361598	0.0
62-63	0.0	3.550602386324358E-5	0.0	3.234066183583541	0.0
64-65	0.0	3.550602386324358E-5	0.0	3.587209096927362	0.0
66-67	0.0	3.550602386324358E-5	0.0	3.9723784437958285	0.0
68-69	0.0	3.550602386324358E-5	0.0	4.405915871671999	0.0
70-71	0.0	3.550602386324358E-5	0.0	4.883560657692282	0.0
72-73	0.0	3.550602386324358E-5	0.0	5.342928717928962	0.0
74-75	0.0	3.550602386324358E-5	0.0	5.795532880619694	0.0
76-77	0.0	3.550602386324358E-5	0.0	6.2914011333877875	0.0
78-79	0.0	3.550602386324358E-5	0.0	6.837812211125209	0.0
80-81	0.0	3.550602386324358E-5	0.0	7.425969496419839	0.0
82-83	0.0	3.550602386324358E-5	0.0	8.028586609932775	0.0
84-85	0.0	3.550602386324358E-5	0.0	8.613743636210959	0.0
86-87	0.0	3.550602386324358E-5	0.0	9.218695270792903	0.0
88	0.0	3.550602386324358E-5	0.0	9.702677882072777	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2100	0.0	27.321337	1
TCTAACG	1040	0.0	23.505695	2
CTAACGC	1040	0.0	22.601631	3
CATGGGG	11905	0.0	21.679146	4
TGATCGC	1755	0.0	19.546602	9
CTAGTAC	2755	0.0	18.770428	3
CGACGAG	1160	0.0	18.635656	24-25
TAACGCC	1375	0.0	18.462492	4
ATGGGGG	8140	0.0	18.018467	5
CGCCTAC	1305	0.0	18.005304	7
TAGGCAT	3155	0.0	17.880083	5
GTATAAG	3280	0.0	17.635698	1
GGGCTAT	3840	0.0	17.500313	6
CGTTAAC	565	0.0	17.479631	1
GTGATCG	1975	0.0	17.13178	8
CTATTGA	3700	0.0	17.018787	9
TAGTACT	3465	0.0	16.959227	4
GCATCGT	2600	0.0	16.809189	8
TACCTGG	6190	0.0	16.784359	2
CTGTGCG	2965	0.0	16.641413	9
>>END_MODULE