FastQCFastQC Report
Mon 16 Jan 2017
ERR1146589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4776701
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144270.30202853391912116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140090.2932777245215893No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA120690.25266392014069966No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA98480.20616739460979452No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG95050.1989867065156475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75830.15874973124757025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT158200.022.57753212-13
GTACTTT176300.020.17961714-15
AGTACTT171650.018.98768612-13
CATGGGG79350.017.6602944
CTGTGCG21100.017.5960039
CGTTAAC3752.0008883E-1017.5455179
ACTTTTT206750.017.32123616-17
CTAGTAC23450.016.44373
TACTTTT203850.015.95382214-15
TACCTGG45000.015.5709812
TAACGCC6500.015.1926844
GTACATG242250.014.9479891
ACATGGG228250.014.689513
TAGTACT25750.014.60974
TACATGG240000.014.59779552
ACTGTGC38950.014.4793068
ATGGGGG55700.014.4288945
CCTAGTA28100.014.2250832
TATACCG4302.305751E-814.2091145
GTACTAG16550.014.2078351
Produced by FastQC (version 0.11.5)