FastQCFastQC Report
Mon 16 Jan 2017
ERR1146589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4776701
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA123160.2578348529665139No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA100490.21037531970286605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18350.020.2444441
CATGGGG81950.017.8973354
CTCGTCG8750.017.1847299
ACCGTAT3101.2644523E-716.6745648
GTATAAG26600.016.0869831
GTATAAT23150.016.0468921
GTACATG249750.015.9851621
GCATCGT16700.015.757788
TACATGG243850.015.6343872
GTAGGAC21350.015.6330333
TAGTACT24900.015.4809394
ACATGGG233000.015.333473
CTGTGCG20750.015.1725119
ATGGGGG57450.015.055565
CGTTAAC2851.742015E-514.8495221
CTAGTAC23450.014.8344573
GTGTAGC28250.014.8144791
TACTAGG26700.014.7894092
CGATAAT8900.014.78778166-67
GCTATTG26900.014.67406758
Produced by FastQC (version 0.11.5)