##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146589_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4776701
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31684922292603	31.0	31.0	34.0	28.0	34.0
2	31.457931321219395	33.0	31.0	34.0	28.0	34.0
3	31.496604665018808	33.0	31.0	34.0	28.0	34.0
4	34.956332205009275	37.0	35.0	37.0	32.0	37.0
5	34.98215567606179	37.0	35.0	37.0	32.0	37.0
6	35.0843165607393	37.0	35.0	37.0	32.0	37.0
7	34.963289935878336	37.0	35.0	37.0	32.0	37.0
8	34.99154542015504	37.0	35.0	37.0	32.0	37.0
9	36.585157203685135	39.0	37.0	39.0	32.0	39.0
10-11	36.58230356055361	39.0	37.0	39.0	32.0	39.0
12-13	36.56567503387798	39.0	37.0	39.0	32.0	39.0
14-15	37.90869001430066	40.0	38.0	41.0	32.0	41.0
16-17	37.92169763190118	40.0	38.0	41.0	32.0	41.0
18-19	37.94389349469435	40.0	38.0	41.0	32.0	41.0
20-21	38.024571037626174	40.0	38.0	41.0	32.0	41.0
22-23	38.0313480998706	40.0	38.0	41.0	32.0	41.0
24-25	38.04587224530068	40.0	38.0	41.0	32.0	41.0
26-27	38.088099820357186	40.0	38.0	41.0	32.0	41.0
28-29	38.05462200795068	40.0	38.0	41.0	32.0	41.0
30-31	38.03073010012559	40.0	38.0	41.0	32.0	41.0
32-33	37.997812402325366	40.0	38.0	41.0	32.0	41.0
34-35	37.96209559694023	40.0	38.0	41.0	32.0	41.0
36-37	37.897018465254575	40.0	38.0	41.0	32.0	41.0
38-39	37.840250520181186	40.0	38.0	41.0	31.0	41.0
40-41	37.78267637015588	40.0	38.0	41.0	31.0	41.0
42-43	37.6569255643173	40.0	38.0	41.0	31.0	41.0
44-45	37.57837145343617	40.0	37.0	41.0	31.0	41.0
46-47	37.47702535704035	40.0	37.0	41.0	30.0	41.0
48-49	37.38660563012003	40.0	37.0	41.0	30.0	41.0
50-51	37.275117596851885	40.0	37.0	41.0	30.0	41.0
52-53	37.15998447464055	40.0	36.0	41.0	30.0	41.0
54-55	37.061359503138256	40.0	36.0	41.0	30.0	41.0
56-57	36.88985557186854	39.5	35.5	41.0	30.0	41.0
58-59	36.7331466842911	39.0	35.0	41.0	30.0	41.0
60-61	36.52513711869342	39.0	35.0	41.0	30.0	41.0
62-63	36.28548510781813	39.0	35.0	41.0	29.0	41.0
64-65	35.99588617751038	38.0	35.0	40.0	29.0	41.0
66-67	35.74915543175091	37.5	35.0	40.0	29.0	41.0
68-69	35.49627263670052	37.0	34.5	40.0	29.0	41.0
70-71	35.17342000263362	36.5	34.0	39.0	28.0	41.0
72-73	34.81183467418204	36.0	34.0	39.0	28.0	41.0
74-75	34.46791907217973	35.5	34.0	39.0	28.0	40.0
76-77	34.150691345344825	35.0	34.0	37.5	27.5	39.5
78-79	33.815547592365526	35.0	34.0	37.0	27.0	39.0
80-81	33.557412113506786	35.0	33.5	36.5	27.0	39.0
82-83	33.28024058864057	35.0	33.0	36.0	26.5	38.0
84-85	33.06593682543664	35.0	33.0	36.0	26.0	37.0
86-87	32.842083898489776	35.0	33.0	35.5	26.0	37.0
88-89	32.64201056335743	35.0	33.0	35.0	26.0	36.5
90-91	32.48234178777361	35.0	33.0	35.0	26.0	36.0
92-93	32.33009413819286	35.0	33.0	35.0	25.0	36.0
94-95	32.2187923213113	35.0	33.0	35.0	25.0	36.0
96-97	32.123736235531595	35.0	33.0	35.0	25.0	35.5
98-99	32.01940293101871	35.0	33.0	35.0	25.0	35.0
100	30.878883564200482	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28789452866423915
1101	2	0.32621866196846483
1101	3	0.22964478277647515
1101	4	0.21130533199781354
1101	5	0.20485342591541666
1101	6	0.2751610104176194
1101	7	0.29291896082103364
1101	8	0.4078210268146236
1101	9	0.5035685217015953
1101	10-11	0.4927181582672233
1101	12-13	0.48568959262605205
1101	14-15	0.5623398657577425
1101	16-17	0.6029505791460679
1101	18-19	0.5275793784538649
1101	20-21	0.5439057618312191
1101	22-23	0.5704793184119623
1101	24-25	0.614613481878834
1101	26-27	0.5998009064692482
1101	28-29	0.6429183869655191
1101	30-31	0.6656428989814742
1101	32-33	0.6861419869325758
1101	34-35	0.5924930519985239
1101	36-37	0.6266817584968294
1101	38-39	0.6218143628686406
1101	40-41	0.621766446329211
1101	42-43	0.6316096025994327
1101	44-45	0.5412360023266771
1101	46-47	0.5631814373571373
1101	48-49	0.5679949145330596
1101	50-51	0.6074389417967652
1101	52-53	0.5425745644375937
1101	54-55	0.507306348797286
1101	56-57	0.5032887715181715
1101	58-59	0.5250443496184829
1101	60-61	0.5624039201910804
1101	62-63	0.5425929233154179
1101	64-65	0.5516720575500713
1101	66-67	0.4922817609053354
1101	68-69	0.4510419572216193
1101	70-71	0.5136814122269371
1101	72-73	0.4881814811667695
1101	74-75	0.5184554965851547
1101	76-77	0.5145173952715254
1101	78-79	0.49343281782822146
1101	80-81	0.467124227433942
1101	82-83	0.4531030444105397
1101	84-85	0.4137274592062212
1101	86-87	0.4314803092698014
1101	88-89	0.4045166565245424
1101	90-91	0.40733838048934956
1101	92-93	0.4568521823963394
1101	94-95	0.40364064051955495
1101	96-97	0.3866235727024119
1101	98-99	0.44937137776698677
1101	100	0.4412479033422372
1102	1	0.30819266889861296
1102	2	0.4096361764648222
1102	3	0.37091316470613833
1102	4	0.3425541354495252
1102	5	0.32475721824539505
1102	6	0.3847234625335503
1102	7	0.3555398523682527
1102	8	0.39562125700736317
1102	9	0.5038011891413063
1102	10-11	0.44825677693771837
1102	12-13	0.45735454463026315
1102	14-15	0.5707911973720812
1102	16-17	0.5497497710271517
1102	18-19	0.5145816567476018
1102	20-21	0.5519693143793631
1102	22-23	0.5325604088472033
1102	24-25	0.5621924576086954
1102	26-27	0.5277330969293246
1102	28-29	0.5846034624891914
1102	30-31	0.6108269183037791
1102	32-33	0.5778637224439152
1102	34-35	0.551332967336414
1102	36-37	0.5673239676953017
1102	38-39	0.6014999903164266
1102	40-41	0.6087513789427064
1102	42-43	0.5918146014650674
1102	44-45	0.4917333160109223
1102	46-47	0.5752935931339493
1102	48-49	0.5584449219546315
1102	50-51	0.5417281785731731
1102	52-53	0.5135341887040994
1102	54-55	0.4087106555766127
1102	56-57	0.44306739104290926
1102	58-59	0.4151225091777846
1102	60-61	0.4636409189648134
1102	62-63	0.47265493810764525
1102	64-65	0.5133063227559376
1102	66-67	0.5396665662311406
1102	68-69	0.494018480001543
1102	70-71	0.4899324684662574
1102	72-73	0.50833401131068
1102	74-75	0.5406005785369743
1102	76-77	0.506361281821313
1102	78-79	0.42915881083742136
1102	80-81	0.3788925718382288
1102	82-83	0.43920892679503964
1102	84-85	0.4279692495375116
1102	86-87	0.4020860108088158
1102	88-89	0.38626478783429974
1102	90-91	0.4148751860997635
1102	92-93	0.4157621636549038
1102	94-95	0.40580459953100956
1102	96-97	0.4598062755242651
1102	98-99	0.4928017095950352
1102	100	0.5512569149987883
1103	1	0.18386803206526992
1103	2	0.28616624531903057
1103	3	0.19498759772836394
1103	4	0.23347343918580066
1103	5	0.23373627370324357
1103	6	0.2744192526701781
1103	7	0.25514079324651817
1103	8	0.3585127551236482
1103	9	0.44353762170379696
1103	10-11	0.3948285788522341
1103	12-13	0.3940878510108021
1103	14-15	0.4823863379534643
1103	16-17	0.5008172534589974
1103	18-19	0.4753175465325654
1103	20-21	0.46359121767353173
1103	22-23	0.42178854633441887
1103	24-25	0.4536239785832592
1103	26-27	0.3846864689999876
1103	28-29	0.4375054701814207
1103	30-31	0.4437888481172294
1103	32-33	0.4547639888586943
1103	34-35	0.4086770822014998
1103	36-37	0.4155169958097531
1103	38-39	0.4149806658408366
1103	40-41	0.298545002727586
1103	42-43	0.4060503712371215
1103	44-45	0.3605403655730157
1103	46-47	0.3379997480834973
1103	48-49	0.28559405763880363
1103	50-51	0.37507517278989155
1103	52-53	0.33642174213548515
1103	54-55	0.281453021890151
1103	56-57	0.33348958397815665
1103	58-59	0.3367408128032281
1103	60-61	0.30103919370186105
1103	62-63	0.34921617821461126
1103	64-65	0.27963252395010585
1103	66-67	0.302585804606025
1103	68-69	0.22955152064297124
1103	70-71	0.31487188578282854
1103	72-73	0.3142373649972541
1103	74-75	0.3072882081306787
1103	76-77	0.2744502281404877
1103	78-79	0.19404004117658502
1103	80-81	0.19223161103623454
1103	82-83	0.2551211277504777
1103	84-85	0.2985543902564274
1103	86-87	0.22708580595546835
1103	88-89	0.14474352431991377
1103	90-91	0.19752230542796667
1103	92-93	0.2103640334412944
1103	94-95	0.31557564679933137
1103	96-97	0.3321515018685304
1103	98-99	0.23195704866997602
1103	100	0.34639889083069164
1104	1	0.13078750447655452
1104	2	0.14935326943724903
1104	3	0.163103515581561
1104	4	0.18268066074290346
1104	5	0.11845670537903885
1104	6	0.18118858893731726
1104	7	0.23180485928460115
1104	8	0.30077807920171296
1104	9	0.3580091842698039
1104	10-11	0.3393099225168541
1104	12-13	0.3297814895997746
1104	14-15	0.4607142277000449
1104	16-17	0.45783269515145264
1104	18-19	0.3993201686514638
1104	20-21	0.3632459599960285
1104	22-23	0.41867134244371584
1104	24-25	0.42647777809711584
1104	26-27	0.4076747783441945
1104	28-29	0.41072646101865473
1104	30-31	0.41122110347085794
1104	32-33	0.4519680914648774
1104	34-35	0.4691020787130853
1104	36-37	0.46022811301229183
1104	38-39	0.4075872801537983
1104	40-41	0.36988364491993764
1104	42-43	0.4838902429985481
1104	44-45	0.3797415480512569
1104	46-47	0.36659575579927406
1104	48-49	0.3556548113582352
1104	50-51	0.4168848383367276
1104	52-53	0.4113269912649571
1104	54-55	0.38162540276373846
1104	56-57	0.3603653108260474
1104	58-59	0.3591747734809161
1104	60-61	0.38633802666508643
1104	62-63	0.42054020173343076
1104	64-65	0.42577340828855625
1104	66-67	0.3659652271492604
1104	68-69	0.3673379288608061
1104	70-71	0.36608872974083084
1104	72-73	0.356731572192885
1104	74-75	0.3740974848230607
1104	76-77	0.24662777029625005
1104	78-79	0.3098278028333752
1104	80-81	0.30844954716807393
1104	82-83	0.2521768682905403
1104	84-85	0.24825773375107474
1104	86-87	0.17810840302087172
1104	88-89	0.18299762865057545
1104	90-91	0.2554829452412122
1104	92-93	0.2274705076539334
1104	94-95	0.23387548717739293
1104	96-97	0.2286499970581204
1104	98-99	0.2668798762753539
1104	100	0.3467809149793446
1105	1	0.17605964120023643
1105	2	0.14243436705731938
1105	3	0.18650539516354314
1105	4	0.16155786979037856
1105	5	0.16218598305503207
1105	6	0.20966217418994404
1105	7	0.2620877811645741
1105	8	0.2833078479329103
1105	9	0.29169185320468927
1105	10-11	0.3595131770710438
1105	12-13	0.33517895754398097
1105	14-15	0.4506702790987944
1105	16-17	0.3848657429189615
1105	18-19	0.37709552789728207
1105	20-21	0.3764320415261082
1105	22-23	0.42345126057322346
1105	24-25	0.4233133595981684
1105	26-27	0.37644929495483126
1105	28-29	0.33217497009304964
1105	30-31	0.3662480172428104
1105	32-33	0.3735553141397716
1105	34-35	0.39533640016879446
1105	36-37	0.3826988774128637
1105	38-39	0.36988451071639616
1105	40-41	0.41640670965714577
1105	42-43	0.43724066156769936
1105	44-45	0.3511729152284886
1105	46-47	0.3865032160456323
1105	48-49	0.34163394261615565
1105	50-51	0.3559979013453969
1105	52-53	0.44547872785062026
1105	54-55	0.366512960527956
1105	56-57	0.380681251554158
1105	58-59	0.3201335894383064
1105	60-61	0.3511257221163788
1105	62-63	0.34702171751423805
1105	64-65	0.4287098605273485
1105	66-67	0.418965942186027
1105	68-69	0.39514119198042863
1105	70-71	0.4053542375284138
1105	72-73	0.3764780663824112
1105	74-75	0.34023498601791147
1105	76-77	0.3305405910003998
1105	78-79	0.3285047110753254
1105	80-81	0.3109230467664048
1105	82-83	0.28171155132660175
1105	84-85	0.33486754995416845
1105	86-87	0.2734128808641145
1105	88-89	0.2490953602025101
1105	90-91	0.2720316974369297
1105	92-93	0.3287327274644909
1105	94-95	0.3641067515694658
1105	96-97	0.32522008335482866
1105	98-99	0.28546235507095474
1105	100	0.3942234167735563
1106	1	0.10567076783375384
1106	2	0.15826888822031648
1106	3	0.1415850655776154
1106	4	0.10757235913199992
1106	5	0.07600034226980767
1106	6	0.16291517698682867
1106	7	0.10346348584609188
1106	8	0.21816480619725098
1106	9	0.25119241889299104
1106	10-11	0.24178682899285775
1106	12-13	0.23762342087003674
1106	14-15	0.308225774865754
1106	16-17	0.3209832543881532
1106	18-19	0.2863882796769417
1106	20-21	0.3098611114234231
1106	22-23	0.31977524965599713
1106	24-25	0.3445536800345579
1106	26-27	0.35258595643554713
1106	28-29	0.2825669656004379
1106	30-31	0.2803609229256452
1106	32-33	0.3524519511632249
1106	34-35	0.3927106187334104
1106	36-37	0.3688256121303013
1106	38-39	0.3104830422150542
1106	40-41	0.37720824551013976
1106	42-43	0.3755343184571487
1106	44-45	0.3531867003732998
1106	46-47	0.3701598609112864
1106	48-49	0.3674584730987078
1106	50-51	0.35640984753344185
1106	52-53	0.3262851503846491
1106	54-55	0.29424605144191673
1106	56-57	0.34312006843703813
1106	58-59	0.3239565182416939
1106	60-61	0.37454346205537803
1106	62-63	0.32326409800116096
1106	64-65	0.34052948191524735
1106	66-67	0.40585925687997104
1106	68-69	0.41367505259351844
1106	70-71	0.332388532629146
1106	72-73	0.3159528061233914
1106	74-75	0.3317414162347845
1106	76-77	0.3172340579961954
1106	78-79	0.3634026253343521
1106	80-81	0.33054181618950196
1106	82-83	0.25962674013942433
1106	84-85	0.32067911973823726
1106	86-87	0.2815217864397397
1106	88-89	0.2881889682251568
1106	90-91	0.33988839606971055
1106	92-93	0.34357464686996053
1106	94-95	0.31695017311375295
1106	96-97	0.3521176553586969
1106	98-99	0.30003223232994713
1106	100	0.2583025656660993
1107	1	0.0420730970461598
1107	2	0.0970533609497295
1107	3	0.05517558954889168
1107	4	0.04258483560537485
1107	5	-0.0037645234126841842
1107	6	0.06150236103968609
1107	7	0.05848442886038896
1107	8	0.028896603641861418
1107	9	0.1738815506563256
1107	10-11	0.13389049031497535
1107	12-13	0.19005287143823324
1107	14-15	0.17631484409444909
1107	16-17	0.13537637395316438
1107	18-19	0.17809595255579325
1107	20-21	0.16869705341238728
1107	22-23	0.21783931517931165
1107	24-25	0.23668262931044382
1107	26-27	0.18546885422592396
1107	28-29	0.19876325534698935
1107	30-31	0.2647717877107283
1107	32-33	0.19998426918842682
1107	34-35	0.27250234588745315
1107	36-37	0.24842799941059468
1107	38-39	0.21474308278136078
1107	40-41	0.17227880001781415
1107	42-43	0.15676252523566347
1107	44-45	0.19684901736302152
1107	46-47	0.20700246040329517
1107	48-49	0.22875061545840936
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2216	82-83	-0.6979366077695346
2216	84-85	-0.6735777155892322
2216	86-87	-0.702429353181941
2216	88-89	-0.678557512001646
2216	90-91	-0.7259103001326181
2216	92-93	-0.6956333841392777
2216	94-95	-0.7647949136192267
2216	96-97	-0.8079292909677669
2216	98-99	-0.8005888815040834
2216	100	-0.7584370752289402
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1511.0
1	392.0
2	269.0
3	258.0
4	202.0
5	266.0
6	476.0
7	1160.0
8	3109.0
9	3909.0
10	4257.0
11	5378.0
12	7001.0
13	9126.0
14	11210.0
15	12838.0
16	13699.0
17	15029.0
18	16333.0
19	18234.0
20	20389.0
21	22798.0
22	25212.0
23	29218.0
24	35153.0
25	42667.0
26	45231.0
27	43204.0
28	46952.0
29	57354.0
30	66826.0
31	77100.0
32	99013.0
33	132006.0
34	191361.0
35	292756.0
36	515333.0
37	1050054.0
38	1395884.0
39	462457.0
40	1076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99717469488926	19.69561643915996	13.297773579101207	24.00943528684957
2	14.406780371145631	20.992809535221628	40.729298818655494	23.87111127497725
3	18.21416853107205	26.77370637364737	30.445785298442445	24.56633979683813
4	11.987679070847229	17.91096094195896	39.95508195374853	30.146278033445277
5	12.869135011955638	38.97816388567846	35.44104693331411	12.711654169051787
6	29.30106248225442	38.35482360238166	19.24231740779894	13.101796507564986
7	25.833789070275685	33.504019914132876	23.149110841510407	17.513080174081036
8	24.51732096003165	35.877895225614814	21.396664924715438	18.2081188896381
9	25.854778121602056	16.729197525303206	20.930504430025707	36.48551992306903
10-11	23.608682707435495	26.901123177876617	29.23070057243164	20.259493542256248
12-13	25.074615796603826	24.62167829684606	28.35074499524489	21.952960911305222
14-15	22.747122638969525	26.027742677717757	26.140547088822792	25.084587594489932
16-17	21.884924114473304	28.703045457145425	27.449269094894568	21.962761333486704
18-19	21.69972918421183	28.957384881814168	28.128930147210184	21.21395578676381
20-21	22.657270181050336	28.299760140098794	27.15141174594921	21.89155793290166
22-23	22.405929232778597	28.085603502513813	26.464871677921924	23.043595586785663
24-25	22.3777236924354	28.193934957283396	26.90960304191814	22.518738308363066
26-27	22.77617308628231	27.79645630763874	27.05169765000397	22.375672956074983
28-29	22.303744912805314	28.04404069085277	27.40686327095723	22.245351125384683
30-31	22.578905715859594	28.178768934008836	27.179725024059397	22.062600326072175
32-33	22.248299991807187	28.486810936962087	26.885954610153178	22.37893446107755
34-35	22.47616387469727	28.246892848806194	27.181637749566356	22.095305526930183
36-37	22.8417356447744	27.823424425819788	27.105276805597246	22.229563123808564
38-39	23.002532649787852	27.624521598663275	27.3168695555966	22.056076195952265
40-41	22.667387043525597	27.582387602980575	27.79383853584556	21.95638681764827
42-43	22.564336882281218	27.77787149251215	27.250050317827412	22.40774130737922
44-45	22.409854103232643	28.224372620012755	27.177508257467448	22.188265019287158
46-47	22.307313697129246	27.991826524862905	26.945480062801323	22.755379715206523
48-49	22.526244276426404	27.78576585844148	27.153269130888557	22.53472073424356
50-51	22.528951151555372	27.845460587049693	27.00760097057564	22.617987290819293
52-53	22.06484229496774	28.083718176745943	27.11553160780287	22.73590792048345
54-55	22.228366414184382	27.86307704299854	27.25309428398767	22.655462258829406
56-57	22.480190082169234	27.842622491862233	27.04379247330519	22.63339495266334
58-59	22.21732539628402	27.827589534637724	27.315445109427817	22.639639959650435
60-61	22.453620097372777	27.734990546154098	27.308008866549628	22.503380489923494
62-63	22.58874717245894	27.668995480544716	27.111052526355806	22.631204820640534
64-65	22.08222092500191	27.69143089582703	27.39864470864568	22.827703470525385
66-67	22.42945410135221	27.44600243837139	27.31230591658716	22.812237543689236
68-69	22.49194739875308	27.700330067585266	27.060376172263844	22.74734636139781
70-71	22.1903952958741	27.725273129307087	27.08225534988906	23.002076224929745
72-73	22.341977546470474	27.556995925932252	27.212050296258212	22.888976231339065
74-75	22.395351149831967	27.952860981201543	26.996753806410588	22.6550340625559
76-77	22.446907653885916	27.49342848814047	27.14265446117794	22.91700939679567
78-79	22.23566579424335	27.487632519201977	27.44079076427301	22.83591092228167
80-81	22.235815362793996	27.772232242142376	27.400575403752953	22.591376991310682
82-83	22.340276371830644	27.509421766953835	27.346765036159216	22.8035368250563
84-85	22.504170260423976	27.4964741795938	27.020720265982924	22.9786352939993
86-87	22.387044240510264	27.623282167231643	27.30459382115123	22.68507977110687
88-89	22.74687982684979	27.104680707668503	27.302016708333245	22.846422757148467
90-91	22.617782998257475	27.128225063681356	27.42285152054621	22.831140417514966
92-93	22.489716498164167	27.51604094268638	27.246358012655055	22.747884546494397
94-95	22.113896099473767	27.525996086638777	27.41235668689705	22.947751126990404
96-97	22.368053932296085	27.231601824355632	27.13775632671309	23.26258791663519
98-99	22.440244649638323	27.377563318188823	27.136977411499352	23.045214620673494
100	22.633699081732406	27.665933099145786	26.741285440314705	22.959082378807103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3441.0
1	2291.0
2	1194.0
3	1179.5
4	1029.5
5	860.0
6	737.5
7	705.5
8	779.5
9	827.5
10	715.0
11	629.5
12	623.0
13	652.5
14	749.5
15	880.0
16	1038.0
17	1302.0
18	1714.0
19	2298.0
20	3206.0
21	4574.5
22	6582.5
23	9882.5
24	14387.5
25	18933.5
26	24302.0
27	31400.5
28	38596.5
29	47286.0
30	57404.0
31	67338.5
32	78784.5
33	89485.5
34	99850.0
35	111268.0
36	122431.0
37	135376.0
38	143220.0
39	144541.5
40	146210.5
41	147982.5
42	151171.5
43	160519.0
44	175375.5
45	187938.5
46	191077.0
47	195800.5
48	230221.0
49	233039.0
50	209279.5
51	196180.0
52	172048.0
53	158909.0
54	148984.5
55	134216.0
56	119531.5
57	104488.0
58	90026.0
59	78076.0
60	63613.0
61	49341.0
62	38684.5
63	30836.0
64	23022.0
65	15960.5
66	11776.0
67	9103.0
68	7001.0
69	5366.5
70	4346.5
71	3562.5
72	2864.5
73	2242.0
74	1804.0
75	1497.5
76	1123.0
77	764.5
78	515.0
79	360.5
80	259.5
81	183.0
82	133.0
83	87.0
84	61.0
85	49.5
86	45.5
87	40.0
88	29.5
89	22.0
90	14.0
91	14.0
92	21.0
93	18.0
94	13.0
95	18.0
96	15.5
97	9.5
98	9.0
99	9.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10105300708585277
2	0.06609163939714878
3	0.08279773006516422
4	0.08392821740360136
5	0.08493309503776769
6	0.0918206938219495
7	0.08815707744738471
8	0.08949691429293984
9	0.07517740800606947
10-11	0.08173004757886249
12-13	0.07150332415614877
14-15	0.08034834083188376
16-17	0.08206500679025126
18-19	0.08163584030065939
20-21	0.07323045758987218
22-23	0.07687313901372517
24-25	0.07937486562378512
26-27	0.07419346532261492
28-29	0.08233716114950465
30-31	0.08662882604542341
32-33	0.08842923180663809
34-35	0.09050179192710618
36-37	0.09114030792381604
38-39	0.09266855932577735
40-41	0.08790585803884313
42-43	0.09495046895336341
44-45	0.09115077539917193
46-47	0.08771744348243694
48-49	0.09756733779233827
50-51	0.09381998161492629
52-53	0.09920026394785858
54-55	0.09112984044846013
56-57	0.09754640284162647
58-59	0.10263359586459359
60-61	0.10118908426547946
62-63	0.10230910412856069
64-65	0.1038268880551661
66-67	0.10621347243631117
68-69	0.10364894097411581
70-71	0.10593085060170188
72-73	0.10330351428737114
74-75	0.10702993551407133
76-77	0.1040048351362164
78-79	0.11131113293463418
80-81	0.10666357387661485
82-83	0.111018043624669
84-85	0.10711367531691851
86-87	0.10372221330160712
88-89	0.10238237645605199
90-91	0.10249751868496688
92-93	0.09662526501030733
94-95	0.09606002134108875
96-97	0.09411307092489146
98-99	0.10937464999379279
100	0.24826758049122186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4776701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.10515672116207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71402146314044	44.432016773311396
2	14.973240215911563	18.298843800337654
3	5.656852877054016	10.369886448028264
4	2.6407384148393898	6.454509387934161
5	1.4162719492027724	4.327075970791054
6	0.8038217765029644	2.9470593377457925
7	0.5157873712133769	2.2062087706952713
8	0.32718932016198876	1.5994363748791036
9	0.2249002953391743	1.2368311014002291
>10	0.7173148400248652	6.420527187965916
>50	0.007462175596753515	0.2974640967915983
>100	0.0020205671157385477	0.2082135937011835
>500	0.0	0.0
>1k	2.7052421208857247E-4	0.46252127405562016
>5k	0.0	0.0
>10k+	1.0820968483542899E-4	0.7394058823626837
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	12316	0.2578348529665139	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	10049	0.21037531970286605	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.280485213539637E-5	0.0
3	0.0	0.0	0.0	6.280485213539637E-5	0.0
4	0.0	0.0	0.0	8.373980284719517E-5	0.0
5	0.0	0.0	0.0	1.2560970427079275E-4	0.0
6	0.0	0.0	0.0	1.2560970427079275E-4	2.0934950711798793E-5
7	0.0	0.0	0.0	1.4654465498259155E-4	2.0934950711798793E-5
8	0.0	0.0	0.0	1.4654465498259155E-4	2.0934950711798793E-5
9	0.0	0.0	0.0	1.8841455640618914E-4	2.0934950711798793E-5
10-11	0.0	0.0	0.0	2.930893099651831E-4	2.0934950711798793E-5
12-13	0.0	0.0	0.0	2.930893099651831E-4	4.1869901423597585E-5
14-15	0.0	0.0	0.0	3.454266867446801E-4	5.2337376779496977E-5
16-17	0.0	0.0	0.0	3.768291128123783E-4	6.280485213539637E-5
18-19	0.0	0.0	0.0	3.97764063524177E-4	6.280485213539637E-5
20-21	0.0	0.0	0.0	4.396339649477746E-4	6.280485213539637E-5
22-23	0.0	0.0	0.0	5.966460952862656E-4	6.280485213539637E-5
24-25	0.0	0.0	0.0	8.583329791837504E-4	6.280485213539637E-5
26-27	0.0	0.0	0.0	0.0017166659583675008	6.280485213539637E-5
28-29	0.0	0.0	0.0	0.004699896434798829	6.280485213539637E-5
30-31	0.0	0.0	0.0	0.012874994687756257	6.280485213539637E-5
32-33	0.0	0.0	0.0	0.031214011511291995	6.280485213539637E-5
34-35	0.0	0.0	0.0	0.06168483227231514	6.280485213539637E-5
36-37	0.0	0.0	0.0	0.11313247364656068	6.280485213539637E-5
38-39	0.0	0.0	0.0	0.18615358172931484	6.280485213539637E-5
40-41	0.0	0.0	0.0	0.26648098761048683	6.280485213539637E-5
42-43	0.0	0.0	0.0	0.346264084773152	6.280485213539637E-5
44-45	0.0	0.0	0.0	0.4453596739674516	6.280485213539637E-5
46-47	0.0	0.0	0.0	0.5707495612557705	6.280485213539637E-5
48-49	0.0	0.0	2.0934950711798793E-5	0.7099879184399442	6.280485213539637E-5
50-51	0.0	0.0	2.0934950711798793E-5	0.85483684241488	6.280485213539637E-5
52-53	0.0	0.0	2.0934950711798793E-5	1.000617371696491	8.373980284719517E-5
54-55	0.0	0.0	2.0934950711798793E-5	1.1631877314489645	8.373980284719517E-5
56-57	0.0	0.0	2.0934950711798793E-5	1.355004217345821	9.420727820309456E-5
58-59	0.0	0.0	2.0934950711798793E-5	1.580578311265453	1.0467475355899395E-4
60-61	0.0	0.0	2.0934950711798793E-5	1.8282283107106765	1.0467475355899395E-4
62-63	0.0	0.0	2.0934950711798793E-5	2.073418453447264	1.0467475355899395E-4
64-65	0.0	0.0	2.0934950711798793E-5	2.336162552355695	1.0467475355899395E-4
66-67	0.0	0.0	2.0934950711798793E-5	2.6265093837776323	1.0467475355899395E-4
68-69	0.0	0.0	2.0934950711798793E-5	2.9560778453581245	1.0467475355899395E-4
70-71	0.0	0.0	2.0934950711798793E-5	3.3175825742494665	1.0467475355899395E-4
72-73	0.0	0.0	2.0934950711798793E-5	3.6832952282338796	1.0467475355899395E-4
74-75	0.0	0.0	2.0934950711798793E-5	4.0446638799455945	1.0467475355899395E-4
76-77	0.0	0.0	2.0934950711798793E-5	4.4371523358903975	1.0467475355899395E-4
78-79	0.0	0.0	2.0934950711798793E-5	4.882239855498597	1.0467475355899395E-4
80-81	0.0	0.0	2.0934950711798793E-5	5.356290879416568	1.0467475355899395E-4
82-83	0.0	0.0	2.0934950711798793E-5	5.847341083312521	1.0467475355899395E-4
84-85	0.0	0.0	2.0934950711798793E-5	6.348858762564372	1.0467475355899395E-4
86-87	0.0	0.0	2.0934950711798793E-5	6.874514439986928	1.0467475355899395E-4
88	0.0	0.0	2.0934950711798793E-5	7.283185612831953	1.0467475355899395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1835	0.0	20.244444	1
CATGGGG	8195	0.0	17.897335	4
CTCGTCG	875	0.0	17.184729	9
ACCGTAT	310	1.2644523E-7	16.674564	8
GTATAAG	2660	0.0	16.086983	1
GTATAAT	2315	0.0	16.046892	1
GTACATG	24975	0.0	15.985162	1
GCATCGT	1670	0.0	15.75778	8
TACATGG	24385	0.0	15.634387	2
GTAGGAC	2135	0.0	15.633033	3
TAGTACT	2490	0.0	15.480939	4
ACATGGG	23300	0.0	15.33347	3
CTGTGCG	2075	0.0	15.172511	9
ATGGGGG	5745	0.0	15.05556	5
CGTTAAC	285	1.742015E-5	14.849522	1
CTAGTAC	2345	0.0	14.834457	3
GTGTAGC	2825	0.0	14.814479	1
TACTAGG	2670	0.0	14.789409	2
CGATAAT	890	0.0	14.787781	66-67
GCTATTG	2690	0.0	14.6740675	8
>>END_MODULE