FastQCFastQC Report
Mon 16 Jan 2017
ERR1146588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983768
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.036.4362261
GTATCAA55050.033.7452131
ATCAACG56900.030.6539613
CAACGCA57700.030.391535
TATCAAC68050.027.220652
TCAACGC69250.025.7981644
AACGCAG70850.025.6684476
GTACTAG18150.025.6525751
GTGATCG11850.024.586488
CGCAGAG71700.024.4462768
ACGCAGA72450.024.3877937
GTACATG118650.023.7824121
TACATGG116650.023.295582
TGATCGC12850.023.0379629
ACATGGG115400.022.7735633
GTATAAG21300.022.3004781
CATGGGG90200.021.3177684
CTAACGC7650.020.8950043
GCATCGT16400.019.7709838
GCAGAGT85250.019.7330999
Produced by FastQC (version 0.11.5)