FastQCFastQC Report
Mon 16 Jan 2017
ERR1146590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4823216
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170700.3539132396309848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156590.32465889978802526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83220.17254047921552756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT175150.023.38506512-13
GTACTAG17350.021.4126381
GTACTTT194550.020.84783214-15
AGTACTT186300.020.01775712-13
GTATCAA385800.019.3688681
CATGGGG110400.018.7403034
ATACGGG16300.018.462283
ACTTTTT223100.018.3695416-17
GGTATCA307150.018.35741
TGATCGC13650.017.9038969
TACTTTT210200.017.83114814-15
GTATAAG25700.017.5663151
CAACGCA424650.017.3433385
TACGGGC16950.017.1994674
CGGGCGT16750.017.1157676
ATCAACG429350.017.1065543
AACGCAG435600.017.079476
TATCAAC437200.017.0370752
CTATTGA29900.016.8185849
TCAACGC441250.016.7411174
Produced by FastQC (version 0.11.5)