FastQCFastQC Report
Mon 16 Jan 2017
ERR1146590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4823216
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA147130.3050454302689326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.024.251731
TACCCCG2751.4315447E-920.5180845
CATGGGG109250.019.8852624
TAGTACT22650.018.4770494
ATGGGGG71450.017.4394135
CTAGTAC20900.017.1001783
GTATATA23550.016.7777791
GGGCTAT31400.016.3161746
TTTAGGG31250.016.1015282
GTACATG341800.016.1012611
TACATGG338750.016.0199012
ACATGGG324600.015.8055483
GGCTATT30650.015.7953117
GTATAAG22200.015.6792331
ATACGGG13950.015.5066143
AGGGCTA34500.015.4009535
TAGGCAT30250.015.3885635
GCTATTG31800.015.3715858
CTATTGA31350.015.29079159
TTAGGGC45250.015.2767893
Produced by FastQC (version 0.11.5)