FastQCFastQC Report
Mon 16 Jan 2017
ERR1146591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632847
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA236740.4202848044692143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146400.259904094678943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144350.25626472723296057No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG102630.1821991614542344No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79250.1406926195581027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.13250848105762503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16900.025.880081
GAGTACT175800.021.73489612-13
CTAACGC11950.019.283333
ACTGCGC21100.019.1556158
CATGGGG116300.019.0051824
GTACTTT199300.018.98350514-15
AGTACTT189600.018.96308512-13
TGATCGC15400.018.9211359
GGGCTAT38650.017.9974776
GTATAAG25700.017.7503911
TCTAACG12650.017.473372
GCTATTG39200.017.3844958
CGACGAG10350.017.25533324-25
CTATTGA38000.017.067579
TAGGCAT32250.016.9057855
TAACGCC15050.016.8737324
GGCTATT40600.016.7850287
CTCGTCG14700.016.6250239
ACTTTTT229300.016.61249516-17
CAATGCG11900.016.58755718-19
Produced by FastQC (version 0.11.5)