##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146590_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4823216
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228104235845958	31.0	31.0	34.0	28.0	34.0
2	31.299796028210224	31.0	31.0	34.0	28.0	34.0
3	31.455779297464595	33.0	31.0	34.0	28.0	34.0
4	34.93009228697201	37.0	35.0	37.0	32.0	37.0
5	34.99765177425187	37.0	35.0	37.0	32.0	37.0
6	34.98298293089093	37.0	35.0	37.0	32.0	37.0
7	34.94508539530471	37.0	35.0	37.0	32.0	37.0
8	34.954907679855104	37.0	35.0	37.0	32.0	37.0
9	36.544588714252065	39.0	37.0	39.0	32.0	39.0
10-11	36.542500273676325	39.0	37.0	39.0	32.0	39.0
12-13	36.49674231881798	39.0	37.0	39.0	32.0	39.0
14-15	37.82222390620698	40.0	38.0	41.0	32.0	41.0
16-17	37.8346122172426	40.0	38.0	41.0	32.0	41.0
18-19	37.836732794052764	40.0	38.0	41.0	32.0	41.0
20-21	37.93174823603172	40.0	38.0	41.0	32.0	41.0
22-23	37.93797561626931	40.0	38.0	41.0	32.0	41.0
24-25	37.95501704671737	40.0	38.0	41.0	32.0	41.0
26-27	38.018242392627656	40.0	38.0	41.0	32.0	41.0
28-29	37.98845935989597	40.0	38.0	41.0	32.0	41.0
30-31	37.95109476747464	40.0	38.0	41.0	32.0	41.0
32-33	37.90730987374399	40.0	38.0	41.0	32.0	41.0
34-35	37.862679693383	40.0	38.0	41.0	31.0	41.0
36-37	37.79103548337872	40.0	38.0	41.0	31.0	41.0
38-39	37.72973561623614	40.0	38.0	41.0	31.0	41.0
40-41	37.66092209015727	40.0	37.5	41.0	31.0	41.0
42-43	37.48846174419723	40.0	37.0	41.0	30.5	41.0
44-45	37.339616450932326	40.0	37.0	41.0	30.0	41.0
46-47	37.207835809136476	40.0	36.5	41.0	30.0	41.0
48-49	37.09169493964193	40.0	36.0	41.0	30.0	41.0
50-51	36.95828208813373	40.0	36.0	41.0	30.0	41.0
52-53	36.82934394810434	39.0	35.0	41.0	30.0	41.0
54-55	36.70346548858687	39.0	35.0	41.0	30.0	41.0
56-57	36.50611531807823	39.0	35.0	41.0	30.0	41.0
58-59	36.30831285598654	39.0	35.0	40.5	29.0	41.0
60-61	36.08206371018839	38.0	35.0	40.0	29.0	41.0
62-63	35.822985223966754	37.5	35.0	40.0	29.0	41.0
64-65	35.537287569124004	37.0	34.0	40.0	28.0	41.0
66-67	35.29049911925985	36.5	34.0	40.0	28.0	41.0
68-69	35.03429309406836	36.0	34.0	39.0	28.0	41.0
70-71	34.73601265213915	36.0	34.0	39.0	28.0	41.0
72-73	34.395111166491404	35.0	34.0	38.5	28.0	40.5
74-75	34.069013703719676	35.0	34.0	37.5	27.0	40.0
76-77	33.789636002202684	35.0	33.0	37.0	27.0	39.0
78-79	33.48661225207414	35.0	33.0	36.5	26.5	39.0
80-81	33.26028960759792	35.0	33.0	36.0	26.5	38.5
82-83	33.01589551452807	35.0	33.0	36.0	26.0	37.0
84-85	32.8204465651134	35.0	33.0	35.0	26.0	37.0
86-87	32.61794340124929	35.0	33.0	35.0	26.0	36.5
88-89	32.44722100357936	35.0	33.0	35.0	26.0	36.0
90-91	32.301555124215874	35.0	33.0	35.0	25.0	36.0
92-93	32.143826961098156	35.0	33.0	35.0	25.0	36.0
94-95	32.0357239858219	35.0	33.0	35.0	25.0	35.5
96-97	31.948631576939533	35.0	32.5	35.0	25.0	35.0
98-99	31.83399986647913	35.0	32.0	35.0	24.5	35.0
100	30.620965140271554	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3448915691425434
1101	2	0.3147947548489327
1101	3	0.31307830958008154
1101	4	0.3138364546892589
1101	5	0.24227593867490071
1101	6	0.2887689768954331
1101	7	0.3655753827814934
1101	8	0.32833534791907226
1101	9	0.4244872757232159
1101	10-11	0.44448338372416885
1101	12-13	0.4092431136347514
1101	14-15	0.5602344301611666
1101	16-17	0.536242300573214
1101	18-19	0.5032542314215078
1101	20-21	0.5164568308024755
1101	22-23	0.5570190652984124
1101	24-25	0.5348229436194032
1101	26-27	0.5246026063891946
1101	28-29	0.5397170789877634
1101	30-31	0.5534994309992882
1101	32-33	0.5619525285331761
1101	34-35	0.5095952810496414
1101	36-37	0.5418571574396864
1101	38-39	0.4970431855309556
1101	40-41	0.5307034697648589
1101	42-43	0.4859793851879104
1101	44-45	0.44273483514475487
1101	46-47	0.40031576271673686
1101	48-49	0.45289729945833557
1101	50-51	0.40166346579082557
1101	52-53	0.3985509408377368
1101	54-55	0.3935028605833466
1101	56-57	0.40634364744452256
1101	58-59	0.4567515090958878
1101	60-61	0.47700949000545734
1101	62-63	0.3839614537203886
1101	64-65	0.46669370795252263
1101	66-67	0.37113689441767406
1101	68-69	0.4166180277342022
1101	70-71	0.3689830142830104
1101	72-73	0.4094079580043726
1101	74-75	0.4272688668476974
1101	76-77	0.3612210303204506
1101	78-79	0.3695321344323119
1101	80-81	0.3820767544646273
1101	82-83	0.4062766758669838
1101	84-85	0.2938688281757891
1101	86-87	0.31022974127109393
1101	88-89	0.33375145375011783
1101	90-91	0.3543579066749416
1101	92-93	0.4108980298794549
1101	94-95	0.37636195791453986
1101	96-97	0.3949849395482836
1101	98-99	0.4028293095835167
1101	100	0.4422859477198493
1102	1	0.28897389703115905
1102	2	0.3330681567600209
1102	3	0.3051003404301902
1102	4	0.33847899068269527
1102	5	0.3305654721683169
1102	6	0.2808137352428446
1102	7	0.34642178442577887
1102	8	0.3590061055352507
1102	9	0.5189720285369788
1102	10-11	0.4855730931086484
1102	12-13	0.4742302923618169
1102	14-15	0.5884187501643297
1102	16-17	0.5314096811797384
1102	18-19	0.47213386461946527
1102	20-21	0.45473821204142695
1102	22-23	0.42454276859587026
1102	24-25	0.5424428311677048
1102	26-27	0.5281847193547407
1102	28-29	0.4989171451939569
1102	30-31	0.5137842411258688
1102	32-33	0.5698352645285993
1102	34-35	0.5079671770169938
1102	36-37	0.524492176066353
1102	38-39	0.5175756265629872
1102	40-41	0.473323332609354
1102	42-43	0.5265432741591454
1102	44-45	0.48887418681818673
1102	46-47	0.45817039813803717
1102	48-49	0.4772447485437965
1102	50-51	0.48943150203624697
1102	52-53	0.45726261288982784
1102	54-55	0.3789928357807355
1102	56-57	0.44051091935433107
1102	58-59	0.44841854631958
1102	60-61	0.44289996323148273
1102	62-63	0.44505556038152605
1102	64-65	0.47571176890174627
1102	66-67	0.44518493342991405
1102	68-69	0.38720426129382446
1102	70-71	0.4007628490639874
1102	72-73	0.41932709011041425
1102	74-75	0.3926116567962197
1102	76-77	0.3952596571843472
1102	78-79	0.33344722993721376
1102	80-81	0.2713531430652978
1102	82-83	0.3059890618589449
1102	84-85	0.3211878357746798
1102	86-87	0.32995418271814714
1102	88-89	0.3341652424239214
1102	90-91	0.29471656881587194
1102	92-93	0.29749158564581535
1102	94-95	0.2486510862656104
1102	96-97	0.20598344249791367
1102	98-99	0.2285412731404648
1102	100	0.26649097939012023
1103	1	0.250834403293414
1103	2	0.32330834085969684
1103	3	0.3058457610325185
1103	4	0.2777465645425963
1103	5	0.29855109216769193
1103	6	0.2908782889516104
1103	7	0.28284084599033577
1103	8	0.30553928902552485
1103	9	0.4257847056203232
1103	10-11	0.3987537089000668
1103	12-13	0.4247025007788494
1103	14-15	0.4789982868153402
1103	16-17	0.5016753324331731
1103	18-19	0.47190391789818165
1103	20-21	0.454522009234843
1103	22-23	0.4514864385863362
1103	24-25	0.5046325510278251
1103	26-27	0.46569530151684546
1103	28-29	0.48890151934718773
1103	30-31	0.5414312105926768
1103	32-33	0.5060386094819975
1103	34-35	0.46955709773511956
1103	36-37	0.4547226101689077
1103	38-39	0.4784287596122567
1103	40-41	0.4534131198609046
1103	42-43	0.463788500342595
1103	44-45	0.40295399141031396
1103	46-47	0.4356563479795099
1103	48-49	0.38405535040274685
1103	50-51	0.39473934497330276
1103	52-53	0.3798096057533158
1103	54-55	0.3317844335647706
1103	56-57	0.42231219752653715
1103	58-59	0.4361127858405567
1103	60-61	0.4193645587767705
1103	62-63	0.34121755737825765
1103	64-65	0.3343940393585001
1103	66-67	0.3180233174443785
1103	68-69	0.31446286733832807
1103	70-71	0.3709070581858924
1103	72-73	0.3500070533919839
1103	74-75	0.4084206925129621
1103	76-77	0.3887376775341167
1103	78-79	0.4530966403422454
1103	80-81	0.34442793664272386
1103	82-83	0.3791551415928609
1103	84-85	0.3062336146318927
1103	86-87	0.3205533332042876
1103	88-89	0.3152216099060752
1103	90-91	0.33857174044700855
1103	92-93	0.3197231562598333
1103	94-95	0.3194124552638655
1103	96-97	0.28529535263762185
1103	98-99	0.3157113109795162
1103	100	0.3250816316544949
1104	1	0.13013360624698578
1104	2	0.11656322993064805
1104	3	0.1168776486768408
1104	4	0.1208043000929564
1104	5	0.0917364027298575
1104	6	0.11059968533862019
1104	7	0.13306756261664532
1104	8	0.17877527260898773
1104	9	0.2042409243636243
1104	10-11	0.24588096681745952
1104	12-13	0.2079405457753083
1104	14-15	0.2596006411365579
1104	16-17	0.2403576219066892
1104	18-19	0.22649587220571732
1104	20-21	0.2686382780272183
1104	22-23	0.27194231174306793
1104	24-25	0.29564816732323607
1104	26-27	0.30201331193157444
1104	28-29	0.2250359781967859
1104	30-31	0.2247753373162098
1104	32-33	0.31911312968352235
1104	34-35	0.23865712455766186
1104	36-37	0.28480708020805423
1104	38-39	0.246003768759131
1104	40-41	0.2830470479914524
1104	42-43	0.3001413665303687
1104	44-45	0.1958682939025067
1104	46-47	0.1686927228389834
1104	48-49	0.2439935128910946
1104	50-51	0.19401995906816438
1104	52-53	0.16099287853878508
1104	54-55	0.13000866496559382
1104	56-57	0.1534046535066551
1104	58-59	0.16721221519257057
1104	60-61	0.14263429946689143
1104	62-63	0.13423220325524454
1104	64-65	0.1334592660619336
1104	66-67	0.10362584549014286
1104	68-69	0.09019107860546427
1104	70-71	0.10051377575366871
1104	72-73	0.10190285157067791
1104	74-75	0.06436679582046168
1104	76-77	0.060520818395367826
1104	78-79	0.09604222766456871
1104	80-81	0.0871578638471604
1104	82-83	0.10613381813024603
1104	84-85	0.026702931207964298
1104	86-87	0.0055595947157556225
1104	88-89	0.008326772145842654
1104	90-91	0.003500633228760819
1104	92-93	0.03470725619585835
1104	94-95	0.030581267116367883
1104	96-97	0.06160297461292075
1104	98-99	0.0828353236736703
1104	100	0.16502396619262072
1105	1	0.04488177353650613
1105	2	0.06847698077940123
1105	3	0.08201897817052739
1105	4	0.1047885414990688
1105	5	0.04855584408392133
1105	6	0.06377508474390226
1105	7	0.05640277728858223
1105	8	0.13753492013919555
1105	9	0.11627475486433525
1105	10-11	0.13483148498999498
1105	12-13	0.15626743900559603
1105	14-15	0.2125307507010561
1105	16-17	0.20555065011730989
1105	18-19	0.2210767027722369
1105	20-21	0.2248408323882174
1105	22-23	0.18580391460741197
1105	24-25	0.23149220708202733
1105	26-27	0.19065662512299753
1105	28-29	0.1985168752391644
1105	30-31	0.22609305394451695
1105	32-33	0.22185392711524088
1105	34-35	0.19882322595454127
1105	36-37	0.1724190449262082
1105	38-39	0.18910540261080655
1105	40-41	0.19395992103574855
1105	42-43	0.19228568111201838
1105	44-45	0.1226239373601743
1105	46-47	0.13853113006349105
1105	48-49	0.1320502906906924
1105	50-51	0.09446853553554035
1105	52-53	0.13583520702007235
1105	54-55	0.08295403006346191
1105	56-57	0.09413088918625334
1105	58-59	0.12141783618154278
1105	60-61	0.14244459930137054
1105	62-63	0.13646601732038022
1105	64-65	0.07050172098874441
1105	66-67	0.09862301922982653
1105	68-69	0.09148993577996833
1105	70-71	0.09585789327847039
1105	72-73	0.07585748873722054
1105	74-75	0.09005316990761969
1105	76-77	0.09274836121370811
1105	78-79	0.08701642688993161
1105	80-81	0.018530756603517773
1105	82-83	0.0774450381338525
1105	84-85	0.038855148900069025
1105	86-87	-0.00899805381865093
1105	88-89	0.010409561746101303
1105	90-91	0.04085547045010429
1105	92-93	0.0013813681297989433
1105	94-95	0.03529082568076802
1105	96-97	0.021042519272992166
1105	98-99	0.008019136904266588
1105	100	0.14342661723223316
1106	1	0.08252068518542188
1106	2	0.05046865672250789
1106	3	0.0987946542144158
1106	4	0.04986870324183457
1106	5	0.013401287619281277
1106	6	0.05592598288641426
1106	7	0.09713315349259233
1106	8	0.1546378529027308
1106	9	0.14589142703573543
1106	10-11	0.1494360323466637
1106	12-13	0.16288535581318087
1106	14-15	0.24884136296432047
1106	16-17	0.2057038955028574
1106	18-19	0.26230056487902687
1106	20-21	0.292701310815751
1106	22-23	0.2631031567010851
1106	24-25	0.24058019486466264
1106	26-27	0.2253992965159881
1106	28-29	0.13803382513988538
1106	30-31	0.1900291846326283
1106	32-33	0.2212348905312993
1106	34-35	0.2041315977507736
1106	36-37	0.20131318603326775
1106	38-39	0.12514495016101534
1106	40-41	0.14738921323489507
1106	42-43	0.15205823911418292
1106	44-45	0.13063148624981125
1106	46-47	0.14256964988211962
1106	48-49	0.14658114128080513
1106	50-51	0.1795855273745417
1106	52-53	0.1502227177040254
1106	54-55	0.09489157535563209
1106	56-57	0.19506441204104163
1106	58-59	0.14123394578268034
1106	60-61	0.20400838291816115
1106	62-63	0.20183051813450703
1106	64-65	0.22902222639780234
1106	66-67	0.2541696559030413
1106	68-69	0.20320241842124886
1106	70-71	0.14644449576711338
1106	72-73	0.20326336966603975
1106	74-75	0.2445687833305925
1106	76-77	0.2133413143845715
1106	78-79	0.23494429711941223
1106	80-81	0.20727046163561624
1106	82-83	0.2186658016034997
1106	84-85	0.21016288654242032
1106	86-87	0.16478317853879076
1106	88-89	0.18202977408503784
1106	90-91	0.14598139855542058
1106	92-93	0.12283723586655526
1106	94-95	0.13686522797144818
1106	96-97	0.14478539456645834
1106	98-99	0.1826602359878109
1106	100	0.14216239359881655
1107	1	0.06922192469714972
1107	2	0.11309311618121498
1107	3	0.12303314482388927
1107	4	0.06769191323871837
1107	5	0.062484899049451315
1107	6	0.07975888375535334
1107	7	0.07352875581854335
1107	8	0.10822520212531828
1107	9	0.1629129842353194
1107	10-11	0.12377190095401858
1107	12-13	0.18015098037826505
1107	14-15	0.1889226777188142
1107	16-17	0.21492988106573563
1107	18-19	0.2260372627077487
1107	20-21	0.2506515675696832
1107	22-23	0.27429992106291223
1107	24-25	0.28470073728816203
1107	26-27	0.1878403359485361
1107	28-29	0.1552966073816151
1107	30-31	0.22478761380715184
1107	32-33	0.24769085400404123
1107	34-35	0.20006879055932814
1107	36-37	0.21886791408721962
1107	38-39	0.18943677530191394
1107	40-41	0.18691028841564616
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2216	60-61	-0.4499974266359885
2216	62-63	-0.5086445826852071
2216	64-65	-0.5239647362605027
2216	66-67	-0.4841483027030904
2216	68-69	-0.5402757898578514
2216	70-71	-0.4922034922853342
2216	72-73	-0.4272421939787989
2216	74-75	-0.4571734393561684
2216	76-77	-0.4696306735093856
2216	78-79	-0.39655516969585136
2216	80-81	-0.470359769694376
2216	82-83	-0.460391471345595
2216	84-85	-0.4037811056833789
2216	86-87	-0.45662275589680945
2216	88-89	-0.4147760333911208
2216	90-91	-0.4511057915118144
2216	92-93	-0.39770823696358804
2216	94-95	-0.4470180316717993
2216	96-97	-0.4420696653306564
2216	98-99	-0.37760725503395776
2216	100	-0.3846455050563087
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1439.0
1	355.0
2	264.0
3	256.0
4	204.0
5	289.0
6	475.0
7	1097.0
8	3123.0
9	4853.0
10	5560.0
11	6403.0
12	7395.0
13	9086.0
14	11546.0
15	13575.0
16	14379.0
17	15703.0
18	17223.0
19	19261.0
20	21433.0
21	23659.0
22	26273.0
23	30671.0
24	37370.0
25	44653.0
26	46654.0
27	44687.0
28	49081.0
29	59767.0
30	70221.0
31	82987.0
32	105427.0
33	142910.0
34	213162.0
35	333326.0
36	611490.0
37	1162659.0
38	1236555.0
39	347204.0
40	541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80245272465582	19.706993004816	13.17086989953957	24.319684370988615
2	15.002647294116866	20.97024159255903	39.3486245688811	24.678486544443007
3	18.134665676456507	26.45529456365908	30.179808635454297	25.230231124430112
4	12.631663916492084	17.809697602533923	38.41975909134675	31.13887938962725
5	13.465415279558604	38.20003780805419	34.862989380335954	13.471557532051248
6	30.131357148297095	37.45497626299403	18.60206393770128	13.811602651007595
7	26.48489056538623	32.95098901399905	22.95975009140821	17.60437032920651
8	25.95142712772194	34.76715339145809	20.96699588810414	18.314423592715833
9	26.474576151620838	16.056826690917102	20.514483556780526	36.95411360068154
10-11	24.332617500995728	26.58402934099885	28.264708497433723	20.8186446605717
12-13	25.188500856454276	24.445835906015763	27.715372939364364	22.650290298165597
14-15	23.2839808471782	25.60032568055274	25.213393280742924	25.902300191526145
16-17	22.678168199806716	28.26692147241619	26.38457512230624	22.670335205470852
18-19	22.663126375048062	28.125876020904776	27.361793858417798	21.84920374562936
20-21	23.61330445945771	27.381438593306274	26.55522388059702	22.450033066639
22-23	23.50009393945776	27.164832469792316	25.945891494791002	23.389182095958923
24-25	23.235232724567037	27.51955918840936	26.177338024854997	23.06787006216861
26-27	23.365580094695577	27.318473604653175	26.157586046028637	23.158360254622607
28-29	23.083290813918094	27.392560060273475	26.49396694628153	23.030182179526896
30-31	23.49000022099418	27.33416799271819	26.291127353549076	22.884704432738552
32-33	23.076132943616177	27.52172860608122	26.0939784714852	23.308159978817404
34-35	23.183793684528133	27.594613430854665	26.48562854910284	22.735964335514357
36-37	23.506371490336804	27.27821698915371	26.256558124188423	22.958853396321068
38-39	23.662603383582493	27.04122622518708	26.308432456336128	22.987737934894295
40-41	23.237301898072378	27.04829398841721	26.58750786203164	23.12689625147877
42-43	23.446221753684032	27.012308120121943	26.343994421813033	23.19747570438099
44-45	23.264259531948024	27.359569051528215	26.4078268469991	22.96834456952466
46-47	23.08963609033041	27.222797247955434	26.552444975294183	23.135121686419975
48-49	23.422292306477473	27.039139326422607	26.509249644791183	23.02931872230873
50-51	23.051072458649287	27.132224850925347	26.348483718129195	23.46821897229617
52-53	22.769148650925878	27.38924916219853	26.109542178956218	23.732060007919372
54-55	22.848855915764904	27.36324979251149	26.154177219208822	23.633717072514784
56-57	23.21653609855018	27.002919818458455	26.137744996157096	23.642799086834273
58-59	23.015379538707233	26.860129316383087	26.72149451635029	23.402996628559393
60-61	23.097485741869995	27.108969448657973	26.370375381941063	23.423169427530972
62-63	22.96343314921702	27.005773571282628	26.344540426776696	23.68625285272366
64-65	22.493622522439598	27.111058441800353	26.528563306303532	23.86675572945652
66-67	23.21379927971363	26.839949794763523	26.27729416812943	23.668956757393417
68-69	23.12681051289789	26.779807595323412	26.31729794997744	23.77608394180126
70-71	22.81691757349547	26.9586651269671	26.359713971196218	23.864703328341207
72-73	23.264827893793687	26.902972401596266	26.40525288964032	23.42694681496973
74-75	23.238583704522206	27.11162564278553	26.294358077863066	23.355432574829198
76-77	22.968329114381163	26.99265327435586	26.494867420024793	23.54415019123818
78-79	22.995035384521344	27.033124660841906	26.522341807429704	23.44949814720704
80-81	22.823591113706808	27.03307304062623	26.6614842651227	23.481851580544262
82-83	22.98385402024342	26.767749883484065	26.677691964349187	23.570704131923332
84-85	23.13170166169663	26.998460838552425	26.165827627200816	23.704009872550127
86-87	22.93489720611492	26.95571955719557	26.372027527924324	23.737355708765186
88-89	23.201150835108812	26.43447615750743	26.46490110646366	23.899471900920094
90-91	23.269458362453495	26.53908275521063	26.51033875889235	23.68112012344353
92-93	23.156733466765438	26.68804250458191	26.469642467476312	23.685581561176342
94-95	22.647542540044554	26.717441132920843	26.72250474454183	23.912511582492773
96-97	23.106754309964668	26.54288693703275	26.465751150447986	23.884607602554592
98-99	23.143107834575787	26.707709837752347	26.23644498684387	23.91273734082799
100	23.202808911931143	26.70210161250966	26.048218574019515	24.04687090153968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4190.0
1	2834.5
2	1466.0
3	1417.0
4	1261.5
5	1039.0
6	897.5
7	892.0
8	938.5
9	978.0
10	898.0
11	774.5
12	778.5
13	831.0
14	929.5
15	1080.0
16	1227.0
17	1469.0
18	1857.5
19	2270.5
20	2892.0
21	3983.0
22	5707.0
23	8041.5
24	10727.0
25	13936.5
26	18506.5
27	24079.0
28	29918.0
29	37846.0
30	47835.0
31	57839.0
32	69329.5
33	79433.5
34	86588.5
35	94338.0
36	103261.0
37	115605.5
38	124354.5
39	126802.0
40	130060.0
41	132566.0
42	135340.5
43	143236.0
44	154473.0
45	168839.5
46	181557.5
47	190534.0
48	204703.0
49	213449.5
50	227873.0
51	227205.0
52	199603.5
53	186843.5
54	179117.0
55	162760.0
56	148476.0
57	133043.5
58	117068.0
59	103888.0
60	87137.5
61	69978.5
62	55675.5
63	43673.5
64	32240.5
65	22826.0
66	17168.0
67	13494.5
68	10533.5
69	8339.0
70	6846.0
71	5527.0
72	4650.5
73	4281.5
74	3561.5
75	2811.5
76	2162.5
77	1470.5
78	904.5
79	614.5
80	436.5
81	315.0
82	207.5
83	132.5
84	110.0
85	88.5
86	58.0
87	52.0
88	50.5
89	43.0
90	32.0
91	24.0
92	19.5
93	22.0
94	28.5
95	22.0
96	16.0
97	15.0
98	12.5
99	15.0
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1016126999081111
2	0.0663457742717722
3	0.08369934085473262
4	0.08558604881058614
5	0.0857726463007255
6	0.09058271493542898
7	0.08653976931574285
8	0.08855087559835595
9	0.07275228809989019
10-11	0.08053755004959347
12-13	0.06933133411400194
14-15	0.07815324878670166
16-17	0.07999849063363532
18-19	0.0795941960716667
20-21	0.0705649508543677
22-23	0.07510548978109212
24-25	0.07758308978905362
26-27	0.071643069686284
28-29	0.079801526616266
30-31	0.08468416094157924
32-33	0.08636353835283346
34-35	0.08924543292276357
36-37	0.08891370405140471
38-39	0.08976375928426178
40-41	0.08643610404344322
42-43	0.0921791601288435
44-45	0.08840574421713644
46-47	0.08517138772138756
48-49	0.09478115846356455
50-51	0.09133947142321637
52-53	0.09645016934758882
54-55	0.0886234412889657
56-57	0.09525801871614292
58-59	0.09953939446211824
60-61	0.09821247897668277
62-63	0.09940462960812868
64-65	0.1014364689452017
66-67	0.10353050744565451
68-69	0.10014065304145615
70-71	0.10283595012124691
72-73	0.10033761705882548
74-75	0.10421469824283217
76-77	0.10115657270999268
78-79	0.10821617775359843
80-81	0.10338537606443501
82-83	0.10792591499115942
84-85	0.1038311367353235
86-87	0.10026505136821573
88-89	0.09956012751657815
90-91	0.09990222291516697
92-93	0.09403476850300713
94-95	0.09373413921333815
96-97	0.09207549485654386
98-99	0.1061221392531456
100	0.24792586523182872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4823216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.90395819891375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.78649109464912	39.57245039203045
2	14.743068905427773	16.483918156254784
3	5.901552897497046	9.897604994710596
4	2.974601260082709	6.651679380083997
5	1.744310004334322	4.87569167841265
6	1.056263408027302	3.542958324564028
7	0.6961474024619851	2.724217670126204
8	0.4783533648551349	2.139347721053696
9	0.33779466551199533	1.6995652974538773
>10	1.2596522751948418	10.703834127951634
>50	0.017830580468052606	0.6707195712225589
>100	0.0036779754364734568	0.2991363591288617
>500	6.404151332426038E-5	0.019440345275439242
>1k	1.2808302657314223E-4	0.19268496908073499
>5k	0.0	0.0
>10k+	6.404151328657112E-5	0.5267510126504745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14713	0.3050454302689326	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.146610891985762E-5
6	0.0	0.0	0.0	0.0	4.146610891985762E-5
7	0.0	0.0	0.0	0.0	4.146610891985762E-5
8	0.0	0.0	0.0	0.0	4.146610891985762E-5
9	0.0	0.0	0.0	0.0	4.146610891985762E-5
10-11	0.0	0.0	0.0	2.073305445992881E-5	4.146610891985762E-5
12-13	0.0	0.0	0.0	2.073305445992881E-5	6.219916337978643E-5
14-15	0.0	0.0	0.0	3.1099581689893215E-5	6.219916337978643E-5
16-17	0.0	0.0	0.0	5.183263614982203E-5	8.293221783971525E-5
18-19	0.0	0.0	0.0	1.4513138121950166E-4	8.293221783971525E-5
20-21	0.0	0.0	0.0	2.0733054459928812E-4	1.0366527229964406E-4
22-23	0.0	0.0	0.0	5.079598342682559E-4	1.0366527229964406E-4
24-25	0.0	0.0	0.0	0.001534246030034732	1.0366527229964406E-4
26-27	0.0	0.0	0.0	0.002923360678849962	1.451313812195017E-4
28-29	0.0	0.0	0.0	0.009184743125748462	1.451313812195017E-4
30-31	0.0	0.0	0.0	0.02349055070309934	1.451313812195017E-4
32-33	0.0	0.0	0.0	0.052174731548410846	1.554979084494661E-4
34-35	0.0	0.0	0.0	0.0985234747935817	1.865974901393593E-4
36-37	0.0	0.0	0.0	0.1701458114254058	1.865974901393593E-4
38-39	0.0	0.0	0.0	0.2739354820518094	1.865974901393593E-4
40-41	0.0	0.0	0.0	0.3949646874616438	1.865974901393593E-4
42-43	0.0	1.0366527229964406E-5	0.0	0.512002779887942	1.969640173693237E-4
44-45	0.0	2.073305445992881E-5	0.0	0.6583263117388896	2.1769707182925252E-4
46-47	0.0	2.073305445992881E-5	0.0	0.8367653449482668	2.2806359905921692E-4
48-49	0.0	2.073305445992881E-5	0.0	1.02808997150449	2.591631807491101E-4
50-51	0.0	2.073305445992881E-5	0.0	1.2262461395052595	2.902627624390034E-4
52-53	0.0	2.073305445992881E-5	0.0	1.4285281853435552	2.902627624390034E-4
54-55	0.0	2.073305445992881E-5	0.0	1.6529531333450544	2.902627624390034E-4
56-57	0.0	2.073305445992881E-5	0.0	1.9183258639049132	3.2136234412889655E-4
58-59	0.0	2.073305445992881E-5	0.0	2.2155756656969126	3.31728871358861E-4
60-61	0.0	2.073305445992881E-5	0.0	2.5257836265263673	3.524619258187898E-4
62-63	0.0	2.073305445992881E-5	0.0	2.84250176645624	3.524619258187898E-4
64-65	0.0	2.073305445992881E-5	0.0	3.1784809139793864	3.731949802787186E-4
66-67	0.0	2.073305445992881E-5	0.0	3.5588287980467808	3.83561507508683E-4
68-69	0.0	2.073305445992881E-5	0.0	3.98413631071053	3.939280347386474E-4
70-71	0.0	2.073305445992881E-5	0.0	4.457461577503475	4.1466108919857624E-4
72-73	1.0366527229964406E-5	2.073305445992881E-5	0.0	4.924287031723232	4.1466108919857624E-4
74-75	2.073305445992881E-5	2.073305445992881E-5	0.0	5.394657838255637	4.250276164285406E-4
76-77	2.073305445992881E-5	2.073305445992881E-5	0.0	5.920282234923752	4.3539414365850504E-4
78-79	2.073305445992881E-5	4.146610891985762E-5	0.0	6.484853674394843	4.4576067088846947E-4
80-81	2.073305445992881E-5	4.146610891985762E-5	0.0	7.090922322367483	4.5612719811843384E-4
82-83	2.073305445992881E-5	4.146610891985762E-5	0.0	7.731501139488673	4.5612719811843384E-4
84-85	2.073305445992881E-5	4.146610891985762E-5	0.0	8.366772294668122	4.5612719811843384E-4
86-87	2.073305445992881E-5	6.219916337978643E-5	0.0	9.024186352010775	4.5612719811843384E-4
88	2.073305445992881E-5	6.219916337978643E-5	0.0	9.543300569578472	4.5612719811843384E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1765	0.0	24.25173	1
TACCCCG	275	1.4315447E-9	20.518084	5
CATGGGG	10925	0.0	19.885262	4
TAGTACT	2265	0.0	18.477049	4
ATGGGGG	7145	0.0	17.439413	5
CTAGTAC	2090	0.0	17.100178	3
GTATATA	2355	0.0	16.777779	1
GGGCTAT	3140	0.0	16.316174	6
TTTAGGG	3125	0.0	16.101528	2
GTACATG	34180	0.0	16.101261	1
TACATGG	33875	0.0	16.019901	2
ACATGGG	32460	0.0	15.805548	3
GGCTATT	3065	0.0	15.795311	7
GTATAAG	2220	0.0	15.679233	1
ATACGGG	1395	0.0	15.506614	3
AGGGCTA	3450	0.0	15.400953	5
TAGGCAT	3025	0.0	15.388563	5
GCTATTG	3180	0.0	15.371585	8
CTATTGA	3135	0.0	15.2907915	9
TTAGGGC	4525	0.0	15.276789	3
>>END_MODULE